World-2DPAGE Repository

RecName: Full=Neurofilament medium polypeptide; Short=NF-M; AltName: Full=160 kDa neurofilament protein; AltName: Full=Neurofilament 3; AltName: Full=Neurofilament triplet M protein;.

Gene name

Name=Nefm
Synonyms=Nef3, Nfm

Annotated species

Rattus norvegicus (Rat) [TaxID: 10116]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	MAPPING ON GEL PubMed=19343716; DOI=10.1002/pmic.200800664; [NCBI, EBI, Israel, Japan] Maurya D.K., Sundaram C.S., Bhargava P. ''Proteome profile of the mature rat olfactory bulb'' Proteomics 9(1):2593-2599 (2009)

2D PAGE maps for identified proteins

How to interpret a protein

OB21DSUB {2-DE gel for Olfactory bulb proteome}
Rattus norvegicus (Rat)
Tissue: Olfactory bulb

map experimental info

OB21DSUB

MAP LOCATIONS:

SPOT 68:

pI=4.77; Mw=95000

[identification data]

MAPPING (identification):

SPOT 68: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Purnima Bhargava, Centre for Cellular and Molecular Biology, India

Cross-references

UniProtKB/Swiss-Prot

P12839; NFM_RAT.

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry name	NFM_RAT
Primary accession number	P12839
Secondary accession number(s)	Q63370
Sequence was last modified on	January 23, 2007 (version 4)
Annotations were last modified on	November 16, 2011 (version 108)
Name and origin of the protein
Description	RecName: Full=Neurofilament medium polypeptide; Short=NF-M; AltName: Full=160 kDa neurofilament protein; AltName: Full=Neurofilament 3; AltName: Full=Neurofilament triplet M protein;
Gene name	Name=Nefm Synonyms=Nef3, Nfm
Encoded on	Name=Nefm; Synonyms=Nef3, Nfm
Keywords	Acetylation; Coiled coil; Complete proteome; Direct protein sequencing; Glycoprotein; Intermediate filament; Phosphoprotein; Reference proteome.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	M18628; AAA41696.1; -; mRNA
EMBL	Z12152; CAA78136.1; -; mRNA
IPI	IPI00325609; -; .
PIR	A45669; A45669; .
RefSeq	NP_058725.1; NM_017029.1; .
UniGene	Rn.10971; -; .
ProteinModelPortal	P12839; -; .
SMR	P12839; 98-135; 329-406; .
DIP	DIP-208N; -; .
STRING	P12839; -; .
GlycoSuiteDB	P12839; -; .
PhosphoSite	P12839; -; .
World-2DPAGE	0004:P12839; -; .
PRIDE	P12839; -; .
GeneID	24588; -; .
KEGG	rno:24588; -; .
UCSC	NM_017029; rat; .
CTD	4741; -; .
RGD	3160; Nefm; .
eggNOG	roNOG11778; -; .
GeneTree	ENSGT00560000076592; -; .
HOVERGEN	HBG013015; -; .
InParanoid	P12839; -; .
OrthoDB	EOG4VMFFD; -; .
NextBio	603770; -; .
ArrayExpress	P12839; -; .
Genevestigator	P12839; -; .
GermOnline	ENSRNOG00000013916; Rattus norvegicus; .
GO	GO:0030424; C:axon; IDA:BHF-UCL; .
GO	GO:0005882; C:intermediate filament; IEA:UniProtKB-KW; .
GO	GO:0005198; F:structural molecule activity; IEA:InterPro; .
InterPro	IPR016044; F; .
InterPro	IPR001664; IF; .
InterPro	IPR006821; Intermed_filament_DNA-bd; .
InterPro	IPR018039; Intermediate_filament_CS; .
InterPro	IPR002957; Keratin_I; .
KO	K04573; -; .
PANTHER	PTHR23239; IF; 1; .
Pfam	PF00038; Filament; 1; .
Pfam	PF04732; Filament_head; 1; .
PRINTS	PR01248; TYPE1KERATIN; .
PROSITE	PS00226; IF; 1; .

World-2DPAGE Repository (search AC)

Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server

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