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Searching in 'World-2DPAGE Repository [0030]' for entry matching: P13264




World-2DPAGE Repository (0030):  P13264


P13264


General information about the entry
View entry in simple text format
Entry nameGLSK_RAT
Primary accession numberP13264
integrated into World-2DPAGE Repository (0030) on November 9, 2010 (release 1)
2D Annotations were last modified onJune 21, 2011 (version 2)
General Annotations were last modified on November 23, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Glutaminase kidney isoform, mitochondrial; Short=GLS; EC=3.5.1.2; AltName: Full=K-glutaminase; AltName: Full=L-glutamine amidohydrolase; Contains: RecName: Full=Glutaminase kidney isoform 68 kDa chain; Contains: RecName: Full=Glutaminase kidney isoform 65 kDa chain; Flags: Precursor;.
Gene nameName=Gls
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000006;
D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C.
''Two-dimensional gel proteome reference map of INS-1E cells''
Proteomics 11(7):1365-1369 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7}
Rattus norvegicus (Rat)
RAT_INS1E_4-7
  map experimental info
 
RAT_INS1E_4-7

MAP LOCATIONS:
pI=6.42; Mw=73501  [identification data]
pI=6.60; Mw=71991  [identification data]
pI=6.30; Mw=68022  [identification data]

IDENTIFICATION: SPOT 700: Mascot protein score=65. Number of peptides=4 [1]
SPOT 740: Mascot protein score=714. Number of peptides=11 [1]; SPOT 838: Mascot protein score=128. Number of peptides=3 [1].
MAPPING (identification):
SPOT 700: Tandem mass spectrometry [1];
SPOT 740: Tandem mass spectrometry [1];
SPOT 838: Tandem mass spectrometry [1].

Copyright
Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium
Cross-references
UniProtKB/Swiss-ProtP13264; GLSK_RAT.
World-2DPAGE RepositoryP13264; GLSK_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameGLSK_RAT
Primary accession numberP13264
Secondary accession number(s) Q8R421
Sequence was last modified on December 1, 1992 (version 2)
Annotations were last modified on November 16, 2011 (version 101)
Name and origin of the protein
DescriptionRecName: Full=Glutaminase kidney isoform, mitochondrial; Short=GLS; EC=3.5.1.2; AltName: Full=K-glutaminase; AltName: Full=L-glutamine amidohydrolase; Contains: RecName: Full=Glutaminase kidney isoform 68 kDa chain; Contains: RecName: Full=Glutaminase kidney isoform 65 kDa chain; Flags: Precursor;
Gene nameName=Gls
Encoded onName=Gls
KeywordsAcetylation; Alternative splicing; ANK repeat; Complete proteome; Direct protein sequencing; Hydrolase; Mitochondrion; Reference proteome; Transit peptide.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM65150; AAA41247.1; -; mRNA
EMBLAF302091; AAG30873.1; -; Genomic_DNA
EMBLAY083459; AAM00020.1; -; mRNA
EMBLM22586; AAA41234.1; -; mRNA
IPIIPI00199465; -; .
PIRA41009; A41009; .
RefSeqNP_001103438.1; NM_001109968.1; .
RefSeqNP_036701.2; NM_012569.2; .
UniGeneRn.5762; -; .
ProteinModelPortalP13264; -; .
SMRP13264; 227-536; .
STRINGP13264; -; .
PhosphoSiteP13264; -; .
PRIDEP13264; -; .
GeneID24398; -; .
KEGGrno:24398; -; .
CTD2744; -; .
RGD2707; Gls; .
GeneTreeENSGT00390000010463; -; .
HOVERGENHBG005856; -; .
OrthoDBEOG4ZS92W; -; .
PhylomeDBP13264; -; .
NextBio603191; -; .
GenevestigatorP13264; -; .
GOGO:0005739; C:mitochondrion; IDA:RGD; .
GOGO:0004359; F:glutaminase activity; TAS:RGD; .
GOGO:0006543; P:glutamine catabolic process; TAS:RGD; .
InterProIPR002110; Ankyrin_rpt; .
InterProIPR020683; Ankyrin_rpt-contain_dom; .
InterProIPR012338; Beta-lactam/transpept-like; .
InterProIPR015868; Glutaminase; .
Gene3DG3DSA:1.25.40.20; ANK; 1; .
Gene3DG3DSA:3.40.710.20; Glutaminase_core; 2; .
KOK01425; -; .
PANTHERPTHR12544; Glutaminase; 1; .
PfamPF00023; Ank; 1; .
PfamPF04960; Glutaminase; 1; .
SMARTSM00248; ANK; 2; .
SUPFAMSSF48403; ANK; 1; .
SUPFAMSSF56601; PBP_transp_fold; 1; .
TIGRFAMsTIGR03814; Gln_ase; 1; .
PROSITEPS50297; ANK_REP_REGION; 1; .
PROSITEPS50088; ANK_REPEAT; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server