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World-2DPAGE Repository  Search by  [accession number]  [description, ID or gene]  [author names]  [spot ID / serial number]  [identification methods]  [pI / Mw range]  [combined fields]  Maps  [experimental info]  [protein list]  [graphical interface]      Databases 0001 0002 0003 0004 0005 0006 0007 0008 0009 0010 0011 0012 0014 0017 0018 0019 0020 0021 0022 0023 0024 0026 0027 0029 0030 0031 0032 0034 0035 0036 0037 0038 0039 0040 0042 0043 0044 0046 0047 0050 0051 0053 0054 0056 0059 0061 0062 0063 0066 0068 0069 0076 0077 0080 0084 0085 0088 use 'Ctrl' to select several databases Select Remote Interfaces [All Interfaces] SWISS-2DPAGE World-2DPAGE Portal Exclude local DBs has only effect if a remote interface is selected

Searching in 'World-2DPAGE Repository [0022]' for entry matching: P14408 World-2DPAGE Repository (0022):  P14408 P14408 General information about the entry View entry in simple text format Entry name FUMH_RAT Primary accession number P14408 integrated into World-2DPAGE Repository (0022) on  May 7, 2010 (release 1) 2D Annotations were last modified on June 22, 2011 (version 2) General Annotations were last modified on  November 24, 2011 (version 2) Name and origin of the protein Description RecName: Full=Fumarate hydratase, mitochondrial; Short=Fumarase; EC=4.2.1.2; Flags: Precursor;. Gene name Name=Fh Synonyms=Fh1 Annotated species Rattus [TaxID: 10114] Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae. References [1]   2D-PAGE GEL CHARACTERIZATION DOI=10.1002/pmic.201000593; Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L. ''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity'' Proteomics 11(16):3369-3379 (2011) 2D PAGE maps for identified proteins How to interpret a protein HCR_LCR_HEART {HCR LCR Cardiac Map} Rattus Tissue: Heart   map experimental info   HCR_LCR_HEART MAP LOCATIONS: SPOT 5: pI=9.06; Mw=54714   [identification data] SPOT 6: pI=9.06; Mw=54714   [identification data] SPOT 8: pI=9.06; Mw=54714   [identification data] SPOT 254: pI=9.06; Mw=54714   [identification data] IDENTIFICATION: SPOT 5: SeqCov=25.6%. Peptides MS=10/20. Mascot PMF Score=76 [1] SPOT 6: SeqCov=17.8%. Peptides MS=10/20. Mascot PMF Score=62 [1]; SPOT 8: SeqCov=25.6%. Peptides MS=10/20. Mascot PMF Score=76 [1]; SPOT 254: SeqCov=23.5%. Peptides MS=9/20. Mascot PMF Score=66 [1]. PEPTIDE EVIDENCE: SPOT 5: 341-FLGSGPR-347 (37) / 64-YYGAQTVR-71 (27) / 419-LLGDASVSFTENCVVGIQANTER-441 (54) [1] SPOT 6: 341-FLGSGPR-347 (16) / 64-YYGAQTVR-71 (23) / 419-LLGDASVSFTENCVVGIQANTER-441 (32) [1]; SPOT 8: 341-FLGSGPR-347 (22) / 266-IYELAAGGTAVGTGLNTR-283 (75) / 419-LLGDASVSFTENCVVGIQANTER-441 (98) [1]; SPOT 254: 341-FLGSGPR-347 (29) / 64-YYGAQTVR-71 (20) / 419-LLGDASVSFTENCVVGIQANTER-441 (80) [1]. MAPPING (identification): SPOT 5: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1]; SPOT 6: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1]; SPOT 8: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1]; SPOT 254: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1]. Copyright Data from Dr. Jatin Burniston, Liverpool John Moores University, UK Cross-references UniProtKB/Swiss-Prot P14408; FUMH_RAT. 2D PAGE maps for identified proteins How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed. External data extracted from UniProtKB/Swiss-Prot Extracted from UniProtKB/Swiss-Prot, release: 2011_11 Entry name FUMH_RAT Primary accession number P14408 Sequence was last modified on  January 1, 1990 (version 1) Annotations were last modified on  November 16, 2011 (version 113) Name and origin of the protein Description RecName: Full=Fumarate hydratase, mitochondrial; Short=Fumarase; EC=4.2.1.2; Flags: Precursor; Gene name Name=Fh Synonyms=Fh1 Encoded on Name=Fh; Synonyms=Fh1 Keywords Acetylation; Alternative initiation; Complete proteome; Cytoplasm; Direct protein sequencing; Lyase; Mitochondrion; Phosphoprotein; Reference proteome; Transit peptide; Tricarboxylic acid cycle. Copyright Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License Cross-references EMBL J04473; AAA41177.1; -; mRNA IPI IPI00231611; -; . IPI IPI00760133; -; . PIR A31424; UFRT; . UniGene Rn.29782; -; . ProteinModelPortal P14408; -; . SMR P14408; 46-507; . IntAct P14408; 1; . STRING P14408; -; . PhosphoSite P14408; -; . PRIDE P14408; -; . UCSC NM_017005; rat; . RGD 2614; Fh1; . eggNOG roNOG08582; -; . HOVERGEN HBG002183; -; . InParanoid P14408; -; . OrthoDB EOG4F1X31; -; . PhylomeDB P14408; -; . ArrayExpress P14408; -; . Genevestigator P14408; -; . GermOnline ENSRNOG00000003653; Rattus norvegicus; . GO GO:0005739; C:mitochondrion; IDA:RGD; . GO GO:0045239; C:tricarboxylic acid cycle enzyme complex; IEA:InterPro; . GO GO:0004333; F:fumarate hydratase activity; IDA:RGD; . GO GO:0006106; P:fumarate metabolic process; IDA:RGD; . GO GO:0006108; P:malate metabolic process; IDA:RGD; . GO GO:0006099; P:tricarboxylic acid cycle; IDA:RGD; . InterPro IPR005677; Fum_hydII; . InterPro IPR018951; Fumarase_C_C; . InterPro IPR000362; Fumarate_lyase; . InterPro IPR020557; Fumarate_lyase_CS; . InterPro IPR008948; L-Aspartase-like; . InterPro IPR024083; L-Aspartase-like_N; . InterPro IPR022761; Lyase1_N; . Gene3D G3DSA:1.10.275.10; G3DSA:1.10.275.10; 1; . Pfam PF10415; FumaraseC_C; 1; . Pfam PF00206; Lyase_1; 1; . PRINTS PR00149; FUMRATELYASE; . SUPFAM SSF48557; L-Aspartase-like; 1; . TIGRFAMs TIGR00979; FumC_II; 1; . PROSITE PS00163; FUMARATE_LYASES; 1; . World-2DPAGE Repository (search AC)