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Searching in 'World-2DPAGE Repository [0022]' for entry matching: P14408




World-2DPAGE Repository (0022):  P14408

P14408


General information about the entry
View entry in simple text format
Entry nameFUMH_RAT
Primary accession numberP14408
integrated into World-2DPAGE Repository (0022) on May 7, 2010 (release 1)
2D Annotations were last modified onJune 22, 2011 (version 2)
General Annotations were last modified on November 24, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Fumarate hydratase, mitochondrial; Short=Fumarase; EC=4.2.1.2; Flags: Precursor;.
Gene nameName=Fh
Synonyms=Fh1
Annotated speciesRattus [TaxID: 10114]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000593;
Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L.
''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity''
Proteomics 11(16):3369-3379 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

HCR_LCR_HEART {HCR LCR Cardiac Map}
Rattus
Tissue: Heart
HCR_LCR_HEART
  map experimental info 		 
HCR_LCR_HEART

MAP LOCATIONS:
pI=9.06; Mw=54714  [identification data]
pI=9.06; Mw=54714  [identification data]
pI=9.06; Mw=54714  [identification data]
pI=9.06; Mw=54714  [identification data]

IDENTIFICATION: SPOT 5: SeqCov=25.6%. Peptides MS=10/20. Mascot PMF Score=76 [1]
SPOT 6: SeqCov=17.8%. Peptides MS=10/20. Mascot PMF Score=62 [1]; SPOT 8: SeqCov=25.6%. Peptides MS=10/20. Mascot PMF Score=76 [1]; SPOT 254: SeqCov=23.5%. Peptides MS=9/20. Mascot PMF Score=66 [1].
PEPTIDE EVIDENCE: SPOT 5: 341-FLGSGPR-347 (37) / 64-YYGAQTVR-71 (27) / 419-LLGDASVSFTENCVVGIQANTER-441 (54) [1]
SPOT 6: 341-FLGSGPR-347 (16) / 64-YYGAQTVR-71 (23) / 419-LLGDASVSFTENCVVGIQANTER-441 (32) [1]; SPOT 8: 341-FLGSGPR-347 (22) / 266-IYELAAGGTAVGTGLNTR-283 (75) / 419-LLGDASVSFTENCVVGIQANTER-441 (98) [1]; SPOT 254: 341-FLGSGPR-347 (29) / 64-YYGAQTVR-71 (20) / 419-LLGDASVSFTENCVVGIQANTER-441 (80) [1].
MAPPING (identification):
SPOT 5: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 6: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 8: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 254: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright
Data from Dr. Jatin Burniston, Liverpool John Moores University, UK
Cross-references
UniProtKB/Swiss-ProtP14408; FUMH_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameFUMH_RAT
Primary accession numberP14408
Sequence was last modified on January 1, 1990 (version 1)
Annotations were last modified on November 16, 2011 (version 113)
Name and origin of the protein
DescriptionRecName: Full=Fumarate hydratase, mitochondrial; Short=Fumarase; EC=4.2.1.2; Flags: Precursor;
Gene nameName=Fh
Synonyms=Fh1
Encoded onName=Fh; Synonyms=Fh1
KeywordsAcetylation; Alternative initiation; Complete proteome; Cytoplasm; Direct protein sequencing; Lyase; Mitochondrion; Phosphoprotein; Reference proteome; Transit peptide; Tricarboxylic acid cycle.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLJ04473; AAA41177.1; -; mRNA
IPIIPI00231611; -; .
IPIIPI00760133; -; .
PIRA31424; UFRT; .
UniGeneRn.29782; -; .
ProteinModelPortalP14408; -; .
SMRP14408; 46-507; .
IntActP14408; 1; .
STRINGP14408; -; .
PhosphoSiteP14408; -; .
PRIDEP14408; -; .
UCSCNM_017005; rat; .
RGD2614; Fh1; .
eggNOGroNOG08582; -; .
HOVERGENHBG002183; -; .
InParanoidP14408; -; .
OrthoDBEOG4F1X31; -; .
PhylomeDBP14408; -; .
ArrayExpressP14408; -; .
GenevestigatorP14408; -; .
GermOnlineENSRNOG00000003653; Rattus norvegicus; .
GOGO:0005739; C:mitochondrion; IDA:RGD; .
GOGO:0045239; C:tricarboxylic acid cycle enzyme complex; IEA:InterPro; .
GOGO:0004333; F:fumarate hydratase activity; IDA:RGD; .
GOGO:0006106; P:fumarate metabolic process; IDA:RGD; .
GOGO:0006108; P:malate metabolic process; IDA:RGD; .
GOGO:0006099; P:tricarboxylic acid cycle; IDA:RGD; .
InterProIPR005677; Fum_hydII; .
InterProIPR018951; Fumarase_C_C; .
InterProIPR000362; Fumarate_lyase; .
InterProIPR020557; Fumarate_lyase_CS; .
InterProIPR008948; L-Aspartase-like; .
InterProIPR024083; L-Aspartase-like_N; .
InterProIPR022761; Lyase1_N; .
Gene3DG3DSA:1.10.275.10; G3DSA:1.10.275.10; 1; .
PfamPF10415; FumaraseC_C; 1; .
PfamPF00206; Lyase_1; 1; .
PRINTSPR00149; FUMRATELYASE; .
SUPFAMSSF48557; L-Aspartase-like; 1; .
TIGRFAMsTIGR00979; FumC_II; 1; .
PROSITEPS00163; FUMARATE_LYASES; 1; .


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