World-2DPAGE Repository
Home
|
Contact
Due to maintenance work, this service and ftp://ftp.expasy.org will be unavailable from Tuesday August 23rd 18:00 until Wednesday August 24th 08:00
CEST
. Apologies for the inconvenience.
!!This displayer contains script commands. You should enable JavaScripting within your browser preferences, or use another browser!
World-2DPAGE Repository
Search by
[accession number]
[description, ID or gene]
[author names]
[spot ID / serial number]
[identification methods]
[pI / Mw range]
[combined fields]
Maps
[experimental info]
[protein list]
[graphical interface]
Databases
0001
0002
0003
0004
0005
0006
0007
0008
0009
0010
0011
0012
0014
0017
0018
0019
0020
0021
0022
0023
0024
0026
0027
0029
0030
0031
0032
0034
0035
0036
0037
0038
0039
0040
0042
0043
0044
0046
0047
0050
0051
0053
0054
0056
0059
0061
0062
0063
0066
0068
0069
0076
0077
0080
0084
0085
0088
use 'Ctrl' to select several
databases
Select Remote Interfaces
[All Interfaces]
SWISS-2DPAGE
World-2DPAGE Portal
Exclude local DBs
has only effect if a remote
interface is selected
Searching in 'World-2DPAGE Repository [0022]' for entry
matching:
P14408
World-2DPAGE Repository
(0022):
P14408
P14408
General information about the entry
View entry in simple text format
Entry name
FUMH_RAT
Primary accession number
P14408
integrated into World-2DPAGE Repository (0022) on
May 7, 2010 (release 1)
2D Annotations were last modified on
June 22, 2011 (version 2)
General Annotations were last modified on
November 24, 2011 (version 2)
Name and origin of the protein
Description
RecName: Full=Fumarate hydratase, mitochondrial; Short=Fumarase; EC=4.2.1.2; Flags: Precursor;.
Gene name
Name=Fh
Synonyms=Fh1
Annotated species
Rattus [TaxID:
10114
]
Taxonomy
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae.
References
[1]
2D-PAGE GEL CHARACTERIZATION
DOI=
10.1002/pmic.201000593
;
Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L.
''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity''
Proteomics 11(16):3369-3379 (2011)
2D PAGE maps for identified proteins
How to interpret a protein
HCR_LCR_HEART
{HCR LCR Cardiac Map}
Rattus
Tissue: Heart
map experimental info
HCR_LCR_HEART
MAP LOCATIONS:
SPOT 5
:
pI=9.06; Mw=54714
[identification data]
SPOT 6
:
pI=9.06; Mw=54714
[identification data]
SPOT 8
:
pI=9.06; Mw=54714
[identification data]
SPOT 254
:
pI=9.06; Mw=54714
[identification data]
IDENTIFICATION:
SPOT 5: SeqCov=25.6%. Peptides MS=10/20. Mascot PMF Score=76 [
1
]
SPOT 6: SeqCov=17.8%. Peptides MS=10/20. Mascot PMF Score=62 [
1
]; SPOT 8: SeqCov=25.6%. Peptides MS=10/20. Mascot PMF Score=76 [
1
]; SPOT 254: SeqCov=23.5%. Peptides MS=9/20. Mascot PMF Score=66 [
1
].
PEPTIDE EVIDENCE:
SPOT 5: 341-FLGSGPR-347 (37) / 64-YYGAQTVR-71 (27) / 419-LLGDASVSFTENCVVGIQANTER-441 (54) [
1
]
SPOT 6: 341-FLGSGPR-347 (16) / 64-YYGAQTVR-71 (23) / 419-LLGDASVSFTENCVVGIQANTER-441 (32) [
1
]; SPOT 8: 341-FLGSGPR-347 (22) / 266-IYELAAGGTAVGTGLNTR-283 (75) / 419-LLGDASVSFTENCVVGIQANTER-441 (98) [
1
]; SPOT 254: 341-FLGSGPR-347 (29) / 64-YYGAQTVR-71 (20) / 419-LLGDASVSFTENCVVGIQANTER-441 (80) [
1
].
MAPPING (identification):
SPOT
5
:
Peptide mass fingerprinting
[
1
];
Tandem mass spectrometry
[
1
];
SPOT
6
:
Peptide mass fingerprinting
[
1
];
Tandem mass spectrometry
[
1
];
SPOT
8
:
Peptide mass fingerprinting
[
1
];
Tandem mass spectrometry
[
1
];
SPOT
254
:
Peptide mass fingerprinting
[
1
];
Tandem mass spectrometry
[
1
].
Copyright
Data from Dr. Jatin Burniston, Liverpool John Moores University, UK
Cross-references
UniProtKB/Swiss-Prot
P14408; FUMH_RAT.
2D PAGE maps for identified proteins
How to interpret a protein map
You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
Warning 1
: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
Warning 2
: the 2D PAGE map is built on demand. This may take some few seconds to be computed.
External data extracted from
UniProtKB/Swiss-Prot
Extracted from
UniProtKB/Swiss-Prot
, release:
2011_11
Entry name
FUMH_RAT
Primary accession number
P14408
Sequence was last modified on
January 1, 1990 (version 1)
Annotations were last modified on
November 16, 2011 (version 113)
Name and origin of the protein
Description
RecName: Full=Fumarate hydratase, mitochondrial; Short=Fumarase; EC=4.2.1.2; Flags: Precursor;
Gene name
Name=Fh
Synonyms=Fh1
Encoded on
Name=Fh; Synonyms=Fh1
Keywords
Acetylation
;
Alternative initiation
;
Complete proteome
;
Cytoplasm
;
Direct protein sequencing
;
Lyase
;
Mitochondrion
;
Phosphoprotein
;
Reference proteome
;
Transit peptide
;
Tricarboxylic acid cycle
.
Copyright
Copyrighted by the UniProt Consortium, see
https://www.uniprot.org/help/license
. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL
J04473; AAA41177.1
; -; mRNA
IPI
IPI00231611; -
; .
IPI
IPI00760133; -
; .
PIR
A31424; UFRT
; .
UniGene
Rn.29782; -
; .
ProteinModelPortal
P14408; -
; .
SMR
P14408; 46-507
; .
IntAct
P14408; 1
; .
STRING
P14408; -
; .
PhosphoSite
P14408; -
; .
PRIDE
P14408; -
; .
UCSC
NM_017005; rat
; .
RGD
2614; Fh1
; .
eggNOG
roNOG08582; -
; .
HOVERGEN
HBG002183; -
; .
InParanoid
P14408; -
; .
OrthoDB
EOG4F1X31; -
; .
PhylomeDB
P14408; -
; .
ArrayExpress
P14408; -
; .
Genevestigator
P14408; -
; .
GermOnline
ENSRNOG00000003653; Rattus norvegicus
; .
GO
GO:0005739; C:mitochondrion
; IDA:RGD; .
GO
GO:0045239; C:tricarboxylic acid cycle enzyme complex
; IEA:InterPro; .
GO
GO:0004333; F:fumarate hydratase activity
; IDA:RGD; .
GO
GO:0006106; P:fumarate metabolic process
; IDA:RGD; .
GO
GO:0006108; P:malate metabolic process
; IDA:RGD; .
GO
GO:0006099; P:tricarboxylic acid cycle
; IDA:RGD; .
InterPro
IPR005677; Fum_hydII
; .
InterPro
IPR018951; Fumarase_C_C
; .
InterPro
IPR000362; Fumarate_lyase
; .
InterPro
IPR020557; Fumarate_lyase_CS
; .
InterPro
IPR008948; L-Aspartase-like
; .
InterPro
IPR024083; L-Aspartase-like_N
; .
InterPro
IPR022761; Lyase1_N
; .
Gene3D
G3DSA:1.10.275.10; G3DSA:1.10.275.10
; 1; .
Pfam
PF10415; FumaraseC_C
; 1; .
Pfam
PF00206; Lyase_1
; 1; .
PRINTS
PR00149; FUMRATELYASE
; .
SUPFAM
SSF48557; L-Aspartase-like
; 1; .
TIGRFAMs
TIGR00979; FumC_II
; 1; .
PROSITE
PS00163; FUMARATE_LYASES
; 1; .
World-2DPAGE Repository (search AC)
Database constructed and maintained by
SIB
, using the
Make2D-DB II
package (
ver. 3.10.2
) from the
World-2DPAGE Constellation
of the
ExPASy web server