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Searching in 'World-2DPAGE Repository [0022]' for entry matching: P15651




World-2DPAGE Repository (0022):  P15651

P15651


General information about the entry
View entry in simple text format
Entry nameACADS_RAT
Primary accession numberP15651
integrated into World-2DPAGE Repository (0022) on May 7, 2010 (release 1)
2D Annotations were last modified onJune 22, 2011 (version 2)
General Annotations were last modified on November 24, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Short-chain specific acyl-CoA dehydrogenase, mitochondrial; Short=SCAD; EC=1.3.8.1; AltName: Full=Butyryl-CoA dehydrogenase; Flags: Precursor;.
Gene nameName=Acads
Annotated speciesRattus [TaxID: 10114]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000593;
Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L.
''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity''
Proteomics 11(16):3369-3379 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

HCR_LCR_HEART {HCR LCR Cardiac Map}
Rattus
Tissue: Heart
HCR_LCR_HEART
  map experimental info 		 
HCR_LCR_HEART

MAP LOCATIONS:
pI=8.47; Mw=45022  [identification data]
pI=8.47; Mw=45022  [identification data]
pI=8.47; Mw=45022  [identification data]
pI=8.47; Mw=45022  [identification data]

IDENTIFICATION: SPOT 4: SeqCov=30.6%. Peptides MS=10/20. Mascot PMF Score=69 [1]
SPOT 10: SeqCov=30.6%. Peptides MS=9/20. Mascot PMF Score=67 [1]; SPOT 11: SeqCov=28.2%. Peptides MS=9/20. Mascot PMF Score=65 [1]; SPOT 14: SeqCov=34.2%. Peptides MS=12/20. Mascot PMF Score=94 [1].
PEPTIDE EVIDENCE: SPOT 4: 387-ITEIYEGTSEIQR-399 (47) / 25-LHTVYQSVELPETHQMLR-42 (44) / 184-AWITNSWEASATVVFASTDR-203 (18) [1]
SPOT 10: 400-LVIAGHLLR-408 (50) / 314-LADMALALESAR-325 (80) / 235-ASSTANLIFEDCR-247 (61) [1]; SPOT 11: 400-LVIAGHLLR-408 (21) / 387-ITEIYEGTSEIQR-399 (64) / 184-AWITNSWEASATVVFASTDR-203 (97) [1]; SPOT 14: 400-LVIAGHLLR-408 (51) / 387-ITEIYEGTSEIQR-399 (71) / 184-AWITNSWEASATVVFASTDR-203 (51) [1].
MAPPING (identification):
SPOT 4: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 10: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 11: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 14: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright
Data from Dr. Jatin Burniston, Liverpool John Moores University, UK
Cross-references
UniProtKB/Swiss-ProtP15651; ACADS_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameACADS_RAT
Primary accession numberP15651
Sequence was last modified on November 1, 1995 (version 2)
Annotations were last modified on November 16, 2011 (version 117)
Name and origin of the protein
DescriptionRecName: Full=Short-chain specific acyl-CoA dehydrogenase, mitochondrial; Short=SCAD; EC=1.3.8.1; AltName: Full=Butyryl-CoA dehydrogenase; Flags: Precursor;
Gene nameName=Acads
Encoded onName=Acads
Keywords3D-structure; Complete proteome; Direct protein sequencing; FAD; Fatty acid metabolism; Flavoprotein; Lipid metabolism; Mitochondrion; Oxidoreductase; Reference proteome; Transit peptide.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLJ05030; AAA40669.1; ALT_INIT; mRNA
IPIIPI00231359; -; .
PIRB34252; B34252; .
RefSeqNP_071957.1; NM_022512.1; .
UniGeneRn.1167; -; .
PDB1JQI; X-ray; 2.25 A; A/B=25-412
PDBsum1JQI; -; .
ProteinModelPortalP15651; -; .
SMRP15651; 28-411; .
IntActP15651; 1; .
STRINGP15651; -; .
PRIDEP15651; -; .
GeneID64304; -; .
KEGGrno:64304; -; .
UCSCBC072545; rat; .
CTD35; -; .
RGD620514; Acads; .
eggNOGroNOG14644; -; .
HOVERGENHBG000224; -; .
InParanoidP15651; -; .
OrthoDBEOG4ZPDVH; -; .
PhylomeDBP15651; -; .
NextBio612942; -; .
ArrayExpressP15651; -; .
GenevestigatorP15651; -; .
GermOnlineENSRNOG00000001177; Rattus norvegicus; .
GOGO:0005759; C:mitochondrial matrix; IDA:BHF-UCL; .
GOGO:0031966; C:mitochondrial membrane; IDA:BHF-UCL; .
GOGO:0004085; F:butyryl-CoA dehydrogenase activity; IDA:RGD; .
GOGO:0000062; F:fatty-acyl-CoA binding; IDA:RGD; .
GOGO:0050660; F:flavin adenine dinucleotide binding; IDA:RGD; .
GOGO:0046359; P:butyrate catabolic process; IDA:RGD; .
GOGO:0033539; P:fatty acid beta-oxidation using acyl-CoA dehydrogenase; IDA:RGD; .
GOGO:0051289; P:protein homotetramerization; IDA:RGD; .
GOGO:0051384; P:response to glucocorticoid stimulus; IEP:RGD; .
GOGO:0042594; P:response to starvation; IEP:RGD; .
InterProIPR006089; Acyl-CoA_DH_CS; .
InterProIPR006092; Acyl-CoA_DH_N; .
InterProIPR006090; Acyl-CoA_Oxase/DH_1; .
InterProIPR006091; Acyl-CoA_Oxase/DH_cen-dom; .
InterProIPR009075; AcylCo_DH/oxidase_C; .
InterProIPR013786; AcylCoA_DH/ox_N; .
InterProIPR009100; AcylCoA_DH/oxidase; .
Gene3DG3DSA:2.40.110.10; Acyl_CoA_DH/ox_M; 1; .
Gene3DG3DSA:1.10.540.10; AcylCoA_DH/ox_N; 1; .
Gene3DG3DSA:1.20.140.10; AcylCoA_DH_1/2_C; 1; .
KOK00248; -; .
PfamPF00441; Acyl-CoA_dh_1; 1; .
PfamPF02770; Acyl-CoA_dh_M; 1; .
PfamPF02771; Acyl-CoA_dh_N; 1; .
SUPFAMSSF56645; AcylCoA_dehyd_NM; 1; .
SUPFAMSSF47203; AcylCoADH_C_like; 1; .
PROSITEPS00072; ACYL_COA_DH_1; 1; .
PROSITEPS00073; ACYL_COA_DH_2; 1; .


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World-2DPAGE Repository (search AC)

Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server