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World-2DPAGE Repository

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Searching in 'World-2DPAGE Repository [0030]' for entry matching: P16617

World-2DPAGE Repository (0030):  P16617


General information about the entry
View entry in simple text format
Entry namePGK1_RAT
Primary accession numberP16617
integrated into World-2DPAGE Repository (0030) on November 9, 2010 (release 1)
2D Annotations were last modified onJune 21, 2011 (version 2)
General Annotations were last modified on November 23, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Phosphoglycerate kinase 1; EC=;.
Gene nameName=Pgk1
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C.
''Two-dimensional gel proteome reference map of INS-1E cells''
Proteomics 11(7):1365-1369 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7}
Rattus norvegicus (Rat)
  map experimental info

pI=5.83; Mw=46113  [identification data]
pI=6.60; Mw=29212  [identification data]
pI=6.59; Mw=28180  [identification data]

IDENTIFICATION: SPOT 1489: Mascot protein score=124. Number of peptides=6 [1]
SPOT 2089: Mascot protein score=53. Number of peptides=3 [1]; SPOT 2139: Mascot protein score=109. Number of peptides=3 [1].
MAPPING (identification):
SPOT 1489: Tandem mass spectrometry [1];
SPOT 2089: Tandem mass spectrometry [1];
SPOT 2139: Tandem mass spectrometry [1].

Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium
UniProtKB/Swiss-ProtP16617; PGK1_RAT.
World-2DPAGE RepositoryP16617; PGK1_RAT.

2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry namePGK1_RAT
Primary accession numberP16617
Secondary accession number(s) Q5M945 Q6P508
Sequence was last modified on January 23, 2007 (version 2)
Annotations were last modified on November 16, 2011 (version 92)
Name and origin of the protein
DescriptionRecName: Full=Phosphoglycerate kinase 1; EC=;
Gene nameName=Pgk1
Encoded onName=Pgk1; Synonyms=Pgk-1
KeywordsAcetylation; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; Kinase; Nucleotide-binding; Phosphoprotein; Reference proteome; Transferase.
Copyrighted by the UniProt Consortium, see Distributed under the Creative Commons Attribution-NoDerivs License
EMBLM31788; AAA41838.1; -; mRNA
EMBLBC063161; AAH63161.1; -; mRNA
EMBLBC087651; AAH87651.1; -; mRNA
IPIIPI00231426; -; .
PIRA33792; A33792; .
RefSeqNP_445743.2; NM_053291.3; .
UniGeneRn.108127; -; .
ProteinModelPortalP16617; -; .
SMRP16617; 2-417; .
MINTMINT-4577521; -; .
STRINGP16617; -; .
PhosphoSiteP16617; -; .
World-2DPAGE0004:P16617; -; .
PRIDEP16617; -; .
EnsemblENSRNOT00000003390; ENSRNOP00000003390; ENSRNOG00000002467; .
GeneID24644; -; .
KEGGrno:24644; -; .
NMPDRfig|10116.3.peg.31805; -; .
UCSCNM_053291; rat; .
CTD5230; -; .
RGD619878; Pgk1; .
eggNOGroNOG09572; -; .
GeneTreeENSGT00390000008820; -; .
HOVERGENHBG008177; -; .
InParanoidP16617; -; .
OrthoDBEOG44MXS6; -; .
PhylomeDBP16617; -; .
NextBio603950; -; .
ArrayExpressP16617; -; .
GenevestigatorP16617; -; .
GermOnlineENSRNOG00000002467; Rattus norvegicus; .
GOGO:0005829; C:cytosol; IDA:RGD; .
GOGO:0005625; C:soluble fraction; IDA:RGD; .
GOGO:0043531; F:ADP binding; IDA:RGD; .
GOGO:0005524; F:ATP binding; ISS:UniProtKB; .
GOGO:0004618; F:phosphoglycerate kinase activity; ISS:UniProtKB; .
GOGO:0006094; P:gluconeogenesis; IDA:RGD; .
GOGO:0006096; P:glycolysis; IDA:RGD; .
InterProIPR001576; Phosphoglycerate_kinase; .
InterProIPR015901; Phosphoglycerate_kinase_C; .
InterProIPR015911; Phosphoglycerate_kinase_CS; .
InterProIPR015824; Phosphoglycerate_kinase_N; .
Gene3DG3DSA:; Phosphoglycerate_kinase_C; 1; .
Gene3DG3DSA:; Phosphoglycerate_kinase_N; 1; .
KOK00927; -; .
PfamPF00162; PGK; 1; .
PIRSFPIRSF000724; Pgk; 1; .
SUPFAMSSF53748; PGK; 1; .

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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server