World-2DPAGE Repository

RecName: Full=Hydroxymethylglutaryl-CoA synthase, cytoplasmic; Short=HMG-CoA synthase; EC=2.3.3.10; AltName: Full=3-hydroxy-3-methylglutaryl coenzyme A synthase;.

Gene name

Name=Hmgcs1
Synonyms=Hmgcs

Annotated species

Rattus norvegicus (Rat) [TaxID: 10116]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	2D-PAGE GEL CHARACTERIZATION DOI=10.1002/pmic.200900680; Maurya D.K., Sundaram C.S., Bhargava P. ''Proteome profile of whole cerebellum of the mature rat'' Proteomics 10(23):4311-4319 (2010)

2D PAGE maps for identified proteins

How to interpret a protein

RAT_CEREBELLUM_21D {Proteome of 21 days old rat cerebellum}
Rattus norvegicus (Rat)
Tissue: Cerebellum

map experimental info

RAT_CEREBELLUM_21D

MAP LOCATIONS:

SPOT 2850:

pI=5.85; Mw=57000

[identification data]

IDENTIFICATION: SPOT 2850: SeqCov=28%. MascotPMF=71 [1].
MAPPING (identification):

SPOT 2850: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Purnima Bhargava, Centre for Cellular and Molecular Biology, India

Cross-references

PRIDE

10017.

UniProtKB/Swiss-Prot

P17425; HMCS1_RAT.

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry name	HMCS1_RAT
Primary accession number	P17425
Sequence was last modified on	August 1, 1990 (version 1)
Annotations were last modified on	November 16, 2011 (version 104)
Name and origin of the protein
Description	RecName: Full=Hydroxymethylglutaryl-CoA synthase, cytoplasmic; Short=HMG-CoA synthase; EC=2.3.3.10; AltName: Full=3-hydroxy-3-methylglutaryl coenzyme A synthase;
Gene name	Name=Hmgcs1 Synonyms=Hmgcs
Encoded on	Name=Hmgcs1; Synonyms=Hmgcs
Keywords	Acetylation; Cholesterol biosynthesis; Complete proteome; Cytoplasm; Direct protein sequencing; Lipid synthesis; Phosphoprotein; Reference proteome; Steroid biosynthesis; Sterol biosynthesis; Transferase.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	X52625; CAA36852.1; -; mRNA
IPI	IPI00188158; -; .
PIR	S12736; S12736; .
RefSeq	NP_058964.1; NM_017268.1; .
UniGene	Rn.5106; -; .
ProteinModelPortal	P17425; -; .
SMR	P17425; 16-470; .
STRING	P17425; -; .
PhosphoSite	P17425; -; .
PRIDE	P17425; -; .
Ensembl	ENSRNOT00000023554; ENSRNOP00000023554; ENSRNOG00000016552; .
GeneID	29637; -; .
KEGG	rno:29637; -; .
NMPDR	fig\|10116.3.peg.15558; -; .
UCSC	NM_017268; rat; .
CTD	3157; -; .
RGD	70970; Hmgcs1; .
eggNOG	roNOG08859; -; .
GeneTree	ENSGT00390000006096; -; .
HOVERGEN	HBG051912; -; .
InParanoid	P17425; -; .
OMA	DTVNACY; -; .
OrthoDB	EOG4F1X30; -; .
PhylomeDB	P17425; -; .
BRENDA	2.3.3.10; 5301; .
NextBio	609884; -; .
ArrayExpress	P17425; -; .
Genevestigator	P17425; -; .
GermOnline	ENSRNOG00000016552; Rattus norvegicus; .
GO	GO:0005829; C:cytosol; IDA:RGD; .
GO	GO:0008144; F:drug binding; IDA:RGD; .
GO	GO:0004421; F:hydroxymethylglutaryl-CoA synthase activity; IDA:RGD; .
GO	GO:0016853; F:isomerase activity; IDA:RGD; .
GO	GO:0043177; F:organic acid binding; IDA:RGD; .
GO	GO:0042803; F:protein homodimerization activity; IDA:RGD; .
GO	GO:0007420; P:brain development; IEP:RGD; .
GO	GO:0071397; P:cellular response to cholesterol; IEP:RGD; .
GO	GO:0071372; P:cellular response to follicle-stimulating hormone stimulus; IEP:RGD; .
GO	GO:0071407; P:cellular response to organic cyclic compound; IEP:RGD; .
GO	GO:0006695; P:cholesterol biosynthetic process; IEA:UniProtKB-KW; .
GO	GO:0008299; P:isoprenoid biosynthetic process; IEA:InterPro; .
GO	GO:0001889; P:liver development; IEP:RGD; .
GO	GO:0008584; P:male gonad development; IEP:RGD; .
GO	GO:0001101; P:response to acid; IEP:RGD; .
GO	GO:0042493; P:response to drug; IEP:RGD; .
GO	GO:0055094; P:response to lipoprotein stimulus; IEP:RGD; .
GO	GO:0009645; P:response to low light intensity stimulus; IEP:RGD; .
GO	GO:0010243; P:response to organic nitrogen; IDA:RGD; .
GO	GO:0014074; P:response to purine-containing compound; IEP:RGD; .
GO	GO:0046690; P:response to tellurium ion; IEP:RGD; .
GO	GO:0033197; P:response to vitamin E; IEP:RGD; .
InterPro	IPR000590; HMG_CoA_synt_AS; .
InterPro	IPR013746; HMG_CoA_synt_C; .
InterPro	IPR013528; HMG_CoA_synth_N; .
InterPro	IPR010122; HMG_CoA_synthase_euk; .
InterPro	IPR016039; Thiolase-like; .
InterPro	IPR016038; Thiolase-like_subgr; .
Gene3D	G3DSA:3.40.47.10; Thiolase-like_subgr; 1; .
KO	K01641; -; .
Pfam	PF08540; HMG_CoA_synt_C; 1; .
Pfam	PF01154; HMG_CoA_synt_N; 1; .
SUPFAM	SSF53901; Thiolase-like; 2; .
TIGRFAMs	TIGR01833; HMG-CoA-S_euk; 1; .
PROSITE	PS01226; HMG_COA_SYNTHASE; 1; .

World-2DPAGE Repository (search AC)

Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server

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