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Searching in 'World-2DPAGE Repository [0030]' for entry matching: P27605




World-2DPAGE Repository (0030):  P27605


P27605


General information about the entry
View entry in simple text format
Entry nameHPRT_RAT
Primary accession numberP27605
integrated into World-2DPAGE Repository (0030) on November 9, 2010 (release 1)
2D Annotations were last modified onJune 21, 2011 (version 2)
General Annotations were last modified on November 23, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Hypoxanthine-guanine phosphoribosyltransferase; Short=HGPRT; Short=HGPRTase; EC=2.4.2.8;.
Gene nameName=Hprt1
Synonyms=Hprt
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000006;
D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C.
''Two-dimensional gel proteome reference map of INS-1E cells''
Proteomics 11(7):1365-1369 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7}
Rattus norvegicus (Rat)
RAT_INS1E_4-7
  map experimental info
 
RAT_INS1E_4-7

MAP LOCATIONS:
pI=6.33; Mw=29742  [identification data]
pI=6.32; Mw=28810  [identification data]

IDENTIFICATION: SPOT 2079: Mascot protein score=69. Number of peptides=4 [1]
SPOT 2106: Mascot protein score=50. Number of peptides=3 [1].
MAPPING (identification):
SPOT 2079: Tandem mass spectrometry [1];
SPOT 2106: Tandem mass spectrometry [1].

Copyright
Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium
Cross-references
UniProtKB/Swiss-ProtP27605; HPRT_RAT.
World-2DPAGE RepositoryP27605; HPRT_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameHPRT_RAT
Primary accession numberP27605
Secondary accession number(s) P70469 Q4KMC5 Q62926
Sequence was last modified on August 1, 1992 (version 1)
Annotations were last modified on November 16, 2011 (version 93)
Name and origin of the protein
DescriptionRecName: Full=Hypoxanthine-guanine phosphoribosyltransferase; Short=HGPRT; Short=HGPRTase; EC=2.4.2.8;
Gene nameName=Hprt1
Synonyms=Hprt
Encoded onName=Hprt1; Synonyms=Hprt
KeywordsComplete proteome; Cytoplasm; Direct protein sequencing; Glycosyltransferase; Magnesium; Metal-binding; Nucleotide-binding; Purine salvage; Reference proteome; Transferase.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM63983; AAA41350.1; -; mRNA
EMBLS79292; AAB21288.1; -; mRNA
EMBLX62085; CAA43997.1; -; mRNA
EMBLAF001282; AAB65640.1; -; Genomic_DNA
EMBLAF001278; AAB65640.1; JOINED; Genomic_DNA
EMBLAF009655; AAB65640.1; JOINED; Genomic_DNA
EMBLAF009656; AAB65640.1; JOINED; Genomic_DNA
EMBLAF001279; AAB65640.1; JOINED; Genomic_DNA
EMBLAF001280; AAB65640.1; JOINED; Genomic_DNA
EMBLAF001281; AAB65640.1; JOINED; Genomic_DNA
EMBLBC098629; AAH98629.1; -; mRNA
EMBLU06049; AAA56887.1; -; Genomic_DNA
IPIIPI00560533; -; .
PIRS18140; S18140; .
RefSeqNP_036715.1; NM_012583.2; .
UniGeneRn.47; -; .
ProteinModelPortalP27605; -; .
SMRP27605; 5-218; .
IntActP27605; 2; .
STRINGP27605; -; .
PhosphoSiteP27605; -; .
World-2DPAGE0004:P27605; -; .
PRIDEP27605; -; .
EnsemblENSRNOT00000045153; ENSRNOP00000043388; ENSRNOG00000031367; .
GeneID24465; -; .
KEGGrno:24465; -; .
UCSCNM_012583; rat; .
CTD3251; -; .
RGD2826; Hprt1; .
eggNOGroNOG08855; -; .
HOVERGENHBG000242; -; .
InParanoidP27605; -; .
OrthoDBEOG4H72CM; -; .
PhylomeDBP27605; -; .
NextBio603395; -; .
ArrayExpressP27605; -; .
GenevestigatorP27605; -; .
GermOnlineENSRNOG00000031367; Rattus norvegicus; .
GOGO:0005829; C:cytosol; IBA:RefGenome; .
GOGO:0005625; C:soluble fraction; IDA:RGD; .
GOGO:0052657; F:guanine phosphoribosyltransferase activity; ISS:UniProtKB; .
GOGO:0004422; F:hypoxanthine phosphoribosyltransferase activity; IDA:RGD; .
GOGO:0000287; F:magnesium ion binding; IBA:RefGenome; .
GOGO:0000166; F:nucleotide binding; IEA:UniProtKB-KW; .
GOGO:0006168; P:adenine salvage; IBA:RefGenome; .
GOGO:0032869; P:cellular response to insulin stimulus; IEP:RGD; .
GOGO:0021954; P:central nervous system neuron development; IBA:RefGenome; .
GOGO:0021895; P:cerebral cortex neuron differentiation; IBA:RefGenome; .
GOGO:0019835; P:cytolysis; IBA:RefGenome; .
GOGO:0048813; P:dendrite morphogenesis; IBA:RefGenome; .
GOGO:0046038; P:GMP catabolic process; ISS:UniProtKB; .
GOGO:0032263; P:GMP salvage; IBA:RefGenome; .
GOGO:0007625; P:grooming behavior; IBA:RefGenome; .
GOGO:0006178; P:guanine salvage; ISS:UniProtKB; .
GOGO:0043103; P:hypoxanthine salvage; ISS:UniProtKB; .
GOGO:0032264; P:IMP salvage; IBA:RefGenome; .
GOGO:0046651; P:lymphocyte proliferation; IBA:RefGenome; .
GOGO:0045964; P:positive regulation of dopamine metabolic process; IBA:RefGenome; .
GOGO:0006166; P:purine ribonucleoside salvage; IBA:RefGenome; .
GOGO:0001975; P:response to amphetamine; IBA:RefGenome; .
GOGO:0007283; P:spermatogenesis; IEP:RGD; .
GOGO:0021756; P:striatum development; IBA:RefGenome; .
InterProIPR005904; Hxn_phspho_trans; .
InterProIPR000836; PRibTrfase; .
KOK00760; -; .
PfamPF00156; Pribosyltran; 1; .
TIGRFAMsTIGR01203; HGPRTase; 1; .
PROSITEPS00103; PUR_PYR_PR_TRANSFER; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server