World-2DPAGE Repository

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	MAPPING ON GEL PubMed=19343716; DOI=10.1002/pmic.200800664; [NCBI, EBI, Israel, Japan] Maurya D.K., Sundaram C.S., Bhargava P. ''Proteome profile of the mature rat olfactory bulb'' Proteomics 9(1):2593-2599 (2009)

2D PAGE maps for identified proteins

How to interpret a protein

OB21DSUB {2-DE gel for Olfactory bulb proteome}
Rattus norvegicus (Rat)
Tissue: Olfactory bulb

map experimental info

OB21DSUB

MAP LOCATIONS:

SPOT 203:

pI=5.87; Mw=75000

[identification data]

MAPPING (identification):

SPOT 203: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Purnima Bhargava, Centre for Cellular and Molecular Biology, India

Cross-references

UniProtKB/Swiss-Prot

P35571; GPDM_RAT.

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry name	GPDM_RAT
Primary accession number	P35571
Sequence was last modified on	June 1, 1994 (version 1)
Annotations were last modified on	November 16, 2011 (version 99)
Name and origin of the protein
Description	RecName: Full=Glycerol-3-phosphate dehydrogenase, mitochondrial; Short=GPD-M; Short=GPDH-M; EC=1.1.5.3; Flags: Precursor;
Gene name	Name=Gpd2
Encoded on	Name=Gpd2
Keywords	Calcium; Complete proteome; Direct protein sequencing; FAD; Flavoprotein; Mitochondrion; Oxidoreductase; Phosphoprotein; Reference proteome; Repeat; Transit peptide.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	U08027; AAB60443.1; -; mRNA
EMBL	X78593; CAA55329.1; -; mRNA
EMBL	BC083565; AAH83565.1; -; mRNA
IPI	IPI00199663; -; .
PIR	A54051; A54051; .
RefSeq	NP_036868.1; NM_012736.1; .
UniGene	Rn.89705; -; .
ProteinModelPortal	P35571; -; .
STRING	P35571; -; .
PhosphoSite	P35571; -; .
World-2DPAGE	0004:P35571; -; .
PRIDE	P35571; -; .
GeneID	25062; -; .
KEGG	rno:25062; -; .
CTD	2820; -; .
RGD	2726; Gpd2; .
eggNOG	roNOG06508; -; .
HOVERGEN	HBG005897; -; .
PhylomeDB	P35571; -; .
NextBio	605290; -; .
Genevestigator	P35571; -; .
GO	GO:0009331; C:glycerol-3-phosphate dehydrogenase complex; IEA:InterPro; .
GO	GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell; .
GO	GO:0005509; F:calcium ion binding; IEA:InterPro; .
GO	GO:0004367; F:glycerol-3-phosphate dehydrogenase [NAD+] activity; IDA:RGD; .
GO	GO:0052591; F:sn-glycerol-3-phosphate:ubiquinone-8 oxidoreductase activity; IEA:EC; .
GO	GO:0006072; P:glycerol-3-phosphate metabolic process; IDA:RGD; .
GO	GO:0006734; P:NADH metabolic process; IDA:RGD; .
InterPro	IPR011992; EF-hand-like_dom; .
InterPro	IPR018247; EF_Hand_1_Ca_BS; .
InterPro	IPR018249; EF_HAND_2; .
InterPro	IPR002048; EF_hand_Ca-bd; .
InterPro	IPR006076; FAD-dep_OxRdtase; .
InterPro	IPR000447; G3P_DH_FAD-dep; .
Gene3D	G3DSA:1.10.238.10; EF-Hand_type; 1; .
KO	K00111; -; .
Pfam	PF01266; DAO; 1; .
PRINTS	PR01001; FADG3PDH; .
SMART	SM00054; EFh; 2; .
PROSITE	PS00018; EF_HAND_1; 1; .
PROSITE	PS50222; EF_HAND_2; 2; .
PROSITE	PS00977; FAD_G3PDH_1; 1; .
PROSITE	PS00978; FAD_G3PDH_2; 1; .

World-2DPAGE Repository (search AC)

Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server

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