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Searching in 'World-2DPAGE Repository [0030]' for entry matching: P41562




World-2DPAGE Repository (0030):  P41562


P41562


General information about the entry
View entry in simple text format
Entry nameIDHC_RAT
Primary accession numberP41562
integrated into World-2DPAGE Repository (0030) on November 9, 2010 (release 1)
2D Annotations were last modified onJune 21, 2011 (version 2)
General Annotations were last modified on November 23, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Isocitrate dehydrogenase [NADP] cytoplasmic; Short=IDH; EC=1.1.1.42; AltName: Full=Cytosolic NADP-isocitrate dehydrogenase; AltName: Full=IDP; AltName: Full=NADP(+)-specific ICDH; AltName: Full=Oxalosuccinate decarboxylase;.
Gene nameName=Idh1
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000006;
D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C.
''Two-dimensional gel proteome reference map of INS-1E cells''
Proteomics 11(7):1365-1369 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7}
Rattus norvegicus (Rat)
RAT_INS1E_4-7
  map experimental info
 
RAT_INS1E_4-7

MAP LOCATIONS:
pI=6.71; Mw=53396  [identification data]

IDENTIFICATION: SPOT 1286: Mascot protein score=180. Number of peptides=5 [1].
MAPPING (identification):
SPOT 1286: Tandem mass spectrometry [1].

Copyright
Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium
Cross-references
UniProtKB/Swiss-ProtP41562; IDHC_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameIDHC_RAT
Primary accession numberP41562
Secondary accession number(s) P80300
Sequence was last modified on November 1, 1995 (version 1)
Annotations were last modified on November 16, 2011 (version 88)
Name and origin of the protein
DescriptionRecName: Full=Isocitrate dehydrogenase [NADP] cytoplasmic; Short=IDH; EC=1.1.1.42; AltName: Full=Cytosolic NADP-isocitrate dehydrogenase; AltName: Full=IDP; AltName: Full=NADP(+)-specific ICDH; AltName: Full=Oxalosuccinate decarboxylase;
Gene nameName=Idh1
Encoded onName=Idh1
KeywordsAcetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Glyoxylate bypass; Magnesium; Manganese; Metal-binding; NADP; Oxidoreductase; Reference proteome; Tricarboxylic acid cycle.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLL35317; AAA59356.1; -; mRNA
IPIIPI00194045; -; .
PIRA54756; A54756; .
RefSeqNP_113698.1; NM_031510.1; .
UniGeneRn.3561; -; .
ProteinModelPortalP41562; -; .
SMRP41562; 4-413; .
STRINGP41562; -; .
PhosphoSiteP41562; -; .
PRIDEP41562; -; .
EnsemblENSRNOT00000020322; ENSRNOP00000020322; ENSRNOG00000015020; .
GeneID24479; -; .
KEGGrno:24479; -; .
UCSCNM_031510; rat; .
CTD3417; -; .
RGD2862; Idh1; .
eggNOGroNOG04762; -; .
HOVERGENHBG006119; -; .
OrthoDBEOG47M1Z0; -; .
PhylomeDBP41562; -; .
BRENDA1.1.1.42; 5301; .
NextBio603439; -; .
ArrayExpressP41562; -; .
GenevestigatorP41562; -; .
GermOnlineENSRNOG00000015020; Rattus norvegicus; .
GOGO:0005777; C:peroxisome; IDA:HGNC; .
GOGO:0005625; C:soluble fraction; IDA:RGD; .
GOGO:0004450; F:isocitrate dehydrogenase (NADP+) activity; ISS:UniProtKB; .
GOGO:0000287; F:magnesium ion binding; ISS:UniProtKB; .
GOGO:0051287; F:NAD binding; IEA:InterPro; .
GOGO:0050661; F:NADP binding; IDA:RGD; .
GOGO:0006103; P:2-oxoglutarate metabolic process; ISS:UniProtKB; .
GOGO:0008585; P:female gonad development; IEP:RGD; .
GOGO:0006097; P:glyoxylate cycle; IEA:UniProtKB-KW; .
GOGO:0006102; P:isocitrate metabolic process; ISS:UniProtKB; .
GOGO:0014070; P:response to organic cyclic compound; IDA:RGD; .
GOGO:0048545; P:response to steroid hormone stimulus; IDA:RGD; .
GOGO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW; .
InterProIPR019818; IsoCit/isopropylmalate_DH_CS; .
InterProIPR004790; Isocitrate_DH_NADP_euk-typ; .
InterProIPR024084; IsoPropMal-DH-like_dom; .
Gene3DG3DSA:3.40.718.10; IDH_IMDH; 1; .
KOK00031; -; .
PANTHERPTHR11822; IDH_NADP_euk; 1; .
PfamPF00180; Iso_dh; 1; .
PIRSFPIRSF000108; IDH_NADP; 1; .
TIGRFAMsTIGR00127; Nadp_idh_euk; 1; .
PROSITEPS00470; IDH_IMDH; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server