Attention: World-2DPAGE is no longer maintained. Previously submitted data can still be queried at World-2DPAGE Repository.

OB21DSUB {2-DE gel for Olfactory bulb proteome}
Rattus norvegicus (Rat)
Tissue: Olfactory bulb

OB21DSUB

MAP LOCATIONS:

SPOT 208:

pI=5.97; Mw=73000

MAPPING (identification):

SPOT 208: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Purnima Bhargava, Centre for Cellular and Molecular Biology, India

Cross-references

UniProtKB/Swiss-Prot

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry name	GRP75_RAT
Primary accession number	P48721
Sequence was last modified on	October 17, 2006 (version 3)
Annotations were last modified on	November 16, 2011 (version 95)
Name and origin of the protein
Description	RecName: Full=Stress-70 protein, mitochondrial; AltName: Full=75 kDa glucose-regulated protein; Short=GRP-75; AltName: Full=Heat shock 70 kDa protein 9; AltName: Full=Mortalin; AltName: Full=Peptide-binding protein 74; Short=PBP74; AltName: Full=mtHSP70; Flags: Precursor;
Gene name	Name=Hspa9 Synonyms=Grp75, Hspa9a
Encoded on	Name=Hspa9; Synonyms=Grp75, Hspa9a
Keywords	Acetylation; ATP-binding; Chaperone; Complete proteome; Direct protein sequencing; Mitochondrion; Nucleotide-binding; Phosphoprotein; Reference proteome; Transit peptide.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	S75280; AAB33049.1; -; mRNA
EMBL	S78556; AAB34982.1; -; mRNA
IPI	IPI00363265; -; .
PIR	I56581; I56581; .
RefSeq	NP_001094128.1; NM_001100658.1; .
UniGene	Rn.7535; -; .
ProteinModelPortal	P48721; -; .
SMR	P48721; 55-580; .
IntAct	P48721; 2; .
MINT	MINT-4592308; -; .
STRING	P48721; -; .
PhosphoSite	P48721; -; .
World-2DPAGE	0004:P48721; -; .
PRIDE	P48721; -; .
GeneID	291671; -; .
KEGG	rno:291671; -; .
UCSC	S78556; rat; .
CTD	3313; -; .
RGD	1311806; Hspa9; .
eggNOG	maNOG05443; -; .
GeneTree	ENSGT00550000074813; -; .
HOVERGEN	HBG051845; -; .
InParanoid	P48721; -; .
OrthoDB	EOG49KFQ6; -; .
PhylomeDB	P48721; -; .
NextBio	632983; -; .
ArrayExpress	P48721; -; .
Genevestigator	P48721; -; .
GermOnline	ENSRNOG00000019525; Rattus norvegicus; .
GO	GO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GO	GO:0051082; F:unfolded protein binding; IEA:InterPro; .
GO	GO:0006457; P:protein folding; IEA:InterPro; .
InterPro	IPR012725; Chaperone_DnaK; .
InterPro	IPR018181; Heat_shock_70_CS; .
InterPro	IPR001023; Hsp70; .
InterPro	IPR013126; Hsp_70; .
KO	K04043; -; .
PANTHER	PTHR19375; Hsp70; 1; .
Pfam	PF00012; HSP70; 1; .
PRINTS	PR00301; HEATSHOCK70; .
TIGRFAMs	TIGR02350; Prok_dnaK; 1; .
PROSITE	PS00297; HSP70_1; 1; .
PROSITE	PS00329; HSP70_2; 1; .
PROSITE	PS01036; HSP70_3; 1; .

Searching in 'World-2DPAGE Repository [0014]' for entry matching: P48721

World-2DPAGE Repository (0014): P48721

P48721

General information about the entry

Entry name

GRP75_RAT

Primary accession number

P48721

integrated into World-2DPAGE Repository (0014) on

October 13, 2008 (release 1)

2D Annotations were last modified on

June 1, 2011 (version 3)

General Annotations were last modified on

November 25, 2011 (version 2)

Name and origin of the protein

Description

Gene name

Name=Hspa9
Synonyms=Grp75, Hspa9a

Annotated species

Rattus norvegicus (Rat) [TaxID: 10116]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	2D-PAGE GEL CHARACTERIZATION DOI=10.1002/pmic.200900680; Maurya D.K., Sundaram C.S., Bhargava P. ''Proteome profile of whole cerebellum of the mature rat'' Proteomics 10(23):4311-4319 (2010)

2D PAGE maps for identified proteins

RAT_CEREBELLUM_21D {Proteome of 21 days old rat cerebellum}
Rattus norvegicus (Rat)
Tissue: Cerebellum

RAT_CEREBELLUM_21D

MAP LOCATIONS:

SPOT 2765:

pI=5.64; Mw=76000

IDENTIFICATION: SPOT 2765: SeqCov=27%. MascotPMF=101 [1].
MAPPING (identification):

SPOT 2765: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Purnima Bhargava, Centre for Cellular and Molecular Biology, India

Cross-references

PRIDE

10017.

UniProtKB/Swiss-Prot

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry name	GRP75_RAT
Primary accession number	P48721
Sequence was last modified on	October 17, 2006 (version 3)
Annotations were last modified on	November 16, 2011 (version 95)
Name and origin of the protein
Description	RecName: Full=Stress-70 protein, mitochondrial; AltName: Full=75 kDa glucose-regulated protein; Short=GRP-75; AltName: Full=Heat shock 70 kDa protein 9; AltName: Full=Mortalin; AltName: Full=Peptide-binding protein 74; Short=PBP74; AltName: Full=mtHSP70; Flags: Precursor;
Gene name	Name=Hspa9 Synonyms=Grp75, Hspa9a
Encoded on	Name=Hspa9; Synonyms=Grp75, Hspa9a
Keywords	Acetylation; ATP-binding; Chaperone; Complete proteome; Direct protein sequencing; Mitochondrion; Nucleotide-binding; Phosphoprotein; Reference proteome; Transit peptide.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	S75280; AAB33049.1; -; mRNA
EMBL	S78556; AAB34982.1; -; mRNA
IPI	IPI00363265; -; .
PIR	I56581; I56581; .
RefSeq	NP_001094128.1; NM_001100658.1; .
UniGene	Rn.7535; -; .
ProteinModelPortal	P48721; -; .
SMR	P48721; 55-580; .
IntAct	P48721; 2; .
MINT	MINT-4592308; -; .
STRING	P48721; -; .
PhosphoSite	P48721; -; .
World-2DPAGE	0004:P48721; -; .
PRIDE	P48721; -; .
GeneID	291671; -; .
KEGG	rno:291671; -; .
UCSC	S78556; rat; .
CTD	3313; -; .
RGD	1311806; Hspa9; .
eggNOG	maNOG05443; -; .
GeneTree	ENSGT00550000074813; -; .
HOVERGEN	HBG051845; -; .
InParanoid	P48721; -; .
OrthoDB	EOG49KFQ6; -; .
PhylomeDB	P48721; -; .
NextBio	632983; -; .
ArrayExpress	P48721; -; .
Genevestigator	P48721; -; .
GermOnline	ENSRNOG00000019525; Rattus norvegicus; .
GO	GO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GO	GO:0051082; F:unfolded protein binding; IEA:InterPro; .
GO	GO:0006457; P:protein folding; IEA:InterPro; .
InterPro	IPR012725; Chaperone_DnaK; .
InterPro	IPR018181; Heat_shock_70_CS; .
InterPro	IPR001023; Hsp70; .
InterPro	IPR013126; Hsp_70; .
KO	K04043; -; .
PANTHER	PTHR19375; Hsp70; 1; .
Pfam	PF00012; HSP70; 1; .
PRINTS	PR00301; HEATSHOCK70; .
TIGRFAMs	TIGR02350; Prok_dnaK; 1; .
PROSITE	PS00297; HSP70_1; 1; .
PROSITE	PS00329; HSP70_2; 1; .
PROSITE	PS01036; HSP70_3; 1; .

Searching in 'World-2DPAGE Repository [0019]' for entry matching: P48721

World-2DPAGE Repository (0019): P48721

P48721

General information about the entry

Entry name

GRP75_RAT

Primary accession number

P48721

integrated into World-2DPAGE Repository (0019) on

December 9, 2009 (release 1)

2D Annotations were last modified on

May 30, 2011 (version 2)

General Annotations were last modified on

January 13, 2012 (version 2)

Name and origin of the protein

Description

Gene name

Name=Hspa9
Synonyms=Grp75, Hspa9a

Annotated species

Rattus norvegicus (Rat) [TaxID: 10116]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	2D-PAGE GEL CHARACTERIZATION DOI=10.1111/j.1471-4159.2010.06719.x; VanGuilder H.D., Yan H., Farley J.A., Sonntag W.E., Freeman W.M. ''Aging alters the expression of neurotransmission-regulating proteins in the hippocampal synaptoproteome'' J of Neurochemistry 113(6):1577-1588 (2010)

2D PAGE maps for identified proteins

HIPP_SYNAP {Rat hippocampal synaptoproteome}
Rattus norvegicus (Rat)
Tissue: Hippocampus

HIPP_SYNAP

MAP LOCATIONS:

SPOT 280:

pI=5.87; Mw=73983

SPOT 283:

pI=5.97; Mw=74098

IDENTIFICATION: SPOT 280: Peptides number=14. SeqCov=25.2%. MOWSE score=1.22E+11 [1]
SPOT 283: Peptides number=8. SeqCov=14.1%. MOWSE score=6.78E+8 [1].
MAPPING (identification):

SPOT 280: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 283: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Willard Freeman, Penn State College of Medicine, USA

Cross-references

UniProtKB/Swiss-Prot

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry name	GRP75_RAT
Primary accession number	P48721
Sequence was last modified on	October 17, 2006 (version 3)
Annotations were last modified on	November 16, 2011 (version 95)
Name and origin of the protein
Description	RecName: Full=Stress-70 protein, mitochondrial; AltName: Full=75 kDa glucose-regulated protein; Short=GRP-75; AltName: Full=Heat shock 70 kDa protein 9; AltName: Full=Mortalin; AltName: Full=Peptide-binding protein 74; Short=PBP74; AltName: Full=mtHSP70; Flags: Precursor;
Gene name	Name=Hspa9 Synonyms=Grp75, Hspa9a
Encoded on	Name=Hspa9; Synonyms=Grp75, Hspa9a
Keywords	Acetylation; ATP-binding; Chaperone; Complete proteome; Direct protein sequencing; Mitochondrion; Nucleotide-binding; Phosphoprotein; Reference proteome; Transit peptide.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	S75280; AAB33049.1; -; mRNA
EMBL	S78556; AAB34982.1; -; mRNA
IPI	IPI00363265; -; .
PIR	I56581; I56581; .
RefSeq	NP_001094128.1; NM_001100658.1; .
UniGene	Rn.7535; -; .
ProteinModelPortal	P48721; -; .
SMR	P48721; 55-580; .
IntAct	P48721; 2; .
MINT	MINT-4592308; -; .
STRING	P48721; -; .
PhosphoSite	P48721; -; .
World-2DPAGE	0004:P48721; -; .
PRIDE	P48721; -; .
GeneID	291671; -; .
KEGG	rno:291671; -; .
UCSC	S78556; rat; .
CTD	3313; -; .
RGD	1311806; Hspa9; .
eggNOG	maNOG05443; -; .
GeneTree	ENSGT00550000074813; -; .
HOVERGEN	HBG051845; -; .
InParanoid	P48721; -; .
OrthoDB	EOG49KFQ6; -; .
PhylomeDB	P48721; -; .
NextBio	632983; -; .
ArrayExpress	P48721; -; .
Genevestigator	P48721; -; .
GermOnline	ENSRNOG00000019525; Rattus norvegicus; .
GO	GO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GO	GO:0051082; F:unfolded protein binding; IEA:InterPro; .
GO	GO:0006457; P:protein folding; IEA:InterPro; .
InterPro	IPR012725; Chaperone_DnaK; .
InterPro	IPR018181; Heat_shock_70_CS; .
InterPro	IPR001023; Hsp70; .
InterPro	IPR013126; Hsp_70; .
KO	K04043; -; .
PANTHER	PTHR19375; Hsp70; 1; .
Pfam	PF00012; HSP70; 1; .
PRINTS	PR00301; HEATSHOCK70; .
TIGRFAMs	TIGR02350; Prok_dnaK; 1; .
PROSITE	PS00297; HSP70_1; 1; .
PROSITE	PS00329; HSP70_2; 1; .
PROSITE	PS01036; HSP70_3; 1; .

Searching in 'World-2DPAGE Repository [0022]' for entry matching: P48721

World-2DPAGE Repository (0022): P48721

P48721

General information about the entry

Entry name

GRP75_RAT

Primary accession number

P48721

integrated into World-2DPAGE Repository (0022) on

May 7, 2010 (release 1)

2D Annotations were last modified on

June 22, 2011 (version 2)

General Annotations were last modified on

November 24, 2011 (version 2)

Name and origin of the protein

Description

Gene name

Name=Hspa9
Synonyms=Grp75, Hspa9a

Annotated species

Rattus [TaxID: 10114]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae.

References

[1]

2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000593;
Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L.
''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity''
Proteomics 11(16):3369-3379 (2011)

2D PAGE maps for identified proteins

HCR_LCR_HEART {HCR LCR Cardiac Map}
Rattus
Tissue: Heart

HCR_LCR_HEART

MAP LOCATIONS:

SPOT 785:

pI=5.97; Mw=74097

SPOT 790:

pI=5.97; Mw=74097

IDENTIFICATION: SPOT 785: SeqCov=20%. Peptides MS=12/20. Mascot PMF Score=84 [1]
SPOT 790: SeqCov=11.9%. Peptides MS=7/20. Mascot PMF Score=33 [1].
PEPTIDE EVIDENCE: SPOT 785: 378-SDIGEVILVGGMTR-391 (104) / 378-SDIGEVILVGGMTR-391 Oxidation (M) (48) / 499-LLGQFTLIGIPPAPR-513 (70) [1]
SPOT 790: 378-SDIGEVILVGGMTR-391 (81) / 378-SDIGEVILVGGMTR-391 Oxidation (M) (37) / 499-LLGQFTLIGIPPAPR-513 (57) [1].
MAPPING (identification):

SPOT 785: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 790: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Jatin Burniston, Liverpool John Moores University, UK

Cross-references

UniProtKB/Swiss-Prot

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry name	GRP75_RAT
Primary accession number	P48721
Sequence was last modified on	October 17, 2006 (version 3)
Annotations were last modified on	November 16, 2011 (version 95)
Name and origin of the protein
Description	RecName: Full=Stress-70 protein, mitochondrial; AltName: Full=75 kDa glucose-regulated protein; Short=GRP-75; AltName: Full=Heat shock 70 kDa protein 9; AltName: Full=Mortalin; AltName: Full=Peptide-binding protein 74; Short=PBP74; AltName: Full=mtHSP70; Flags: Precursor;
Gene name	Name=Hspa9 Synonyms=Grp75, Hspa9a
Encoded on	Name=Hspa9; Synonyms=Grp75, Hspa9a
Keywords	Acetylation; ATP-binding; Chaperone; Complete proteome; Direct protein sequencing; Mitochondrion; Nucleotide-binding; Phosphoprotein; Reference proteome; Transit peptide.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	S75280; AAB33049.1; -; mRNA
EMBL	S78556; AAB34982.1; -; mRNA
IPI	IPI00363265; -; .
PIR	I56581; I56581; .
RefSeq	NP_001094128.1; NM_001100658.1; .
UniGene	Rn.7535; -; .
ProteinModelPortal	P48721; -; .
SMR	P48721; 55-580; .
IntAct	P48721; 2; .
MINT	MINT-4592308; -; .
STRING	P48721; -; .
PhosphoSite	P48721; -; .
World-2DPAGE	0004:P48721; -; .
PRIDE	P48721; -; .
GeneID	291671; -; .
KEGG	rno:291671; -; .
UCSC	S78556; rat; .
CTD	3313; -; .
RGD	1311806; Hspa9; .
eggNOG	maNOG05443; -; .
GeneTree	ENSGT00550000074813; -; .
HOVERGEN	HBG051845; -; .
InParanoid	P48721; -; .
OrthoDB	EOG49KFQ6; -; .
PhylomeDB	P48721; -; .
NextBio	632983; -; .
ArrayExpress	P48721; -; .
Genevestigator	P48721; -; .
GermOnline	ENSRNOG00000019525; Rattus norvegicus; .
GO	GO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GO	GO:0051082; F:unfolded protein binding; IEA:InterPro; .
GO	GO:0006457; P:protein folding; IEA:InterPro; .
InterPro	IPR012725; Chaperone_DnaK; .
InterPro	IPR018181; Heat_shock_70_CS; .
InterPro	IPR001023; Hsp70; .
InterPro	IPR013126; Hsp_70; .
KO	K04043; -; .
PANTHER	PTHR19375; Hsp70; 1; .
Pfam	PF00012; HSP70; 1; .
PRINTS	PR00301; HEATSHOCK70; .
TIGRFAMs	TIGR02350; Prok_dnaK; 1; .
PROSITE	PS00297; HSP70_1; 1; .
PROSITE	PS00329; HSP70_2; 1; .
PROSITE	PS01036; HSP70_3; 1; .

Searching in 'World-2DPAGE Repository [0030]' for entry matching: P48721

World-2DPAGE Repository (0030): P48721

P48721

General information about the entry

Entry name

GRP75_RAT

Primary accession number

P48721

integrated into World-2DPAGE Repository (0030) on

November 9, 2010 (release 1)

2D Annotations were last modified on

June 21, 2011 (version 2)

General Annotations were last modified on

November 23, 2011 (version 2)

Name and origin of the protein

Description

Gene name

Name=Hspa9
Synonyms=Grp75, Hspa9a

Annotated species

Rattus norvegicus (Rat) [TaxID: 10116]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	2D-PAGE GEL CHARACTERIZATION DOI=10.1002/pmic.201000006; D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C. ''Two-dimensional gel proteome reference map of INS-1E cells'' Proteomics 11(7):1365-1369 (2011)

2D PAGE maps for identified proteins

RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7}
Rattus norvegicus (Rat)

RAT_INS1E_4-7

MAP LOCATIONS:

SPOT 539:

pI=5.39; Mw=80194

SPOT 549:

pI=5.47; Mw=79531

SPOT 638:

pI=5.28; Mw=75563

SPOT 685:

pI=5.35; Mw=74113

SPOT 698:

pI=5.28; Mw=73806

IDENTIFICATION: SPOT 539: Mascot protein score=454. Number of peptides=10 [1]
SPOT 549: Mascot protein score=439. Number of peptides=11 [1]; SPOT 638: Mascot protein score=78. Number of peptides=5 [1]; SPOT 685: Mascot protein score=484. Number of peptides=12 [1]; SPOT 698: Mascot protein score=631. Number of peptides=10 [1].
MAPPING (identification):

SPOT 539: Tandem mass spectrometry [1];
SPOT 549: Tandem mass spectrometry [1];
SPOT 638: Tandem mass spectrometry [1];
SPOT 685: Tandem mass spectrometry [1];
SPOT 698: Tandem mass spectrometry [1].

Copyright

Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium

Cross-references

UniProtKB/Swiss-Prot

World-2DPAGE Repository