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Searching in 'World-2DPAGE Repository [0030]' for entry matching: P48721




World-2DPAGE Repository (0030):  P48721


P48721


General information about the entry
View entry in simple text format
Entry nameGRP75_RAT
Primary accession numberP48721
integrated into World-2DPAGE Repository (0030) on November 9, 2010 (release 1)
2D Annotations were last modified onJune 21, 2011 (version 2)
General Annotations were last modified on November 23, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Stress-70 protein, mitochondrial; AltName: Full=75 kDa glucose-regulated protein; Short=GRP-75; AltName: Full=Heat shock 70 kDa protein 9; AltName: Full=Mortalin; AltName: Full=Peptide-binding protein 74; Short=PBP74; AltName: Full=mtHSP70; Flags: Precursor;.
Gene nameName=Hspa9
Synonyms=Grp75, Hspa9a
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000006;
D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C.
''Two-dimensional gel proteome reference map of INS-1E cells''
Proteomics 11(7):1365-1369 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7}
Rattus norvegicus (Rat)
RAT_INS1E_4-7
  map experimental info
 
RAT_INS1E_4-7

MAP LOCATIONS:
pI=5.39; Mw=80194  [identification data]
pI=5.47; Mw=79531  [identification data]
pI=5.28; Mw=75563  [identification data]
pI=5.35; Mw=74113  [identification data]
pI=5.28; Mw=73806  [identification data]

IDENTIFICATION: SPOT 539: Mascot protein score=454. Number of peptides=10 [1]
SPOT 549: Mascot protein score=439. Number of peptides=11 [1]; SPOT 638: Mascot protein score=78. Number of peptides=5 [1]; SPOT 685: Mascot protein score=484. Number of peptides=12 [1]; SPOT 698: Mascot protein score=631. Number of peptides=10 [1].
MAPPING (identification):
SPOT 539: Tandem mass spectrometry [1];
SPOT 549: Tandem mass spectrometry [1];
SPOT 638: Tandem mass spectrometry [1];
SPOT 685: Tandem mass spectrometry [1];
SPOT 698: Tandem mass spectrometry [1].

Copyright
Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium
Cross-references
UniProtKB/Swiss-ProtP48721; GRP75_RAT.
World-2DPAGE RepositoryP48721; GRP75_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameGRP75_RAT
Primary accession numberP48721
Sequence was last modified on October 17, 2006 (version 3)
Annotations were last modified on November 16, 2011 (version 95)
Name and origin of the protein
DescriptionRecName: Full=Stress-70 protein, mitochondrial; AltName: Full=75 kDa glucose-regulated protein; Short=GRP-75; AltName: Full=Heat shock 70 kDa protein 9; AltName: Full=Mortalin; AltName: Full=Peptide-binding protein 74; Short=PBP74; AltName: Full=mtHSP70; Flags: Precursor;
Gene nameName=Hspa9
Synonyms=Grp75, Hspa9a
Encoded onName=Hspa9; Synonyms=Grp75, Hspa9a
KeywordsAcetylation; ATP-binding; Chaperone; Complete proteome; Direct protein sequencing; Mitochondrion; Nucleotide-binding; Phosphoprotein; Reference proteome; Transit peptide.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLS75280; AAB33049.1; -; mRNA
EMBLS78556; AAB34982.1; -; mRNA
IPIIPI00363265; -; .
PIRI56581; I56581; .
RefSeqNP_001094128.1; NM_001100658.1; .
UniGeneRn.7535; -; .
ProteinModelPortalP48721; -; .
SMRP48721; 55-580; .
IntActP48721; 2; .
MINTMINT-4592308; -; .
STRINGP48721; -; .
PhosphoSiteP48721; -; .
World-2DPAGE0004:P48721; -; .
PRIDEP48721; -; .
GeneID291671; -; .
KEGGrno:291671; -; .
UCSCS78556; rat; .
CTD3313; -; .
RGD1311806; Hspa9; .
eggNOGmaNOG05443; -; .
GeneTreeENSGT00550000074813; -; .
HOVERGENHBG051845; -; .
InParanoidP48721; -; .
OrthoDBEOG49KFQ6; -; .
PhylomeDBP48721; -; .
NextBio632983; -; .
ArrayExpressP48721; -; .
GenevestigatorP48721; -; .
GermOnlineENSRNOG00000019525; Rattus norvegicus; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0051082; F:unfolded protein binding; IEA:InterPro; .
GOGO:0006457; P:protein folding; IEA:InterPro; .
InterProIPR012725; Chaperone_DnaK; .
InterProIPR018181; Heat_shock_70_CS; .
InterProIPR001023; Hsp70; .
InterProIPR013126; Hsp_70; .
KOK04043; -; .
PANTHERPTHR19375; Hsp70; 1; .
PfamPF00012; HSP70; 1; .
PRINTSPR00301; HEATSHOCK70; .
TIGRFAMsTIGR02350; Prok_dnaK; 1; .
PROSITEPS00297; HSP70_1; 1; .
PROSITEPS00329; HSP70_2; 1; .
PROSITEPS01036; HSP70_3; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server