World-2DPAGE Repository

Entry name

ODPB_RAT

Primary accession number

P49432

integrated into World-2DPAGE Repository (0004) on

October 13, 2008 (release 1)

2D Annotations were last modified on

May 18, 2011 (version 2)

General Annotations were last modified on

December 22, 2011 (version 2)

Name and origin of the protein

Description

RecName: Full=Pyruvate dehydrogenase E1 component subunit beta, mitochondrial; Short=PDHE1-B; EC=1.2.4.1; Flags: Precursor;.

Gene name

Name=Pdhb

Annotated species

Rattus norvegicus (Rat) [TaxID: 10116]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	MAPPING ON GEL PubMed=19343716; DOI=10.1002/pmic.200800664; [NCBI, EBI, Israel, Japan] Maurya D.K., Sundaram C.S., Bhargava P. ''Proteome profile of the mature rat olfactory bulb'' Proteomics 9(1):2593-2599 (2009)

2D PAGE maps for identified proteins

OB21DSUB {2-DE gel for Olfactory bulb proteome}
Rattus norvegicus (Rat)
Tissue: Olfactory bulb

OB21DSUB

MAP LOCATIONS:

SPOT 532:

pI=5.38; Mw=37000

MAPPING (identification):

SPOT 532: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Purnima Bhargava, Centre for Cellular and Molecular Biology, India

Cross-references

UniProtKB/Swiss-Prot

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry name	ODPB_RAT
Primary accession number	P49432
Secondary accession number(s)	Q6AY95
Sequence was last modified on	January 9, 2007 (version 2)
Annotations were last modified on	November 16, 2011 (version 95)
Name and origin of the protein
Description	RecName: Full=Pyruvate dehydrogenase E1 component subunit beta, mitochondrial; Short=PDHE1-B; EC=1.2.4.1; Flags: Precursor;
Gene name	Name=Pdhb
Encoded on	Name=Pdhb
Keywords	Complete proteome; Direct protein sequencing; Glycolysis; Mitochondrion; Oxidoreductase; Phosphoprotein; Pyruvate; Reference proteome; Thiamine pyrophosphate; Transit peptide.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	BC079137; AAH79137.1; -; mRNA
IPI	IPI00194324; -; .
PIR	S15892; S15892; .
RefSeq	NP_001007621.1; NM_001007620.1; .
UniGene	Rn.102424; -; .
ProteinModelPortal	P49432; -; .
SMR	P49432; 30-359; .
IntAct	P49432; 1; .
MINT	MINT-4592348; -; .
STRING	P49432; -; .
PhosphoSite	P49432; -; .
UCD-2DPAGE	P49432; -; .
World-2DPAGE	0004:P49432; -; .
PRIDE	P49432; -; .
Ensembl	ENSRNOT00000010545; ENSRNOP00000010545; ENSRNOG00000007895; .
GeneID	289950; -; .
KEGG	rno:289950; -; .
NMPDR	fig\|10116.3.peg.11094; -; .
UCSC	NM_001007620; rat; .
CTD	5162; -; .
RGD	1359146; Pdhb; .
eggNOG	roNOG10273; -; .
GeneTree	ENSGT00530000063423; -; .
HOVERGEN	HBG000917; -; .
InParanoid	P49432; -; .
OMA	MSGGLQS; -; .
OrthoDB	EOG4CJVHD; -; .
PhylomeDB	P49432; -; .
NextBio	630523; -; .
ArrayExpress	P49432; -; .
Genevestigator	P49432; -; .
GermOnline	ENSRNOG00000007895; Rattus norvegicus; .
GO	GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell; .
GO	GO:0045254; C:pyruvate dehydrogenase complex; IDA:RGD; .
GO	GO:0004739; F:pyruvate dehydrogenase (acetyl-transferring) activity; IDA:RGD; .
GO	GO:0006086; P:acetyl-CoA biosynthetic process from pyruvate; IDA:RGD; .
GO	GO:0006096; P:glycolysis; IEA:UniProtKB-KW; .
InterPro	IPR009014; Transketo_C/Pyr-ferredox_oxred; .
InterPro	IPR015941; Transketolase-like_C; .
InterPro	IPR005475; Transketolase-like_Pyr-bd; .
InterPro	IPR005476; Transketolase_C; .
Gene3D	G3DSA:3.40.50.920; Transketo_C_like; 1; .
KO	K00162; -; .
Pfam	PF02779; Transket_pyr; 1; .
Pfam	PF02780; Transketolase_C; 1; .
SMART	SM00861; Transket_pyr; 1; .
SUPFAM	SSF52922; Transketo_C_like; 1; .

Searching in 'World-2DPAGE Repository [0014]' for entry matching: P49432

World-2DPAGE Repository (0014): P49432

P49432

General information about the entry

Entry name

ODPB_RAT

Primary accession number

P49432

integrated into World-2DPAGE Repository (0014) on

October 13, 2008 (release 1)

2D Annotations were last modified on

June 1, 2011 (version 3)

General Annotations were last modified on

November 25, 2011 (version 2)

Name and origin of the protein

Description

RecName: Full=Pyruvate dehydrogenase E1 component subunit beta, mitochondrial; Short=PDHE1-B; EC=1.2.4.1; Flags: Precursor;.

Gene name

Name=Pdhb

Annotated species

Rattus norvegicus (Rat) [TaxID: 10116]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	2D-PAGE GEL CHARACTERIZATION DOI=10.1002/pmic.200900680; Maurya D.K., Sundaram C.S., Bhargava P. ''Proteome profile of whole cerebellum of the mature rat'' Proteomics 10(23):4311-4319 (2010)

2D PAGE maps for identified proteins

RAT_CEREBELLUM_21D {Proteome of 21 days old rat cerebellum}
Rattus norvegicus (Rat)
Tissue: Cerebellum

RAT_CEREBELLUM_21D

MAP LOCATIONS:

SPOT 3039:

pI=5.41; Mw=38000

IDENTIFICATION: SPOT 3039: SeqCov=30%. MascotPMF=54 [1].
MAPPING (identification):

SPOT 3039: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Purnima Bhargava, Centre for Cellular and Molecular Biology, India

Cross-references

PRIDE

10017.

UniProtKB/Swiss-Prot

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry name	ODPB_RAT
Primary accession number	P49432
Secondary accession number(s)	Q6AY95
Sequence was last modified on	January 9, 2007 (version 2)
Annotations were last modified on	November 16, 2011 (version 95)
Name and origin of the protein
Description	RecName: Full=Pyruvate dehydrogenase E1 component subunit beta, mitochondrial; Short=PDHE1-B; EC=1.2.4.1; Flags: Precursor;
Gene name	Name=Pdhb
Encoded on	Name=Pdhb
Keywords	Complete proteome; Direct protein sequencing; Glycolysis; Mitochondrion; Oxidoreductase; Phosphoprotein; Pyruvate; Reference proteome; Thiamine pyrophosphate; Transit peptide.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	BC079137; AAH79137.1; -; mRNA
IPI	IPI00194324; -; .
PIR	S15892; S15892; .
RefSeq	NP_001007621.1; NM_001007620.1; .
UniGene	Rn.102424; -; .
ProteinModelPortal	P49432; -; .
SMR	P49432; 30-359; .
IntAct	P49432; 1; .
MINT	MINT-4592348; -; .
STRING	P49432; -; .
PhosphoSite	P49432; -; .
UCD-2DPAGE	P49432; -; .
World-2DPAGE	0004:P49432; -; .
PRIDE	P49432; -; .
Ensembl	ENSRNOT00000010545; ENSRNOP00000010545; ENSRNOG00000007895; .
GeneID	289950; -; .
KEGG	rno:289950; -; .
NMPDR	fig\|10116.3.peg.11094; -; .
UCSC	NM_001007620; rat; .
CTD	5162; -; .
RGD	1359146; Pdhb; .
eggNOG	roNOG10273; -; .
GeneTree	ENSGT00530000063423; -; .
HOVERGEN	HBG000917; -; .
InParanoid	P49432; -; .
OMA	MSGGLQS; -; .
OrthoDB	EOG4CJVHD; -; .
PhylomeDB	P49432; -; .
NextBio	630523; -; .
ArrayExpress	P49432; -; .
Genevestigator	P49432; -; .
GermOnline	ENSRNOG00000007895; Rattus norvegicus; .
GO	GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell; .
GO	GO:0045254; C:pyruvate dehydrogenase complex; IDA:RGD; .
GO	GO:0004739; F:pyruvate dehydrogenase (acetyl-transferring) activity; IDA:RGD; .
GO	GO:0006086; P:acetyl-CoA biosynthetic process from pyruvate; IDA:RGD; .
GO	GO:0006096; P:glycolysis; IEA:UniProtKB-KW; .
InterPro	IPR009014; Transketo_C/Pyr-ferredox_oxred; .
InterPro	IPR015941; Transketolase-like_C; .
InterPro	IPR005475; Transketolase-like_Pyr-bd; .
InterPro	IPR005476; Transketolase_C; .
Gene3D	G3DSA:3.40.50.920; Transketo_C_like; 1; .
KO	K00162; -; .
Pfam	PF02779; Transket_pyr; 1; .
Pfam	PF02780; Transketolase_C; 1; .
SMART	SM00861; Transket_pyr; 1; .
SUPFAM	SSF52922; Transketo_C_like; 1; .

Searching in 'World-2DPAGE Repository [0019]' for entry matching: P49432

World-2DPAGE Repository (0019): P49432

P49432

General information about the entry

Entry name

ODPB_RAT

Primary accession number

P49432

integrated into World-2DPAGE Repository (0019) on

December 9, 2009 (release 1)

2D Annotations were last modified on

May 30, 2011 (version 2)

General Annotations were last modified on

January 13, 2012 (version 2)

Name and origin of the protein

Description

RecName: Full=Pyruvate dehydrogenase E1 component subunit beta, mitochondrial; Short=PDHE1-B; EC=1.2.4.1; Flags: Precursor;.

Gene name

Name=Pdhb

Annotated species

Rattus norvegicus (Rat) [TaxID: 10116]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	2D-PAGE GEL CHARACTERIZATION DOI=10.1111/j.1471-4159.2010.06719.x; VanGuilder H.D., Yan H., Farley J.A., Sonntag W.E., Freeman W.M. ''Aging alters the expression of neurotransmission-regulating proteins in the hippocampal synaptoproteome'' J of Neurochemistry 113(6):1577-1588 (2010)

2D PAGE maps for identified proteins

HIPP_SYNAP {Rat hippocampal synaptoproteome}
Rattus norvegicus (Rat)
Tissue: Hippocampus

HIPP_SYNAP

MAP LOCATIONS:

SPOT 639:

pI=6.20; Mw=39299

IDENTIFICATION: SPOT 639: Peptides number=10. SeqCov=35.9%. MOWSE score=1.62E+7 [1].
MAPPING (identification):

SPOT 639: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Willard Freeman, Penn State College of Medicine, USA

Cross-references

UniProtKB/Swiss-Prot

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry name	ODPB_RAT
Primary accession number	P49432
Secondary accession number(s)	Q6AY95
Sequence was last modified on	January 9, 2007 (version 2)
Annotations were last modified on	November 16, 2011 (version 95)
Name and origin of the protein
Description	RecName: Full=Pyruvate dehydrogenase E1 component subunit beta, mitochondrial; Short=PDHE1-B; EC=1.2.4.1; Flags: Precursor;
Gene name	Name=Pdhb
Encoded on	Name=Pdhb
Keywords	Complete proteome; Direct protein sequencing; Glycolysis; Mitochondrion; Oxidoreductase; Phosphoprotein; Pyruvate; Reference proteome; Thiamine pyrophosphate; Transit peptide.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	BC079137; AAH79137.1; -; mRNA
IPI	IPI00194324; -; .
PIR	S15892; S15892; .
RefSeq	NP_001007621.1; NM_001007620.1; .
UniGene	Rn.102424; -; .
ProteinModelPortal	P49432; -; .
SMR	P49432; 30-359; .
IntAct	P49432; 1; .
MINT	MINT-4592348; -; .
STRING	P49432; -; .
PhosphoSite	P49432; -; .
UCD-2DPAGE	P49432; -; .
World-2DPAGE	0004:P49432; -; .
PRIDE	P49432; -; .
Ensembl	ENSRNOT00000010545; ENSRNOP00000010545; ENSRNOG00000007895; .
GeneID	289950; -; .
KEGG	rno:289950; -; .
NMPDR	fig\|10116.3.peg.11094; -; .
UCSC	NM_001007620; rat; .
CTD	5162; -; .
RGD	1359146; Pdhb; .
eggNOG	roNOG10273; -; .
GeneTree	ENSGT00530000063423; -; .
HOVERGEN	HBG000917; -; .
InParanoid	P49432; -; .
OMA	MSGGLQS; -; .
OrthoDB	EOG4CJVHD; -; .
PhylomeDB	P49432; -; .
NextBio	630523; -; .
ArrayExpress	P49432; -; .
Genevestigator	P49432; -; .
GermOnline	ENSRNOG00000007895; Rattus norvegicus; .
GO	GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell; .
GO	GO:0045254; C:pyruvate dehydrogenase complex; IDA:RGD; .
GO	GO:0004739; F:pyruvate dehydrogenase (acetyl-transferring) activity; IDA:RGD; .
GO	GO:0006086; P:acetyl-CoA biosynthetic process from pyruvate; IDA:RGD; .
GO	GO:0006096; P:glycolysis; IEA:UniProtKB-KW; .
InterPro	IPR009014; Transketo_C/Pyr-ferredox_oxred; .
InterPro	IPR015941; Transketolase-like_C; .
InterPro	IPR005475; Transketolase-like_Pyr-bd; .
InterPro	IPR005476; Transketolase_C; .
Gene3D	G3DSA:3.40.50.920; Transketo_C_like; 1; .
KO	K00162; -; .
Pfam	PF02779; Transket_pyr; 1; .
Pfam	PF02780; Transketolase_C; 1; .
SMART	SM00861; Transket_pyr; 1; .
SUPFAM	SSF52922; Transketo_C_like; 1; .

Searching in 'World-2DPAGE Repository [0030]' for entry matching: P49432

World-2DPAGE Repository (0030): P49432

P49432

General information about the entry

Entry name

ODPB_RAT

Primary accession number

P49432

integrated into World-2DPAGE Repository (0030) on

November 9, 2010 (release 1)

2D Annotations were last modified on

June 21, 2011 (version 2)

General Annotations were last modified on

November 23, 2011 (version 2)

Name and origin of the protein

Description

RecName: Full=Pyruvate dehydrogenase E1 component subunit beta, mitochondrial; Short=PDHE1-B; EC=1.2.4.1; Flags: Precursor;.

Gene name

Name=Pdhb

Annotated species

Rattus norvegicus (Rat) [TaxID: 10116]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	2D-PAGE GEL CHARACTERIZATION DOI=10.1002/pmic.201000006; D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C. ''Two-dimensional gel proteome reference map of INS-1E cells'' Proteomics 11(7):1365-1369 (2011)

2D PAGE maps for identified proteins

RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7}
Rattus norvegicus (Rat)

RAT_INS1E_4-7

MAP LOCATIONS:

SPOT 1683:

pI=5.35; Mw=40827

IDENTIFICATION: SPOT 1683: Mascot protein score=162. Number of peptides=5 [1].
MAPPING (identification):

SPOT 1683: Tandem mass spectrometry [1].

Copyright

Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium

Cross-references

UCD-2DPAGE

UniProtKB/Swiss-Prot

World-2DPAGE Repository