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Searching in 'World-2DPAGE Repository [0004]' for entry matching: P52873




World-2DPAGE Repository (0004):  P52873


P52873


General information about the entry
View entry in simple text format
Entry namePYC_RAT
Primary accession numberP52873
integrated into World-2DPAGE Repository (0004) on October 13, 2008 (release 1)
2D Annotations were last modified onMay 18, 2011 (version 2)
General Annotations were last modified on December 22, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Pyruvate carboxylase, mitochondrial; EC=6.4.1.1; AltName: Full=Pyruvic carboxylase; Short=PCB; Flags: Precursor;.
Gene nameName=Pc
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
References
[1]   MAPPING ON GEL
PubMed=19343716; DOI=10.1002/pmic.200800664; [NCBI, EBI, Israel, Japan]
Maurya D.K., Sundaram C.S., Bhargava P.
''Proteome profile of the mature rat olfactory bulb''
Proteomics 9(1):2593-2599 (2009)
2D PAGE maps for identified proteins
How to interpret a protein

OB21DSUB {2-DE gel for Olfactory bulb proteome}
Rattus norvegicus (Rat)
Tissue: Olfactory bulb
OB21DSUB
  map experimental info
 
OB21DSUB

MAP LOCATIONS:
pI=6.23; Mw=95000  [identification data]

MAPPING (identification):
SPOT 71: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright
Data from Dr. Purnima Bhargava, Centre for Cellular and Molecular Biology, India
Cross-references
UniProtKB/Swiss-ProtP52873; PYC_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry namePYC_RAT
Primary accession numberP52873
Secondary accession number(s) Q5RKM0 Q64555
Sequence was last modified on May 1, 2007 (version 2)
Annotations were last modified on November 16, 2011 (version 112)
Name and origin of the protein
DescriptionRecName: Full=Pyruvate carboxylase, mitochondrial; EC=6.4.1.1; AltName: Full=Pyruvic carboxylase; Short=PCB; Flags: Precursor;
Gene nameName=Pc
Encoded onName=Pc
KeywordsAcetylation; ATP-binding; Biotin; Complete proteome; Direct protein sequencing; Gluconeogenesis; Ligase; Lipid synthesis; Manganese; Metal-binding; Mitochondrion; Multifunctional enzyme; Nucleotide-binding; Phosphoprotein; Pyruvate; Reference proteome; Transit peptide.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLU32314; AAA96256.1; -; mRNA
EMBLU36585; AAC52668.1; -; mRNA
EMBLBC085680; AAH85680.1; -; mRNA
IPIIPI00210435; -; .
PIRS68252; JC4391; .
RefSeqNP_036876.2; NM_012744.2; .
UniGeneRn.11094; -; .
ProteinModelPortalP52873; -; .
SMRP52873; 35-490; 494-1178; .
MINTMINT-4592965; -; .
STRINGP52873; -; .
World-2DPAGE0004:P52873; -; .
PRIDEP52873; -; .
EnsemblENSRNOT00000026316; ENSRNOP00000026316; ENSRNOG00000019372; .
GeneID25104; -; .
KEGGrno:25104; -; .
NMPDRfig|10116.3.peg.3454; -; .
UCSCNM_012744; rat; .
CTD5091; -; .
RGD3262; Pc; .
eggNOGroNOG05375; -; .
GeneTreeENSGT00550000074986; -; .
HOVERGENHBG008340; -; .
InParanoidP52873; -; .
OMAYKADEAY; -; .
OrthoDBEOG4ZCT3P; -; .
PhylomeDBP52873; -; .
NextBio605431; -; .
ArrayExpressP52873; -; .
GenevestigatorP52873; -; .
GermOnlineENSRNOG00000019372; Rattus norvegicus; .
GOGO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell; .
GOGO:0005625; C:soluble fraction; IDA:RGD; .
GOGO:0005524; F:ATP binding; IDA:RGD; .
GOGO:0009374; F:biotin binding; IMP:RGD; .
GOGO:0004075; F:biotin carboxylase activity; IEA:InterPro; .
GOGO:0046872; F:metal ion binding; IEA:UniProtKB-KW; .
GOGO:0004736; F:pyruvate carboxylase activity; IDA:RGD; .
GOGO:0006094; P:gluconeogenesis; IDA:RGD; .
GOGO:0008610; P:lipid biosynthetic process; IEA:UniProtKB-KW; .
GOGO:0006107; P:oxaloacetate metabolic process; IDA:RGD; .
GOGO:0006090; P:pyruvate metabolic process; IDA:RGD; .
InterProIPR013785; Aldolase_TIM; .
InterProIPR011761; ATP-grasp; .
InterProIPR013815; ATP_grasp_subdomain_1; .
InterProIPR013816; ATP_grasp_subdomain_2; .
InterProIPR001882; Biotin_BS; .
InterProIPR011764; Biotin_carboxylation_dom; .
InterProIPR005482; Biotin_COase_C; .
InterProIPR000089; Biotin_lipoyl; .
InterProIPR005479; CarbamoylP_synth_lsu_ATP-bd; .
InterProIPR005481; CarbamoylP_synth_lsu_N; .
InterProIPR003379; Carboxylase_cons_dom; .
InterProIPR013817; Pre-ATP_grasp; .
InterProIPR016185; PreATP-grasp-like; .
InterProIPR000891; PYR_CT; .
InterProIPR005930; Pyruv_COase; .
InterProIPR011054; Rudment_hybrid_motif; .
InterProIPR011053; Single_hybrid_motif; .
Gene3DG3DSA:3.20.20.70; Aldolase_TIM; 1; .
Gene3DG3DSA:3.30.1490.20; ATP_grasp_subdomain_1; 1; .
Gene3DG3DSA:3.30.470.20; ATP_grasp_subdomain_2; 1; .
Gene3DG3DSA:3.40.50.20; Pre-ATP_grasp; 1; .
KOK01958; -; .
PANTHERPTHR18866:SF10; PTHR18866:SF10; 1; .
PfamPF02785; Biotin_carb_C; 1; .
PfamPF00364; Biotin_lipoyl; 1; .
PfamPF00289; CPSase_L_chain; 1; .
PfamPF02786; CPSase_L_D2; 1; .
PfamPF00682; HMGL-like; 1; .
PfamPF02436; PYC_OADA; 1; .
PIRSFPIRSF001594; Pyruv_carbox; 1; .
SMARTSM00878; Biotin_carb_C; 1; .
SUPFAMSSF51230; Hybrid_motif; 1; .
SUPFAMSSF52440; PreATP-grasp-like; 1; .
SUPFAMSSF51246; Rudmnt_hyb_motif; 1; .
TIGRFAMsTIGR01235; Pyruv_carbox; 1; .
PROSITEPS50975; ATP_GRASP; 1; .
PROSITEPS50979; BC; 1; .
PROSITEPS00188; BIOTIN; 1; .
PROSITEPS50968; BIOTINYL_LIPOYL; 1; .
PROSITEPS50991; PYR_CT; 1; .





Searching in 'World-2DPAGE Repository [0014]' for entry matching: P52873




World-2DPAGE Repository (0014):  P52873


P52873


General information about the entry
View entry in simple text format
Entry namePYC_RAT
Primary accession numberP52873
integrated into World-2DPAGE Repository (0014) on October 13, 2008 (release 1)
2D Annotations were last modified onJune 1, 2011 (version 3)
General Annotations were last modified on November 25, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Pyruvate carboxylase, mitochondrial; EC=6.4.1.1; AltName: Full=Pyruvic carboxylase; Short=PCB; Flags: Precursor;.
Gene nameName=Pc
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.200900680;
Maurya D.K., Sundaram C.S., Bhargava P.
''Proteome profile of whole cerebellum of the mature rat''
Proteomics 10(23):4311-4319 (2010)
2D PAGE maps for identified proteins
How to interpret a protein

RAT_CEREBELLUM_21D {Proteome of 21 days old rat cerebellum}
Rattus norvegicus (Rat)
Tissue: Cerebellum
RAT_CEREBELLUM_21D
  map experimental info
 
RAT_CEREBELLUM_21D

MAP LOCATIONS:
pI=6.32; Mw=90000  [identification data]

IDENTIFICATION: SPOT 2660: SeqCov=35%. MascotPMF=248 [1].
MAPPING (identification):
SPOT 2660: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright
Data from Dr. Purnima Bhargava, Centre for Cellular and Molecular Biology, India
Cross-references
PRIDE10017.
UniProtKB/Swiss-ProtP52873; PYC_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry namePYC_RAT
Primary accession numberP52873
Secondary accession number(s) Q5RKM0 Q64555
Sequence was last modified on May 1, 2007 (version 2)
Annotations were last modified on November 16, 2011 (version 112)
Name and origin of the protein
DescriptionRecName: Full=Pyruvate carboxylase, mitochondrial; EC=6.4.1.1; AltName: Full=Pyruvic carboxylase; Short=PCB; Flags: Precursor;
Gene nameName=Pc
Encoded onName=Pc
KeywordsAcetylation; ATP-binding; Biotin; Complete proteome; Direct protein sequencing; Gluconeogenesis; Ligase; Lipid synthesis; Manganese; Metal-binding; Mitochondrion; Multifunctional enzyme; Nucleotide-binding; Phosphoprotein; Pyruvate; Reference proteome; Transit peptide.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLU32314; AAA96256.1; -; mRNA
EMBLU36585; AAC52668.1; -; mRNA
EMBLBC085680; AAH85680.1; -; mRNA
IPIIPI00210435; -; .
PIRS68252; JC4391; .
RefSeqNP_036876.2; NM_012744.2; .
UniGeneRn.11094; -; .
ProteinModelPortalP52873; -; .
SMRP52873; 35-490; 494-1178; .
MINTMINT-4592965; -; .
STRINGP52873; -; .
World-2DPAGE0004:P52873; -; .
PRIDEP52873; -; .
EnsemblENSRNOT00000026316; ENSRNOP00000026316; ENSRNOG00000019372; .
GeneID25104; -; .
KEGGrno:25104; -; .
NMPDRfig|10116.3.peg.3454; -; .
UCSCNM_012744; rat; .
CTD5091; -; .
RGD3262; Pc; .
eggNOGroNOG05375; -; .
GeneTreeENSGT00550000074986; -; .
HOVERGENHBG008340; -; .
InParanoidP52873; -; .
OMAYKADEAY; -; .
OrthoDBEOG4ZCT3P; -; .
PhylomeDBP52873; -; .
NextBio605431; -; .
ArrayExpressP52873; -; .
GenevestigatorP52873; -; .
GermOnlineENSRNOG00000019372; Rattus norvegicus; .
GOGO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell; .
GOGO:0005625; C:soluble fraction; IDA:RGD; .
GOGO:0005524; F:ATP binding; IDA:RGD; .
GOGO:0009374; F:biotin binding; IMP:RGD; .
GOGO:0004075; F:biotin carboxylase activity; IEA:InterPro; .
GOGO:0046872; F:metal ion binding; IEA:UniProtKB-KW; .
GOGO:0004736; F:pyruvate carboxylase activity; IDA:RGD; .
GOGO:0006094; P:gluconeogenesis; IDA:RGD; .
GOGO:0008610; P:lipid biosynthetic process; IEA:UniProtKB-KW; .
GOGO:0006107; P:oxaloacetate metabolic process; IDA:RGD; .
GOGO:0006090; P:pyruvate metabolic process; IDA:RGD; .
InterProIPR013785; Aldolase_TIM; .
InterProIPR011761; ATP-grasp; .
InterProIPR013815; ATP_grasp_subdomain_1; .
InterProIPR013816; ATP_grasp_subdomain_2; .
InterProIPR001882; Biotin_BS; .
InterProIPR011764; Biotin_carboxylation_dom; .
InterProIPR005482; Biotin_COase_C; .
InterProIPR000089; Biotin_lipoyl; .
InterProIPR005479; CarbamoylP_synth_lsu_ATP-bd; .
InterProIPR005481; CarbamoylP_synth_lsu_N; .
InterProIPR003379; Carboxylase_cons_dom; .
InterProIPR013817; Pre-ATP_grasp; .
InterProIPR016185; PreATP-grasp-like; .
InterProIPR000891; PYR_CT; .
InterProIPR005930; Pyruv_COase; .
InterProIPR011054; Rudment_hybrid_motif; .
InterProIPR011053; Single_hybrid_motif; .
Gene3DG3DSA:3.20.20.70; Aldolase_TIM; 1; .
Gene3DG3DSA:3.30.1490.20; ATP_grasp_subdomain_1; 1; .
Gene3DG3DSA:3.30.470.20; ATP_grasp_subdomain_2; 1; .
Gene3DG3DSA:3.40.50.20; Pre-ATP_grasp; 1; .
KOK01958; -; .
PANTHERPTHR18866:SF10; PTHR18866:SF10; 1; .
PfamPF02785; Biotin_carb_C; 1; .
PfamPF00364; Biotin_lipoyl; 1; .
PfamPF00289; CPSase_L_chain; 1; .
PfamPF02786; CPSase_L_D2; 1; .
PfamPF00682; HMGL-like; 1; .
PfamPF02436; PYC_OADA; 1; .
PIRSFPIRSF001594; Pyruv_carbox; 1; .
SMARTSM00878; Biotin_carb_C; 1; .
SUPFAMSSF51230; Hybrid_motif; 1; .
SUPFAMSSF52440; PreATP-grasp-like; 1; .
SUPFAMSSF51246; Rudmnt_hyb_motif; 1; .
TIGRFAMsTIGR01235; Pyruv_carbox; 1; .
PROSITEPS50975; ATP_GRASP; 1; .
PROSITEPS50979; BC; 1; .
PROSITEPS00188; BIOTIN; 1; .
PROSITEPS50968; BIOTINYL_LIPOYL; 1; .
PROSITEPS50991; PYR_CT; 1; .





Searching in 'World-2DPAGE Repository [0019]' for entry matching: P52873




World-2DPAGE Repository (0019):  P52873


P52873


General information about the entry
View entry in simple text format
Entry namePYC_RAT
Primary accession numberP52873
integrated into World-2DPAGE Repository (0019) on December 9, 2009 (release 1)
2D Annotations were last modified onMay 30, 2011 (version 2)
General Annotations were last modified on January 13, 2012 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Pyruvate carboxylase, mitochondrial; EC=6.4.1.1; AltName: Full=Pyruvic carboxylase; Short=PCB; Flags: Precursor;.
Gene nameName=Pc
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1111/j.1471-4159.2010.06719.x;
VanGuilder H.D., Yan H., Farley J.A., Sonntag W.E., Freeman W.M.
''Aging alters the expression of neurotransmission-regulating proteins in the hippocampal synaptoproteome''
J of Neurochemistry 113(6):1577-1588 (2010)
2D PAGE maps for identified proteins
How to interpret a protein

HIPP_SYNAP {Rat hippocampal synaptoproteome}
Rattus norvegicus (Rat)
Tissue: Hippocampus
HIPP_SYNAP
  map experimental info
 
HIPP_SYNAP

MAP LOCATIONS:
pI=6.34; Mw=130435  [identification data]
pI=6.34; Mw=130435  [identification data]

IDENTIFICATION: SPOT 94: Peptides number=8. SeqCov=5.7%. MOWSE score=6.12E+3 [1]
SPOT 97: Peptides number=17. SeqCov=7.5%. MOWSE score=1.94E+3 [1].
MAPPING (identification):
SPOT 94: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 97: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright
Data from Dr. Willard Freeman, Penn State College of Medicine, USA
Cross-references
UniProtKB/Swiss-ProtP52873; PYC_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry namePYC_RAT
Primary accession numberP52873
Secondary accession number(s) Q5RKM0 Q64555
Sequence was last modified on May 1, 2007 (version 2)
Annotations were last modified on November 16, 2011 (version 112)
Name and origin of the protein
DescriptionRecName: Full=Pyruvate carboxylase, mitochondrial; EC=6.4.1.1; AltName: Full=Pyruvic carboxylase; Short=PCB; Flags: Precursor;
Gene nameName=Pc
Encoded onName=Pc
KeywordsAcetylation; ATP-binding; Biotin; Complete proteome; Direct protein sequencing; Gluconeogenesis; Ligase; Lipid synthesis; Manganese; Metal-binding; Mitochondrion; Multifunctional enzyme; Nucleotide-binding; Phosphoprotein; Pyruvate; Reference proteome; Transit peptide.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLU32314; AAA96256.1; -; mRNA
EMBLU36585; AAC52668.1; -; mRNA
EMBLBC085680; AAH85680.1; -; mRNA
IPIIPI00210435; -; .
PIRS68252; JC4391; .
RefSeqNP_036876.2; NM_012744.2; .
UniGeneRn.11094; -; .
ProteinModelPortalP52873; -; .
SMRP52873; 35-490; 494-1178; .
MINTMINT-4592965; -; .
STRINGP52873; -; .
World-2DPAGE0004:P52873; -; .
PRIDEP52873; -; .
EnsemblENSRNOT00000026316; ENSRNOP00000026316; ENSRNOG00000019372; .
GeneID25104; -; .
KEGGrno:25104; -; .
NMPDRfig|10116.3.peg.3454; -; .
UCSCNM_012744; rat; .
CTD5091; -; .
RGD3262; Pc; .
eggNOGroNOG05375; -; .
GeneTreeENSGT00550000074986; -; .
HOVERGENHBG008340; -; .
InParanoidP52873; -; .
OMAYKADEAY; -; .
OrthoDBEOG4ZCT3P; -; .
PhylomeDBP52873; -; .
NextBio605431; -; .
ArrayExpressP52873; -; .
GenevestigatorP52873; -; .
GermOnlineENSRNOG00000019372; Rattus norvegicus; .
GOGO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell; .
GOGO:0005625; C:soluble fraction; IDA:RGD; .
GOGO:0005524; F:ATP binding; IDA:RGD; .
GOGO:0009374; F:biotin binding; IMP:RGD; .
GOGO:0004075; F:biotin carboxylase activity; IEA:InterPro; .
GOGO:0046872; F:metal ion binding; IEA:UniProtKB-KW; .
GOGO:0004736; F:pyruvate carboxylase activity; IDA:RGD; .
GOGO:0006094; P:gluconeogenesis; IDA:RGD; .
GOGO:0008610; P:lipid biosynthetic process; IEA:UniProtKB-KW; .
GOGO:0006107; P:oxaloacetate metabolic process; IDA:RGD; .
GOGO:0006090; P:pyruvate metabolic process; IDA:RGD; .
InterProIPR013785; Aldolase_TIM; .
InterProIPR011761; ATP-grasp; .
InterProIPR013815; ATP_grasp_subdomain_1; .
InterProIPR013816; ATP_grasp_subdomain_2; .
InterProIPR001882; Biotin_BS; .
InterProIPR011764; Biotin_carboxylation_dom; .
InterProIPR005482; Biotin_COase_C; .
InterProIPR000089; Biotin_lipoyl; .
InterProIPR005479; CarbamoylP_synth_lsu_ATP-bd; .
InterProIPR005481; CarbamoylP_synth_lsu_N; .
InterProIPR003379; Carboxylase_cons_dom; .
InterProIPR013817; Pre-ATP_grasp; .
InterProIPR016185; PreATP-grasp-like; .
InterProIPR000891; PYR_CT; .
InterProIPR005930; Pyruv_COase; .
InterProIPR011054; Rudment_hybrid_motif; .
InterProIPR011053; Single_hybrid_motif; .
Gene3DG3DSA:3.20.20.70; Aldolase_TIM; 1; .
Gene3DG3DSA:3.30.1490.20; ATP_grasp_subdomain_1; 1; .
Gene3DG3DSA:3.30.470.20; ATP_grasp_subdomain_2; 1; .
Gene3DG3DSA:3.40.50.20; Pre-ATP_grasp; 1; .
KOK01958; -; .
PANTHERPTHR18866:SF10; PTHR18866:SF10; 1; .
PfamPF02785; Biotin_carb_C; 1; .
PfamPF00364; Biotin_lipoyl; 1; .
PfamPF00289; CPSase_L_chain; 1; .
PfamPF02786; CPSase_L_D2; 1; .
PfamPF00682; HMGL-like; 1; .
PfamPF02436; PYC_OADA; 1; .
PIRSFPIRSF001594; Pyruv_carbox; 1; .
SMARTSM00878; Biotin_carb_C; 1; .
SUPFAMSSF51230; Hybrid_motif; 1; .
SUPFAMSSF52440; PreATP-grasp-like; 1; .
SUPFAMSSF51246; Rudmnt_hyb_motif; 1; .
TIGRFAMsTIGR01235; Pyruv_carbox; 1; .
PROSITEPS50975; ATP_GRASP; 1; .
PROSITEPS50979; BC; 1; .
PROSITEPS00188; BIOTIN; 1; .
PROSITEPS50968; BIOTINYL_LIPOYL; 1; .
PROSITEPS50991; PYR_CT; 1; .





Searching in 'World-2DPAGE Repository [0030]' for entry matching: P52873




World-2DPAGE Repository (0030):  P52873


P52873


General information about the entry
View entry in simple text format
Entry namePYC_RAT
Primary accession numberP52873
integrated into World-2DPAGE Repository (0030) on November 9, 2010 (release 1)
2D Annotations were last modified onJune 21, 2011 (version 2)
General Annotations were last modified on November 23, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Pyruvate carboxylase, mitochondrial; EC=6.4.1.1; AltName: Full=Pyruvic carboxylase; Short=PCB; Flags: Precursor;.
Gene nameName=Pc
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000006;
D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C.
''Two-dimensional gel proteome reference map of INS-1E cells''
Proteomics 11(7):1365-1369 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7}
Rattus norvegicus (Rat)
RAT_INS1E_4-7
  map experimental info
 
RAT_INS1E_4-7

MAP LOCATIONS:
pI=6.23; Mw=106489  [identification data]
pI=6.48; Mw=100478  [identification data]
pI=6.18; Mw=100062  [identification data]

IDENTIFICATION: SPOT 66: Mascot protein score=186. Number of peptides=6 [1]
SPOT 167: Mascot protein score=30. Number of peptides=3 [1]; SPOT 169: Mascot protein score=42. Number of peptides=5 [1].
MAPPING (identification):
SPOT 66: Tandem mass spectrometry [1];
SPOT 167: Tandem mass spectrometry [1];
SPOT 169: Tandem mass spectrometry [1].

Copyright
Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium
Cross-references
UniProtKB/Swiss-ProtP52873; PYC_RAT.
World-2DPAGE RepositoryP52873; PYC_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry namePYC_RAT
Primary accession numberP52873
Secondary accession number(s) Q5RKM0 Q64555
Sequence was last modified on May 1, 2007 (version 2)
Annotations were last modified on November 16, 2011 (version 112)
Name and origin of the protein
DescriptionRecName: Full=Pyruvate carboxylase, mitochondrial; EC=6.4.1.1; AltName: Full=Pyruvic carboxylase; Short=PCB; Flags: Precursor;
Gene nameName=Pc
Encoded onName=Pc
KeywordsAcetylation; ATP-binding; Biotin; Complete proteome; Direct protein sequencing; Gluconeogenesis; Ligase; Lipid synthesis; Manganese; Metal-binding; Mitochondrion; Multifunctional enzyme; Nucleotide-binding; Phosphoprotein; Pyruvate; Reference proteome; Transit peptide.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLU32314; AAA96256.1; -; mRNA
EMBLU36585; AAC52668.1; -; mRNA
EMBLBC085680; AAH85680.1; -; mRNA
IPIIPI00210435; -; .
PIRS68252; JC4391; .
RefSeqNP_036876.2; NM_012744.2; .
UniGeneRn.11094; -; .
ProteinModelPortalP52873; -; .
SMRP52873; 35-490; 494-1178; .
MINTMINT-4592965; -; .
STRINGP52873; -; .
World-2DPAGE0004:P52873; -; .
PRIDEP52873; -; .
EnsemblENSRNOT00000026316; ENSRNOP00000026316; ENSRNOG00000019372; .
GeneID25104; -; .
KEGGrno:25104; -; .
NMPDRfig|10116.3.peg.3454; -; .
UCSCNM_012744; rat; .
CTD5091; -; .
RGD3262; Pc; .
eggNOGroNOG05375; -; .
GeneTreeENSGT00550000074986; -; .
HOVERGENHBG008340; -; .
InParanoidP52873; -; .
OMAYKADEAY; -; .
OrthoDBEOG4ZCT3P; -; .
PhylomeDBP52873; -; .
NextBio605431; -; .
ArrayExpressP52873; -; .
GenevestigatorP52873; -; .
GermOnlineENSRNOG00000019372; Rattus norvegicus; .
GOGO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell; .
GOGO:0005625; C:soluble fraction; IDA:RGD; .
GOGO:0005524; F:ATP binding; IDA:RGD; .
GOGO:0009374; F:biotin binding; IMP:RGD; .
GOGO:0004075; F:biotin carboxylase activity; IEA:InterPro; .
GOGO:0046872; F:metal ion binding; IEA:UniProtKB-KW; .
GOGO:0004736; F:pyruvate carboxylase activity; IDA:RGD; .
GOGO:0006094; P:gluconeogenesis; IDA:RGD; .
GOGO:0008610; P:lipid biosynthetic process; IEA:UniProtKB-KW; .
GOGO:0006107; P:oxaloacetate metabolic process; IDA:RGD; .
GOGO:0006090; P:pyruvate metabolic process; IDA:RGD; .
InterProIPR013785; Aldolase_TIM; .
InterProIPR011761; ATP-grasp; .
InterProIPR013815; ATP_grasp_subdomain_1; .
InterProIPR013816; ATP_grasp_subdomain_2; .
InterProIPR001882; Biotin_BS; .
InterProIPR011764; Biotin_carboxylation_dom; .
InterProIPR005482; Biotin_COase_C; .
InterProIPR000089; Biotin_lipoyl; .
InterProIPR005479; CarbamoylP_synth_lsu_ATP-bd; .
InterProIPR005481; CarbamoylP_synth_lsu_N; .
InterProIPR003379; Carboxylase_cons_dom; .
InterProIPR013817; Pre-ATP_grasp; .
InterProIPR016185; PreATP-grasp-like; .
InterProIPR000891; PYR_CT; .
InterProIPR005930; Pyruv_COase; .
InterProIPR011054; Rudment_hybrid_motif; .
InterProIPR011053; Single_hybrid_motif; .
Gene3DG3DSA:3.20.20.70; Aldolase_TIM; 1; .
Gene3DG3DSA:3.30.1490.20; ATP_grasp_subdomain_1; 1; .
Gene3DG3DSA:3.30.470.20; ATP_grasp_subdomain_2; 1; .
Gene3DG3DSA:3.40.50.20; Pre-ATP_grasp; 1; .
KOK01958; -; .
PANTHERPTHR18866:SF10; PTHR18866:SF10; 1; .
PfamPF02785; Biotin_carb_C; 1; .
PfamPF00364; Biotin_lipoyl; 1; .
PfamPF00289; CPSase_L_chain; 1; .
PfamPF02786; CPSase_L_D2; 1; .
PfamPF00682; HMGL-like; 1; .
PfamPF02436; PYC_OADA; 1; .
PIRSFPIRSF001594; Pyruv_carbox; 1; .
SMARTSM00878; Biotin_carb_C; 1; .
SUPFAMSSF51230; Hybrid_motif; 1; .
SUPFAMSSF52440; PreATP-grasp-like; 1; .
SUPFAMSSF51246; Rudmnt_hyb_motif; 1; .
TIGRFAMsTIGR01235; Pyruv_carbox; 1; .
PROSITEPS50975; ATP_GRASP; 1; .
PROSITEPS50979; BC; 1; .
PROSITEPS00188; BIOTIN; 1; .
PROSITEPS50968; BIOTINYL_LIPOYL; 1; .
PROSITEPS50991; PYR_CT; 1; .





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World-2DPAGE Repository (0001):  P52873

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World-2DPAGE Repository (0002):  P52873

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World-2DPAGE Repository (0003):  P52873

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World-2DPAGE Repository (0005):  P52873

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World-2DPAGE Repository (0006):  P52873

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World-2DPAGE Repository (0007):  P52873

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World-2DPAGE Repository (0008):  P52873

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World-2DPAGE Repository (0009):  P52873

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World-2DPAGE Repository (0010):  P52873

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World-2DPAGE Repository (0011):  P52873

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World-2DPAGE Repository (0012):  P52873

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World-2DPAGE Repository (0017):  P52873

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World-2DPAGE Repository (0018):  P52873

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World-2DPAGE Repository (0020):  P52873

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World-2DPAGE Repository (0021):  P52873

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World-2DPAGE Repository (0022):  P52873

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World-2DPAGE Repository (0023):  P52873

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World-2DPAGE Repository (0024):  P52873

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World-2DPAGE Repository (0026):  P52873

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World-2DPAGE Repository (0027):  P52873

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World-2DPAGE Repository (0029):  P52873

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World-2DPAGE Repository (0031):  P52873

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Searching in 'World-2DPAGE Repository [0032]' for entry matching: P52873




World-2DPAGE Repository (0032):  P52873

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World-2DPAGE Repository (0034):  P52873

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World-2DPAGE Repository (0035):  P52873

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World-2DPAGE Repository (0036):  P52873

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World-2DPAGE Repository (0037):  P52873

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World-2DPAGE Repository (0038):  P52873

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World-2DPAGE Repository (0039):  P52873

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World-2DPAGE Repository (0040):  P52873

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World-2DPAGE Repository (0042):  P52873

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World-2DPAGE Repository (0043):  P52873

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World-2DPAGE Repository (0044):  P52873

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World-2DPAGE Repository (0046):  P52873

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World-2DPAGE Repository (0056):  P52873

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World-2DPAGE Repository (0059):  P52873

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World-2DPAGE Repository (0068):  P52873

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World-2DPAGE Repository (0069):  P52873

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World-2DPAGE Repository (0076):  P52873

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World-2DPAGE Repository (0077):  P52873

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World-2DPAGE Repository (0088):  P52873

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World-2DPAGE Repository image

World-2DPAGE Repository (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server