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Searching in 'World-2DPAGE Repository [0030]' for entry matching: P52873

World-2DPAGE Repository (0030):  P52873


General information about the entry
View entry in simple text format
Entry namePYC_RAT
Primary accession numberP52873
integrated into World-2DPAGE Repository (0030) on November 9, 2010 (release 1)
2D Annotations were last modified onJune 21, 2011 (version 2)
General Annotations were last modified on November 23, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Pyruvate carboxylase, mitochondrial; EC=; AltName: Full=Pyruvic carboxylase; Short=PCB; Flags: Precursor;.
Gene nameName=Pc
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C.
''Two-dimensional gel proteome reference map of INS-1E cells''
Proteomics 11(7):1365-1369 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7}
Rattus norvegicus (Rat)
  map experimental info

pI=6.23; Mw=106489  [identification data]
pI=6.48; Mw=100478  [identification data]
pI=6.18; Mw=100062  [identification data]

IDENTIFICATION: SPOT 66: Mascot protein score=186. Number of peptides=6 [1]
SPOT 167: Mascot protein score=30. Number of peptides=3 [1]; SPOT 169: Mascot protein score=42. Number of peptides=5 [1].
MAPPING (identification):
SPOT 66: Tandem mass spectrometry [1];
SPOT 167: Tandem mass spectrometry [1];
SPOT 169: Tandem mass spectrometry [1].

Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium
UniProtKB/Swiss-ProtP52873; PYC_RAT.
World-2DPAGE RepositoryP52873; PYC_RAT.

2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry namePYC_RAT
Primary accession numberP52873
Secondary accession number(s) Q5RKM0 Q64555
Sequence was last modified on May 1, 2007 (version 2)
Annotations were last modified on November 16, 2011 (version 112)
Name and origin of the protein
DescriptionRecName: Full=Pyruvate carboxylase, mitochondrial; EC=; AltName: Full=Pyruvic carboxylase; Short=PCB; Flags: Precursor;
Gene nameName=Pc
Encoded onName=Pc
KeywordsAcetylation; ATP-binding; Biotin; Complete proteome; Direct protein sequencing; Gluconeogenesis; Ligase; Lipid synthesis; Manganese; Metal-binding; Mitochondrion; Multifunctional enzyme; Nucleotide-binding; Phosphoprotein; Pyruvate; Reference proteome; Transit peptide.
Copyrighted by the UniProt Consortium, see Distributed under the Creative Commons Attribution-NoDerivs License
EMBLU32314; AAA96256.1; -; mRNA
EMBLU36585; AAC52668.1; -; mRNA
EMBLBC085680; AAH85680.1; -; mRNA
IPIIPI00210435; -; .
PIRS68252; JC4391; .
RefSeqNP_036876.2; NM_012744.2; .
UniGeneRn.11094; -; .
ProteinModelPortalP52873; -; .
SMRP52873; 35-490; 494-1178; .
MINTMINT-4592965; -; .
STRINGP52873; -; .
World-2DPAGE0004:P52873; -; .
PRIDEP52873; -; .
EnsemblENSRNOT00000026316; ENSRNOP00000026316; ENSRNOG00000019372; .
GeneID25104; -; .
KEGGrno:25104; -; .
NMPDRfig|10116.3.peg.3454; -; .
UCSCNM_012744; rat; .
CTD5091; -; .
RGD3262; Pc; .
eggNOGroNOG05375; -; .
GeneTreeENSGT00550000074986; -; .
HOVERGENHBG008340; -; .
InParanoidP52873; -; .
OrthoDBEOG4ZCT3P; -; .
PhylomeDBP52873; -; .
NextBio605431; -; .
ArrayExpressP52873; -; .
GenevestigatorP52873; -; .
GermOnlineENSRNOG00000019372; Rattus norvegicus; .
GOGO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell; .
GOGO:0005625; C:soluble fraction; IDA:RGD; .
GOGO:0005524; F:ATP binding; IDA:RGD; .
GOGO:0009374; F:biotin binding; IMP:RGD; .
GOGO:0004075; F:biotin carboxylase activity; IEA:InterPro; .
GOGO:0046872; F:metal ion binding; IEA:UniProtKB-KW; .
GOGO:0004736; F:pyruvate carboxylase activity; IDA:RGD; .
GOGO:0006094; P:gluconeogenesis; IDA:RGD; .
GOGO:0008610; P:lipid biosynthetic process; IEA:UniProtKB-KW; .
GOGO:0006107; P:oxaloacetate metabolic process; IDA:RGD; .
GOGO:0006090; P:pyruvate metabolic process; IDA:RGD; .
InterProIPR013785; Aldolase_TIM; .
InterProIPR011761; ATP-grasp; .
InterProIPR013815; ATP_grasp_subdomain_1; .
InterProIPR013816; ATP_grasp_subdomain_2; .
InterProIPR001882; Biotin_BS; .
InterProIPR011764; Biotin_carboxylation_dom; .
InterProIPR005482; Biotin_COase_C; .
InterProIPR000089; Biotin_lipoyl; .
InterProIPR005479; CarbamoylP_synth_lsu_ATP-bd; .
InterProIPR005481; CarbamoylP_synth_lsu_N; .
InterProIPR003379; Carboxylase_cons_dom; .
InterProIPR013817; Pre-ATP_grasp; .
InterProIPR016185; PreATP-grasp-like; .
InterProIPR000891; PYR_CT; .
InterProIPR005930; Pyruv_COase; .
InterProIPR011054; Rudment_hybrid_motif; .
InterProIPR011053; Single_hybrid_motif; .
Gene3DG3DSA:; Aldolase_TIM; 1; .
Gene3DG3DSA:3.30.1490.20; ATP_grasp_subdomain_1; 1; .
Gene3DG3DSA:3.30.470.20; ATP_grasp_subdomain_2; 1; .
Gene3DG3DSA:; Pre-ATP_grasp; 1; .
KOK01958; -; .
PANTHERPTHR18866:SF10; PTHR18866:SF10; 1; .
PfamPF02785; Biotin_carb_C; 1; .
PfamPF00364; Biotin_lipoyl; 1; .
PfamPF00289; CPSase_L_chain; 1; .
PfamPF02786; CPSase_L_D2; 1; .
PfamPF00682; HMGL-like; 1; .
PfamPF02436; PYC_OADA; 1; .
PIRSFPIRSF001594; Pyruv_carbox; 1; .
SMARTSM00878; Biotin_carb_C; 1; .
SUPFAMSSF51230; Hybrid_motif; 1; .
SUPFAMSSF52440; PreATP-grasp-like; 1; .
SUPFAMSSF51246; Rudmnt_hyb_motif; 1; .
TIGRFAMsTIGR01235; Pyruv_carbox; 1; .
PROSITEPS50979; BC; 1; .
PROSITEPS50991; PYR_CT; 1; .

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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server