World-2DPAGE Repository

Entry name

PYGB_RAT

Primary accession number

P53534

integrated into World-2DPAGE Repository (0004) on

October 13, 2008 (release 1)

2D Annotations were last modified on

May 18, 2011 (version 2)

General Annotations were last modified on

December 22, 2011 (version 2)

Name and origin of the protein

Description

RecName: Full=Glycogen phosphorylase, brain form; EC=2.4.1.1; Flags: Fragment;.

Gene name

Name=Pygb

Annotated species

Rattus norvegicus (Rat) [TaxID: 10116]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	MAPPING ON GEL PubMed=19343716; DOI=10.1002/pmic.200800664; [NCBI, EBI, Israel, Japan] Maurya D.K., Sundaram C.S., Bhargava P. ''Proteome profile of the mature rat olfactory bulb'' Proteomics 9(1):2593-2599 (2009)

2D PAGE maps for identified proteins

OB21DSUB {2-DE gel for Olfactory bulb proteome}
Rattus norvegicus (Rat)
Tissue: Olfactory bulb

OB21DSUB

MAP LOCATIONS:

SPOT 138:

pI=6.26; Mw=93000

SPOT 786:

pI=6.35; Mw=94000

MAPPING (identification):

SPOT 138: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 786: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Purnima Bhargava, Centre for Cellular and Molecular Biology, India

Cross-references

UniProtKB/Swiss-Prot

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry name	PYGB_RAT
Primary accession number	P53534
Sequence was last modified on	January 23, 2007 (version 3)
Annotations were last modified on	November 16, 2011 (version 90)
Name and origin of the protein
Description	RecName: Full=Glycogen phosphorylase, brain form; EC=2.4.1.1; Flags: Fragment;
Gene name	Name=Pygb
Encoded on	Name=Pygb
Keywords	Acetylation; Allosteric enzyme; Carbohydrate metabolism; Complete proteome; Direct protein sequencing; Glycogen metabolism; Glycosyltransferase; Phosphoprotein; Pyridoxal phosphate; Reference proteome; Transferase.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	L10668; AAA41252.1; -; mRNA
EMBL	M27726; AAA40815.1; -; mRNA
IPI	IPI00948869; -; .
PIR	S37300; S37300; .
RefSeq	NP_037320.1; NM_013188.1; .
UniGene	Rn.1518; -; .
ProteinModelPortal	P53534; -; .
SMR	P53534; 14-838; .
IntAct	P53534; 1; .
STRING	P53534; -; .
CAZy	GT35; Glycosyltransferase Family 35; .
PhosphoSite	P53534; -; .
World-2DPAGE	0004:P53534; -; .
PRIDE	P53534; -; .
GeneID	25739; -; .
KEGG	rno:25739; -; .
CTD	5834; -; .
RGD	3460; Pygb; .
eggNOG	roNOG12045; -; .
GeneTree	ENSGT00390000016886; -; .
HOVERGEN	HBG006848; -; .
InParanoid	P53534; -; .
PhylomeDB	P53534; -; .
NextBio	607887; -; .
ArrayExpress	P53534; -; .
Genevestigator	P53534; -; .
GermOnline	ENSRNOG00000007583; Rattus norvegicus; .
GO	GO:0030424; C:axon; IDA:RGD; .
GO	GO:0005625; C:soluble fraction; IDA:RGD; .
GO	GO:0008144; F:drug binding; IDA:RGD; .
GO	GO:0008184; F:glycogen phosphorylase activity; IDA:RGD; .
GO	GO:0042803; F:protein homodimerization activity; IDA:RGD; .
GO	GO:0030170; F:pyridoxal phosphate binding; IEA:InterPro; .
GO	GO:0005529; F:sugar binding; IDA:RGD; .
GO	GO:0005980; P:glycogen catabolic process; IDA:RGD; .
InterPro	IPR011833; Glycg_phsphrylas; .
InterPro	IPR000811; Glyco_trans_35; .
KO	K00688; -; .
PANTHER	PTHR11468; Glyco_trans_35; 1; .
Pfam	PF00343; Phosphorylase; 1; .
PIRSF	PIRSF000460; Pprylas_GlgP; 1; .
TIGRFAMs	TIGR02093; P_ylase; 1; .
PROSITE	PS00102; PHOSPHORYLASE; 1; .

Searching in 'World-2DPAGE Repository [0014]' for entry matching: P53534

World-2DPAGE Repository (0014): P53534

P53534

General information about the entry

Entry name

PYGB_RAT

Primary accession number

P53534

integrated into World-2DPAGE Repository (0014) on

October 13, 2008 (release 1)

2D Annotations were last modified on

June 1, 2011 (version 3)

General Annotations were last modified on

November 25, 2011 (version 2)

Name and origin of the protein

Description

RecName: Full=Glycogen phosphorylase, brain form; EC=2.4.1.1; Flags: Fragment;.

Gene name

Name=Pygb

Annotated species

Rattus norvegicus (Rat) [TaxID: 10116]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	2D-PAGE GEL CHARACTERIZATION DOI=10.1002/pmic.200900680; Maurya D.K., Sundaram C.S., Bhargava P. ''Proteome profile of whole cerebellum of the mature rat'' Proteomics 10(23):4311-4319 (2010)

2D PAGE maps for identified proteins

RAT_CEREBELLUM_21D {Proteome of 21 days old rat cerebellum}
Rattus norvegicus (Rat)
Tissue: Cerebellum

RAT_CEREBELLUM_21D

MAP LOCATIONS:

SPOT 3355:

pI=6.36; Mw=79000

IDENTIFICATION: SPOT 3355: SeqCov=36%. MascotPMF=217 [1].
MAPPING (identification):

SPOT 3355: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Purnima Bhargava, Centre for Cellular and Molecular Biology, India

Cross-references

PRIDE

10017.

UniProtKB/Swiss-Prot

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry name	PYGB_RAT
Primary accession number	P53534
Sequence was last modified on	January 23, 2007 (version 3)
Annotations were last modified on	November 16, 2011 (version 90)
Name and origin of the protein
Description	RecName: Full=Glycogen phosphorylase, brain form; EC=2.4.1.1; Flags: Fragment;
Gene name	Name=Pygb
Encoded on	Name=Pygb
Keywords	Acetylation; Allosteric enzyme; Carbohydrate metabolism; Complete proteome; Direct protein sequencing; Glycogen metabolism; Glycosyltransferase; Phosphoprotein; Pyridoxal phosphate; Reference proteome; Transferase.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	L10668; AAA41252.1; -; mRNA
EMBL	M27726; AAA40815.1; -; mRNA
IPI	IPI00948869; -; .
PIR	S37300; S37300; .
RefSeq	NP_037320.1; NM_013188.1; .
UniGene	Rn.1518; -; .
ProteinModelPortal	P53534; -; .
SMR	P53534; 14-838; .
IntAct	P53534; 1; .
STRING	P53534; -; .
CAZy	GT35; Glycosyltransferase Family 35; .
PhosphoSite	P53534; -; .
World-2DPAGE	0004:P53534; -; .
PRIDE	P53534; -; .
GeneID	25739; -; .
KEGG	rno:25739; -; .
CTD	5834; -; .
RGD	3460; Pygb; .
eggNOG	roNOG12045; -; .
GeneTree	ENSGT00390000016886; -; .
HOVERGEN	HBG006848; -; .
InParanoid	P53534; -; .
PhylomeDB	P53534; -; .
NextBio	607887; -; .
ArrayExpress	P53534; -; .
Genevestigator	P53534; -; .
GermOnline	ENSRNOG00000007583; Rattus norvegicus; .
GO	GO:0030424; C:axon; IDA:RGD; .
GO	GO:0005625; C:soluble fraction; IDA:RGD; .
GO	GO:0008144; F:drug binding; IDA:RGD; .
GO	GO:0008184; F:glycogen phosphorylase activity; IDA:RGD; .
GO	GO:0042803; F:protein homodimerization activity; IDA:RGD; .
GO	GO:0030170; F:pyridoxal phosphate binding; IEA:InterPro; .
GO	GO:0005529; F:sugar binding; IDA:RGD; .
GO	GO:0005980; P:glycogen catabolic process; IDA:RGD; .
InterPro	IPR011833; Glycg_phsphrylas; .
InterPro	IPR000811; Glyco_trans_35; .
KO	K00688; -; .
PANTHER	PTHR11468; Glyco_trans_35; 1; .
Pfam	PF00343; Phosphorylase; 1; .
PIRSF	PIRSF000460; Pprylas_GlgP; 1; .
TIGRFAMs	TIGR02093; P_ylase; 1; .
PROSITE	PS00102; PHOSPHORYLASE; 1; .

Searching in 'World-2DPAGE Repository [0019]' for entry matching: P53534

World-2DPAGE Repository (0019): P53534

P53534

General information about the entry

Entry name

PYGB_RAT

Primary accession number

P53534

integrated into World-2DPAGE Repository (0019) on

December 9, 2009 (release 1)

2D Annotations were last modified on

May 30, 2011 (version 2)

General Annotations were last modified on

January 13, 2012 (version 2)

Name and origin of the protein

Description

RecName: Full=Glycogen phosphorylase, brain form; EC=2.4.1.1; Flags: Fragment;.

Gene name

Name=Pygb

Annotated species

Rattus norvegicus (Rat) [TaxID: 10116]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	2D-PAGE GEL CHARACTERIZATION DOI=10.1111/j.1471-4159.2010.06719.x; VanGuilder H.D., Yan H., Farley J.A., Sonntag W.E., Freeman W.M. ''Aging alters the expression of neurotransmission-regulating proteins in the hippocampal synaptoproteome'' J of Neurochemistry 113(6):1577-1588 (2010)

2D PAGE maps for identified proteins

HIPP_SYNAP {Rat hippocampal synaptoproteome}
Rattus norvegicus (Rat)
Tissue: Hippocampus

HIPP_SYNAP

MAP LOCATIONS:

SPOT 182:

pI=6.24; Mw=96854

SPOT 184:

pI=6.24; Mw=96854

SPOT 187:

pI=6.24; Mw=96854

IDENTIFICATION: SPOT 182: Peptides number=8. SeqCov=11.5%. MOWSE score=5.15E+6 [1]
SPOT 184: Peptides number=6. SeqCov=8.5%. MOWSE score=1.24E+5 [1]; SPOT 187: Peptides number=7. SeqCov=11.9%. MOWSE score=5.20E+6 [1].
MAPPING (identification):

SPOT 182: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 184: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 187: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Willard Freeman, Penn State College of Medicine, USA

Cross-references

UniProtKB/Swiss-Prot

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry name	PYGB_RAT
Primary accession number	P53534
Sequence was last modified on	January 23, 2007 (version 3)
Annotations were last modified on	November 16, 2011 (version 90)
Name and origin of the protein
Description	RecName: Full=Glycogen phosphorylase, brain form; EC=2.4.1.1; Flags: Fragment;
Gene name	Name=Pygb
Encoded on	Name=Pygb
Keywords	Acetylation; Allosteric enzyme; Carbohydrate metabolism; Complete proteome; Direct protein sequencing; Glycogen metabolism; Glycosyltransferase; Phosphoprotein; Pyridoxal phosphate; Reference proteome; Transferase.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	L10668; AAA41252.1; -; mRNA
EMBL	M27726; AAA40815.1; -; mRNA
IPI	IPI00948869; -; .
PIR	S37300; S37300; .
RefSeq	NP_037320.1; NM_013188.1; .
UniGene	Rn.1518; -; .
ProteinModelPortal	P53534; -; .
SMR	P53534; 14-838; .
IntAct	P53534; 1; .
STRING	P53534; -; .
CAZy	GT35; Glycosyltransferase Family 35; .
PhosphoSite	P53534; -; .
World-2DPAGE	0004:P53534; -; .
PRIDE	P53534; -; .
GeneID	25739; -; .
KEGG	rno:25739; -; .
CTD	5834; -; .
RGD	3460; Pygb; .
eggNOG	roNOG12045; -; .
GeneTree	ENSGT00390000016886; -; .
HOVERGEN	HBG006848; -; .
InParanoid	P53534; -; .
PhylomeDB	P53534; -; .
NextBio	607887; -; .
ArrayExpress	P53534; -; .
Genevestigator	P53534; -; .
GermOnline	ENSRNOG00000007583; Rattus norvegicus; .
GO	GO:0030424; C:axon; IDA:RGD; .
GO	GO:0005625; C:soluble fraction; IDA:RGD; .
GO	GO:0008144; F:drug binding; IDA:RGD; .
GO	GO:0008184; F:glycogen phosphorylase activity; IDA:RGD; .
GO	GO:0042803; F:protein homodimerization activity; IDA:RGD; .
GO	GO:0030170; F:pyridoxal phosphate binding; IEA:InterPro; .
GO	GO:0005529; F:sugar binding; IDA:RGD; .
GO	GO:0005980; P:glycogen catabolic process; IDA:RGD; .
InterPro	IPR011833; Glycg_phsphrylas; .
InterPro	IPR000811; Glyco_trans_35; .
KO	K00688; -; .
PANTHER	PTHR11468; Glyco_trans_35; 1; .
Pfam	PF00343; Phosphorylase; 1; .
PIRSF	PIRSF000460; Pprylas_GlgP; 1; .
TIGRFAMs	TIGR02093; P_ylase; 1; .
PROSITE	PS00102; PHOSPHORYLASE; 1; .

Searching in 'World-2DPAGE Repository [0022]' for entry matching: P53534

World-2DPAGE Repository (0022): P53534

P53534

General information about the entry

Entry name

PYGB_RAT

Primary accession number

P53534

integrated into World-2DPAGE Repository (0022) on

May 7, 2010 (release 1)

2D Annotations were last modified on

June 22, 2011 (version 2)

General Annotations were last modified on

November 24, 2011 (version 2)

Name and origin of the protein

Description

RecName: Full=Glycogen phosphorylase, brain form; EC=2.4.1.1; Flags: Fragment;.

Gene name

Name=Pygb

Annotated species

Rattus [TaxID: 10114]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae.

References

[1]

2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000593;
Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L.
''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity''
Proteomics 11(16):3369-3379 (2011)

2D PAGE maps for identified proteins

HCR_LCR_HEART {HCR LCR Cardiac Map}
Rattus
Tissue: Heart

HCR_LCR_HEART

MAP LOCATIONS:

SPOT 523:

pI=6.24; Mw=96854

SPOT 728:

pI=6.24; Mw=96854

SPOT 787:

pI=6.24; Mw=96854

SPOT 939:

pI=6.24; Mw=96854

IDENTIFICATION: SPOT 523: SeqCov=19.6%. Peptides MS=14/20. Mascot PMF Score=105 [1]
SPOT 728: SeqCov=20.4%. Peptides MS=14/20. Mascot PMF Score=106 [1]; SPOT 787: SeqCov=20.4%. Peptides MS=15/20. Mascot PMF Score=113 [1]; SPOT 939: SeqCov=19.3%. Peptides MS=14/20. Mascot PMF Score=103 [1].
PEPTIDE EVIDENCE: SPOT 523: 255-LKDFNVGDYIEAVLDR-270 (30) / 294-LKQEYFVVAATLQDIIR-310 (29) [1]
SPOT 728: 51-DYFFALAHTVR-61 (54) / 400-HLEIIYAINQR-410 (55) / 294-LKQEYFVVAATLQDIIR-310 (53) [1]; SPOT 787: 294-LKQEYFVVAATLQDIIR-310 (55) [1]; SPOT 939: 236-NNTVNTMR-243 (1) / 400-HLEIIYAINQR-410 (42) / 774-VFADYEAYIQCQAQVDHLYR-793 (6) [1].
MAPPING (identification):

SPOT 523: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 728: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 787: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 939: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Jatin Burniston, Liverpool John Moores University, UK

Cross-references

UniProtKB/Swiss-Prot