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Searching in 'World-2DPAGE Repository [0030]' for entry matching: P55213




World-2DPAGE Repository (0030):  P55213


P55213


General information about the entry
View entry in simple text format
Entry nameCASP3_RAT
Primary accession numberP55213
integrated into World-2DPAGE Repository (0030) on November 9, 2010 (release 1)
2D Annotations were last modified onJune 21, 2011 (version 2)
General Annotations were last modified on November 23, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Caspase-3; Short=CASP-3; EC=3.4.22.56; AltName: Full=Apopain; AltName: Full=Cysteine protease CPP32; Short=CPP-32; AltName: Full=IRP; AltName: Full=LICE; AltName: Full=Protein Yama; AltName: Full=SREBP cleavage activity 1; Short=SCA-1; Contains: RecName: Full=Caspase-3 subunit p17; Contains: RecName: Full=Caspase-3 subunit p12; Flags: Precursor;.
Gene nameName=Casp3
Synonyms=Cpp32
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000006;
D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C.
''Two-dimensional gel proteome reference map of INS-1E cells''
Proteomics 11(7):1365-1369 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7}
Rattus norvegicus (Rat)
RAT_INS1E_4-7
  map experimental info
 
RAT_INS1E_4-7

MAP LOCATIONS:
pI=6.04; Mw=35504  [identification data]
pI=6.11; Mw=35356  [identification data]

IDENTIFICATION: SPOT 1847: Mascot protein score=63. Number of peptides=3 [1]
SPOT 1860: Mascot protein score=148. Number of peptides=3 [1].
MAPPING (identification):
SPOT 1847: Tandem mass spectrometry [1];
SPOT 1860: Tandem mass spectrometry [1].

Copyright
Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium
Cross-references
UniProtKB/Swiss-ProtP55213; CASP3_RAT.
World-2DPAGE RepositoryP55213; CASP3_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameCASP3_RAT
Primary accession numberP55213
Secondary accession number(s) P70543 P97699 Q62993
Sequence was last modified on November 1, 1997 (version 2)
Annotations were last modified on November 16, 2011 (version 101)
Name and origin of the protein
DescriptionRecName: Full=Caspase-3; Short=CASP-3; EC=3.4.22.56; AltName: Full=Apopain; AltName: Full=Cysteine protease CPP32; Short=CPP-32; AltName: Full=IRP; AltName: Full=LICE; AltName: Full=Protein Yama; AltName: Full=SREBP cleavage activity 1; Short=SCA-1; Contains: RecName: Full=Caspase-3 subunit p17; Contains: RecName: Full=Caspase-3 subunit p12; Flags: Precursor;
Gene nameName=Casp3
Synonyms=Cpp32
Encoded onName=Casp3; Synonyms=Cpp32
KeywordsAcetylation; Apoptosis; Complete proteome; Cytoplasm; Hydrolase; Phosphoprotein; Protease; Reference proteome; S-nitrosylation; Thiol protease; Zymogen.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLU49930; AAC52765.1; -; mRNA
EMBLU84410; AAB41792.1; -; mRNA
EMBLBC081854; AAH81854.1; -; mRNA
EMBLU58656; AAB02722.1; -; mRNA
EMBLU34685; AAC52261.1; -; mRNA
IPIIPI00215220; -; .
PIRI67437; I67437; .
RefSeqNP_037054.1; NM_012922.2; .
UniGeneRn.10562; -; .
ProteinModelPortalP55213; -; .
SMRP55213; 29-277; .
IntActP55213; 1; .
MINTMINT-2635470; -; .
STRINGP55213; -; .
MEROPSC14.003; -; .
PhosphoSiteP55213; -; .
PRIDEP55213; -; .
EnsemblENSRNOT00000014095; ENSRNOP00000014096; ENSRNOG00000010475; .
GeneID25402; -; .
KEGGrno:25402; -; .
NMPDRfig|10116.3.peg.12437; -; .
UCSCNM_012922; rat; .
CTD836; -; .
RGD2275; Casp3; .
eggNOGroNOG08174; -; .
GeneTreeENSGT00560000076782; -; .
HOVERGENHBG050802; -; .
InParanoidP55213; -; .
OMACAMLKQY; -; .
OrthoDBEOG4CZBGR; -; .
PhylomeDBP55213; -; .
BRENDA3.4.22.56; 5301; .
NextBio606497; -; .
ArrayExpressP55213; -; .
GenevestigatorP55213; -; .
GermOnlineENSRNOG00000010475; Rattus norvegicus; .
GOGO:0005829; C:cytosol; IDA:RGD; .
GOGO:0005625; C:soluble fraction; IDA:RGD; .
GOGO:0004197; F:cysteine-type endopeptidase activity; IEA:InterPro; .
GOGO:0016005; F:phospholipase A2 activator activity; IMP:RGD; .
GOGO:0005515; F:protein binding; IPI:RGD; .
GOGO:0034349; P:glial cell apoptosis; IEP:RGD; .
GOGO:0021766; P:hippocampus development; IEP:RGD; .
GOGO:0006917; P:induction of apoptosis; TAS:RGD; .
GOGO:0035556; P:intracellular signal transduction; IMP:RGD; .
GOGO:0007611; P:learning or memory; IMP:RGD; .
GOGO:0030182; P:neuron differentiation; IEP:RGD; .
GOGO:0043525; P:positive regulation of neuron apoptosis; IMP:RGD; .
GOGO:0006508; P:proteolysis; IDA:RGD; .
GOGO:0043200; P:response to amino acid stimulus; IEP:RGD; .
GOGO:0046677; P:response to antibiotic; IEP:RGD; .
GOGO:0032025; P:response to cobalt ion; IEP:RGD; .
GOGO:0042493; P:response to drug; IEP:RGD; .
GOGO:0032355; P:response to estradiol stimulus; IEP:RGD; .
GOGO:0051384; P:response to glucocorticoid stimulus; IEP:RGD; .
GOGO:0009749; P:response to glucose stimulus; IDA:RGD; .
GOGO:0042542; P:response to hydrogen peroxide; IDA:RGD; .
GOGO:0001666; P:response to hypoxia; IEP:RGD; .
GOGO:0032496; P:response to lipopolysaccharide; IEP:RGD; .
GOGO:0035094; P:response to nicotine; IEP:RGD; .
GOGO:0010165; P:response to X-ray; IEP:RGD; .
GOGO:0042060; P:wound healing; IEP:RGD; .
InterProIPR015470; Caspase_3; .
InterProIPR011600; Pept_C14_cat; .
InterProIPR001309; Pept_C14_ICE_p20; .
InterProIPR016129; Pept_C14_ICE_p20_AS; .
InterProIPR002138; Pept_C14_p10; .
InterProIPR002398; Pept_C14_p45; .
InterProIPR015917; Pept_C14_p45_core; .
KOK02187; -; .
PANTHERPTHR10454:SF30; Casp3_like; 1; .
PANTHERPTHR10454; Pept_C14_p45; 1; .
PfamPF00656; Peptidase_C14; 1; .
PRINTSPR00376; IL1BCENZYME; .
SMARTSM00115; CASc; 1; .
PROSITEPS01122; CASPASE_CYS; 1; .
PROSITEPS01121; CASPASE_HIS; 1; .
PROSITEPS50207; CASPASE_P10; 1; .
PROSITEPS50208; CASPASE_P20; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server