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Searching in 'World-2DPAGE Repository [0019]' for entry matching: P59215

World-2DPAGE Repository (0019):  P59215


General information about the entry
View entry in simple text format
Entry nameGNAO_RAT
Primary accession numberP59215
integrated into World-2DPAGE Repository (0019) on December 9, 2009 (release 1)
2D Annotations were last modified onMay 30, 2011 (version 2)
General Annotations were last modified on January 13, 2012 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Guanine nucleotide-binding protein G(o) subunit alpha;.
Gene nameName=Gnao1
Synonyms=Gna0, Gnao
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
VanGuilder H.D., Yan H., Farley J.A., Sonntag W.E., Freeman W.M.
''Aging alters the expression of neurotransmission-regulating proteins in the hippocampal synaptoproteome''
J of Neurochemistry 113(6):1577-1588 (2010)
2D PAGE maps for identified proteins
How to interpret a protein

HIPP_SYNAP {Rat hippocampal synaptoproteome}
Rattus norvegicus (Rat)
Tissue: Hippocampus
  map experimental info

pI=5.34; Mw=40613  [identification data]
pI=5.37; Mw=35959  [identification data]
pI=5.37; Mw=35959  [identification data]

IDENTIFICATION: SPOT 608: Peptides number=7. SeqCov=24.6%. MOWSE score=8.77E+4 [1]
SPOT 1095: Peptides number=7. SeqCov=21.2%. MOWSE score=4.10E+5 [1]; SPOT 1195: Peptides number=7. SeqCov=25.1%. MOWSE score=2.23E+7 [1].
MAPPING (identification):
SPOT 608: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 1095: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 1195: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Data from Dr. Willard Freeman, Penn State College of Medicine, USA
UniProtKB/Swiss-ProtP59215; GNAO_RAT.

2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry nameGNAO_RAT
Primary accession numberP59215
Secondary accession number(s) P04900 P30033
Sequence was last modified on January 23, 2007 (version 2)
Annotations were last modified on December 14, 2011 (version 90)
Name and origin of the protein
DescriptionRecName: Full=Guanine nucleotide-binding protein G(o) subunit alpha;
Gene nameName=Gnao1
Synonyms=Gna0, Gnao
Encoded onName=Gnao1; Synonyms=Gna0, Gnao
KeywordsAlternative splicing; Complete proteome; Direct protein sequencing; GTP-binding; Lipoprotein; Magnesium; Metal-binding; Myristate; Nucleotide-binding; Palmitate; Reference proteome; Transducer.
Copyrighted by the UniProt Consortium, see Distributed under the Creative Commons Attribution-NoDerivs License
EMBLM17526; AAA40826.1; -; mRNA
EMBLM12671; AAA41262.1; -; mRNA
IPIIPI00204843; -; .
IPIIPI00231505; -; .
PIRC40436; RGRTO1; .
PIRD40436; RGRTO2; .
RefSeqNP_059023.1; NM_017327.1; .
UniGeneRn.90161; -; .
ProteinModelPortalP59215; -; .
SMRP59215; 5-353; .
STRINGP59215; -; .
PhosphoSiteP59215; -; .
PRIDEP59215; -; .
EnsemblENSRNOT00000026373; ENSRNOP00000026373; ENSRNOG00000019482; .
GeneID50664; -; .
KEGGrno:50664; -; .
UCSCNM_017327; rat; .
CTD2775; -; .
RGD628732; Gnao1; .
HOVERGENHBG063184; -; .
InParanoidP59215; -; .
OrthoDBEOG44F69B; -; .
PhylomeDBP59215; -; .
NextBio610486; -; .
ArrayExpressP59215; -; .
GenevestigatorP59215; -; .
GermOnlineENSRNOG00000019482; Rattus norvegicus; .
GOGO:0005834; C:heterotrimeric G-protein complex; IBA:RefGenome; .
GOGO:0019717; C:synaptosome; IDA:RGD; .
GOGO:0051430; F:corticotropin-releasing hormone receptor 1 binding; IPI:RGD; .
GOGO:0031683; F:G-protein beta/gamma-subunit complex binding; IBA:RefGenome; .
GOGO:0019003; F:GDP binding; TAS:RGD; .
GOGO:0005525; F:GTP binding; IDA:RGD; .
GOGO:0003924; F:GTPase activity; IBA:RefGenome; .
GOGO:0031821; F:metabotropic serotonin receptor binding; IPI:RGD; .
GOGO:0046872; F:metal ion binding; IEA:UniProtKB-KW; .
GOGO:0031852; F:mu-type opioid receptor binding; IPI:RGD; .
GOGO:0004871; F:signal transducer activity; IBA:RefGenome; .
GOGO:0007568; P:aging; IDA:RGD; .
GOGO:0007212; P:dopamine receptor signaling pathway; IBA:RefGenome; .
GOGO:0030900; P:forebrain development; IEP:RGD; .
GOGO:0007188; P:G-protein signaling; coupled to cAMP nucleotide second messenger; IBA:RefGenome
GOGO:0051926; P:negative regulation of calcium ion transport; IMP:RGD; .
GOGO:0031175; P:neuron projection development; IEP:RGD; .
GOGO:0043547; P:positive regulation of GTPase activity; IMP:RGD; .
GOGO:0034097; P:response to cytokine stimulus; IEP:RGD; .
GOGO:0042493; P:response to drug; IEP:RGD; .
GOGO:0042542; P:response to hydrogen peroxide; IEP:RGD; .
GOGO:0043278; P:response to morphine; IEP:RGD; .
GOGO:0010243; P:response to organic nitrogen; IDA:RGD; .
InterProIPR001408; Gprotein_alpha_I; .
InterProIPR001019; Gprotein_alpha_su; .
InterProIPR011025; GproteinA_insert; .
Gene3DG3DSA:1.10.400.10; GproteinA_insert; 1; .
KOK04534; -; .
PANTHERPTHR10218; Gprotein_alph_bd; 1; .
PfamPF00503; G-alpha; 1; .
SMARTSM00275; G_alpha; 1; .
SUPFAMSSF47895; Transducn_insert; 1; .

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World-2DPAGE Repository (search AC)

Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server