World-2DPAGE Repository

Entry name

CH60_RAT

Primary accession number

P63039

integrated into World-2DPAGE Repository (0004) on

October 13, 2008 (release 1)

2D Annotations were last modified on

May 18, 2011 (version 2)

General Annotations were last modified on

December 22, 2011 (version 2)

Name and origin of the protein

Description

RecName: Full=60 kDa heat shock protein, mitochondrial; AltName: Full=60 kDa chaperonin; AltName: Full=Chaperonin 60; Short=CPN60; AltName: Full=HSP-65; AltName: Full=Heat shock protein 60; Short=HSP-60; Short=Hsp60; AltName: Full=Mitochondrial matrix protein P1; Flags: Precursor;.

Gene name

Name=Hspd1
Synonyms=Hsp60

Annotated species

Rattus norvegicus (Rat) [TaxID: 10116]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	MAPPING ON GEL PubMed=19343716; DOI=10.1002/pmic.200800664; [NCBI, EBI, Israel, Japan] Maurya D.K., Sundaram C.S., Bhargava P. ''Proteome profile of the mature rat olfactory bulb'' Proteomics 9(1):2593-2599 (2009)

2D PAGE maps for identified proteins

OB21DSUB {2-DE gel for Olfactory bulb proteome}
Rattus norvegicus (Rat)
Tissue: Olfactory bulb

OB21DSUB

MAP LOCATIONS:

SPOT 298:

pI=5.53; Mw=56000

MAPPING (identification):

SPOT 298: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Purnima Bhargava, Centre for Cellular and Molecular Biology, India

Cross-references

UniProtKB/Swiss-Prot

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry name	CH60_RAT
Primary accession number	P63039
Secondary accession number(s)	P19226 P19227 P97602
Sequence was last modified on	August 31, 2004 (version 1)
Annotations were last modified on	November 16, 2011 (version 74)
Name and origin of the protein
Description	RecName: Full=60 kDa heat shock protein, mitochondrial; AltName: Full=60 kDa chaperonin; AltName: Full=Chaperonin 60; Short=CPN60; AltName: Full=HSP-65; AltName: Full=Heat shock protein 60; Short=HSP-60; Short=Hsp60; AltName: Full=Mitochondrial matrix protein P1; Flags: Precursor;
Gene name	Name=Hspd1 Synonyms=Hsp60
Encoded on	Name=Hspd1; Synonyms=Hsp60
Keywords	Acetylation; ATP-binding; Chaperone; Complete proteome; Direct protein sequencing; Mitochondrion; Nucleotide-binding; Phosphoprotein; Reference proteome; Transit peptide.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	X54793; CAA38564.1; -; mRNA
EMBL	U68562; AAC53362.1; -; Genomic_DNA
EMBL	BC086507; AAH86507.1; -; mRNA
EMBL	X53585; CAA37654.1; -; mRNA
IPI	IPI00339148; -; .
PIR	S13089; HHRT60; .
RefSeq	NP_071565.2; NM_022229.2; .
UniGene	Rn.102058; -; .
ProteinModelPortal	P63039; -; .
SMR	P63039; 27-554; .
IntAct	P63039; 2; .
MINT	MINT-1860257; -; .
STRING	P63039; -; .
PhosphoSite	P63039; -; .
World-2DPAGE	0004:P63039; -; .
PRIDE	P63039; -; .
Ensembl	ENSRNOT00000019912; ENSRNOP00000019912; ENSRNOG00000014525; .
GeneID	63868; -; .
KEGG	rno:63868; -; .
UCSC	NM_022229; rat; .
CTD	3329; -; .
RGD	621314; Hspd1; .
eggNOG	roNOG14286; -; .
GeneTree	ENSGT00390000005727; -; .
HOVERGEN	HBG001982; -; .
InParanoid	P63039; -; .
OrthoDB	EOG4PK27J; -; .
PhylomeDB	P63039; -; .
NextBio	612488; -; .
Genevestigator	P63039; -; .
GermOnline	ENSRNOG00000014525; Rattus norvegicus; .
GO	GO:0009986; C:cell surface; IDA:RGD; .
GO	GO:0005829; C:cytosol; IDA:RGD; .
GO	GO:0005794; C:Golgi apparatus; IDA:RGD; .
GO	GO:0045121; C:membrane raft; IDA:RGD; .
GO	GO:0030061; C:mitochondrial crista; IDA:RGD; .
GO	GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell; .
GO	GO:0005791; C:rough endoplasmic reticulum; IDA:RGD; .
GO	GO:0042588; C:zymogen granule; IDA:RGD; .
GO	GO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GO	GO:0043559; F:insulin binding; IPI:RGD; .
GO	GO:0051787; F:misfolded protein binding; IPI:RGD; .
GO	GO:0002020; F:protease binding; IPI:RGD; .
GO	GO:0046982; F:protein heterodimerization activity; IPI:RGD; .
GO	GO:0006916; P:anti-apoptosis; IMP:RGD; .
GO	GO:0051085; P:chaperone mediated protein folding requiring cofactor; IDA:RGD; .
GO	GO:0002236; P:detection of misfolded protein; IDA:RGD; .
GO	GO:0045768; P:positive regulation of anti-apoptosis; IMP:RGD; .
GO	GO:0050729; P:positive regulation of inflammatory response; IDA:RGD; .
GO	GO:0042026; P:protein refolding; IEA:InterPro; .
GO	GO:0033198; P:response to ATP; IEP:RGD; .
GO	GO:0042220; P:response to cocaine; IEP:RGD; .
GO	GO:0042493; P:response to drug; IEP:RGD; .
GO	GO:0043627; P:response to estrogen stimulus; IEP:RGD; .
GO	GO:0009408; P:response to heat; IEP:RGD; .
GO	GO:0042542; P:response to hydrogen peroxide; IEP:RGD; .
GO	GO:0001666; P:response to hypoxia; IEP:RGD; .
GO	GO:0032496; P:response to lipopolysaccharide; IEP:RGD; .
InterPro	IPR018370; Chaperonin_Cpn60_CS; .
InterPro	IPR001844; Chaprnin_Cpn60; .
InterPro	IPR002423; Cpn60/TCP-1; .
KO	K04077; -; .
PANTHER	PTHR11353; Cpn60/TCP-1; 1; .
Pfam	PF00118; Cpn60_TCP1; 1; .
PRINTS	PR00298; CHAPERONIN60; .
SUPFAM	SSF48592; GroEL-ATPase; 1; .
TIGRFAMs	TIGR02348; GroEL; 1; .
PROSITE	PS00296; CHAPERONINS_CPN60; 1; .

Searching in 'World-2DPAGE Repository [0014]' for entry matching: P63039

World-2DPAGE Repository (0014): P63039

P63039

General information about the entry

Entry name

CH60_RAT

Primary accession number

P63039

integrated into World-2DPAGE Repository (0014) on

October 13, 2008 (release 1)

2D Annotations were last modified on

June 1, 2011 (version 3)

General Annotations were last modified on

November 25, 2011 (version 2)

Name and origin of the protein

Description

Gene name

Name=Hspd1
Synonyms=Hsp60

Annotated species

Rattus norvegicus (Rat) [TaxID: 10116]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	2D-PAGE GEL CHARACTERIZATION DOI=10.1002/pmic.200900680; Maurya D.K., Sundaram C.S., Bhargava P. ''Proteome profile of whole cerebellum of the mature rat'' Proteomics 10(23):4311-4319 (2010)

2D PAGE maps for identified proteins

RAT_CEREBELLUM_21D {Proteome of 21 days old rat cerebellum}
Rattus norvegicus (Rat)
Tissue: Cerebellum

RAT_CEREBELLUM_21D

MAP LOCATIONS:

SPOT 2821:

pI=5.48; Mw=57000

IDENTIFICATION: SPOT 2821: SeqCov=34%. MascotPMF=123 [1].
MAPPING (identification):

SPOT 2821: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Purnima Bhargava, Centre for Cellular and Molecular Biology, India

Cross-references

PRIDE

10017.

UniProtKB/Swiss-Prot

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry name	CH60_RAT
Primary accession number	P63039
Secondary accession number(s)	P19226 P19227 P97602
Sequence was last modified on	August 31, 2004 (version 1)
Annotations were last modified on	November 16, 2011 (version 74)
Name and origin of the protein
Description	RecName: Full=60 kDa heat shock protein, mitochondrial; AltName: Full=60 kDa chaperonin; AltName: Full=Chaperonin 60; Short=CPN60; AltName: Full=HSP-65; AltName: Full=Heat shock protein 60; Short=HSP-60; Short=Hsp60; AltName: Full=Mitochondrial matrix protein P1; Flags: Precursor;
Gene name	Name=Hspd1 Synonyms=Hsp60
Encoded on	Name=Hspd1; Synonyms=Hsp60
Keywords	Acetylation; ATP-binding; Chaperone; Complete proteome; Direct protein sequencing; Mitochondrion; Nucleotide-binding; Phosphoprotein; Reference proteome; Transit peptide.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	X54793; CAA38564.1; -; mRNA
EMBL	U68562; AAC53362.1; -; Genomic_DNA
EMBL	BC086507; AAH86507.1; -; mRNA
EMBL	X53585; CAA37654.1; -; mRNA
IPI	IPI00339148; -; .
PIR	S13089; HHRT60; .
RefSeq	NP_071565.2; NM_022229.2; .
UniGene	Rn.102058; -; .
ProteinModelPortal	P63039; -; .
SMR	P63039; 27-554; .
IntAct	P63039; 2; .
MINT	MINT-1860257; -; .
STRING	P63039; -; .
PhosphoSite	P63039; -; .
World-2DPAGE	0004:P63039; -; .
PRIDE	P63039; -; .
Ensembl	ENSRNOT00000019912; ENSRNOP00000019912; ENSRNOG00000014525; .
GeneID	63868; -; .
KEGG	rno:63868; -; .
UCSC	NM_022229; rat; .
CTD	3329; -; .
RGD	621314; Hspd1; .
eggNOG	roNOG14286; -; .
GeneTree	ENSGT00390000005727; -; .
HOVERGEN	HBG001982; -; .
InParanoid	P63039; -; .
OrthoDB	EOG4PK27J; -; .
PhylomeDB	P63039; -; .
NextBio	612488; -; .
Genevestigator	P63039; -; .
GermOnline	ENSRNOG00000014525; Rattus norvegicus; .
GO	GO:0009986; C:cell surface; IDA:RGD; .
GO	GO:0005829; C:cytosol; IDA:RGD; .
GO	GO:0005794; C:Golgi apparatus; IDA:RGD; .
GO	GO:0045121; C:membrane raft; IDA:RGD; .
GO	GO:0030061; C:mitochondrial crista; IDA:RGD; .
GO	GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell; .
GO	GO:0005791; C:rough endoplasmic reticulum; IDA:RGD; .
GO	GO:0042588; C:zymogen granule; IDA:RGD; .
GO	GO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GO	GO:0043559; F:insulin binding; IPI:RGD; .
GO	GO:0051787; F:misfolded protein binding; IPI:RGD; .
GO	GO:0002020; F:protease binding; IPI:RGD; .
GO	GO:0046982; F:protein heterodimerization activity; IPI:RGD; .
GO	GO:0006916; P:anti-apoptosis; IMP:RGD; .
GO	GO:0051085; P:chaperone mediated protein folding requiring cofactor; IDA:RGD; .
GO	GO:0002236; P:detection of misfolded protein; IDA:RGD; .
GO	GO:0045768; P:positive regulation of anti-apoptosis; IMP:RGD; .
GO	GO:0050729; P:positive regulation of inflammatory response; IDA:RGD; .
GO	GO:0042026; P:protein refolding; IEA:InterPro; .
GO	GO:0033198; P:response to ATP; IEP:RGD; .
GO	GO:0042220; P:response to cocaine; IEP:RGD; .
GO	GO:0042493; P:response to drug; IEP:RGD; .
GO	GO:0043627; P:response to estrogen stimulus; IEP:RGD; .
GO	GO:0009408; P:response to heat; IEP:RGD; .
GO	GO:0042542; P:response to hydrogen peroxide; IEP:RGD; .
GO	GO:0001666; P:response to hypoxia; IEP:RGD; .
GO	GO:0032496; P:response to lipopolysaccharide; IEP:RGD; .
InterPro	IPR018370; Chaperonin_Cpn60_CS; .
InterPro	IPR001844; Chaprnin_Cpn60; .
InterPro	IPR002423; Cpn60/TCP-1; .
KO	K04077; -; .
PANTHER	PTHR11353; Cpn60/TCP-1; 1; .
Pfam	PF00118; Cpn60_TCP1; 1; .
PRINTS	PR00298; CHAPERONIN60; .
SUPFAM	SSF48592; GroEL-ATPase; 1; .
TIGRFAMs	TIGR02348; GroEL; 1; .
PROSITE	PS00296; CHAPERONINS_CPN60; 1; .

Searching in 'World-2DPAGE Repository [0019]' for entry matching: P63039

World-2DPAGE Repository (0019): P63039

P63039

General information about the entry

Entry name

CH60_RAT

Primary accession number

P63039

integrated into World-2DPAGE Repository (0019) on

December 9, 2009 (release 1)

2D Annotations were last modified on

May 30, 2011 (version 2)

General Annotations were last modified on

January 13, 2012 (version 2)

Name and origin of the protein

Description

Gene name

Name=Hspd1
Synonyms=Hsp60

Annotated species

Rattus norvegicus (Rat) [TaxID: 10116]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	2D-PAGE GEL CHARACTERIZATION DOI=10.1111/j.1471-4159.2010.06719.x; VanGuilder H.D., Yan H., Farley J.A., Sonntag W.E., Freeman W.M. ''Aging alters the expression of neurotransmission-regulating proteins in the hippocampal synaptoproteome'' J of Neurochemistry 113(6):1577-1588 (2010)

2D PAGE maps for identified proteins

HIPP_SYNAP {Rat hippocampal synaptoproteome}
Rattus norvegicus (Rat)
Tissue: Hippocampus

HIPP_SYNAP

MAP LOCATIONS:

SPOT 381:

pI=5.91; Mw=61098

SPOT 383:

pI=5.91; Mw=61098

SPOT 386:

pI=5.91; Mw=61098

SPOT 387:

pI=5.91; Mw=61098

SPOT 645:

pI=5.91; Mw=61098

IDENTIFICATION: SPOT 381: Peptides number=9. SeqCov=25%. MOWSE score=2.00E+13 [1]
SPOT 383: Peptides number=9. SeqCov=18%. MOWSE score=2.02E+9 [1]; SPOT 386: Peptides number=12. SeqCov=18%. MOWSE score=2.27E+5 [1]; SPOT 387: Peptides number=11. SeqCov=23.2%. MOWSE score=2.83E+11 [1]; SPOT 645: Peptides number=3. SeqCov=7.5%. MOWSE score=4.38E+3 [1].
MAPPING (identification):

SPOT 381: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 383: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 386: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 387: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 645: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Willard Freeman, Penn State College of Medicine, USA

Cross-references

UniProtKB/Swiss-Prot

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry name	CH60_RAT
Primary accession number	P63039
Secondary accession number(s)	P19226 P19227 P97602
Sequence was last modified on	August 31, 2004 (version 1)
Annotations were last modified on	November 16, 2011 (version 74)
Name and origin of the protein
Description	RecName: Full=60 kDa heat shock protein, mitochondrial; AltName: Full=60 kDa chaperonin; AltName: Full=Chaperonin 60; Short=CPN60; AltName: Full=HSP-65; AltName: Full=Heat shock protein 60; Short=HSP-60; Short=Hsp60; AltName: Full=Mitochondrial matrix protein P1; Flags: Precursor;
Gene name	Name=Hspd1 Synonyms=Hsp60
Encoded on	Name=Hspd1; Synonyms=Hsp60
Keywords	Acetylation; ATP-binding; Chaperone; Complete proteome; Direct protein sequencing; Mitochondrion; Nucleotide-binding; Phosphoprotein; Reference proteome; Transit peptide.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	X54793; CAA38564.1; -; mRNA
EMBL	U68562; AAC53362.1; -; Genomic_DNA
EMBL	BC086507; AAH86507.1; -; mRNA
EMBL	X53585; CAA37654.1; -; mRNA
IPI	IPI00339148; -; .
PIR	S13089; HHRT60; .
RefSeq	NP_071565.2; NM_022229.2; .
UniGene	Rn.102058; -; .
ProteinModelPortal	P63039; -; .
SMR	P63039; 27-554; .
IntAct	P63039; 2; .
MINT	MINT-1860257; -; .
STRING	P63039; -; .
PhosphoSite	P63039; -; .
World-2DPAGE	0004:P63039; -; .
PRIDE	P63039; -; .
Ensembl	ENSRNOT00000019912; ENSRNOP00000019912; ENSRNOG00000014525; .
GeneID	63868; -; .
KEGG	rno:63868; -; .
UCSC	NM_022229; rat; .
CTD	3329; -; .
RGD	621314; Hspd1; .
eggNOG	roNOG14286; -; .
GeneTree	ENSGT00390000005727; -; .
HOVERGEN	HBG001982; -; .
InParanoid	P63039; -; .
OrthoDB	EOG4PK27J; -; .
PhylomeDB	P63039; -; .
NextBio	612488; -; .
Genevestigator	P63039; -; .
GermOnline	ENSRNOG00000014525; Rattus norvegicus; .
GO	GO:0009986; C:cell surface; IDA:RGD; .
GO	GO:0005829; C:cytosol; IDA:RGD; .
GO	GO:0005794; C:Golgi apparatus; IDA:RGD; .
GO	GO:0045121; C:membrane raft; IDA:RGD; .
GO	GO:0030061; C:mitochondrial crista; IDA:RGD; .
GO	GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell; .
GO	GO:0005791; C:rough endoplasmic reticulum; IDA:RGD; .
GO	GO:0042588; C:zymogen granule; IDA:RGD; .
GO	GO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GO	GO:0043559; F:insulin binding; IPI:RGD; .
GO	GO:0051787; F:misfolded protein binding; IPI:RGD; .
GO	GO:0002020; F:protease binding; IPI:RGD; .
GO	GO:0046982; F:protein heterodimerization activity; IPI:RGD; .
GO	GO:0006916; P:anti-apoptosis; IMP:RGD; .
GO	GO:0051085; P:chaperone mediated protein folding requiring cofactor; IDA:RGD; .
GO	GO:0002236; P:detection of misfolded protein; IDA:RGD; .
GO	GO:0045768; P:positive regulation of anti-apoptosis; IMP:RGD; .
GO	GO:0050729; P:positive regulation of inflammatory response; IDA:RGD; .
GO	GO:0042026; P:protein refolding; IEA:InterPro; .
GO	GO:0033198; P:response to ATP; IEP:RGD; .
GO	GO:0042220; P:response to cocaine; IEP:RGD; .
GO	GO:0042493; P:response to drug; IEP:RGD; .
GO	GO:0043627; P:response to estrogen stimulus; IEP:RGD; .
GO	GO:0009408; P:response to heat; IEP:RGD; .
GO	GO:0042542; P:response to hydrogen peroxide; IEP:RGD; .
GO	GO:0001666; P:response to hypoxia; IEP:RGD; .
GO	GO:0032496; P:response to lipopolysaccharide; IEP:RGD; .
InterPro	IPR018370; Chaperonin_Cpn60_CS; .
InterPro	IPR001844; Chaprnin_Cpn60; .
InterPro	IPR002423; Cpn60/TCP-1; .
KO	K04077; -; .
PANTHER	PTHR11353; Cpn60/TCP-1; 1; .
Pfam	PF00118; Cpn60_TCP1; 1; .
PRINTS	PR00298; CHAPERONIN60; .
SUPFAM	SSF48592; GroEL-ATPase; 1; .
TIGRFAMs	TIGR02348; GroEL; 1; .
PROSITE	PS00296; CHAPERONINS_CPN60; 1; .

Searching in 'World-2DPAGE Repository [0022]' for entry matching: P63039

World-2DPAGE Repository (0022): P63039

P63039

General information about the entry

Entry name

CH60_RAT

Primary accession number

P63039

integrated into World-2DPAGE Repository (0022) on

May 7, 2010 (release 1)

2D Annotations were last modified on

June 22, 2011 (version 2)

General Annotations were last modified on

November 24, 2011 (version 2)

Name and origin of the protein

Description

Gene name

Name=Hspd1
Synonyms=Hsp60

Annotated species

Rattus [TaxID: 10114]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae.

References

[1]

2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000593;
Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L.
''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity''
Proteomics 11(16):3369-3379 (2011)

2D PAGE maps for identified proteins

HCR_LCR_HEART {HCR LCR Cardiac Map}
Rattus
Tissue: Heart

HCR_LCR_HEART

MAP LOCATIONS:

SPOT 351:

pI=5.91; Mw=61088

SPOT 420:

pI=5.91; Mw=61088

SPOT 510:

pI=5.91; Mw=61088

SPOT 792:

pI=5.91; Mw=61088

IDENTIFICATION: SPOT 351: SeqCov=25.8%. Peptides MS=11/20. Mascot PMF Score=88 [1]
SPOT 420: SeqCov=31.8%. Peptides MS=17/20. Mascot PMF Score=169 [1]; SPOT 510: SeqCov=31.4%. Peptides MS=16/20. Mascot PMF Score=144 [1]; SPOT 792: SeqCov=22.9%. Peptides MS=11/20. Mascot PMF Score=84 [1].
PEPTIDE EVIDENCE: SPOT 351: 269-KPLVIIAEDVDGEALSTLVLNR-290 (76) [1]
SPOT 792: 430-AAVEEGIVLGGGCALLR-446 (66) / 269-KPLVIIAEDVDGEALSTLVLNR-290 (89) [1].
MAPPING (identification):

SPOT 351: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 420: Peptide mass fingerprinting [1];
SPOT 510: Peptide mass fingerprinting [1];
SPOT 792: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Jatin Burniston, Liverpool John Moores University, UK

Cross-references

UniProtKB/Swiss-Prot

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry name	CH60_RAT
Primary accession number	P63039
Secondary accession number(s)	P19226 P19227 P97602
Sequence was last modified on	August 31, 2004 (version 1)
Annotations were last modified on	November 16, 2011 (version 74)
Name and origin of the protein
Description	RecName: Full=60 kDa heat shock protein, mitochondrial; AltName: Full=60 kDa chaperonin; AltName: Full=Chaperonin 60; Short=CPN60; AltName: Full=HSP-65; AltName: Full=Heat shock protein 60; Short=HSP-60; Short=Hsp60; AltName: Full=Mitochondrial matrix protein P1; Flags: Precursor;
Gene name	Name=Hspd1 Synonyms=Hsp60
Encoded on	Name=Hspd1; Synonyms=Hsp60
Keywords	Acetylation; ATP-binding; Chaperone; Complete proteome; Direct protein sequencing; Mitochondrion; Nucleotide-binding; Phosphoprotein; Reference proteome; Transit peptide.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	X54793; CAA38564.1; -; mRNA
EMBL	U68562; AAC53362.1; -; Genomic_DNA
EMBL	BC086507; AAH86507.1; -; mRNA
EMBL	X53585; CAA37654.1; -; mRNA
IPI	IPI00339148; -; .
PIR	S13089; HHRT60; .
RefSeq	NP_071565.2; NM_022229.2; .
UniGene	Rn.102058; -; .
ProteinModelPortal	P63039; -; .
SMR	P63039; 27-554; .
IntAct	P63039; 2; .
MINT	MINT-1860257; -; .
STRING	P63039; -; .
PhosphoSite	P63039; -; .
World-2DPAGE	0004:P63039; -; .
PRIDE	P63039; -; .
Ensembl	ENSRNOT00000019912; ENSRNOP00000019912; ENSRNOG00000014525; .
GeneID	63868; -; .
KEGG	rno:63868; -; .
UCSC	NM_022229; rat; .
CTD	3329; -; .
RGD	621314; Hspd1; .
eggNOG	roNOG14286; -; .
GeneTree	ENSGT00390000005727; -; .
HOVERGEN	HBG001982; -; .
InParanoid	P63039; -; .
OrthoDB	EOG4PK27J; -; .
PhylomeDB	P63039; -; .
NextBio	612488; -; .
Genevestigator	P63039; -; .
GermOnline	ENSRNOG00000014525; Rattus norvegicus; .
GO	GO:0009986; C:cell surface; IDA:RGD; .
GO	GO:0005829; C:cytosol; IDA:RGD; .
GO	GO:0005794; C:Golgi apparatus; IDA:RGD; .
GO	GO:0045121; C:membrane raft; IDA:RGD; .
GO	GO:0030061; C:mitochondrial crista; IDA:RGD; .
GO	GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell; .
GO	GO:0005791; C:rough endoplasmic reticulum; IDA:RGD; .
GO	GO:0042588; C:zymogen granule; IDA:RGD; .
GO	GO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GO	GO:0043559; F:insulin binding; IPI:RGD; .
GO	GO:0051787; F:misfolded protein binding; IPI:RGD; .
GO	GO:0002020; F:protease binding; IPI:RGD; .
GO	GO:0046982; F:protein heterodimerization activity; IPI:RGD; .
GO	GO:0006916; P:anti-apoptosis; IMP:RGD; .
GO	GO:0051085; P:chaperone mediated protein folding requiring cofactor; IDA:RGD; .
GO	GO:0002236; P:detection of misfolded protein; IDA:RGD; .
GO	GO:0045768; P:positive regulation of anti-apoptosis; IMP:RGD; .
GO	GO:0050729; P:positive regulation of inflammatory response; IDA:RGD; .
GO	GO:0042026; P:protein refolding; IEA:InterPro; .
GO	GO:0033198; P:response to ATP; IEP:RGD; .
GO	GO:0042220; P:response to cocaine; IEP:RGD; .
GO	GO:0042493; P:response to drug; IEP:RGD; .
GO	GO:0043627; P:response to estrogen stimulus; IEP:RGD; .
GO	GO:0009408; P:response to heat; IEP:RGD; .
GO	GO:0042542; P:response to hydrogen peroxide; IEP:RGD; .
GO	GO:0001666; P:response to hypoxia; IEP:RGD; .
GO	GO:0032496; P:response to lipopolysaccharide; IEP:RGD; .
InterPro	IPR018370; Chaperonin_Cpn60_CS; .
InterPro	IPR001844; Chaprnin_Cpn60; .
InterPro	IPR002423; Cpn60/TCP-1; .
KO	K04077; -; .
PANTHER	PTHR11353; Cpn60/TCP-1; 1; .
Pfam	PF00118; Cpn60_TCP1; 1; .
PRINTS	PR00298; CHAPERONIN60; .
SUPFAM	SSF48592; GroEL-ATPase; 1; .
TIGRFAMs	TIGR02348; GroEL; 1; .
PROSITE	PS00296; CHAPERONINS_CPN60; 1; .

Searching in 'World-2DPAGE Repository [0030]' for entry matching: P63039

World-2DPAGE Repository (0030): P63039

P63039

General information about the entry

Entry name

CH60_RAT

Primary accession number

P63039

integrated into World-2DPAGE Repository (0030) on

November 9, 2010 (release 1)

2D Annotations were last modified on

June 21, 2011 (version 2)

General Annotations were last modified on

November 23, 2011 (version 2)

Name and origin of the protein

Description

Gene name

Name=Hspd1
Synonyms=Hsp60

Annotated species

Rattus norvegicus (Rat) [TaxID: 10116]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	2D-PAGE GEL CHARACTERIZATION DOI=10.1002/pmic.201000006; D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C. ''Two-dimensional gel proteome reference map of INS-1E cells'' Proteomics 11(7):1365-1369 (2011)

2D PAGE maps for identified proteins

RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7}
Rattus norvegicus (Rat)

RAT_INS1E_4-7

MAP LOCATIONS:

SPOT 783:

pI=5.35; Mw=70221

SPOT 803:

pI=5.28; Mw=69065

SPOT 809:

pI=5.36; Mw=68589

SPOT 2051:

pI=4.49; Mw=30282

IDENTIFICATION: SPOT 783: Mascot protein score=117. Number of peptides=5 [1]
SPOT 803: Mascot protein score=289. Number of peptides=5 [1]; SPOT 809: Mascot protein score=219. Number of peptides=5 [1]; SPOT 2051: Mascot protein score=205. Number of peptides=5 [1].
MAPPING (identification):

SPOT 783: Tandem mass spectrometry [1];
SPOT 803: Tandem mass spectrometry [1];
SPOT 809: Tandem mass spectrometry [1];
SPOT 2051: Tandem mass spectrometry [1].

Copyright

Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium

Cross-references

UCD-2DPAGE

P19227; CH60_RAT.

UniProtKB/Swiss-Prot

World-2DPAGE Repository