World-2DPAGE Home
Home  |  Contact
Due to scheduled maintenance work, this service will not be available from Tuesday August 23rd 06:00 pm until Wednesday August 24th 08:00 am CEST. Apologies for the inconvenience.

World-2DPAGE Repository 
Search by  [accession number] *
[description, ID or gene] 
[author names] 
[spot ID / serial number] 
[identification methods] 
[pI / Mw range] 
[combined fields] 
Maps  [experimental info] 
[protein list] 
[graphical interface]
     Databases

use 'Ctrl' to select several
databases

Select Remote Interfaces
[All Interfaces]
SWISS-2DPAGE
World-2DPAGE Portal
Exclude local DBs
has only effect if a remote
interface is selected
     
Searching in 'World-2DPAGE Repository [0022]' for entry matching: P63039




World-2DPAGE Repository (0022):  P63039

P63039


General information about the entry
View entry in simple text format
Entry nameCH60_RAT
Primary accession numberP63039
integrated into World-2DPAGE Repository (0022) on May 7, 2010 (release 1)
2D Annotations were last modified onJune 22, 2011 (version 2)
General Annotations were last modified on November 24, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=60 kDa heat shock protein, mitochondrial; AltName: Full=60 kDa chaperonin; AltName: Full=Chaperonin 60; Short=CPN60; AltName: Full=HSP-65; AltName: Full=Heat shock protein 60; Short=HSP-60; Short=Hsp60; AltName: Full=Mitochondrial matrix protein P1; Flags: Precursor;.
Gene nameName=Hspd1
Synonyms=Hsp60
Annotated speciesRattus [TaxID: 10114]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000593;
Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L.
''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity''
Proteomics 11(16):3369-3379 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

HCR_LCR_HEART {HCR LCR Cardiac Map}
Rattus
Tissue: Heart
HCR_LCR_HEART
  map experimental info 		 
HCR_LCR_HEART

MAP LOCATIONS:
pI=5.91; Mw=61088  [identification data]
pI=5.91; Mw=61088  [identification data]
pI=5.91; Mw=61088  [identification data]
pI=5.91; Mw=61088  [identification data]

IDENTIFICATION: SPOT 351: SeqCov=25.8%. Peptides MS=11/20. Mascot PMF Score=88 [1]
SPOT 420: SeqCov=31.8%. Peptides MS=17/20. Mascot PMF Score=169 [1]; SPOT 510: SeqCov=31.4%. Peptides MS=16/20. Mascot PMF Score=144 [1]; SPOT 792: SeqCov=22.9%. Peptides MS=11/20. Mascot PMF Score=84 [1].
PEPTIDE EVIDENCE: SPOT 351: 269-KPLVIIAEDVDGEALSTLVLNR-290 (76) [1]
SPOT 792: 430-AAVEEGIVLGGGCALLR-446 (66) / 269-KPLVIIAEDVDGEALSTLVLNR-290 (89) [1].
MAPPING (identification):
SPOT 351: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 420: Peptide mass fingerprinting [1];
SPOT 510: Peptide mass fingerprinting [1];
SPOT 792: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright
Data from Dr. Jatin Burniston, Liverpool John Moores University, UK
Cross-references
UniProtKB/Swiss-ProtP63039; CH60_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameCH60_RAT
Primary accession numberP63039
Secondary accession number(s) P19226 P19227 P97602
Sequence was last modified on August 31, 2004 (version 1)
Annotations were last modified on November 16, 2011 (version 74)
Name and origin of the protein
DescriptionRecName: Full=60 kDa heat shock protein, mitochondrial; AltName: Full=60 kDa chaperonin; AltName: Full=Chaperonin 60; Short=CPN60; AltName: Full=HSP-65; AltName: Full=Heat shock protein 60; Short=HSP-60; Short=Hsp60; AltName: Full=Mitochondrial matrix protein P1; Flags: Precursor;
Gene nameName=Hspd1
Synonyms=Hsp60
Encoded onName=Hspd1; Synonyms=Hsp60
KeywordsAcetylation; ATP-binding; Chaperone; Complete proteome; Direct protein sequencing; Mitochondrion; Nucleotide-binding; Phosphoprotein; Reference proteome; Transit peptide.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLX54793; CAA38564.1; -; mRNA
EMBLU68562; AAC53362.1; -; Genomic_DNA
EMBLBC086507; AAH86507.1; -; mRNA
EMBLX53585; CAA37654.1; -; mRNA
IPIIPI00339148; -; .
PIRS13089; HHRT60; .
RefSeqNP_071565.2; NM_022229.2; .
UniGeneRn.102058; -; .
ProteinModelPortalP63039; -; .
SMRP63039; 27-554; .
IntActP63039; 2; .
MINTMINT-1860257; -; .
STRINGP63039; -; .
PhosphoSiteP63039; -; .
World-2DPAGE0004:P63039; -; .
PRIDEP63039; -; .
EnsemblENSRNOT00000019912; ENSRNOP00000019912; ENSRNOG00000014525; .
GeneID63868; -; .
KEGGrno:63868; -; .
UCSCNM_022229; rat; .
CTD3329; -; .
RGD621314; Hspd1; .
eggNOGroNOG14286; -; .
GeneTreeENSGT00390000005727; -; .
HOVERGENHBG001982; -; .
InParanoidP63039; -; .
OrthoDBEOG4PK27J; -; .
PhylomeDBP63039; -; .
NextBio612488; -; .
GenevestigatorP63039; -; .
GermOnlineENSRNOG00000014525; Rattus norvegicus; .
GOGO:0009986; C:cell surface; IDA:RGD; .
GOGO:0005829; C:cytosol; IDA:RGD; .
GOGO:0005794; C:Golgi apparatus; IDA:RGD; .
GOGO:0045121; C:membrane raft; IDA:RGD; .
GOGO:0030061; C:mitochondrial crista; IDA:RGD; .
GOGO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell; .
GOGO:0005791; C:rough endoplasmic reticulum; IDA:RGD; .
GOGO:0042588; C:zymogen granule; IDA:RGD; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0043559; F:insulin binding; IPI:RGD; .
GOGO:0051787; F:misfolded protein binding; IPI:RGD; .
GOGO:0002020; F:protease binding; IPI:RGD; .
GOGO:0046982; F:protein heterodimerization activity; IPI:RGD; .
GOGO:0006916; P:anti-apoptosis; IMP:RGD; .
GOGO:0051085; P:chaperone mediated protein folding requiring cofactor; IDA:RGD; .
GOGO:0002236; P:detection of misfolded protein; IDA:RGD; .
GOGO:0045768; P:positive regulation of anti-apoptosis; IMP:RGD; .
GOGO:0050729; P:positive regulation of inflammatory response; IDA:RGD; .
GOGO:0042026; P:protein refolding; IEA:InterPro; .
GOGO:0033198; P:response to ATP; IEP:RGD; .
GOGO:0042220; P:response to cocaine; IEP:RGD; .
GOGO:0042493; P:response to drug; IEP:RGD; .
GOGO:0043627; P:response to estrogen stimulus; IEP:RGD; .
GOGO:0009408; P:response to heat; IEP:RGD; .
GOGO:0042542; P:response to hydrogen peroxide; IEP:RGD; .
GOGO:0001666; P:response to hypoxia; IEP:RGD; .
GOGO:0032496; P:response to lipopolysaccharide; IEP:RGD; .
InterProIPR018370; Chaperonin_Cpn60_CS; .
InterProIPR001844; Chaprnin_Cpn60; .
InterProIPR002423; Cpn60/TCP-1; .
KOK04077; -; .
PANTHERPTHR11353; Cpn60/TCP-1; 1; .
PfamPF00118; Cpn60_TCP1; 1; .
PRINTSPR00298; CHAPERONIN60; .
SUPFAMSSF48592; GroEL-ATPase; 1; .
TIGRFAMsTIGR02348; GroEL; 1; .
PROSITEPS00296; CHAPERONINS_CPN60; 1; .


World-2DPAGE Repository image

World-2DPAGE Repository (search AC)

Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server