World-2DPAGE Repository

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	MAPPING ON GEL PubMed=19343716; DOI=10.1002/pmic.200800664; [NCBI, EBI, Israel, Japan] Maurya D.K., Sundaram C.S., Bhargava P. ''Proteome profile of the mature rat olfactory bulb'' Proteomics 9(1):2593-2599 (2009)

2D PAGE maps for identified proteins

How to interpret a protein

OB21DSUB {2-DE gel for Olfactory bulb proteome}
Rattus norvegicus (Rat)
Tissue: Olfactory bulb

map experimental info

OB21DSUB

MAP LOCATIONS:

SPOT 325:

pI=6.23; Mw=52000

[identification data]

SPOT 327:

pI=6.35; Mw=52000

[identification data]

MAPPING (identification):

SPOT 325: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 327: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Purnima Bhargava, Centre for Cellular and Molecular Biology, India

Cross-references

UniProtKB/Swiss-Prot

P85845; FSCN1_RAT.

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry name	FSCN1_RAT
Primary accession number	P85845
Secondary accession number(s)	B5DEI1
Sequence was last modified on	September 22, 2009 (version 2)
Annotations were last modified on	October 19, 2011 (version 22)
Name and origin of the protein
Description	RecName: Full=Fascin;
Gene name	Name=Fscn1
Encoded on	Name=Fscn1
Keywords	Acetylation; Actin-binding; Cell junction; Cell projection; Complete proteome; Cytoplasm; Cytoskeleton; Phosphoprotein; Reference proteome.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	BC168678; AAI68678.1; -; mRNA
IPI	IPI00896761; -; .
RefSeq	NP_001094276.1; NM_001100806.1; .
UniGene	Rn.199526; -; .
ProteinModelPortal	P85845; -; .
World-2DPAGE	0004:P85845; -; .
PRIDE	P85845; -; .
GeneID	683788; -; .
KEGG	rno:683788; -; .
HOVERGEN	HBG000968; -; .
Genevestigator	P85845; -; .
GO	GO:0030054; C:cell junction; IEA:UniProtKB-KW; .
GO	GO:0005737; C:cytoplasm; IDA:RGD; .
GO	GO:0030425; C:dendrite; IDA:RGD; .
GO	GO:0030175; C:filopodium; IDA:RGD; .
GO	GO:0030426; C:growth cone; IDA:RGD; .
GO	GO:0071437; C:invadopodium; ISS:UniProtKB; .
GO	GO:0030027; C:lamellipodium; IDA:RGD; .
GO	GO:0005902; C:microvillus; IDA:RGD; .
GO	GO:0005886; C:plasma membrane; IDA:RGD; .
GO	GO:0001726; C:ruffle; IDA:RGD; .
GO	GO:0001725; C:stress fiber; IDA:RGD; .
GO	GO:0051015; F:actin filament binding; IEA:InterPro; .
GO	GO:0030674; F:protein binding; bridging; IEA:InterPro
GO	GO:0051017; P:actin filament bundle assembly; IDA:UniProtKB; .
GO	GO:0048870; P:cell motility; ISS:UniProtKB; .
GO	GO:0071460; P:cellular response to cell-matrix adhesion; IEP:RGD; .
GO	GO:0001889; P:liver development; IEP:RGD; .
InterPro	IPR008999; Actin_cross-linking; .
InterPro	IPR024703; Fascin; .
InterPro	IPR022768; Fascin-domain; .
Pfam	PF06268; Fascin; 4; .
PIRSF	PIRSF005682; Fascin; 1; .
SUPFAM	SSF50405; Actin_crosslink; 4; .

World-2DPAGE Repository (search AC)

Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server

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