World-2DPAGE Repository

Entry name

VINC_RAT

Primary accession number

P85972

integrated into World-2DPAGE Repository (0004) on

October 13, 2008 (release 1)

2D Annotations were last modified on

May 18, 2011 (version 2)

General Annotations were last modified on

December 22, 2011 (version 2)

Name and origin of the protein

Description

RecName: Full=Vinculin; AltName: Full=Metavinculin;.

Gene name

Name=Vcl

Annotated species

Rattus norvegicus (Rat) [TaxID: 10116]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	MAPPING ON GEL PubMed=19343716; DOI=10.1002/pmic.200800664; [NCBI, EBI, Israel, Japan] Maurya D.K., Sundaram C.S., Bhargava P. ''Proteome profile of the mature rat olfactory bulb'' Proteomics 9(1):2593-2599 (2009)

2D PAGE maps for identified proteins

OB21DSUB {2-DE gel for Olfactory bulb proteome}
Rattus norvegicus (Rat)
Tissue: Olfactory bulb

OB21DSUB

MAP LOCATIONS:

SPOT 80:

pI=5.91; Mw=95000

MAPPING (identification):

SPOT 80: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Purnima Bhargava, Centre for Cellular and Molecular Biology, India

Cross-references

UniProtKB/Swiss-Prot

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry name	VINC_RAT
Primary accession number	P85972
Secondary accession number(s)	A6KKR4
Sequence was last modified on	September 2, 2008 (version 1)
Annotations were last modified on	December 14, 2011 (version 27)
Name and origin of the protein
Description	RecName: Full=Vinculin; AltName: Full=Metavinculin;
Gene name	Name=Vcl
Encoded on	Name=Vcl
Keywords	Acetylation; Actin-binding; Cell adhesion; Cell junction; Cell membrane; Complete proteome; Cytoplasm; Cytoskeleton; Lipoprotein; Membrane; Myristate; Palmitate; Phosphoprotein; Reference proteome; Repeat.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	CH474061; EDL86258.1; -; Genomic_DNA
IPI	IPI00365286; -; .
RefSeq	NP_001100718.1; NM_001107248.1; .
UniGene	Rn.164613; -; .
MINT	MINT-6804299; -; .
STRING	P85972; -; .
PhosphoSite	P85972; -; .
World-2DPAGE	0004:P85972; -; .
PRIDE	P85972; -; .
Ensembl	ENSRNOT00000015179; ENSRNOP00000015179; ENSRNOG00000010765; .
GeneID	305679; -; .
KEGG	rno:305679; -; .
CTD	7414; -; .
RGD	1311217; Vcl; .
eggNOG	roNOG04310; -; .
GeneTree	ENSGT00550000074411; -; .
HOVERGEN	HBG079758; -; .
OMA	ELTHQVH; -; .
OrthoDB	EOG4P5K8C; -; .
PhylomeDB	P85972; -; .
Genevestigator	P85972; -; .
GO	GO:0015629; C:actin cytoskeleton; IEA:InterPro; .
GO	GO:0003779; F:actin binding; IEA:UniProtKB-KW; .
GO	GO:0017048; F:Rho GTPase binding; IPI:RGD; .
GO	GO:0005198; F:structural molecule activity; IEA:InterPro; .
InterPro	IPR017997; Vinculin; .
InterPro	IPR006077; Vinculin/catenin; .
InterPro	IPR000633; Vinculin_CS; .
KO	K05700; -; .
Pfam	PF01044; Vinculin; 2; .
PRINTS	PR00806; VINCULIN; .
SUPFAM	SSF47220; Vinculin/catenin; 7; .
PROSITE	PS00663; VINCULIN_1; 1; .
PROSITE	PS00664; VINCULIN_2; 3; .

Searching in 'World-2DPAGE Repository [0014]' for entry matching: P85972

World-2DPAGE Repository (0014): P85972

P85972

General information about the entry

Entry name

VINC_RAT

Primary accession number

P85972

integrated into World-2DPAGE Repository (0014) on

October 13, 2008 (release 1)

2D Annotations were last modified on

June 1, 2011 (version 3)

General Annotations were last modified on

November 25, 2011 (version 2)

Name and origin of the protein

Description

RecName: Full=Vinculin; AltName: Full=Metavinculin;.

Gene name

Name=Vcl

Annotated species

Rattus norvegicus (Rat) [TaxID: 10116]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	2D-PAGE GEL CHARACTERIZATION DOI=10.1002/pmic.200900680; Maurya D.K., Sundaram C.S., Bhargava P. ''Proteome profile of whole cerebellum of the mature rat'' Proteomics 10(23):4311-4319 (2010)

2D PAGE maps for identified proteins

RAT_CEREBELLUM_21D {Proteome of 21 days old rat cerebellum}
Rattus norvegicus (Rat)
Tissue: Cerebellum

RAT_CEREBELLUM_21D

MAP LOCATIONS:

SPOT 2665:

pI=6.62; Mw=91000

IDENTIFICATION: SPOT 2665: SeqCov=34%. MascotPMF=215 [1].
MAPPING (identification):

SPOT 2665: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Purnima Bhargava, Centre for Cellular and Molecular Biology, India

Cross-references

PRIDE

10017.

UniProtKB/Swiss-Prot

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry name	VINC_RAT
Primary accession number	P85972
Secondary accession number(s)	A6KKR4
Sequence was last modified on	September 2, 2008 (version 1)
Annotations were last modified on	November 16, 2011 (version 26)
Name and origin of the protein
Description	RecName: Full=Vinculin; AltName: Full=Metavinculin;
Gene name	Name=Vcl
Encoded on	Name=Vcl
Keywords	Acetylation; Actin-binding; Cell adhesion; Cell junction; Cell membrane; Complete proteome; Cytoplasm; Cytoskeleton; Lipoprotein; Membrane; Myristate; Palmitate; Phosphoprotein; Reference proteome; Repeat.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	CH474061; EDL86258.1; -; Genomic_DNA
IPI	IPI00365286; -; .
RefSeq	NP_001100718.1; NM_001107248.1; .
UniGene	Rn.164613; -; .
MINT	MINT-6804299; -; .
STRING	P85972; -; .
PhosphoSite	P85972; -; .
World-2DPAGE	0004:P85972; -; .
PRIDE	P85972; -; .
Ensembl	ENSRNOT00000015179; ENSRNOP00000015179; ENSRNOG00000010765; .
GeneID	305679; -; .
KEGG	rno:305679; -; .
CTD	7414; -; .
RGD	1311217; Vcl; .
eggNOG	roNOG04310; -; .
GeneTree	ENSGT00550000074411; -; .
HOVERGEN	HBG079758; -; .
OMA	ELTHQVH; -; .
OrthoDB	EOG4P5K8C; -; .
PhylomeDB	P85972; -; .
Genevestigator	P85972; -; .
GO	GO:0015629; C:actin cytoskeleton; IEA:InterPro; .
GO	GO:0003779; F:actin binding; IEA:UniProtKB-KW; .
GO	GO:0017048; F:Rho GTPase binding; IPI:RGD; .
GO	GO:0005198; F:structural molecule activity; IEA:InterPro; .
InterPro	IPR017997; Vinculin; .
InterPro	IPR006077; Vinculin/catenin; .
InterPro	IPR000633; Vinculin_CS; .
KO	K05700; -; .
Pfam	PF01044; Vinculin; 2; .
PRINTS	PR00806; VINCULIN; .
SUPFAM	SSF47220; Vinculin/catenin; 7; .
PROSITE	PS00663; VINCULIN_1; 1; .
PROSITE	PS00664; VINCULIN_2; 3; .

Searching in 'World-2DPAGE Repository [0022]' for entry matching: P85972

World-2DPAGE Repository (0022): P85972

P85972

General information about the entry

Entry name

VINC_RAT

Primary accession number

P85972

integrated into World-2DPAGE Repository (0022) on

May 7, 2010 (release 1)

2D Annotations were last modified on

June 22, 2011 (version 2)

General Annotations were last modified on

November 24, 2011 (version 2)

Name and origin of the protein

Description

RecName: Full=Vinculin; AltName: Full=Metavinculin;.

Gene name

Name=Vcl

Annotated species

Rattus [TaxID: 10114]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae.

References

[1]

2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000593;
Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L.
''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity''
Proteomics 11(16):3369-3379 (2011)

2D PAGE maps for identified proteins

HCR_LCR_HEART {HCR LCR Cardiac Map}
Rattus
Tissue: Heart

HCR_LCR_HEART

MAP LOCATIONS:

SPOT 482:

pI=5.83; Mw=117112

IDENTIFICATION: SPOT 482: SeqCov=17.7%. Peptides MS=13/20. Mascot PMF Score=92 [1].
PEPTIDE EVIDENCE: SPOT 482: 833-EAFQPQEPDFPPPPPDLEQLR-853 (66) [1].
MAPPING (identification):

SPOT 482: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Jatin Burniston, Liverpool John Moores University, UK

Cross-references

UniProtKB/Swiss-Prot

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry name	VINC_RAT
Primary accession number	P85972
Secondary accession number(s)	A6KKR4
Sequence was last modified on	September 2, 2008 (version 1)
Annotations were last modified on	November 16, 2011 (version 26)
Name and origin of the protein
Description	RecName: Full=Vinculin; AltName: Full=Metavinculin;
Gene name	Name=Vcl
Encoded on	Name=Vcl
Keywords	Acetylation; Actin-binding; Cell adhesion; Cell junction; Cell membrane; Complete proteome; Cytoplasm; Cytoskeleton; Lipoprotein; Membrane; Myristate; Palmitate; Phosphoprotein; Reference proteome; Repeat.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	CH474061; EDL86258.1; -; Genomic_DNA
IPI	IPI00365286; -; .
RefSeq	NP_001100718.1; NM_001107248.1; .
UniGene	Rn.164613; -; .
MINT	MINT-6804299; -; .
STRING	P85972; -; .
PhosphoSite	P85972; -; .
World-2DPAGE	0004:P85972; -; .
PRIDE	P85972; -; .
Ensembl	ENSRNOT00000015179; ENSRNOP00000015179; ENSRNOG00000010765; .
GeneID	305679; -; .
KEGG	rno:305679; -; .
CTD	7414; -; .
RGD	1311217; Vcl; .
eggNOG	roNOG04310; -; .
GeneTree	ENSGT00550000074411; -; .
HOVERGEN	HBG079758; -; .
OMA	ELTHQVH; -; .
OrthoDB	EOG4P5K8C; -; .
PhylomeDB	P85972; -; .
Genevestigator	P85972; -; .
GO	GO:0015629; C:actin cytoskeleton; IEA:InterPro; .
GO	GO:0003779; F:actin binding; IEA:UniProtKB-KW; .
GO	GO:0017048; F:Rho GTPase binding; IPI:RGD; .
GO	GO:0005198; F:structural molecule activity; IEA:InterPro; .
InterPro	IPR017997; Vinculin; .
InterPro	IPR006077; Vinculin/catenin; .
InterPro	IPR000633; Vinculin_CS; .
KO	K05700; -; .
Pfam	PF01044; Vinculin; 2; .
PRINTS	PR00806; VINCULIN; .
SUPFAM	SSF47220; Vinculin/catenin; 7; .
PROSITE	PS00663; VINCULIN_1; 1; .
PROSITE	PS00664; VINCULIN_2; 3; .

Searching in 'World-2DPAGE Repository [0030]' for entry matching: P85972

World-2DPAGE Repository (0030): P85972

P85972

General information about the entry

Entry name

VINC_RAT

Primary accession number

P85972

integrated into World-2DPAGE Repository (0030) on

November 9, 2010 (release 1)

2D Annotations were last modified on

June 21, 2011 (version 2)

General Annotations were last modified on

November 23, 2011 (version 2)

Name and origin of the protein

Description

RecName: Full=Vinculin; AltName: Full=Metavinculin;.

Gene name

Name=Vcl

Annotated species

Rattus norvegicus (Rat) [TaxID: 10116]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	2D-PAGE GEL CHARACTERIZATION DOI=10.1002/pmic.201000006; D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C. ''Two-dimensional gel proteome reference map of INS-1E cells'' Proteomics 11(7):1365-1369 (2011)

2D PAGE maps for identified proteins

RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7}
Rattus norvegicus (Rat)

RAT_INS1E_4-7

MAP LOCATIONS:

SPOT 196:

pI=5.47; Mw=98143

SPOT 202:

pI=5.42; Mw=98007

IDENTIFICATION: SPOT 196: Mascot protein score=71. Number of peptides=3 [1]
SPOT 202: Mascot protein score=124. Number of peptides=5 [1].
MAPPING (identification):

SPOT 196: Tandem mass spectrometry [1];
SPOT 202: Tandem mass spectrometry [1].

Copyright

Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium

Cross-references

UniProtKB/Swiss-Prot

World-2DPAGE Repository