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Searching in 'World-2DPAGE Repository [0022]' for entry matching: P85972




World-2DPAGE Repository (0022):  P85972


P85972


General information about the entry
View entry in simple text format
Entry nameVINC_RAT
Primary accession numberP85972
integrated into World-2DPAGE Repository (0022) on May 7, 2010 (release 1)
2D Annotations were last modified onJune 22, 2011 (version 2)
General Annotations were last modified on November 24, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Vinculin; AltName: Full=Metavinculin;.
Gene nameName=Vcl
Annotated speciesRattus [TaxID: 10114]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000593;
Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L.
''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity''
Proteomics 11(16):3369-3379 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

HCR_LCR_HEART {HCR LCR Cardiac Map}
Rattus
Tissue: Heart
HCR_LCR_HEART
  map experimental info
 
HCR_LCR_HEART

MAP LOCATIONS:
pI=5.83; Mw=117112  [identification data]

IDENTIFICATION: SPOT 482: SeqCov=17.7%. Peptides MS=13/20. Mascot PMF Score=92 [1].
PEPTIDE EVIDENCE: SPOT 482: 833-EAFQPQEPDFPPPPPDLEQLR-853 (66) [1].
MAPPING (identification):
SPOT 482: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright
Data from Dr. Jatin Burniston, Liverpool John Moores University, UK
Cross-references
UniProtKB/Swiss-ProtP85972; VINC_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameVINC_RAT
Primary accession numberP85972
Secondary accession number(s) A6KKR4
Sequence was last modified on September 2, 2008 (version 1)
Annotations were last modified on November 16, 2011 (version 26)
Name and origin of the protein
DescriptionRecName: Full=Vinculin; AltName: Full=Metavinculin;
Gene nameName=Vcl
Encoded onName=Vcl
KeywordsAcetylation; Actin-binding; Cell adhesion; Cell junction; Cell membrane; Complete proteome; Cytoplasm; Cytoskeleton; Lipoprotein; Membrane; Myristate; Palmitate; Phosphoprotein; Reference proteome; Repeat.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLCH474061; EDL86258.1; -; Genomic_DNA
IPIIPI00365286; -; .
RefSeqNP_001100718.1; NM_001107248.1; .
UniGeneRn.164613; -; .
MINTMINT-6804299; -; .
STRINGP85972; -; .
PhosphoSiteP85972; -; .
World-2DPAGE0004:P85972; -; .
PRIDEP85972; -; .
EnsemblENSRNOT00000015179; ENSRNOP00000015179; ENSRNOG00000010765; .
GeneID305679; -; .
KEGGrno:305679; -; .
CTD7414; -; .
RGD1311217; Vcl; .
eggNOGroNOG04310; -; .
GeneTreeENSGT00550000074411; -; .
HOVERGENHBG079758; -; .
OMAELTHQVH; -; .
OrthoDBEOG4P5K8C; -; .
PhylomeDBP85972; -; .
GenevestigatorP85972; -; .
GOGO:0015629; C:actin cytoskeleton; IEA:InterPro; .
GOGO:0003779; F:actin binding; IEA:UniProtKB-KW; .
GOGO:0017048; F:Rho GTPase binding; IPI:RGD; .
GOGO:0005198; F:structural molecule activity; IEA:InterPro; .
InterProIPR017997; Vinculin; .
InterProIPR006077; Vinculin/catenin; .
InterProIPR000633; Vinculin_CS; .
KOK05700; -; .
PfamPF01044; Vinculin; 2; .
PRINTSPR00806; VINCULIN; .
SUPFAMSSF47220; Vinculin/catenin; 7; .
PROSITEPS00663; VINCULIN_1; 1; .
PROSITEPS00664; VINCULIN_2; 3; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server