World-2DPAGE Home
Home  |  Contact
Due to scheduled maintenance work, this service will not be available from Tuesday August 23rd 06:00 pm until Wednesday August 24th 08:00 am CEST. Apologies for the inconvenience.

World-2DPAGE Repository 
Search by  [accession number] *
[description, ID or gene] 
[author names] 
[spot ID / serial number] 
[identification methods] 
[pI / Mw range] 
[combined fields] 
Maps  [experimental info] 
[protein list] 
[graphical interface]
     Databases

use 'Ctrl' to select several
databases

Select Remote Interfaces
[All Interfaces]
SWISS-2DPAGE
World-2DPAGE Portal
Exclude local DBs
has only effect if a remote
interface is selected
     
Searching in 'World-2DPAGE Repository [0030]' for entry matching: PSA1_RAT




World-2DPAGE Repository (0030):  PSA1_RAT

PSA1_RAT


General information about the entry
View entry in simple text format
Entry namePSA1_RAT
Primary accession numberP18420
integrated into World-2DPAGE Repository (0030) on November 9, 2010 (release 1)
2D Annotations were last modified onJune 21, 2011 (version 2)
General Annotations were last modified on November 23, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Proteasome subunit alpha type-1; EC=3.4.25.1; AltName: Full=Macropain subunit C2; AltName: Full=Multicatalytic endopeptidase complex subunit C2; AltName: Full=Proteasome component C2; AltName: Full=Proteasome nu chain;.
Gene nameName=Psma1
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000006;
D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C.
''Two-dimensional gel proteome reference map of INS-1E cells''
Proteomics 11(7):1365-1369 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7}
Rattus norvegicus (Rat)
RAT_INS1E_4-7
  map experimental info 		 
RAT_INS1E_4-7

MAP LOCATIONS:
pI=6.46; Mw=33499  [identification data]
pI=6.45; Mw=32857  [identification data]

IDENTIFICATION: SPOT 1926: Mascot protein score=120. Number of peptides=2 [1]
SPOT 1948: Mascot protein score=150. Number of peptides=3 [1].
MAPPING (identification):
SPOT 1926: Tandem mass spectrometry [1];
SPOT 1948: Tandem mass spectrometry [1].

Copyright
Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium
Cross-references
UniProtKB/Swiss-ProtP18420; PSA1_RAT.
World-2DPAGE RepositoryP18420; PSA1_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry namePSA1_RAT
Primary accession numberP18420
Sequence was last modified on February 1, 1991 (version 2)
Annotations were last modified on November 16, 2011 (version 106)
Name and origin of the protein
DescriptionRecName: Full=Proteasome subunit alpha type-1; EC=3.4.25.1; AltName: Full=Macropain subunit C2; AltName: Full=Multicatalytic endopeptidase complex subunit C2; AltName: Full=Proteasome component C2; AltName: Full=Proteasome nu chain;
Gene nameName=Psma1
Encoded onName=Psma1
KeywordsAcetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Hydrolase; Immunity; Isopeptide bond; Nucleus; Phosphoprotein; Protease; Proteasome; Reference proteome; Threonine protease; Ubl conjugation.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM29859; AAA41943.1; -; mRNA
EMBLD90265; BAA14312.1; -; mRNA
EMBLBC062233; AAH62233.1; -; mRNA
IPIIPI00191748; -; .
PIRA32968; SNRTC2; .
RefSeqNP_058974.1; NM_017278.1; .
UniGeneRn.2668; -; .
ProteinModelPortalP18420; -; .
SMRP18420; 4-241; .
IntActP18420; 1; .
STRINGP18420; -; .
MEROPST01.976; -; .
World-2DPAGE0004:P18420; -; .
PRIDEP18420; -; .
EnsemblENSRNOT00000015946; ENSRNOP00000015946; ENSRNOG00000011745; .
GeneID29668; -; .
KEGGrno:29668; -; .
NMPDRfig|10116.3.peg.2814; -; .
UCSCNM_017278; rat; .
CTD5682; -; .
RGD61841; Psma1; .
eggNOGroNOG10559; -; .
GeneTreeENSGT00550000074855; -; .
HOVERGENHBG105373; -; .
InParanoidP18420; -; .
OrthoDBEOG4S1T7S; -; .
PhylomeDBP18420; -; .
NextBio609983; -; .
ArrayExpressP18420; -; .
GenevestigatorP18420; -; .
GermOnlineENSRNOG00000011745; Rattus norvegicus; .
GOGO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell; .
GOGO:0005634; C:nucleus; IEA:UniProtKB-SubCell; .
GOGO:0019773; C:proteasome core complex; alpha-subunit complex; IEA:InterPro
GOGO:0004298; F:threonine-type endopeptidase activity; IEA:UniProtKB-KW; .
GOGO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro; .
InterProIPR000426; Proteasome_asu_CS; .
InterProIPR023332; Proteasome_suA-type; .
InterProIPR001353; Proteasome_sua/b; .
KOK02725; -; .
PfamPF00227; Proteasome; 1; .
PfamPF10584; Proteasome_A_N; 1; .
SMARTSM00948; Proteasome_A_N; 1; .
PROSITEPS00388; PROTEASOME_A_1; 1; .
PROSITEPS51475; PROTEASOME_A_2; 1; .


World-2DPAGE Repository image

World-2DPAGE Repository (search AC)

Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server