World-2DPAGE Repository

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	MAPPING ON GEL PubMed=19343716; DOI=10.1002/pmic.200800664; [NCBI, EBI, Israel, Japan] Maurya D.K., Sundaram C.S., Bhargava P. ''Proteome profile of the mature rat olfactory bulb'' Proteomics 9(1):2593-2599 (2009)

2D PAGE maps for identified proteins

How to interpret a protein

OB21DSUB {2-DE gel for Olfactory bulb proteome}
Rattus norvegicus (Rat)
Tissue: Olfactory bulb

map experimental info

OB21DSUB

MAP LOCATIONS:

SPOT 71:

pI=6.23; Mw=95000

[identification data]

MAPPING (identification):

SPOT 71: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Purnima Bhargava, Centre for Cellular and Molecular Biology, India

Cross-references

UniProtKB/Swiss-Prot

P52873; PYC_RAT.

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry name	PYC_RAT
Primary accession number	P52873
Secondary accession number(s)	Q5RKM0 Q64555
Sequence was last modified on	May 1, 2007 (version 2)
Annotations were last modified on	November 16, 2011 (version 112)
Name and origin of the protein
Description	RecName: Full=Pyruvate carboxylase, mitochondrial; EC=6.4.1.1; AltName: Full=Pyruvic carboxylase; Short=PCB; Flags: Precursor;
Gene name	Name=Pc
Encoded on	Name=Pc
Keywords	Acetylation; ATP-binding; Biotin; Complete proteome; Direct protein sequencing; Gluconeogenesis; Ligase; Lipid synthesis; Manganese; Metal-binding; Mitochondrion; Multifunctional enzyme; Nucleotide-binding; Phosphoprotein; Pyruvate; Reference proteome; Transit peptide.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	U32314; AAA96256.1; -; mRNA
EMBL	U36585; AAC52668.1; -; mRNA
EMBL	BC085680; AAH85680.1; -; mRNA
IPI	IPI00210435; -; .
PIR	S68252; JC4391; .
RefSeq	NP_036876.2; NM_012744.2; .
UniGene	Rn.11094; -; .
ProteinModelPortal	P52873; -; .
SMR	P52873; 35-490; 494-1178; .
MINT	MINT-4592965; -; .
STRING	P52873; -; .
World-2DPAGE	0004:P52873; -; .
PRIDE	P52873; -; .
Ensembl	ENSRNOT00000026316; ENSRNOP00000026316; ENSRNOG00000019372; .
GeneID	25104; -; .
KEGG	rno:25104; -; .
NMPDR	fig\|10116.3.peg.3454; -; .
UCSC	NM_012744; rat; .
CTD	5091; -; .
RGD	3262; Pc; .
eggNOG	roNOG05375; -; .
GeneTree	ENSGT00550000074986; -; .
HOVERGEN	HBG008340; -; .
InParanoid	P52873; -; .
OMA	YKADEAY; -; .
OrthoDB	EOG4ZCT3P; -; .
PhylomeDB	P52873; -; .
NextBio	605431; -; .
ArrayExpress	P52873; -; .
Genevestigator	P52873; -; .
GermOnline	ENSRNOG00000019372; Rattus norvegicus; .
GO	GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell; .
GO	GO:0005625; C:soluble fraction; IDA:RGD; .
GO	GO:0005524; F:ATP binding; IDA:RGD; .
GO	GO:0009374; F:biotin binding; IMP:RGD; .
GO	GO:0004075; F:biotin carboxylase activity; IEA:InterPro; .
GO	GO:0046872; F:metal ion binding; IEA:UniProtKB-KW; .
GO	GO:0004736; F:pyruvate carboxylase activity; IDA:RGD; .
GO	GO:0006094; P:gluconeogenesis; IDA:RGD; .
GO	GO:0008610; P:lipid biosynthetic process; IEA:UniProtKB-KW; .
GO	GO:0006107; P:oxaloacetate metabolic process; IDA:RGD; .
GO	GO:0006090; P:pyruvate metabolic process; IDA:RGD; .
InterPro	IPR013785; Aldolase_TIM; .
InterPro	IPR011761; ATP-grasp; .
InterPro	IPR013815; ATP_grasp_subdomain_1; .
InterPro	IPR013816; ATP_grasp_subdomain_2; .
InterPro	IPR001882; Biotin_BS; .
InterPro	IPR011764; Biotin_carboxylation_dom; .
InterPro	IPR005482; Biotin_COase_C; .
InterPro	IPR000089; Biotin_lipoyl; .
InterPro	IPR005479; CarbamoylP_synth_lsu_ATP-bd; .
InterPro	IPR005481; CarbamoylP_synth_lsu_N; .
InterPro	IPR003379; Carboxylase_cons_dom; .
InterPro	IPR013817; Pre-ATP_grasp; .
InterPro	IPR016185; PreATP-grasp-like; .
InterPro	IPR000891; PYR_CT; .
InterPro	IPR005930; Pyruv_COase; .
InterPro	IPR011054; Rudment_hybrid_motif; .
InterPro	IPR011053; Single_hybrid_motif; .
Gene3D	G3DSA:3.20.20.70; Aldolase_TIM; 1; .
Gene3D	G3DSA:3.30.1490.20; ATP_grasp_subdomain_1; 1; .
Gene3D	G3DSA:3.30.470.20; ATP_grasp_subdomain_2; 1; .
Gene3D	G3DSA:3.40.50.20; Pre-ATP_grasp; 1; .
KO	K01958; -; .
PANTHER	PTHR18866:SF10; PTHR18866:SF10; 1; .
Pfam	PF02785; Biotin_carb_C; 1; .
Pfam	PF00364; Biotin_lipoyl; 1; .
Pfam	PF00289; CPSase_L_chain; 1; .
Pfam	PF02786; CPSase_L_D2; 1; .
Pfam	PF00682; HMGL-like; 1; .
Pfam	PF02436; PYC_OADA; 1; .
PIRSF	PIRSF001594; Pyruv_carbox; 1; .
SMART	SM00878; Biotin_carb_C; 1; .
SUPFAM	SSF51230; Hybrid_motif; 1; .
SUPFAM	SSF52440; PreATP-grasp-like; 1; .
SUPFAM	SSF51246; Rudmnt_hyb_motif; 1; .
TIGRFAMs	TIGR01235; Pyruv_carbox; 1; .
PROSITE	PS50975; ATP_GRASP; 1; .
PROSITE	PS50979; BC; 1; .
PROSITE	PS00188; BIOTIN; 1; .
PROSITE	PS50968; BIOTINYL_LIPOYL; 1; .
PROSITE	PS50991; PYR_CT; 1; .

World-2DPAGE Repository (search AC)

Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server

0001
0002
0003
0004
0005
0006
0007
0008
0009
0010
0011
0012
0014
0017
0018
0019
0020
0021
0022
0023
0024
0026
0027
0029
0030
0031
0032
0034
0035
0036
0037
0038
0039
0040
0042
0043
0044
0046
0047
0050
0051
0053
0054
0056
0059
0061
0062
0063
0066
0068
0069
0076
0077
0080
0084
0085
0088