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Searching in 'World-2DPAGE Repository [0022]' for entry matching: PYGB_RAT




World-2DPAGE Repository (0022):  PYGB_RAT


PYGB_RAT


General information about the entry
View entry in simple text format
Entry namePYGB_RAT
Primary accession numberP53534
integrated into World-2DPAGE Repository (0022) on May 7, 2010 (release 1)
2D Annotations were last modified onJune 22, 2011 (version 2)
General Annotations were last modified on November 24, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Glycogen phosphorylase, brain form; EC=2.4.1.1; Flags: Fragment;.
Gene nameName=Pygb
Annotated speciesRattus [TaxID: 10114]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000593;
Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L.
''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity''
Proteomics 11(16):3369-3379 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

HCR_LCR_HEART {HCR LCR Cardiac Map}
Rattus
Tissue: Heart
HCR_LCR_HEART
  map experimental info
 
HCR_LCR_HEART

MAP LOCATIONS:
pI=6.24; Mw=96854  [identification data]
pI=6.24; Mw=96854  [identification data]
pI=6.24; Mw=96854  [identification data]
pI=6.24; Mw=96854  [identification data]

IDENTIFICATION: SPOT 523: SeqCov=19.6%. Peptides MS=14/20. Mascot PMF Score=105 [1]
SPOT 728: SeqCov=20.4%. Peptides MS=14/20. Mascot PMF Score=106 [1]; SPOT 787: SeqCov=20.4%. Peptides MS=15/20. Mascot PMF Score=113 [1]; SPOT 939: SeqCov=19.3%. Peptides MS=14/20. Mascot PMF Score=103 [1].
PEPTIDE EVIDENCE: SPOT 523: 255-LKDFNVGDYIEAVLDR-270 (30) / 294-LKQEYFVVAATLQDIIR-310 (29) [1]
SPOT 728: 51-DYFFALAHTVR-61 (54) / 400-HLEIIYAINQR-410 (55) / 294-LKQEYFVVAATLQDIIR-310 (53) [1]; SPOT 787: 294-LKQEYFVVAATLQDIIR-310 (55) [1]; SPOT 939: 236-NNTVNTMR-243 (1) / 400-HLEIIYAINQR-410 (42) / 774-VFADYEAYIQCQAQVDHLYR-793 (6) [1].
MAPPING (identification):
SPOT 523: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 728: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 787: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 939: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright
Data from Dr. Jatin Burniston, Liverpool John Moores University, UK
Cross-references
UniProtKB/Swiss-ProtP53534; PYGB_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry namePYGB_RAT
Primary accession numberP53534
Sequence was last modified on January 23, 2007 (version 3)
Annotations were last modified on November 16, 2011 (version 90)
Name and origin of the protein
DescriptionRecName: Full=Glycogen phosphorylase, brain form; EC=2.4.1.1; Flags: Fragment;
Gene nameName=Pygb
Encoded onName=Pygb
KeywordsAcetylation; Allosteric enzyme; Carbohydrate metabolism; Complete proteome; Direct protein sequencing; Glycogen metabolism; Glycosyltransferase; Phosphoprotein; Pyridoxal phosphate; Reference proteome; Transferase.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLL10668; AAA41252.1; -; mRNA
EMBLM27726; AAA40815.1; -; mRNA
IPIIPI00948869; -; .
PIRS37300; S37300; .
RefSeqNP_037320.1; NM_013188.1; .
UniGeneRn.1518; -; .
ProteinModelPortalP53534; -; .
SMRP53534; 14-838; .
IntActP53534; 1; .
STRINGP53534; -; .
CAZyGT35; Glycosyltransferase Family 35; .
PhosphoSiteP53534; -; .
World-2DPAGE0004:P53534; -; .
PRIDEP53534; -; .
GeneID25739; -; .
KEGGrno:25739; -; .
CTD5834; -; .
RGD3460; Pygb; .
eggNOGroNOG12045; -; .
GeneTreeENSGT00390000016886; -; .
HOVERGENHBG006848; -; .
InParanoidP53534; -; .
PhylomeDBP53534; -; .
NextBio607887; -; .
ArrayExpressP53534; -; .
GenevestigatorP53534; -; .
GermOnlineENSRNOG00000007583; Rattus norvegicus; .
GOGO:0030424; C:axon; IDA:RGD; .
GOGO:0005625; C:soluble fraction; IDA:RGD; .
GOGO:0008144; F:drug binding; IDA:RGD; .
GOGO:0008184; F:glycogen phosphorylase activity; IDA:RGD; .
GOGO:0042803; F:protein homodimerization activity; IDA:RGD; .
GOGO:0030170; F:pyridoxal phosphate binding; IEA:InterPro; .
GOGO:0005529; F:sugar binding; IDA:RGD; .
GOGO:0005980; P:glycogen catabolic process; IDA:RGD; .
InterProIPR011833; Glycg_phsphrylas; .
InterProIPR000811; Glyco_trans_35; .
KOK00688; -; .
PANTHERPTHR11468; Glyco_trans_35; 1; .
PfamPF00343; Phosphorylase; 1; .
PIRSFPIRSF000460; Pprylas_GlgP; 1; .
TIGRFAMsTIGR02093; P_ylase; 1; .
PROSITEPS00102; PHOSPHORYLASE; 1; .



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