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World-2DPAGE Repository

Attention: World-2DPAGE is no longer maintained. Previously submitted data can still be queried at World-2DPAGE Repository.

World-2DPAGE Repository 
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Searching in 'World-2DPAGE Repository [0022]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0022):  PYGM_RAT

PYGM_RAT


General information about the entry
View entry in simple text format
Entry namePYGM_RAT
Primary accession numberP09812
integrated into World-2DPAGE Repository (0022) on May 7, 2010 (release 1)
2D Annotations were last modified onJune 22, 2011 (version 2)
General Annotations were last modified on November 24, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Glycogen phosphorylase, muscle form; EC=2.4.1.1; AltName: Full=Myophosphorylase;.
Gene nameName=Pygm
Annotated speciesRattus [TaxID: 10114]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000593;
Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L.
''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity''
Proteomics 11(16):3369-3379 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

HCR_LCR_HEART {HCR LCR Cardiac Map}
Rattus
Tissue: Heart
HCR_LCR_HEART
  map experimental info 		 
HCR_LCR_HEART

MAP LOCATIONS:
pI=6.91; Mw=97725  [identification data]
pI=6.91; Mw=97725  [identification data]
pI=6.91; Mw=97725  [identification data]
pI=6.91; Mw=97725  [identification data]
pI=6.91; Mw=97725  [identification data]
pI=6.91; Mw=97725  [identification data]
pI=6.91; Mw=97725  [identification data]

IDENTIFICATION: SPOT 47: SeqCov=22.6%. Peptides MS=15/20. Mascot PMF Score=123 [1]
SPOT 79: SeqCov=24.8%. Peptides MS=17/20. Mascot PMF Score=145 [1]; SPOT 134: SeqCov=22.9%. Peptides MS=16/20. Mascot PMF Score=140 [1]; SPOT 224: SeqCov=15.2%. Peptides MS=9/20. Mascot PMF Score=53 [1]; SPOT 270: SeqCov=20.2%. Peptides MS=14/20. Mascot PMF Score=111 [1]; SPOT 464: SeqCov=15%. Peptides MS=10/20. Mascot PMF Score=62 [1]; SPOT 605: SeqCov=19.4%. Peptides MS=13/20. Mascot PMF Score=99 [1].
PEPTIDE EVIDENCE: SPOT 224: 193-ARPEFTLPVHFYGR-206 (68) / 294-LKQEYFVVAATLQDIIR-310 (28) [1]
SPOT 270: 257-DFNVGGYIQAVLDR-270 (104) / 193-ARPEFTLPVHFYGR-206 (62) / 294-LKQEYFVVAATLQDIIR-310 (33) [1]; SPOT 464: 257-DFNVGGYIQAVLDR-270 (61) / 193-ARPEFTLPVHFYGR-206 (71) / 294-LKQEYFVVAATLQDIIR-310 (60) [1]; SPOT 605: 193-ARPEFTLPVHFYGR-206 (71) / 294-LKQEYFVVAATLQDIIR-310 (39) [1].
MAPPING (identification):
SPOT 47: Peptide mass fingerprinting [1];
SPOT 79: Peptide mass fingerprinting [1];
SPOT 134: Peptide mass fingerprinting [1];
SPOT 224: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 270: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 464: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 605: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright
Data from Dr. Jatin Burniston, Liverpool John Moores University, UK
Cross-references
UniProtKB/Swiss-ProtP09812; PYGM_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry namePYGM_RAT
Primary accession numberP09812
Sequence was last modified on January 23, 2007 (version 5)
Annotations were last modified on October 19, 2011 (version 102)
Name and origin of the protein
DescriptionRecName: Full=Glycogen phosphorylase, muscle form; EC=2.4.1.1; AltName: Full=Myophosphorylase;
Gene nameName=Pygm
Encoded onName=Pygm
KeywordsAcetylation; Allosteric enzyme; Carbohydrate metabolism; Complete proteome; Glycogen metabolism; Glycosyltransferase; Nucleotide-binding; Phosphoprotein; Pyridoxal phosphate; Reference proteome; Transferase.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLL10669; AAA41253.1; -; mRNA
EMBLX03032; CAA26835.1; -; mRNA
IPIIPI00190181; -; .
PIRS34624; S34624; .
UniGeneRn.11238; -; .
ProteinModelPortalP09812; -; .
SMRP09812; 14-842; .
STRINGP09812; -; .
CAZyGT35; Glycosyltransferase Family 35; .
PhosphoSiteP09812; -; .
PRIDEP09812; -; .
RGD3461; Pygm; .
eggNOGroNOG12045; -; .
HOVERGENHBG006848; -; .
InParanoidP09812; -; .
OrthoDBEOG4S1T6F; -; .
PhylomeDBP09812; -; .
ArrayExpressP09812; -; .
GenevestigatorP09812; -; .
GermOnlineENSRNOG00000021090; Rattus norvegicus; .
GOGO:0016529; C:sarcoplasmic reticulum; IDA:RGD; .
GOGO:0005625; C:soluble fraction; IDA:RGD; .
GOGO:0016208; F:AMP binding; IDA:RGD; .
GOGO:0008144; F:drug binding; IDA:RGD; .
GOGO:0008184; F:glycogen phosphorylase activity; IDA:RGD; .
GOGO:0042803; F:protein homodimerization activity; IC:RGD; .
GOGO:0030170; F:pyridoxal phosphate binding; IDA:RGD; .
GOGO:0005529; F:sugar binding; IDA:RGD; .
GOGO:0006874; P:cellular calcium ion homeostasis; IDA:RGD; .
GOGO:0005980; P:glycogen catabolic process; IDA:RGD; .
GOGO:0051591; P:response to cAMP; IDA:RGD; .
GOGO:0001666; P:response to hypoxia; IDA:RGD; .
InterProIPR011833; Glycg_phsphrylas; .
InterProIPR000811; Glyco_trans_35; .
PANTHERPTHR11468; Glyco_trans_35; 1; .
PfamPF00343; Phosphorylase; 1; .
PIRSFPIRSF000460; Pprylas_GlgP; 1; .
TIGRFAMsTIGR02093; P_ylase; 1; .
PROSITEPS00102; PHOSPHORYLASE; 1; .





Searching in 'World-2DPAGE Repository [0001]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0001):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0001)



Searching in 'World-2DPAGE Repository [0002]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0002):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0002)



Searching in 'World-2DPAGE Repository [0003]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0003):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0003)



Searching in 'World-2DPAGE Repository [0004]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0004):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0004)



Searching in 'World-2DPAGE Repository [0005]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0005):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0005)



Searching in 'World-2DPAGE Repository [0006]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0006):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0006)



Searching in 'World-2DPAGE Repository [0007]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0007):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0007)



Searching in 'World-2DPAGE Repository [0008]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0008):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0008)



Searching in 'World-2DPAGE Repository [0009]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0009):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0009)



Searching in 'World-2DPAGE Repository [0010]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0010):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0010)



Searching in 'World-2DPAGE Repository [0011]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0011):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0011)



Searching in 'World-2DPAGE Repository [0012]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0012):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0012)



Searching in 'World-2DPAGE Repository [0014]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0014):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0014)



Searching in 'World-2DPAGE Repository [0017]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0017):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0017)



Searching in 'World-2DPAGE Repository [0018]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0018):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0018)



Searching in 'World-2DPAGE Repository [0019]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0019):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0019)



Searching in 'World-2DPAGE Repository [0020]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0020):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0020)



Searching in 'World-2DPAGE Repository [0021]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0021):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0021)



Searching in 'World-2DPAGE Repository [0023]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0023):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0023)



Searching in 'World-2DPAGE Repository [0024]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0024):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0024)



Searching in 'World-2DPAGE Repository [0026]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0026):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0026)



Searching in 'World-2DPAGE Repository [0027]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0027):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0027)



Searching in 'World-2DPAGE Repository [0029]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0029):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0029)



Searching in 'World-2DPAGE Repository [0030]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0030):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0030)



Searching in 'World-2DPAGE Repository [0031]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0031):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0031)



Searching in 'World-2DPAGE Repository [0032]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0032):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0032)



Searching in 'World-2DPAGE Repository [0034]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0034):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0034)



Searching in 'World-2DPAGE Repository [0035]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0035):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0035)



Searching in 'World-2DPAGE Repository [0036]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0036):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0036)



Searching in 'World-2DPAGE Repository [0037]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0037):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0037)



Searching in 'World-2DPAGE Repository [0038]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0038):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0038)



Searching in 'World-2DPAGE Repository [0039]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0039):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0039)



Searching in 'World-2DPAGE Repository [0040]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0040):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0040)



Searching in 'World-2DPAGE Repository [0042]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0042):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0042)



Searching in 'World-2DPAGE Repository [0043]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0043):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0043)



Searching in 'World-2DPAGE Repository [0044]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0044):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0044)



Searching in 'World-2DPAGE Repository [0046]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0046):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0046)



Searching in 'World-2DPAGE Repository [0047]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0047):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0047)



Searching in 'World-2DPAGE Repository [0050]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0050):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0050)



Searching in 'World-2DPAGE Repository [0051]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0051):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0051)



Searching in 'World-2DPAGE Repository [0053]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0053):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0053)



Searching in 'World-2DPAGE Repository [0054]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0054):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0054)



Searching in 'World-2DPAGE Repository [0056]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0056):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0056)



Searching in 'World-2DPAGE Repository [0059]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0059):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0059)



Searching in 'World-2DPAGE Repository [0061]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0061):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0061)



Searching in 'World-2DPAGE Repository [0062]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0062):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0062)



Searching in 'World-2DPAGE Repository [0063]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0063):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0063)



Searching in 'World-2DPAGE Repository [0066]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0066):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0066)



Searching in 'World-2DPAGE Repository [0068]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0068):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0068)



Searching in 'World-2DPAGE Repository [0069]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0069):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0069)



Searching in 'World-2DPAGE Repository [0076]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0076):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0076)



Searching in 'World-2DPAGE Repository [0077]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0077):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0077)



Searching in 'World-2DPAGE Repository [0080]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0080):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0080)



Searching in 'World-2DPAGE Repository [0084]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0084):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0084)



Searching in 'World-2DPAGE Repository [0085]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0085):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0085)



Searching in 'World-2DPAGE Repository [0088]' for entry matching: PYGM_RAT




World-2DPAGE Repository (0088):  PYGM_RAT

This protein does not exist in the current release of World-2DPAGE Repository (0088)



World-2DPAGE Repository image

World-2DPAGE Repository (search AC)

Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server