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Searching in 'World-2DPAGE Repository [0019]' for entry matching: Q01986

World-2DPAGE Repository (0019):  Q01986


General information about the entry
View entry in simple text format
Entry nameMP2K1_RAT
Primary accession numberQ01986
integrated into World-2DPAGE Repository (0019) on December 9, 2009 (release 1)
2D Annotations were last modified onMay 30, 2011 (version 2)
General Annotations were last modified on January 13, 2012 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Dual specificity mitogen-activated protein kinase kinase 1; Short=MAP kinase kinase 1; Short=MAPKK 1; EC=; AltName: Full=ERK activator kinase 1; AltName: Full=MAPK/ERK kinase 1; Short=MEK 1;.
Gene nameName=Map2k1
Synonyms=Mek1, Prkmk1
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
VanGuilder H.D., Yan H., Farley J.A., Sonntag W.E., Freeman W.M.
''Aging alters the expression of neurotransmission-regulating proteins in the hippocampal synaptoproteome''
J of Neurochemistry 113(6):1577-1588 (2010)
2D PAGE maps for identified proteins
How to interpret a protein

HIPP_SYNAP {Rat hippocampal synaptoproteome}
Rattus norvegicus (Rat)
Tissue: Hippocampus
  map experimental info

pI=6.18; Mw=43779  [identification data]
pI=6.18; Mw=43779  [identification data]
pI=6.18; Mw=43779  [identification data]
pI=6.18; Mw=43779  [identification data]

IDENTIFICATION: SPOT 540: Peptides number=4. SeqCov=9.4%. MOWSE score=1.95E+2 [1]
SPOT 541: Peptides number=2. SeqCov=6.4%. MOWSE score=1.95E+2 [1]; SPOT 543: Peptides number=3. SeqCov=9.4%. MOWSE score=7.89E+1 [1]; SPOT 1177: Peptides number=4. SeqCov=6.4%. MOWSE score=4.62E+2 [1].
MAPPING (identification):
SPOT 540: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 541: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 543: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 1177: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Data from Dr. Willard Freeman, Penn State College of Medicine, USA
UniProtKB/Swiss-ProtQ01986; MP2K1_RAT.

2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry nameMP2K1_RAT
Primary accession numberQ01986
Secondary accession number(s) Q5EBD5
Sequence was last modified on January 23, 2007 (version 2)
Annotations were last modified on December 14, 2011 (version 109)
Name and origin of the protein
DescriptionRecName: Full=Dual specificity mitogen-activated protein kinase kinase 1; Short=MAP kinase kinase 1; Short=MAPKK 1; EC=; AltName: Full=ERK activator kinase 1; AltName: Full=MAPK/ERK kinase 1; Short=MEK 1;
Gene nameName=Map2k1
Synonyms=Mek1, Prkmk1
Encoded onName=Map2k1; Synonyms=Mek1, Prkmk1
KeywordsATP-binding; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Kinase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase; Tyrosine-protein kinase.
Copyrighted by the UniProt Consortium, see Distributed under the Creative Commons Attribution-NoDerivs License
EMBLZ16415; CAA78905.1; -; mRNA
EMBLD13341; BAA02603.1; -; mRNA
EMBLD14591; BAA03441.1; -; mRNA
EMBLX62313; CAA44192.1; -; mRNA
EMBLBC089772; AAH89772.1; -; mRNA
IPIIPI00231247; -; .
PIRJN0840; JN0840; .
RefSeqNP_113831.1; NM_031643.4; .
UniGeneRn.5850; -; .
ProteinModelPortalQ01986; -; .
SMRQ01986; 62-382; .
STRINGQ01986; -; .
PhosphoSiteQ01986; -; .
EnsemblENSRNOT00000013933; ENSRNOP00000013933; ENSRNOG00000010176; .
GeneID170851; -; .
KEGGrno:170851; -; .
UCSCNM_031643; rat; .
CTD5604; -; .
RGD70495; Map2k1; .
eggNOGroNOG05418; -; .
GeneTreeENSGT00570000078767; -; .
HOVERGENHBG108518; -; .
InParanoidQ01986; -; .
OrthoDBEOG4SF965; -; .
PhylomeDBQ01986; -; .
BRENDA2.7.12.2; 5301; .
ReactomeREACT_109781; Immune System; .
ReactomeREACT_111984; Signal Transduction; .
ReactomeREACT_112697; Developmental Biology; .
NextBio621291; -; .
ArrayExpressQ01986; -; .
GenevestigatorQ01986; -; .
GermOnlineENSRNOG00000010176; Rattus norvegicus; .
GOGO:0033267; C:axon part; IDA:RGD; .
GOGO:0005938; C:cell cortex; IDA:RGD; .
GOGO:0005829; C:cytosol; IDA:RGD; .
GOGO:0032839; C:dendrite cytoplasm; IDA:RGD; .
GOGO:0005794; C:Golgi apparatus; IDA:RGD; .
GOGO:0043204; C:perikaryon; IDA:RGD; .
GOGO:0048471; C:perinuclear region of cytoplasm; IDA:RGD; .
GOGO:0005886; C:plasma membrane; IDA:RGD; .
GOGO:0005625; C:soluble fraction; IDA:RGD; .
GOGO:0005524; F:ATP binding; IDA:RGD; .
GOGO:0004708; F:MAP kinase kinase activity; IDA:RGD; .
GOGO:0031435; F:mitogen-activated protein kinase kinase kinase binding; IDA:RGD; .
GOGO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW; .
GOGO:0004713; F:protein tyrosine kinase activity; IEA:UniProtKB-KW; .
GOGO:0017016; F:Ras GTPase binding; IPI:RGD; .
GOGO:0000187; P:activation of MAPK activity; IDA:RGD; .
GOGO:0008283; P:cell proliferation; IDA:RGD; .
GOGO:0048313; P:Golgi inheritance; IMP:RGD; .
GOGO:0032402; P:melanosome transport; IDA:RGD; .
GOGO:0007067; P:mitosis; IDA:RGD; .
GOGO:0034111; P:negative regulation of homotypic cell-cell adhesion; IMP:RGD; .
GOGO:0048812; P:neuron projection morphogenesis; IMP:RGD; .
GOGO:0030335; P:positive regulation of cell migration; IMP:RGD; .
GOGO:0032320; P:positive regulation of Ras GTPase activity; IMP:RGD; .
GOGO:0046579; P:positive regulation of Ras protein signal transduction; IMP:MGI; .
GOGO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IMP:RGD; .
GOGO:0003056; P:regulation of vascular smooth muscle contraction; IMP:RGD; .
GOGO:0048678; P:response to axon injury; IMP:RGD; .
GOGO:0051384; P:response to glucocorticoid stimulus; IDA:RGD; .
GOGO:0006979; P:response to oxidative stress; IDA:RGD; .
GOGO:0047496; P:vesicle transport along microtubule; IDA:RGD; .
InterProIPR011009; Kinase-like_dom; .
InterProIPR000719; Prot_kinase_cat_dom; .
InterProIPR017441; Protein_kinase_ATP_BS; .
InterProIPR017442; Se/Thr_kinase-like_dom; .
InterProIPR008271; Ser/Thr_kinase_AS; .
InterProIPR002290; Ser/Thr_kinase_dom; .
KOK04368; -; .
PfamPF00069; Pkinase; 1; .
SMARTSM00220; S_TKc; 1; .
SUPFAMSSF56112; Kinase_like; 1; .

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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server