World-2DPAGE Repository

RecName: Full=Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial; Short=MCCase subunit alpha; EC=6.4.1.4; AltName: Full=3-methylcrotonyl-CoA carboxylase 1; AltName: Full=3-methylcrotonyl-CoA carboxylase biotin-containing subunit; AltName: Full=3-methylcrotonyl-CoA:carbon dioxide ligase subunit alpha; Flags: Precursor;.

Gene name

Name=Mccc1
Synonyms=Mcca

Annotated species

Rattus norvegicus (Rat) [TaxID: 10116]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	2D-PAGE GEL CHARACTERIZATION DOI=10.1002/pmic.200900680; Maurya D.K., Sundaram C.S., Bhargava P. ''Proteome profile of whole cerebellum of the mature rat'' Proteomics 10(23):4311-4319 (2010)

2D PAGE maps for identified proteins

How to interpret a protein

RAT_CEREBELLUM_21D {Proteome of 21 days old rat cerebellum}
Rattus norvegicus (Rat)
Tissue: Cerebellum

map experimental info

RAT_CEREBELLUM_21D

MAP LOCATIONS:

SPOT 3357:

pI=6.35; Mw=81000

[identification data]

IDENTIFICATION: SPOT 3357: SeqCov=35%. MascotPMF=119 [1].
MAPPING (identification):

SPOT 3357: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Purnima Bhargava, Centre for Cellular and Molecular Biology, India

Cross-references

PRIDE

10017.

UniProtKB/Swiss-Prot

Q5I0C3; MCCA_RAT.

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry name	MCCA_RAT
Primary accession number	Q5I0C3
Sequence was last modified on	February 15, 2005 (version 1)
Annotations were last modified on	November 16, 2011 (version 61)
Name and origin of the protein
Description	RecName: Full=Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial; Short=MCCase subunit alpha; EC=6.4.1.4; AltName: Full=3-methylcrotonyl-CoA carboxylase 1; AltName: Full=3-methylcrotonyl-CoA carboxylase biotin-containing subunit; AltName: Full=3-methylcrotonyl-CoA:carbon dioxide ligase subunit alpha; Flags: Precursor;
Gene name	Name=Mccc1 Synonyms=Mcca
Encoded on	Name=Mccc1; Synonyms=Mcca
Keywords	ATP-binding; Biotin; Complete proteome; Ligase; Mitochondrion; Nucleotide-binding; Reference proteome; Transit peptide.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	BC088473; AAH88473.1; -; mRNA
IPI	IPI00372191; -; .
RefSeq	NP_001009653.1; NM_001009653.1; .
UniGene	Rn.15587; -; .
HSSP	P24182; 1BNC; .
ProteinModelPortal	Q5I0C3; -; .
SMR	Q5I0C3; 641-714; .
STRING	Q5I0C3; -; .
PhosphoSite	Q5I0C3; -; .
PRIDE	Q5I0C3; -; .
GeneID	294972; -; .
KEGG	rno:294972; -; .
UCSC	NM_001009653; rat; .
CTD	56922; -; .
RGD	1310615; Mccc1; .
eggNOG	roNOG07266; -; .
GeneTree	ENSGT00550000074675; -; .
HOVERGEN	HBG000555; -; .
InParanoid	Q5I0C3; -; .
OrthoDB	EOG4WQ123; -; .
NextBio	638797; -; .
ArrayExpress	Q5I0C3; -; .
Genevestigator	Q5I0C3; -; .
GO	GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell; .
GO	GO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GO	GO:0004075; F:biotin carboxylase activity; IEA:InterPro; .
GO	GO:0046872; F:metal ion binding; IEA:InterPro; .
GO	GO:0004485; F:methylcrotonoyl-CoA carboxylase activity; IEA:EC; .
InterPro	IPR011761; ATP-grasp; .
InterPro	IPR013815; ATP_grasp_subdomain_1; .
InterPro	IPR013816; ATP_grasp_subdomain_2; .
InterPro	IPR001882; Biotin_BS; .
InterPro	IPR011764; Biotin_carboxylation_dom; .
InterPro	IPR005482; Biotin_COase_C; .
InterPro	IPR000089; Biotin_lipoyl; .
InterPro	IPR005479; CarbamoylP_synth_lsu_ATP-bd; .
InterPro	IPR005481; CarbamoylP_synth_lsu_N; .
InterPro	IPR013817; Pre-ATP_grasp; .
InterPro	IPR016185; PreATP-grasp-like; .
InterPro	IPR011054; Rudment_hybrid_motif; .
InterPro	IPR011053; Single_hybrid_motif; .
Gene3D	G3DSA:3.30.1490.20; ATP_grasp_subdomain_1; 1; .
Gene3D	G3DSA:3.30.470.20; ATP_grasp_subdomain_2; 1; .
Gene3D	G3DSA:3.40.50.20; Pre-ATP_grasp; 1; .
KO	K01968; -; .
Pfam	PF02785; Biotin_carb_C; 1; .
Pfam	PF00364; Biotin_lipoyl; 1; .
Pfam	PF00289; CPSase_L_chain; 1; .
Pfam	PF02786; CPSase_L_D2; 1; .
SMART	SM00878; Biotin_carb_C; 1; .
SUPFAM	SSF51230; Hybrid_motif; 1; .
SUPFAM	SSF52440; PreATP-grasp-like; 1; .
SUPFAM	SSF51246; Rudmnt_hyb_motif; 1; .
PROSITE	PS50975; ATP_GRASP; 1; .
PROSITE	PS50979; BC; 1; .
PROSITE	PS00188; BIOTIN; 1; .
PROSITE	PS50968; BIOTINYL_LIPOYL; 1; .
PROSITE	PS00866; CPSASE_1; FALSE_NEG; .
PROSITE	PS00867; CPSASE_2; 1; .

World-2DPAGE Repository (search AC)

Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server

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