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World-2DPAGE Repository  Search by  [accession number]  [description, ID or gene]  [author names]  [spot ID / serial number]  [identification methods]  [pI / Mw range]  [combined fields]  Maps  [experimental info]  [protein list]  [graphical interface]      Databases 0001 0002 0003 0004 0005 0006 0007 0008 0009 0010 0011 0012 0014 0017 0018 0019 0020 0021 0022 0023 0024 0026 0027 0029 0030 0031 0032 0034 0035 0036 0037 0038 0039 0040 0042 0043 0044 0046 0047 0050 0051 0053 0054 0056 0059 0061 0062 0063 0066 0068 0069 0076 0077 0080 0084 0085 0088 use 'Ctrl' to select several databases Select Remote Interfaces [All Interfaces] SWISS-2DPAGE World-2DPAGE Portal Exclude local DBs has only effect if a remote interface is selected

Searching in 'World-2DPAGE Repository [0022]' for entry matching: Q5I0C3 World-2DPAGE Repository (0022):  Q5I0C3 Q5I0C3 General information about the entry View entry in simple text format Entry name MCCA_RAT Primary accession number Q5I0C3 integrated into World-2DPAGE Repository (0022) on  May 7, 2010 (release 1) 2D Annotations were last modified on June 22, 2011 (version 2) General Annotations were last modified on  November 24, 2011 (version 2) Name and origin of the protein Description RecName: Full=Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial; Short=MCCase subunit alpha; EC=6.4.1.4; AltName: Full=3-methylcrotonyl-CoA carboxylase 1; AltName: Full=3-methylcrotonyl-CoA carboxylase biotin-containing subunit; AltName: Full=3-methylcrotonyl-CoA:carbon dioxide ligase subunit alpha; Flags: Precursor;. Gene name Name=Mccc1 Synonyms=Mcca Annotated species Rattus [TaxID: 10114] Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae. References [1]   2D-PAGE GEL CHARACTERIZATION DOI=10.1002/pmic.201000593; Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L. ''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity'' Proteomics 11(16):3369-3379 (2011) 2D PAGE maps for identified proteins How to interpret a protein HCR_LCR_HEART {HCR LCR Cardiac Map} Rattus Tissue: Heart   map experimental info   HCR_LCR_HEART MAP LOCATIONS: SPOT 53: pI=6.66; Mw=79564   [identification data] SPOT 854: pI=6.66; Mw=79564   [identification data] SPOT 882: pI=6.66; Mw=79564   [identification data] IDENTIFICATION: SPOT 53: SeqCov=21.7%. Peptides MS=10/20. Mascot PMF Score=72 [1] SPOT 854: SeqCov=14.4%. Peptides MS=15/20. Mascot PMF Score=37 [1]; SPOT 882: SeqCov=26.3%. Peptides MS=16/20. Mascot PMF Score=133 [1]. PEPTIDE EVIDENCE: SPOT 53: 524-LHTQDQFSPFSFSSGR-539 (107) / 364-IPLSQEEIPLQGHAFEAR-381 (105) / 251-HVEVQVFGDHHGNAVYLFER-270 (87) [1] SPOT 854: 251-HVEVQVFGDHHGNAVYLFER-270 (56) [1]. MAPPING (identification): SPOT 53: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1]; SPOT 854: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1]; SPOT 882: Peptide mass fingerprinting [1]. Copyright Data from Dr. Jatin Burniston, Liverpool John Moores University, UK Cross-references UniProtKB/Swiss-Prot Q5I0C3; MCCA_RAT. 2D PAGE maps for identified proteins How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed. External data extracted from UniProtKB/Swiss-Prot Extracted from UniProtKB/Swiss-Prot, release: 2011_11 Entry name MCCA_RAT Primary accession number Q5I0C3 Sequence was last modified on  February 15, 2005 (version 1) Annotations were last modified on  November 16, 2011 (version 61) Name and origin of the protein Description RecName: Full=Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial; Short=MCCase subunit alpha; EC=6.4.1.4; AltName: Full=3-methylcrotonyl-CoA carboxylase 1; AltName: Full=3-methylcrotonyl-CoA carboxylase biotin-containing subunit; AltName: Full=3-methylcrotonyl-CoA:carbon dioxide ligase subunit alpha; Flags: Precursor; Gene name Name=Mccc1 Synonyms=Mcca Encoded on Name=Mccc1; Synonyms=Mcca Keywords ATP-binding; Biotin; Complete proteome; Ligase; Mitochondrion; Nucleotide-binding; Reference proteome; Transit peptide. Copyright Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License Cross-references EMBL BC088473; AAH88473.1; -; mRNA IPI IPI00372191; -; . RefSeq NP_001009653.1; NM_001009653.1; . UniGene Rn.15587; -; . HSSP P24182; 1BNC; . ProteinModelPortal Q5I0C3; -; . SMR Q5I0C3; 641-714; . STRING Q5I0C3; -; . PhosphoSite Q5I0C3; -; . PRIDE Q5I0C3; -; . GeneID 294972; -; . KEGG rno:294972; -; . UCSC NM_001009653; rat; . CTD 56922; -; . RGD 1310615; Mccc1; . eggNOG roNOG07266; -; . GeneTree ENSGT00550000074675; -; . HOVERGEN HBG000555; -; . InParanoid Q5I0C3; -; . OrthoDB EOG4WQ123; -; . NextBio 638797; -; . ArrayExpress Q5I0C3; -; . Genevestigator Q5I0C3; -; . GO GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell; . GO GO:0005524; F:ATP binding; IEA:UniProtKB-KW; . GO GO:0004075; F:biotin carboxylase activity; IEA:InterPro; . GO GO:0046872; F:metal ion binding; IEA:InterPro; . GO GO:0004485; F:methylcrotonoyl-CoA carboxylase activity; IEA:EC; . InterPro IPR011761; ATP-grasp; . InterPro IPR013815; ATP_grasp_subdomain_1; . InterPro IPR013816; ATP_grasp_subdomain_2; . InterPro IPR001882; Biotin_BS; . InterPro IPR011764; Biotin_carboxylation_dom; . InterPro IPR005482; Biotin_COase_C; . InterPro IPR000089; Biotin_lipoyl; . InterPro IPR005479; CarbamoylP_synth_lsu_ATP-bd; . InterPro IPR005481; CarbamoylP_synth_lsu_N; . InterPro IPR013817; Pre-ATP_grasp; . InterPro IPR016185; PreATP-grasp-like; . InterPro IPR011054; Rudment_hybrid_motif; . InterPro IPR011053; Single_hybrid_motif; . Gene3D G3DSA:3.30.1490.20; ATP_grasp_subdomain_1; 1; . Gene3D G3DSA:3.30.470.20; ATP_grasp_subdomain_2; 1; . Gene3D G3DSA:3.40.50.20; Pre-ATP_grasp; 1; . KO K01968; -; . Pfam PF02785; Biotin_carb_C; 1; . Pfam PF00364; Biotin_lipoyl; 1; . Pfam PF00289; CPSase_L_chain; 1; . Pfam PF02786; CPSase_L_D2; 1; . SMART SM00878; Biotin_carb_C; 1; . SUPFAM SSF51230; Hybrid_motif; 1; . SUPFAM SSF52440; PreATP-grasp-like; 1; . SUPFAM SSF51246; Rudmnt_hyb_motif; 1; . PROSITE PS50975; ATP_GRASP; 1; . PROSITE PS50979; BC; 1; . PROSITE PS00188; BIOTIN; 1; . PROSITE PS50968; BIOTINYL_LIPOYL; 1; . PROSITE PS00866; CPSASE_1; FALSE_NEG; . PROSITE PS00867; CPSASE_2; 1; . World-2DPAGE Repository (search AC)