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Searching in 'World-2DPAGE Repository [0022]' for entry matching: Q5I0C3




World-2DPAGE Repository (0022):  Q5I0C3

Q5I0C3


General information about the entry
View entry in simple text format
Entry nameMCCA_RAT
Primary accession numberQ5I0C3
integrated into World-2DPAGE Repository (0022) on May 7, 2010 (release 1)
2D Annotations were last modified onJune 22, 2011 (version 2)
General Annotations were last modified on November 24, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial; Short=MCCase subunit alpha; EC=6.4.1.4; AltName: Full=3-methylcrotonyl-CoA carboxylase 1; AltName: Full=3-methylcrotonyl-CoA carboxylase biotin-containing subunit; AltName: Full=3-methylcrotonyl-CoA:carbon dioxide ligase subunit alpha; Flags: Precursor;.
Gene nameName=Mccc1
Synonyms=Mcca
Annotated speciesRattus [TaxID: 10114]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000593;
Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L.
''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity''
Proteomics 11(16):3369-3379 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

HCR_LCR_HEART {HCR LCR Cardiac Map}
Rattus
Tissue: Heart
HCR_LCR_HEART
  map experimental info 		 
HCR_LCR_HEART

MAP LOCATIONS:
pI=6.66; Mw=79564  [identification data]
pI=6.66; Mw=79564  [identification data]
pI=6.66; Mw=79564  [identification data]

IDENTIFICATION: SPOT 53: SeqCov=21.7%. Peptides MS=10/20. Mascot PMF Score=72 [1]
SPOT 854: SeqCov=14.4%. Peptides MS=15/20. Mascot PMF Score=37 [1]; SPOT 882: SeqCov=26.3%. Peptides MS=16/20. Mascot PMF Score=133 [1].
PEPTIDE EVIDENCE: SPOT 53: 524-LHTQDQFSPFSFSSGR-539 (107) / 364-IPLSQEEIPLQGHAFEAR-381 (105) / 251-HVEVQVFGDHHGNAVYLFER-270 (87) [1]
SPOT 854: 251-HVEVQVFGDHHGNAVYLFER-270 (56) [1].
MAPPING (identification):
SPOT 53: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 854: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 882: Peptide mass fingerprinting [1].

Copyright
Data from Dr. Jatin Burniston, Liverpool John Moores University, UK
Cross-references
UniProtKB/Swiss-ProtQ5I0C3; MCCA_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameMCCA_RAT
Primary accession numberQ5I0C3
Sequence was last modified on February 15, 2005 (version 1)
Annotations were last modified on November 16, 2011 (version 61)
Name and origin of the protein
DescriptionRecName: Full=Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial; Short=MCCase subunit alpha; EC=6.4.1.4; AltName: Full=3-methylcrotonyl-CoA carboxylase 1; AltName: Full=3-methylcrotonyl-CoA carboxylase biotin-containing subunit; AltName: Full=3-methylcrotonyl-CoA:carbon dioxide ligase subunit alpha; Flags: Precursor;
Gene nameName=Mccc1
Synonyms=Mcca
Encoded onName=Mccc1; Synonyms=Mcca
KeywordsATP-binding; Biotin; Complete proteome; Ligase; Mitochondrion; Nucleotide-binding; Reference proteome; Transit peptide.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLBC088473; AAH88473.1; -; mRNA
IPIIPI00372191; -; .
RefSeqNP_001009653.1; NM_001009653.1; .
UniGeneRn.15587; -; .
HSSPP24182; 1BNC; .
ProteinModelPortalQ5I0C3; -; .
SMRQ5I0C3; 641-714; .
STRINGQ5I0C3; -; .
PhosphoSiteQ5I0C3; -; .
PRIDEQ5I0C3; -; .
GeneID294972; -; .
KEGGrno:294972; -; .
UCSCNM_001009653; rat; .
CTD56922; -; .
RGD1310615; Mccc1; .
eggNOGroNOG07266; -; .
GeneTreeENSGT00550000074675; -; .
HOVERGENHBG000555; -; .
InParanoidQ5I0C3; -; .
OrthoDBEOG4WQ123; -; .
NextBio638797; -; .
ArrayExpressQ5I0C3; -; .
GenevestigatorQ5I0C3; -; .
GOGO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0004075; F:biotin carboxylase activity; IEA:InterPro; .
GOGO:0046872; F:metal ion binding; IEA:InterPro; .
GOGO:0004485; F:methylcrotonoyl-CoA carboxylase activity; IEA:EC; .
InterProIPR011761; ATP-grasp; .
InterProIPR013815; ATP_grasp_subdomain_1; .
InterProIPR013816; ATP_grasp_subdomain_2; .
InterProIPR001882; Biotin_BS; .
InterProIPR011764; Biotin_carboxylation_dom; .
InterProIPR005482; Biotin_COase_C; .
InterProIPR000089; Biotin_lipoyl; .
InterProIPR005479; CarbamoylP_synth_lsu_ATP-bd; .
InterProIPR005481; CarbamoylP_synth_lsu_N; .
InterProIPR013817; Pre-ATP_grasp; .
InterProIPR016185; PreATP-grasp-like; .
InterProIPR011054; Rudment_hybrid_motif; .
InterProIPR011053; Single_hybrid_motif; .
Gene3DG3DSA:3.30.1490.20; ATP_grasp_subdomain_1; 1; .
Gene3DG3DSA:3.30.470.20; ATP_grasp_subdomain_2; 1; .
Gene3DG3DSA:3.40.50.20; Pre-ATP_grasp; 1; .
KOK01968; -; .
PfamPF02785; Biotin_carb_C; 1; .
PfamPF00364; Biotin_lipoyl; 1; .
PfamPF00289; CPSase_L_chain; 1; .
PfamPF02786; CPSase_L_D2; 1; .
SMARTSM00878; Biotin_carb_C; 1; .
SUPFAMSSF51230; Hybrid_motif; 1; .
SUPFAMSSF52440; PreATP-grasp-like; 1; .
SUPFAMSSF51246; Rudmnt_hyb_motif; 1; .
PROSITEPS50975; ATP_GRASP; 1; .
PROSITEPS50979; BC; 1; .
PROSITEPS00188; BIOTIN; 1; .
PROSITEPS50968; BIOTINYL_LIPOYL; 1; .
PROSITEPS00866; CPSASE_1; FALSE_NEG; .
PROSITEPS00867; CPSASE_2; 1; .


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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server