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World-2DPAGE Repository  Search by  [accession number]  [description, ID or gene]  [author names]  [spot ID / serial number]  [identification methods]  [pI / Mw range]  [combined fields]  Maps  [experimental info]  [protein list]  [graphical interface]      Databases 0001 0002 0003 0004 0005 0006 0007 0008 0009 0010 0011 0012 0014 0017 0018 0019 0020 0021 0022 0023 0024 0026 0027 0029 0030 0031 0032 0034 0035 0036 0037 0038 0039 0040 0042 0043 0044 0046 0047 0050 0051 0053 0054 0056 0059 0061 0062 0063 0066 0068 0069 0076 0077 0080 0084 0085 0088 use 'Ctrl' to select several databases Select Remote Interfaces [All Interfaces] SWISS-2DPAGE World-2DPAGE Portal Exclude local DBs has only effect if a remote interface is selected

Searching in 'World-2DPAGE Repository [0030]' for entry matching: Q5U300 World-2DPAGE Repository (0030):  Q5U300 Q5U300 General information about the entry View entry in simple text format Entry name UBA1_RAT Primary accession number Q5U300 integrated into World-2DPAGE Repository (0030) on  November 9, 2010 (release 1) 2D Annotations were last modified on June 21, 2011 (version 2) General Annotations were last modified on  November 23, 2011 (version 2) Name and origin of the protein Description RecName: Full=Ubiquitin-like modifier-activating enzyme 1; AltName: Full=Ubiquitin-activating enzyme E1;. Gene name Name=Uba1 Synonyms=Ube1 Annotated species Rattus norvegicus (Rat) [TaxID: 10116] Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus. References [1]   2D-PAGE GEL CHARACTERIZATION DOI=10.1002/pmic.201000006; D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C. ''Two-dimensional gel proteome reference map of INS-1E cells'' Proteomics 11(7):1365-1369 (2011) 2D PAGE maps for identified proteins How to interpret a protein RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7} Rattus norvegicus (Rat)   map experimental info   RAT_INS1E_4-7 MAP LOCATIONS: SPOT 201: pI=5.33; Mw=98278   [identification data] SPOT 206: pI=5.22; Mw=98143   [identification data] SPOT 207: pI=5.26; Mw=98007   [identification data] IDENTIFICATION: SPOT 201: Mascot protein score=31. Number of peptides=2 [1] SPOT 206: Mascot protein score=117. Number of peptides=3 [1]; SPOT 207: Mascot protein score=71. Number of peptides=3 [1]. MAPPING (identification): SPOT 201: Tandem mass spectrometry [1]; SPOT 206: Tandem mass spectrometry [1]; SPOT 207: Tandem mass spectrometry [1]. Copyright Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium Cross-references UniProtKB/Swiss-Prot Q5U300; UBA1_RAT. World-2DPAGE Repository Q5U300; UBA1_RAT. 2D PAGE maps for identified proteins How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed. External data extracted from UniProtKB/Swiss-Prot Extracted from UniProtKB/Swiss-Prot, release: 2011_11 Entry name UBA1_RAT Primary accession number Q5U300 Sequence was last modified on  December 7, 2004 (version 1) Annotations were last modified on  November 16, 2011 (version 70) Name and origin of the protein Description RecName: Full=Ubiquitin-like modifier-activating enzyme 1; AltName: Full=Ubiquitin-activating enzyme E1; Gene name Name=Uba1 Synonyms=Ube1 Encoded on Name=Uba1; Synonyms=Ube1 Keywords Acetylation; ATP-binding; Complete proteome; Ligase; Nucleotide-binding; Phosphoprotein; Reference proteome; Repeat; Ubl conjugation; Ubl conjugation pathway. Copyright Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License Cross-references EMBL CH474009; EDL97701.1; -; Genomic_DNA EMBL CH474009; EDL97702.1; -; Genomic_DNA EMBL BC085791; AAH85791.1; -; mRNA IPI IPI00368347; -; . RefSeq NP_001014102.1; NM_001014080.1; . UniGene Rn.11800; -; . HSSP Q02053; 1Z7L; . ProteinModelPortal Q5U300; -; . SMR Q5U300; 48-1057; . STRING Q5U300; -; . PhosphoSite Q5U300; -; . PRIDE Q5U300; -; . Ensembl ENSRNOT00000031115; ENSRNOP00000033950; ENSRNOG00000019164; . GeneID 314432; -; . KEGG rno:314432; -; . UCSC NM_001014080; rat; . CTD 7317; -; . RGD 1359327; Uba1; . eggNOG roNOG14955; -; . GeneTree ENSGT00390000016689; -; . HOVERGEN HBG054199; -; . InParanoid Q5U300; -; . OMA ANGMAKN; -; . OrthoDB EOG4QZ7K4; -; . PhylomeDB Q5U300; -; . NextBio 667673; -; . ArrayExpress Q5U300; -; . Genevestigator Q5U300; -; . GO GO:0005524; F:ATP binding; IEA:UniProtKB-KW; . GO GO:0016874; F:ligase activity; IEA:UniProtKB-KW; . GO GO:0008641; F:small protein activating enzyme activity; IEA:InterPro; . GO GO:0006464; P:protein modification process; IEA:InterPro; . InterPro IPR009036; Molybdenum_cofac_synth_MoeB; . InterPro IPR016040; NAD(P)-bd_dom; . InterPro IPR000594; ThiF_NAD_FAD-bd; . InterPro IPR018965; Ub-activating_enz_e1_C; . InterPro IPR023280; Ub-like_act_enz_cat_cys_dom; . InterPro IPR000127; UBact_repeat; . InterPro IPR019572; Ubiquitin-activating_enzyme; . InterPro IPR018075; UBQ-activ_enz_E1; . InterPro IPR018074; UBQ-activ_enz_E1_AS; . InterPro IPR000011; UBQ/SUMO-activ_enz_E1-like; . Gene3D G3DSA:3.40.50.720; NAD(P)-bd; 4; . Gene3D G3DSA:1.10.3240.10; Ub-like_act_enz_cat_cys_dom; 1; . KO K03178; -; . Pfam PF00899; ThiF; 2; . Pfam PF09358; UBA_e1_C; 1; . Pfam PF10585; UBA_e1_thiolCys; 1; . Pfam PF02134; UBACT; 2; . PRINTS PR01849; UBIQUITINACT; . SUPFAM SSF69572; MoeB; 2; . TIGRFAMs TIGR01408; Ube1; 1; . PROSITE PS00536; UBIQUITIN_ACTIVAT_1; 1; . PROSITE PS00865; UBIQUITIN_ACTIVAT_2; 1; . World-2DPAGE Repository (search AC)