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Searching in 'World-2DPAGE Repository [0030]' for entry matching: Q5U300




World-2DPAGE Repository (0030):  Q5U300

Q5U300


General information about the entry
View entry in simple text format
Entry nameUBA1_RAT
Primary accession numberQ5U300
integrated into World-2DPAGE Repository (0030) on November 9, 2010 (release 1)
2D Annotations were last modified onJune 21, 2011 (version 2)
General Annotations were last modified on November 23, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Ubiquitin-like modifier-activating enzyme 1; AltName: Full=Ubiquitin-activating enzyme E1;.
Gene nameName=Uba1
Synonyms=Ube1
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000006;
D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C.
''Two-dimensional gel proteome reference map of INS-1E cells''
Proteomics 11(7):1365-1369 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7}
Rattus norvegicus (Rat)
RAT_INS1E_4-7
  map experimental info 		 
RAT_INS1E_4-7

MAP LOCATIONS:
pI=5.33; Mw=98278  [identification data]
pI=5.22; Mw=98143  [identification data]
pI=5.26; Mw=98007  [identification data]

IDENTIFICATION: SPOT 201: Mascot protein score=31. Number of peptides=2 [1]
SPOT 206: Mascot protein score=117. Number of peptides=3 [1]; SPOT 207: Mascot protein score=71. Number of peptides=3 [1].
MAPPING (identification):
SPOT 201: Tandem mass spectrometry [1];
SPOT 206: Tandem mass spectrometry [1];
SPOT 207: Tandem mass spectrometry [1].

Copyright
Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium
Cross-references
UniProtKB/Swiss-ProtQ5U300; UBA1_RAT.
World-2DPAGE RepositoryQ5U300; UBA1_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameUBA1_RAT
Primary accession numberQ5U300
Sequence was last modified on December 7, 2004 (version 1)
Annotations were last modified on November 16, 2011 (version 70)
Name and origin of the protein
DescriptionRecName: Full=Ubiquitin-like modifier-activating enzyme 1; AltName: Full=Ubiquitin-activating enzyme E1;
Gene nameName=Uba1
Synonyms=Ube1
Encoded onName=Uba1; Synonyms=Ube1
KeywordsAcetylation; ATP-binding; Complete proteome; Ligase; Nucleotide-binding; Phosphoprotein; Reference proteome; Repeat; Ubl conjugation; Ubl conjugation pathway.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLCH474009; EDL97701.1; -; Genomic_DNA
EMBLCH474009; EDL97702.1; -; Genomic_DNA
EMBLBC085791; AAH85791.1; -; mRNA
IPIIPI00368347; -; .
RefSeqNP_001014102.1; NM_001014080.1; .
UniGeneRn.11800; -; .
HSSPQ02053; 1Z7L; .
ProteinModelPortalQ5U300; -; .
SMRQ5U300; 48-1057; .
STRINGQ5U300; -; .
PhosphoSiteQ5U300; -; .
PRIDEQ5U300; -; .
EnsemblENSRNOT00000031115; ENSRNOP00000033950; ENSRNOG00000019164; .
GeneID314432; -; .
KEGGrno:314432; -; .
UCSCNM_001014080; rat; .
CTD7317; -; .
RGD1359327; Uba1; .
eggNOGroNOG14955; -; .
GeneTreeENSGT00390000016689; -; .
HOVERGENHBG054199; -; .
InParanoidQ5U300; -; .
OMAANGMAKN; -; .
OrthoDBEOG4QZ7K4; -; .
PhylomeDBQ5U300; -; .
NextBio667673; -; .
ArrayExpressQ5U300; -; .
GenevestigatorQ5U300; -; .
GOGO:0005524; F:ATP binding; IEA:UniProtKB-KW; .
GOGO:0016874; F:ligase activity; IEA:UniProtKB-KW; .
GOGO:0008641; F:small protein activating enzyme activity; IEA:InterPro; .
GOGO:0006464; P:protein modification process; IEA:InterPro; .
InterProIPR009036; Molybdenum_cofac_synth_MoeB; .
InterProIPR016040; NAD(P)-bd_dom; .
InterProIPR000594; ThiF_NAD_FAD-bd; .
InterProIPR018965; Ub-activating_enz_e1_C; .
InterProIPR023280; Ub-like_act_enz_cat_cys_dom; .
InterProIPR000127; UBact_repeat; .
InterProIPR019572; Ubiquitin-activating_enzyme; .
InterProIPR018075; UBQ-activ_enz_E1; .
InterProIPR018074; UBQ-activ_enz_E1_AS; .
InterProIPR000011; UBQ/SUMO-activ_enz_E1-like; .
Gene3DG3DSA:3.40.50.720; NAD(P)-bd; 4; .
Gene3DG3DSA:1.10.3240.10; Ub-like_act_enz_cat_cys_dom; 1; .
KOK03178; -; .
PfamPF00899; ThiF; 2; .
PfamPF09358; UBA_e1_C; 1; .
PfamPF10585; UBA_e1_thiolCys; 1; .
PfamPF02134; UBACT; 2; .
PRINTSPR01849; UBIQUITINACT; .
SUPFAMSSF69572; MoeB; 2; .
TIGRFAMsTIGR01408; Ube1; 1; .
PROSITEPS00536; UBIQUITIN_ACTIVAT_1; 1; .
PROSITEPS00865; UBIQUITIN_ACTIVAT_2; 1; .


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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server