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Searching in 'World-2DPAGE Repository [0022]' for entry matching: Q5XI78




World-2DPAGE Repository (0022):  Q5XI78


Q5XI78


General information about the entry
View entry in simple text format
Entry nameODO1_RAT
Primary accession numberQ5XI78
integrated into World-2DPAGE Repository (0022) on May 7, 2010 (release 1)
2D Annotations were last modified onJune 22, 2011 (version 2)
General Annotations were last modified on November 24, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=2-oxoglutarate dehydrogenase, mitochondrial; EC=1.2.4.2; AltName: Full=2-oxoglutarate dehydrogenase complex component E1; Short=OGDC-E1; AltName: Full=Alpha-ketoglutarate dehydrogenase; Flags: Precursor;.
Gene nameName=Ogdh
Synonyms=Ogdhl
Annotated speciesRattus [TaxID: 10114]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000593;
Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L.
''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity''
Proteomics 11(16):3369-3379 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

HCR_LCR_HEART {HCR LCR Cardiac Map}
Rattus
Tissue: Heart
HCR_LCR_HEART
  map experimental info
 
HCR_LCR_HEART

MAP LOCATIONS:
pI=6.30; Mw=117419  [identification data]
pI=6.30; Mw=117419  [identification data]
pI=6.30; Mw=117419  [identification data]
pI=6.30; Mw=117419  [identification data]
pI=6.30; Mw=117419  [identification data]
pI=6.30; Mw=117419  [identification data]
pI=6.30; Mw=117419  [identification data]

IDENTIFICATION: SPOT 70: SeqCov=16.4%. Peptides MS=18/20. Mascot PMF Score=141 [1]
SPOT 291: SeqCov=9.5%. Peptides MS=10/20. Mascot PMF Score=41 [1]; SPOT 516: SeqCov=13.5%. Peptides MS=14/20. Mascot PMF Score=83 [1]; SPOT 532: SeqCov=16.5%. Peptides MS=17/20. Mascot PMF Score=126 [1]; SPOT 657: SeqCov=11.1%. Peptides MS=10/20. Mascot PMF Score=48 [1]; SPOT 903: SeqCov=12.1%. Peptides MS=10/20. Mascot PMF Score=52 [1]; SPOT 925: SeqCov=13.9%. Peptides MS=13/20. Mascot PMF Score=87 [1].
PEPTIDE EVIDENCE: SPOT 291: 264-FEEFLQR-270 (15) / 500-DVVVDLVCYR-509 (34) / 185-VFHLPTTTFIGGQEPALPLR-204 (40) [1]
SPOT 516: 264-FEEFLQR-270 (24) [1]; SPOT 657: 75-SWDIFFR-81 (48) / 930-IEQLSPFPFDLLLK-943 (40) / 583-HWLDSPWPGFFTLDGQPR-600 (39) [1]; SPOT 903: 75-SWDIFFR-81 (26) / 583-HWLDSPWPGFFTLDGQPR-600 (20) / 185-VFHLPTTTFIGGQEPALPLR-204 (18) [1]; SPOT 925: 75-SWDIFFR-81 (38) [1].
MAPPING (identification):
SPOT 70: Peptide mass fingerprinting [1];
SPOT 291: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 516: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 532: Peptide mass fingerprinting [1];
SPOT 657: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 903: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 925: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright
Data from Dr. Jatin Burniston, Liverpool John Moores University, UK
Cross-references
UniProtKB/Swiss-ProtQ5XI78; ODO1_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameODO1_RAT
Primary accession numberQ5XI78
Sequence was last modified on November 23, 2004 (version 1)
Annotations were last modified on November 16, 2011 (version 59)
Name and origin of the protein
DescriptionRecName: Full=2-oxoglutarate dehydrogenase, mitochondrial; EC=1.2.4.2; AltName: Full=2-oxoglutarate dehydrogenase complex component E1; Short=OGDC-E1; AltName: Full=Alpha-ketoglutarate dehydrogenase; Flags: Precursor;
Gene nameName=Ogdh
Synonyms=Ogdhl
Encoded onName=Ogdh; Synonyms=Ogdhl
KeywordsAcetylation; Complete proteome; Direct protein sequencing; Glycolysis; Isopeptide bond; Mitochondrion; Oxidoreductase; Reference proteome; Thiamine pyrophosphate; Transit peptide; Ubl conjugation.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLBC083811; AAH83811.1; -; mRNA
IPIIPI00215093; -; .
RefSeqNP_001017461.1; NM_001017461.1; .
UniGeneRn.45991; -; .
ProteinModelPortalQ5XI78; -; .
STRINGQ5XI78; -; .
World-2DPAGE0004:Q5XI78; -; .
PRIDEQ5XI78; -; .
EnsemblENSRNOT00000057199; ENSRNOP00000054026; ENSRNOG00000005130; .
GeneID360975; -; .
KEGGrno:360975; -; .
NMPDRfig|10116.3.peg.10710; -; .
UCSCNM_001017461; rat; .
CTD4967; -; .
RGD1561359; Ogdh; .
eggNOGroNOG15522; -; .
GeneTreeENSGT00530000063092; -; .
HOVERGENHBG001892; -; .
PhylomeDBQ5XI78; -; .
NextBio674779; -; .
ArrayExpressQ5XI78; -; .
GenevestigatorQ5XI78; -; .
GOGO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell; .
GOGO:0031966; C:mitochondrial membrane; ISS:UniProtKB; .
GOGO:0045252; C:oxoglutarate dehydrogenase complex; IDA:RGD; .
GOGO:0051087; F:chaperone binding; IPI:RGD; .
GOGO:0031072; F:heat shock protein binding; IPI:RGD; .
GOGO:0004591; F:oxoglutarate dehydrogenase (succinyl-transferring) activity; ISS:UniProtKB; .
GOGO:0030976; F:thiamine pyrophosphate binding; IEA:InterPro; .
GOGO:0006103; P:2-oxoglutarate metabolic process; IDA:RGD; .
GOGO:0006096; P:glycolysis; IEA:UniProtKB-KW; .
GOGO:0006734; P:NADH metabolic process; IDA:RGD; .
GOGO:0006104; P:succinyl-CoA metabolic process; IDA:RGD; .
GOGO:0006099; P:tricarboxylic acid cycle; IDA:RGD; .
InterProIPR011603; 2oxoglutarate_DH_E1; .
InterProIPR001017; DH_E1; .
InterProIPR005475; Transketolase-like_Pyr-bd; .
KOK00164; -; .
PANTHERPTHR23152; 2oxoglutarate_DH_E1; 1; .
PfamPF00676; E1_dh; 1; .
PfamPF02779; Transket_pyr; 1; .
PIRSFPIRSF000157; Oxoglu_dh_E1; 1; .
SMARTSM00861; Transket_pyr; 1; .
TIGRFAMsTIGR00239; 2oxo_dh_E1; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server