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World-2DPAGE Repository

Attention: World-2DPAGE is no longer maintained.
It will be discontinued on 31-May-2024.

Swiss-2DPAGE data (text records and image files) will continue to be available from https://ftp.expasy.org/databases/swiss-2dpage/.

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Searching in 'World-2DPAGE Repository [0027]' for entry matching: Q64727




World-2DPAGE Repository (0027):  Q64727


Q64727


General information about the entry
View entry in simple text format
Entry nameVINC_MOUSE
Primary accession numberQ64727
integrated into World-2DPAGE Repository (0027) on September 30, 2010 (release 1)
2D Annotations were last modified onJune 21, 2011 (version 2)
General Annotations were last modified on November 23, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Vinculin; AltName: Full=Metavinculin;.
Gene nameName=Vcl
Annotated speciesMus musculus (Mouse) [TaxID: 10090]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus; Mus.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/prca.201000103;
Blutke A., Block C., Berendt F., Herbach N., Kemter E., Amann K., Frolich T., Arnold G.J., Wanke R.
''Differential glomerular proteome analysis of two murine nephropathy models at onset of albuminuria''
Proteomics - Clinical Applications 5(6):375-381 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

GH_4-7 {glomerular protein lysates of a GH-tg animal}
Mus musculus (Mouse)
GH_4-7
  map experimental info
 
GH_4-7

MAP LOCATIONS:
pI=5.90; Mw=115000  [identification data]

EXPRESSION:
SPOT 1: Avg ratio GH-tg vs. control=1.51 (p-value=0.0087) [1].

IDENTIFICATION: SPOT 1: SeqCov=38%. Mascot protein score=343 [1].
MAPPING (identification):
SPOT 1: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright
Data from Dr. Andreas Blutke, Institute of Veterinary Pathology, University Munich, Germany
Cross-references
REPRODUCTION-2DPAGEQ64727; Q64727.
UniProtKB/Swiss-ProtQ64727; VINC_MOUSE.
World-2DPAGE RepositoryQ64727; VINC_MOUSE.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameVINC_MOUSE
Primary accession numberQ64727
Secondary accession number(s) Q8BP32 Q8BS46 Q922C5 Q922D9
Sequence was last modified on January 23, 2007 (version 4)
Annotations were last modified on November 16, 2011 (version 108)
Name and origin of the protein
DescriptionRecName: Full=Vinculin; AltName: Full=Metavinculin;
Gene nameName=Vcl
Encoded onName=Vcl
KeywordsAcetylation; Actin-binding; Cell adhesion; Cell junction; Cell membrane; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Lipoprotein; Membrane; Myristate; Palmitate; Phosphoprotein; Reference proteome; Repeat.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLL13300; AAA40557.1; -; Genomic_DNA
EMBLL13299; AAA40557.1; JOINED; Genomic_DNA
EMBLL18880; AAB96843.1; -; mRNA
EMBLAK035184; BAC28973.1; -; mRNA
EMBLAK077850; BAC37033.1; -; mRNA
EMBLBC008520; AAH08520.1; -; mRNA
EMBLBC008554; AAH08554.1; -; mRNA
IPIIPI00405227; -; .
PIRA60965; A60965; .
PIRT10108; T10108; .
RefSeqNP_033528.3; NM_009502.4; .
UniGeneMm.279361; -; .
ProteinModelPortalQ64727; -; .
SMRQ64727; 1-258; 882-1063; .
IntActQ64727; 2; .
MINTMINT-1634466; -; .
STRINGQ64727; -; .
PhosphoSiteQ64727; -; .
REPRODUCTION-2DPAGEQ64727; -; .
PRIDEQ64727; -; .
EnsemblENSMUST00000022369; ENSMUSP00000022369; ENSMUSG00000021823; .
GeneID22330; -; .
KEGGmmu:22330; -; .
UCSCuc007skz.1; mouse; .
CTD7414; -; .
MGIMGI:98927; Vcl; .
eggNOGroNOG04310; -; .
GeneTreeENSGT00550000074411; -; .
HOGENOMHBG358349; -; .
HOVERGENHBG079758; -; .
InParanoidQ64727; -; .
OMAELTHQVH; -; .
OrthoDBEOG4P5K8C; -; .
NextBio302571; -; .
PMAP-CutDBQ64727; -; .
ArrayExpressQ64727; -; .
BgeeQ64727; -; .
CleanExMM_VCL; -; .
GenevestigatorQ64727; -; .
GermOnlineENSMUSG00000021823; Mus musculus; .
GOGO:0043034; C:costamere; IDA:MGI; .
GOGO:0005916; C:fascia adherens; IDA:MGI; .
GOGO:0005925; C:focal adhesion; IDA:UniProtKB; .
GOGO:0003779; F:actin binding; IEA:UniProtKB-KW; .
GOGO:0005198; F:structural molecule activity; IEA:InterPro; .
GOGO:0030032; P:lamellipodium assembly; IDA:MGI; .
GOGO:0030334; P:regulation of cell migration; IDA:MGI; .
InterProIPR017997; Vinculin; .
InterProIPR006077; Vinculin/catenin; .
InterProIPR000633; Vinculin_CS; .
KOK05700; -; .
PfamPF01044; Vinculin; 2; .
PRINTSPR00806; VINCULIN; .
SUPFAMSSF47220; Vinculin/catenin; 7; .
PROSITEPS00663; VINCULIN_1; 1; .
PROSITEPS00664; VINCULIN_2; 3; .



World-2DPAGE Repository image

World-2DPAGE Repository (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server