World-2DPAGE Home
Home  |  Contact
Due to scheduled maintenance work, this service will not be available from Tuesday August 23rd 06:00 pm until Wednesday August 24th 08:00 am CEST. Apologies for the inconvenience.

World-2DPAGE Repository 
Search by  [accession number] *
[description, ID or gene] 
[author names] 
[spot ID / serial number] 
[identification methods] 
[pI / Mw range] 
[combined fields] 
Maps  [experimental info] 
[protein list] 
[graphical interface]
     Databases

use 'Ctrl' to select several
databases

Select Remote Interfaces
[All Interfaces]
SWISS-2DPAGE
World-2DPAGE Portal
Exclude local DBs
has only effect if a remote
interface is selected
     
Searching in 'World-2DPAGE Repository [0022]' for entry matching: Q66HF1




World-2DPAGE Repository (0022):  Q66HF1

Q66HF1


General information about the entry
View entry in simple text format
Entry nameNDUS1_RAT
Primary accession numberQ66HF1
integrated into World-2DPAGE Repository (0022) on May 7, 2010 (release 1)
2D Annotations were last modified onJune 22, 2011 (version 2)
General Annotations were last modified on November 24, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial; EC=1.6.5.3; EC=1.6.99.3; Flags: Precursor;.
Gene nameName=Ndufs1
Annotated speciesRattus [TaxID: 10114]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000593;
Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L.
''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity''
Proteomics 11(16):3369-3379 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

HCR_LCR_HEART {HCR LCR Cardiac Map}
Rattus
Tissue: Heart
HCR_LCR_HEART
  map experimental info 		 
HCR_LCR_HEART

MAP LOCATIONS:
pI=5.65; Mw=80331  [identification data]
pI=5.65; Mw=80331  [identification data]

IDENTIFICATION: SPOT 607: SeqCov=30.7%. Peptides MS=18/20. Mascot PMF Score=185 [1]
SPOT 920: SeqCov=24.9%. Peptides MS=14/20. Mascot PMF Score=122 [1].
MAPPING (identification):
SPOT 607: Peptide mass fingerprinting [1];
SPOT 920: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright
Data from Dr. Jatin Burniston, Liverpool John Moores University, UK
Cross-references
UniProtKB/Swiss-ProtQ66HF1; NDUS1_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameNDUS1_RAT
Primary accession numberQ66HF1
Sequence was last modified on October 11, 2004 (version 1)
Annotations were last modified on November 16, 2011 (version 64)
Name and origin of the protein
DescriptionRecName: Full=NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial; EC=1.6.5.3; EC=1.6.99.3; Flags: Precursor;
Gene nameName=Ndufs1
Encoded onName=Ndufs1
Keywords2Fe-2S; 4Fe-4S; Acetylation; Complete proteome; Direct protein sequencing; Electron transport; Iron; Iron-sulfur; Membrane; Metal-binding; Mitochondrion; Mitochondrion inner membrane; NAD; Oxidoreductase; Reference proteome; Respiratory chain; Transit peptide; Transport; Ubiquinone.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLBC081892; AAH81892.1; -; mRNA
IPIIPI00358033; -; .
RefSeqNP_001005550.1; NM_001005550.1; .
UniGeneRn.1467; -; .
HSSPQ56223; 2FUG; .
ProteinModelPortalQ66HF1; -; .
STRINGQ66HF1; -; .
PhosphoSiteQ66HF1; -; .
PRIDEQ66HF1; -; .
EnsemblENSRNOT00000015852; ENSRNOP00000015851; ENSRNOG00000011849; .
GeneID301458; -; .
KEGGrno:301458; -; .
NMPDRfig|10116.3.peg.30141; -; .
UCSCNM_001005550; rat; .
CTD4719; -; .
RGD1359670; Ndufs1; .
eggNOGroNOG09474; -; .
GeneTreeENSGT00390000018768; -; .
HOVERGENHBG003482; -; .
InParanoidQ66HF1; -; .
OMADSDSLCT; -; .
OrthoDBEOG4D26PC; -; .
PhylomeDBQ66HF1; -; .
NextBio648791; -; .
ArrayExpressQ66HF1; -; .
GenevestigatorQ66HF1; -; .
GOGO:0005758; C:mitochondrial intermembrane space; ISS:UniProtKB; .
GOGO:0005747; C:mitochondrial respiratory chain complex I; ISS:UniProtKB; .
GOGO:0051537; F:2 iron; 2 sulfur cluster binding; IEA:UniProtKB-KW
GOGO:0051539; F:4 iron; 4 sulfur cluster binding; IEA:UniProtKB-KW
GOGO:0009055; F:electron carrier activity; IEA:InterPro; .
GOGO:0046872; F:metal ion binding; IEA:UniProtKB-KW; .
GOGO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IBA:RefGenome; .
GOGO:0006915; P:apoptosis; ISS:UniProtKB; .
GOGO:0046034; P:ATP metabolic process; ISS:UniProtKB; .
GOGO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro; .
GOGO:0072593; P:reactive oxygen species metabolic process; ISS:UniProtKB; .
GOGO:0051881; P:regulation of mitochondrial membrane potential; ISS:UniProtKB; .
GOGO:0006810; P:transport; IEA:UniProtKB-KW; .
InterProIPR012675; Beta-grasp_ferredoxin-type; .
InterProIPR001041; Ferredoxin; .
InterProIPR006656; Mopterin_OxRdtase; .
InterProIPR000283; NADH_UbQ_OxRdtase_75kDa_su_CS; .
InterProIPR010228; NADH_UbQ_OxRdtase_Gsu; .
InterProIPR019574; NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd; .
InterProIPR015405; NuoG_C; .
Gene3DG3DSA:3.10.20.30; Ferredoxin_fold; 1; .
KOK03934; -; .
PfamPF09326; DUF1982; 1; .
PfamPF00111; Fer2; 1; .
PfamPF00384; Molybdopterin; 1; .
PfamPF10588; NADH-G_4Fe-4S_3; 1; .
SMARTSM00929; NADH-G_4Fe-4S_3; 1; .
SUPFAMSSF54292; Ferredoxin; 1; .
TIGRFAMsTIGR01973; NuoG; 1; .
PROSITEPS51085; 2FE2S_FER_2; 1; .
PROSITEPS00641; COMPLEX1_75K_1; 1; .
PROSITEPS00642; COMPLEX1_75K_2; 1; .
PROSITEPS00643; COMPLEX1_75K_3; 1; .


World-2DPAGE Repository image

World-2DPAGE Repository (search AC)

Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server