World-2DPAGE Home
Home  |  Contact
Due to maintenance work, this service and ftp://ftp.expasy.org will be unavailable from Tuesday August 23rd 18:00 until Wednesday August 24th 08:00 CEST. Apologies for the inconvenience.

World-2DPAGE Repository 
Search by  [accession number] *
[description, ID or gene] 
[author names] 
[spot ID / serial number] 
[identification methods] 
[pI / Mw range] 
[combined fields] 
Maps  [experimental info] 
[protein list] 
[graphical interface]
     Databases

use 'Ctrl' to select several
databases

Select Remote Interfaces
[All Interfaces]
SWISS-2DPAGE
World-2DPAGE Portal
Exclude local DBs
has only effect if a remote
interface is selected
     
Searching in 'World-2DPAGE Repository [0030]' for entry matching: Q8BHN3




World-2DPAGE Repository (0030):  Q8BHN3

Q8BHN3


General information about the entry
View entry in simple text format
Entry nameGANAB_MOUSE
Primary accession numberQ8BHN3
integrated into World-2DPAGE Repository (0030) on November 9, 2010 (release 1)
2D Annotations were last modified onJune 21, 2011 (version 2)
General Annotations were last modified on November 23, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Neutral alpha-glucosidase AB; EC=3.2.1.84; AltName: Full=Alpha-glucosidase 2; AltName: Full=Glucosidase II subunit alpha; Flags: Precursor;.
Gene nameName=Ganab
Synonyms=G2an, Kiaa0088
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000006;
D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C.
''Two-dimensional gel proteome reference map of INS-1E cells''
Proteomics 11(7):1365-1369 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7}
Rattus norvegicus (Rat)
RAT_INS1E_4-7
  map experimental info 		 
RAT_INS1E_4-7

MAP LOCATIONS:
pI=5.85; Mw=100339  [identification data]
pI=5.85; Mw=96527  [identification data]
pI=5.68; Mw=96127  [identification data]
pI=5.83; Mw=96127  [identification data]
pI=5.49; Mw=67459  [identification data]

IDENTIFICATION: SPOT 156: Mascot protein score=282. Number of peptides=10 [1]
SPOT 220: Mascot protein score=86. Number of peptides=3 [1]; SPOT 228: Mascot protein score=52. Number of peptides=3 [1]; SPOT 234: Mascot protein score=209. Number of peptides=8 [1]; SPOT 3027: Mascot protein score=46. Number of peptides=4 [1].
MAPPING (identification):
SPOT 156: Tandem mass spectrometry [1];
SPOT 220: Tandem mass spectrometry [1];
SPOT 228: Tandem mass spectrometry [1];
SPOT 234: Tandem mass spectrometry [1];
SPOT 3027: Tandem mass spectrometry [1].

Copyright
Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium
Cross-references
UniProtKB/Swiss-ProtQ8BHN3; GANAB_MOUSE.
World-2DPAGE RepositoryQ8BHN3; GANAB_MOUSE.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameGANAB_MOUSE
Primary accession numberQ8BHN3
Secondary accession number(s) O08794 Q149A6 Q80U81 Q9CS53
Sequence was last modified on March 1, 2003 (version 1)
Annotations were last modified on November 16, 2011 (version 76)
Name and origin of the protein
DescriptionRecName: Full=Neutral alpha-glucosidase AB; EC=3.2.1.84; AltName: Full=Alpha-glucosidase 2; AltName: Full=Glucosidase II subunit alpha; Flags: Precursor;
Gene nameName=Ganab
Synonyms=G2an, Kiaa0088
Encoded onName=Ganab; Synonyms=G2an, Kiaa0088
KeywordsAlternative splicing; Complete proteome; Direct protein sequencing; Endoplasmic reticulum; Glycoprotein; Glycosidase; Golgi apparatus; Hydrolase; Reference proteome; Signal.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLU92793; AAC53182.1; -; mRNA
EMBLAK017873; BAB30982.1; -; mRNA
EMBLAK030722; BAC27099.1; -; mRNA
EMBLAK081915; BAC38370.1; -; mRNA
EMBLAK122201; BAC65483.1; ALT_INIT; mRNA
EMBLBC094437; AAH94437.1; -; mRNA
EMBLBC117888; AAI17889.1; -; mRNA
EMBLBC117889; AAI17890.1; -; mRNA
IPIIPI00115679; -; .
IPIIPI00403058; -; .
IPIIPI00421253; -; .
RefSeqNP_032086.1; NM_008060.1; .
UniGeneMm.3196; -; .
ProteinModelPortalQ8BHN3; -; .
SMRQ8BHN3; 242-861; .
IntActQ8BHN3; 1; .
STRINGQ8BHN3; -; .
CAZyGH31; Glycoside Hydrolase Family 31; .
PhosphoSiteQ8BHN3; -; .
PRIDEQ8BHN3; -; .
EnsemblENSMUST00000096246; ENSMUSP00000093965; ENSMUSG00000071650; .
EnsemblENSMUST00000096247; ENSMUSP00000093966; ENSMUSG00000071650; .
GeneID14376; -; .
KEGGmmu:14376; -; .
UCSCuc008gnx.1; mouse; .
UCSCuc012big.1; mouse; .
CTD23193; -; .
MGIMGI:1097667; Ganab; .
GeneTreeENSGT00550000074344; -; .
HOVERGENHBG051683; -; .
OMASYPDFFN; -; .
OrthoDBEOG4Z8XVP; -; .
PhylomeDBQ8BHN3; -; .
NextBio285877; -; .
ArrayExpressQ8BHN3; -; .
BgeeQ8BHN3; -; .
CleanExMM_GANAB; -; .
GenevestigatorQ8BHN3; -; .
GermOnlineENSMUSG00000071650; Mus musculus; .
GOGO:0017177; C:glucosidase II complex; IPI:MGI; .
GOGO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell; .
GOGO:0042470; C:melanosome; IEA:UniProtKB-SubCell; .
GOGO:0030246; F:carbohydrate binding; IEA:InterPro; .
GOGO:0033919; F:glucan 1; 3-alpha-glucosidase activity; IEA:EC
GOGO:0005515; F:protein binding; IPI:MGI; .
GOGO:0006491; P:N-glycan processing; TAS:MGI; .
InterProIPR011013; Glyco_hydro-type_carb-bd; .
InterProIPR000322; Glyco_hydro_31; .
InterProIPR017853; Glycoside_hydrolase_SF; .
KOK05546; -; .
PANTHERPTHR22762; Glyco_hydro_31; 1; .
PfamPF01055; Glyco_hydro_31; 1; .
SUPFAMSSF74650; Gal_mut_like; 1; .
SUPFAMSSF51445; Glyco_hydro_cat; 1; .
PROSITEPS00129; GLYCOSYL_HYDROL_F31_1; 1; .
PROSITEPS00707; GLYCOSYL_HYDROL_F31_2; 1; .


World-2DPAGE Repository image

World-2DPAGE Repository (search AC)

Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server