Attention: World-2DPAGE is no longer maintained. Previously submitted data can still be queried at World-2DPAGE Repository.

OB21DSUB {2-DE gel for Olfactory bulb proteome}
Rattus norvegicus (Rat)
Tissue: Olfactory bulb

OB21DSUB

MAP LOCATIONS:

SPOT 226:

pI=5.98; Mw=68000

SPOT 230:

pI=5.94; Mw=68000

MAPPING (identification):

SPOT 226: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 230: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Purnima Bhargava, Centre for Cellular and Molecular Biology, India

Cross-references

UniProtKB/Swiss-Prot

View entry in simple text format

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry name	DHSA_RAT
Primary accession number	Q920L2
Sequence was last modified on	December 1, 2001 (version 1)
Annotations were last modified on	November 16, 2011 (version 85)
Name and origin of the protein
Description	RecName: Full=Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial; EC=1.3.5.1; AltName: Full=Flavoprotein subunit of complex II; Short=Fp; Flags: Precursor;
Gene name	Name=Sdha
Encoded on	Name=Sdha
Keywords	Acetylation; Complete proteome; Direct protein sequencing; Electron transport; FAD; Flavoprotein; Membrane; Mitochondrion; Mitochondrion inner membrane; Oxidoreductase; Phosphoprotein; Reference proteome; Transit peptide; Transport; Tricarboxylic acid cycle; Tumor suppressor.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	AB072907; BAB69818.1; -; mRNA
IPI	IPI00200659; -; .
RefSeq	NP_569112.1; NM_130428.1; .
UniGene	Rn.101725; -; .
ProteinModelPortal	Q920L2; -; .
SMR	Q920L2; 44-656; .
IntAct	Q920L2; 1; .
STRING	Q920L2; -; .
PhosphoSite	Q920L2; -; .
World-2DPAGE	0004:Q920L2; -; .
PRIDE	Q920L2; -; .
Ensembl	ENSRNOT00000018336; ENSRNOP00000018336; ENSRNOG00000013331; .
GeneID	157074; -; .
KEGG	rno:157074; -; .
NMPDR	fig\|10116.3.peg.337; -; .
UCSC	NM_130428; rat; .
CTD	6389; -; .
RGD	621557; Sdha; .
GeneTree	ENSGT00390000010046; -; .
HOVERGEN	HBG001461; -; .
PhylomeDB	Q920L2; -; .
NextBio	620967; -; .
ArrayExpress	Q920L2; -; .
Genevestigator	Q920L2; -; .
GermOnline	ENSRNOG00000013331; Rattus norvegicus; .
GO	GO:0005749; C:mitochondrial respiratory chain complex II; ISS:UniProtKB; .
GO	GO:0009055; F:electron carrier activity; IEA:InterPro; .
GO	GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro; .
GO	GO:0008177; F:succinate dehydrogenase (ubiquinone) activity; IMP:RGD; .
GO	GO:0022904; P:respiratory electron transport chain; ISS:UniProtKB; .
GO	GO:0006105; P:succinate metabolic process; ISS:UniProtKB; .
GO	GO:0006810; P:transport; IEA:UniProtKB-KW; .
GO	GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW; .
InterPro	IPR003953; FAD_bind2_N; .
InterPro	IPR003952; FRD_SDH_FAD_BS; .
InterPro	IPR015939; Fum_Rdtase/Succ_DH_flav-like_C; .
InterPro	IPR004112; Fum_Rdtase/Succ_DH_flav_C; .
InterPro	IPR011281; Succ_DH_flav_su_fwd; .
InterPro	IPR014006; Succ_Dhase_FrdA_Gneg; .
Gene3D	G3DSA:1.20.58.100; Fum_Rdtase/Succ_DH_flav-like_C; 1; .
KO	K00234; -; .
Pfam	PF00890; FAD_binding_2; 1; .
Pfam	PF02910; Succ_DH_flav_C; 1; .
SUPFAM	SSF46977; Succ_DH_flav_C; 1; .
TIGRFAMs	TIGR01816; SdhA_forward; 1; .
TIGRFAMs	TIGR01812; SdhA_frdA_Gneg; 1; .
PROSITE	PS00504; FRD_SDH_FAD_BINDING; 1; .

Searching in 'World-2DPAGE Repository [0014]' for entry matching: Q920L2

World-2DPAGE Repository (0014): Q920L2

Q920L2

General information about the entry

Entry name

DHSA_RAT

Primary accession number

Q920L2

integrated into World-2DPAGE Repository (0014) on

October 13, 2008 (release 1)

2D Annotations were last modified on

June 1, 2011 (version 3)

General Annotations were last modified on

November 25, 2011 (version 2)

Name and origin of the protein

Description

RecName: Full=Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial; EC=1.3.5.1; AltName: Full=Flavoprotein subunit of complex II; Short=Fp; Flags: Precursor;.

Gene name

Name=Sdha

Annotated species

Rattus norvegicus (Rat) [TaxID: 10116]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	2D-PAGE GEL CHARACTERIZATION DOI=10.1002/pmic.200900680; Maurya D.K., Sundaram C.S., Bhargava P. ''Proteome profile of whole cerebellum of the mature rat'' Proteomics 10(23):4311-4319 (2010)

2D PAGE maps for identified proteins

RAT_CEREBELLUM_21D {Proteome of 21 days old rat cerebellum}
Rattus norvegicus (Rat)
Tissue: Cerebellum

RAT_CEREBELLUM_21D

MAP LOCATIONS:

SPOT 2788:

pI=6.75; Mw=73000

IDENTIFICATION: SPOT 2788: SeqCov=46%. MascotPMF=145 [1].
MAPPING (identification):

SPOT 2788: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Purnima Bhargava, Centre for Cellular and Molecular Biology, India

Cross-references

PRIDE

10017.

UniProtKB/Swiss-Prot

View entry in simple text format

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry name	DHSA_RAT
Primary accession number	Q920L2
Sequence was last modified on	December 1, 2001 (version 1)
Annotations were last modified on	November 16, 2011 (version 85)
Name and origin of the protein
Description	RecName: Full=Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial; EC=1.3.5.1; AltName: Full=Flavoprotein subunit of complex II; Short=Fp; Flags: Precursor;
Gene name	Name=Sdha
Encoded on	Name=Sdha
Keywords	Acetylation; Complete proteome; Direct protein sequencing; Electron transport; FAD; Flavoprotein; Membrane; Mitochondrion; Mitochondrion inner membrane; Oxidoreductase; Phosphoprotein; Reference proteome; Transit peptide; Transport; Tricarboxylic acid cycle; Tumor suppressor.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	AB072907; BAB69818.1; -; mRNA
IPI	IPI00200659; -; .
RefSeq	NP_569112.1; NM_130428.1; .
UniGene	Rn.101725; -; .
ProteinModelPortal	Q920L2; -; .
SMR	Q920L2; 44-656; .
IntAct	Q920L2; 1; .
STRING	Q920L2; -; .
PhosphoSite	Q920L2; -; .
World-2DPAGE	0004:Q920L2; -; .
PRIDE	Q920L2; -; .
Ensembl	ENSRNOT00000018336; ENSRNOP00000018336; ENSRNOG00000013331; .
GeneID	157074; -; .
KEGG	rno:157074; -; .
NMPDR	fig\|10116.3.peg.337; -; .
UCSC	NM_130428; rat; .
CTD	6389; -; .
RGD	621557; Sdha; .
GeneTree	ENSGT00390000010046; -; .
HOVERGEN	HBG001461; -; .
PhylomeDB	Q920L2; -; .
NextBio	620967; -; .
ArrayExpress	Q920L2; -; .
Genevestigator	Q920L2; -; .
GermOnline	ENSRNOG00000013331; Rattus norvegicus; .
GO	GO:0005749; C:mitochondrial respiratory chain complex II; ISS:UniProtKB; .
GO	GO:0009055; F:electron carrier activity; IEA:InterPro; .
GO	GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro; .
GO	GO:0008177; F:succinate dehydrogenase (ubiquinone) activity; IMP:RGD; .
GO	GO:0022904; P:respiratory electron transport chain; ISS:UniProtKB; .
GO	GO:0006105; P:succinate metabolic process; ISS:UniProtKB; .
GO	GO:0006810; P:transport; IEA:UniProtKB-KW; .
GO	GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW; .
InterPro	IPR003953; FAD_bind2_N; .
InterPro	IPR003952; FRD_SDH_FAD_BS; .
InterPro	IPR015939; Fum_Rdtase/Succ_DH_flav-like_C; .
InterPro	IPR004112; Fum_Rdtase/Succ_DH_flav_C; .
InterPro	IPR011281; Succ_DH_flav_su_fwd; .
InterPro	IPR014006; Succ_Dhase_FrdA_Gneg; .
Gene3D	G3DSA:1.20.58.100; Fum_Rdtase/Succ_DH_flav-like_C; 1; .
KO	K00234; -; .
Pfam	PF00890; FAD_binding_2; 1; .
Pfam	PF02910; Succ_DH_flav_C; 1; .
SUPFAM	SSF46977; Succ_DH_flav_C; 1; .
TIGRFAMs	TIGR01816; SdhA_forward; 1; .
TIGRFAMs	TIGR01812; SdhA_frdA_Gneg; 1; .
PROSITE	PS00504; FRD_SDH_FAD_BINDING; 1; .

Searching in 'World-2DPAGE Repository [0019]' for entry matching: Q920L2

World-2DPAGE Repository (0019): Q920L2

Q920L2

General information about the entry

Entry name

DHSA_RAT

Primary accession number

Q920L2

integrated into World-2DPAGE Repository (0019) on

December 9, 2009 (release 1)

2D Annotations were last modified on

May 30, 2011 (version 2)

General Annotations were last modified on

January 13, 2012 (version 2)

Name and origin of the protein

Description

RecName: Full=Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial; EC=1.3.5.1; AltName: Full=Flavoprotein subunit of complex II; Short=Fp; Flags: Precursor;.

Gene name

Name=Sdha

Annotated species

Rattus norvegicus (Rat) [TaxID: 10116]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	2D-PAGE GEL CHARACTERIZATION DOI=10.1111/j.1471-4159.2010.06719.x; VanGuilder H.D., Yan H., Farley J.A., Sonntag W.E., Freeman W.M. ''Aging alters the expression of neurotransmission-regulating proteins in the hippocampal synaptoproteome'' J of Neurochemistry 113(6):1577-1588 (2010)

2D PAGE maps for identified proteins

HIPP_SYNAP {Rat hippocampal synaptoproteome}
Rattus norvegicus (Rat)
Tissue: Hippocampus

HIPP_SYNAP

MAP LOCATIONS:

SPOT 321:

pI=6.75; Mw=72596

IDENTIFICATION: SPOT 321: Peptides number=6. SeqCov=11.4%. MOWSE score=1.77E+4 [1].
MAPPING (identification):

SPOT 321: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Willard Freeman, Penn State College of Medicine, USA

Cross-references

UniProtKB/Swiss-Prot

View entry in simple text format

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry name	DHSA_RAT
Primary accession number	Q920L2
Sequence was last modified on	December 1, 2001 (version 1)
Annotations were last modified on	November 16, 2011 (version 85)
Name and origin of the protein
Description	RecName: Full=Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial; EC=1.3.5.1; AltName: Full=Flavoprotein subunit of complex II; Short=Fp; Flags: Precursor;
Gene name	Name=Sdha
Encoded on	Name=Sdha
Keywords	Acetylation; Complete proteome; Direct protein sequencing; Electron transport; FAD; Flavoprotein; Membrane; Mitochondrion; Mitochondrion inner membrane; Oxidoreductase; Phosphoprotein; Reference proteome; Transit peptide; Transport; Tricarboxylic acid cycle; Tumor suppressor.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	AB072907; BAB69818.1; -; mRNA
IPI	IPI00200659; -; .
RefSeq	NP_569112.1; NM_130428.1; .
UniGene	Rn.101725; -; .
ProteinModelPortal	Q920L2; -; .
SMR	Q920L2; 44-656; .
IntAct	Q920L2; 1; .
STRING	Q920L2; -; .
PhosphoSite	Q920L2; -; .
World-2DPAGE	0004:Q920L2; -; .
PRIDE	Q920L2; -; .
Ensembl	ENSRNOT00000018336; ENSRNOP00000018336; ENSRNOG00000013331; .
GeneID	157074; -; .
KEGG	rno:157074; -; .
NMPDR	fig\|10116.3.peg.337; -; .
UCSC	NM_130428; rat; .
CTD	6389; -; .
RGD	621557; Sdha; .
GeneTree	ENSGT00390000010046; -; .
HOVERGEN	HBG001461; -; .
PhylomeDB	Q920L2; -; .
NextBio	620967; -; .
ArrayExpress	Q920L2; -; .
Genevestigator	Q920L2; -; .
GermOnline	ENSRNOG00000013331; Rattus norvegicus; .
GO	GO:0005749; C:mitochondrial respiratory chain complex II; ISS:UniProtKB; .
GO	GO:0009055; F:electron carrier activity; IEA:InterPro; .
GO	GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro; .
GO	GO:0008177; F:succinate dehydrogenase (ubiquinone) activity; IMP:RGD; .
GO	GO:0022904; P:respiratory electron transport chain; ISS:UniProtKB; .
GO	GO:0006105; P:succinate metabolic process; ISS:UniProtKB; .
GO	GO:0006810; P:transport; IEA:UniProtKB-KW; .
GO	GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW; .
InterPro	IPR003953; FAD_bind2_N; .
InterPro	IPR003952; FRD_SDH_FAD_BS; .
InterPro	IPR015939; Fum_Rdtase/Succ_DH_flav-like_C; .
InterPro	IPR004112; Fum_Rdtase/Succ_DH_flav_C; .
InterPro	IPR011281; Succ_DH_flav_su_fwd; .
InterPro	IPR014006; Succ_Dhase_FrdA_Gneg; .
Gene3D	G3DSA:1.20.58.100; Fum_Rdtase/Succ_DH_flav-like_C; 1; .
KO	K00234; -; .
Pfam	PF00890; FAD_binding_2; 1; .
Pfam	PF02910; Succ_DH_flav_C; 1; .
SUPFAM	SSF46977; Succ_DH_flav_C; 1; .
TIGRFAMs	TIGR01816; SdhA_forward; 1; .
TIGRFAMs	TIGR01812; SdhA_frdA_Gneg; 1; .
PROSITE	PS00504; FRD_SDH_FAD_BINDING; 1; .

Searching in 'World-2DPAGE Repository [0022]' for entry matching: Q920L2

World-2DPAGE Repository (0022): Q920L2

Q920L2

General information about the entry

Entry name

DHSA_RAT

Primary accession number

Q920L2

integrated into World-2DPAGE Repository (0022) on

May 7, 2010 (release 1)

2D Annotations were last modified on

June 22, 2011 (version 2)

General Annotations were last modified on

November 24, 2011 (version 2)

Name and origin of the protein

Description

RecName: Full=Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial; EC=1.3.5.1; AltName: Full=Flavoprotein subunit of complex II; Short=Fp; Flags: Precursor;.

Gene name

Name=Sdha

Annotated species

Rattus [TaxID: 10114]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae.

References

[1]

2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000593;
Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L.
''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity''
Proteomics 11(16):3369-3379 (2011)

2D PAGE maps for identified proteins

HCR_LCR_HEART {HCR LCR Cardiac Map}
Rattus
Tissue: Heart

HCR_LCR_HEART

MAP LOCATIONS:

SPOT 506:

pI=6.75; Mw=72596

SPOT 692:

pI=6.75; Mw=72596

SPOT 771:

pI=6.75; Mw=72596

SPOT 902:

pI=6.75; Mw=72596

IDENTIFICATION: SPOT 506: SeqCov=18%. Peptides MS=8/20. Mascot PMF Score=54 [1]
SPOT 692: SeqCov=21%. Peptides MS=10/20. Mascot PMF Score=80 [1]; SPOT 771: SeqCov=22.3%. Peptides MS=11/20. Mascot PMF Score=91 [1]; SPOT 902: SeqCov=29.7%. Peptides MS=14/20. Mascot PMF Score=136 [1].
PEPTIDE EVIDENCE: SPOT 506: 629-VTLDYRPVIDK-639 (39) / 444-LGANSLLDLVVFGR-457 (50) [1]
SPOT 692: 444-LGANSLLDLVVFGR-457 (110) / 354-DHVYLQLHHLPPEQLATR-371 (50) / 39-VSDAISTQYPVVDHEFDAVVVGAGGAGLR-67 (43) [1]; SPOT 771: 608-KPFAEHWR-615 (36) / 629-VTLDYRPVIDK-639 (36) / 444-LGANSLLDLVVFGR-457 (86) [1].
MAPPING (identification):

SPOT 506: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 692: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 771: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 902: Peptide mass fingerprinting [1].

Copyright

Data from Dr. Jatin Burniston, Liverpool John Moores University, UK

Cross-references

UniProtKB/Swiss-Prot