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Searching in 'World-2DPAGE Repository [0004]' for entry matching: Q99NA5




World-2DPAGE Repository (0004):  Q99NA5

Q99NA5


General information about the entry
View entry in simple text format
Entry nameIDH3A_RAT
Primary accession numberQ99NA5
integrated into World-2DPAGE Repository (0004) on October 13, 2008 (release 1)
2D Annotations were last modified onMay 18, 2011 (version 2)
General Annotations were last modified on December 22, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial; EC=1.1.1.41; AltName: Full=Isocitric dehydrogenase subunit alpha; AltName: Full=NAD(+)-specific ICDH subunit alpha; Flags: Precursor;.
Gene nameName=Idh3a
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
References
[1]   MAPPING ON GEL
PubMed=19343716; DOI=10.1002/pmic.200800664; [NCBI, EBI, Israel, Japan]
Maurya D.K., Sundaram C.S., Bhargava P.
''Proteome profile of the mature rat olfactory bulb''
Proteomics 9(1):2593-2599 (2009)
2D PAGE maps for identified proteins
How to interpret a protein

OB21DSUB {2-DE gel for Olfactory bulb proteome}
Rattus norvegicus (Rat)
Tissue: Olfactory bulb
OB21DSUB
  map experimental info 		 
OB21DSUB

MAP LOCATIONS:
pI=5.54; Mw=41000  [identification data]
pI=5.71; Mw=41000  [identification data]

MAPPING (identification):
SPOT 484: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 489: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright
Data from Dr. Purnima Bhargava, Centre for Cellular and Molecular Biology, India
Cross-references
UniProtKB/Swiss-ProtQ99NA5; IDH3A_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry nameIDH3A_RAT
Primary accession numberQ99NA5
Sequence was last modified on June 1, 2001 (version 1)
Annotations were last modified on November 16, 2011 (version 73)
Name and origin of the protein
DescriptionRecName: Full=Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial; EC=1.1.1.41; AltName: Full=Isocitric dehydrogenase subunit alpha; AltName: Full=NAD(+)-specific ICDH subunit alpha; Flags: Precursor;
Gene nameName=Idh3a
Encoded onName=Idh3a
KeywordsAcetylation; Complete proteome; Direct protein sequencing; Magnesium; Manganese; Metal-binding; Mitochondrion; NAD; Oxidoreductase; Reference proteome; Transit peptide; Tricarboxylic acid cycle.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLAB047541; BAB32675.1; -; mRNA
IPIIPI00198720; -; .
RefSeqNP_446090.1; NM_053638.1; .
UniGeneRn.95104; -; .
ProteinModelPortalQ99NA5; -; .
SMRQ99NA5; 26-362; .
IntActQ99NA5; 1; .
STRINGQ99NA5; -; .
World-2DPAGE0004:Q99NA5; -; .
PRIDEQ99NA5; -; .
GeneID114096; -; .
KEGGrno:114096; -; .
UCSCNM_053638; rat; .
CTD3419; -; .
RGD70889; Idh3a; .
eggNOGroNOG14850; -; .
GeneTreeENSGT00550000074918; -; .
HOVERGENHBG052080; -; .
InParanoidQ99NA5; -; .
OrthoDBEOG4GMTX7; -; .
PhylomeDBQ99NA5; -; .
NextBio618241; -; .
ArrayExpressQ99NA5; -; .
GenevestigatorQ99NA5; -; .
GermOnlineENSRNOG00000010277; Rattus norvegicus; .
GOGO:0005962; C:mitochondrial isocitrate dehydrogenase complex (NAD+); IC:RGD; .
GOGO:0004449; F:isocitrate dehydrogenase (NAD+) activity; IDA:RGD; .
GOGO:0000287; F:magnesium ion binding; IEA:InterPro; .
GOGO:0051287; F:NAD binding; IEA:InterPro; .
GOGO:0006103; P:2-oxoglutarate metabolic process; IDA:RGD; .
GOGO:0006102; P:isocitrate metabolic process; IDA:RGD; .
GOGO:0006734; P:NADH metabolic process; IDA:RGD; .
GOGO:0006099; P:tricarboxylic acid cycle; IDA:RGD; .
InterProIPR019818; IsoCit/isopropylmalate_DH_CS; .
InterProIPR001804; Isocitrate/isopropylmalate_DH; .
InterProIPR004434; Isocitrate_DH_NAD; .
InterProIPR024084; IsoPropMal-DH-like_dom; .
Gene3DG3DSA:3.40.718.10; IDH_IMDH; 1; .
KOK00030; -; .
PANTHERPTHR11835; IDH_IMDH_dimeric; 1; .
PfamPF00180; Iso_dh; 1; .
TIGRFAMsTIGR00175; Mito_nad_idh; 1; .
PROSITEPS00470; IDH_IMDH; 1; .





Searching in 'World-2DPAGE Repository [0014]' for entry matching: Q99NA5




World-2DPAGE Repository (0014):  Q99NA5

Q99NA5


General information about the entry
View entry in simple text format
Entry nameIDH3A_RAT
Primary accession numberQ99NA5
integrated into World-2DPAGE Repository (0014) on October 13, 2008 (release 1)
2D Annotations were last modified onJune 1, 2011 (version 3)
General Annotations were last modified on November 25, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial; EC=1.1.1.41; AltName: Full=Isocitric dehydrogenase subunit alpha; AltName: Full=NAD(+)-specific ICDH subunit alpha; Flags: Precursor;.
Gene nameName=Idh3a
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.200900680;
Maurya D.K., Sundaram C.S., Bhargava P.
''Proteome profile of whole cerebellum of the mature rat''
Proteomics 10(23):4311-4319 (2010)
2D PAGE maps for identified proteins
How to interpret a protein

RAT_CEREBELLUM_21D {Proteome of 21 days old rat cerebellum}
Rattus norvegicus (Rat)
Tissue: Cerebellum
RAT_CEREBELLUM_21D
  map experimental info 		 
RAT_CEREBELLUM_21D

MAP LOCATIONS:
pI=5.89; Mw=40000  [identification data]

IDENTIFICATION: SPOT 2988: SeqCov=42%. MascotPMF=115 [1].
MAPPING (identification):
SPOT 2988: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright
Data from Dr. Purnima Bhargava, Centre for Cellular and Molecular Biology, India
Cross-references
PRIDE10017.
UniProtKB/Swiss-ProtQ99NA5; IDH3A_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameIDH3A_RAT
Primary accession numberQ99NA5
Sequence was last modified on June 1, 2001 (version 1)
Annotations were last modified on November 16, 2011 (version 73)
Name and origin of the protein
DescriptionRecName: Full=Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial; EC=1.1.1.41; AltName: Full=Isocitric dehydrogenase subunit alpha; AltName: Full=NAD(+)-specific ICDH subunit alpha; Flags: Precursor;
Gene nameName=Idh3a
Encoded onName=Idh3a
KeywordsAcetylation; Complete proteome; Direct protein sequencing; Magnesium; Manganese; Metal-binding; Mitochondrion; NAD; Oxidoreductase; Reference proteome; Transit peptide; Tricarboxylic acid cycle.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLAB047541; BAB32675.1; -; mRNA
IPIIPI00198720; -; .
RefSeqNP_446090.1; NM_053638.1; .
UniGeneRn.95104; -; .
ProteinModelPortalQ99NA5; -; .
SMRQ99NA5; 26-362; .
IntActQ99NA5; 1; .
STRINGQ99NA5; -; .
World-2DPAGE0004:Q99NA5; -; .
PRIDEQ99NA5; -; .
GeneID114096; -; .
KEGGrno:114096; -; .
UCSCNM_053638; rat; .
CTD3419; -; .
RGD70889; Idh3a; .
eggNOGroNOG14850; -; .
GeneTreeENSGT00550000074918; -; .
HOVERGENHBG052080; -; .
InParanoidQ99NA5; -; .
OrthoDBEOG4GMTX7; -; .
PhylomeDBQ99NA5; -; .
NextBio618241; -; .
ArrayExpressQ99NA5; -; .
GenevestigatorQ99NA5; -; .
GermOnlineENSRNOG00000010277; Rattus norvegicus; .
GOGO:0005962; C:mitochondrial isocitrate dehydrogenase complex (NAD+); IC:RGD; .
GOGO:0004449; F:isocitrate dehydrogenase (NAD+) activity; IDA:RGD; .
GOGO:0000287; F:magnesium ion binding; IEA:InterPro; .
GOGO:0051287; F:NAD binding; IEA:InterPro; .
GOGO:0006103; P:2-oxoglutarate metabolic process; IDA:RGD; .
GOGO:0006102; P:isocitrate metabolic process; IDA:RGD; .
GOGO:0006734; P:NADH metabolic process; IDA:RGD; .
GOGO:0006099; P:tricarboxylic acid cycle; IDA:RGD; .
InterProIPR019818; IsoCit/isopropylmalate_DH_CS; .
InterProIPR001804; Isocitrate/isopropylmalate_DH; .
InterProIPR004434; Isocitrate_DH_NAD; .
InterProIPR024084; IsoPropMal-DH-like_dom; .
Gene3DG3DSA:3.40.718.10; IDH_IMDH; 1; .
KOK00030; -; .
PANTHERPTHR11835; IDH_IMDH_dimeric; 1; .
PfamPF00180; Iso_dh; 1; .
TIGRFAMsTIGR00175; Mito_nad_idh; 1; .
PROSITEPS00470; IDH_IMDH; 1; .





Searching in 'World-2DPAGE Repository [0019]' for entry matching: Q99NA5




World-2DPAGE Repository (0019):  Q99NA5

Q99NA5


General information about the entry
View entry in simple text format
Entry nameIDH3A_RAT
Primary accession numberQ99NA5
integrated into World-2DPAGE Repository (0019) on December 9, 2009 (release 1)
2D Annotations were last modified onMay 30, 2011 (version 2)
General Annotations were last modified on January 13, 2012 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial; EC=1.1.1.41; AltName: Full=Isocitric dehydrogenase subunit alpha; AltName: Full=NAD(+)-specific ICDH subunit alpha; Flags: Precursor;.
Gene nameName=Idh3a
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1111/j.1471-4159.2010.06719.x;
VanGuilder H.D., Yan H., Farley J.A., Sonntag W.E., Freeman W.M.
''Aging alters the expression of neurotransmission-regulating proteins in the hippocampal synaptoproteome''
J of Neurochemistry 113(6):1577-1588 (2010)
2D PAGE maps for identified proteins
How to interpret a protein

HIPP_SYNAP {Rat hippocampal synaptoproteome}
Rattus norvegicus (Rat)
Tissue: Hippocampus
HIPP_SYNAP
  map experimental info 		 
HIPP_SYNAP

MAP LOCATIONS:
pI=6.47; Mw=40044  [identification data]
pI=6.47; Mw=40044  [identification data]
pI=6.47; Mw=40044  [identification data]
pI=6.47; Mw=40044  [identification data]
pI=6.47; Mw=40044  [identification data]

IDENTIFICATION: SPOT 610: Peptides number=3. SeqCov=8.2%. MOWSE score=1.34E+4 [1]
SPOT 611: Peptides number=8. SeqCov=26.5%. MOWSE score=3.54E+10 [1]; SPOT 612: Peptides number=5. SeqCov=18.6%. MOWSE score=2.87E+6 [1]; SPOT 763: Peptides number=3. SeqCov=10.1%. MOWSE score=8.67E+2 [1]; SPOT 1099: Peptides number=5. SeqCov=13.7%. MOWSE score=8.15E+4 [1].
MAPPING (identification):
SPOT 610: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 611: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 612: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 763: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 1099: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright
Data from Dr. Willard Freeman, Penn State College of Medicine, USA
Cross-references
UniProtKB/Swiss-ProtQ99NA5; IDH3A_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry nameIDH3A_RAT
Primary accession numberQ99NA5
Sequence was last modified on June 1, 2001 (version 1)
Annotations were last modified on November 16, 2011 (version 73)
Name and origin of the protein
DescriptionRecName: Full=Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial; EC=1.1.1.41; AltName: Full=Isocitric dehydrogenase subunit alpha; AltName: Full=NAD(+)-specific ICDH subunit alpha; Flags: Precursor;
Gene nameName=Idh3a
Encoded onName=Idh3a
KeywordsAcetylation; Complete proteome; Direct protein sequencing; Magnesium; Manganese; Metal-binding; Mitochondrion; NAD; Oxidoreductase; Reference proteome; Transit peptide; Tricarboxylic acid cycle.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLAB047541; BAB32675.1; -; mRNA
IPIIPI00198720; -; .
RefSeqNP_446090.1; NM_053638.1; .
UniGeneRn.95104; -; .
ProteinModelPortalQ99NA5; -; .
SMRQ99NA5; 26-362; .
IntActQ99NA5; 1; .
STRINGQ99NA5; -; .
World-2DPAGE0004:Q99NA5; -; .
PRIDEQ99NA5; -; .
GeneID114096; -; .
KEGGrno:114096; -; .
UCSCNM_053638; rat; .
CTD3419; -; .
RGD70889; Idh3a; .
eggNOGroNOG14850; -; .
GeneTreeENSGT00550000074918; -; .
HOVERGENHBG052080; -; .
InParanoidQ99NA5; -; .
OrthoDBEOG4GMTX7; -; .
PhylomeDBQ99NA5; -; .
NextBio618241; -; .
ArrayExpressQ99NA5; -; .
GenevestigatorQ99NA5; -; .
GermOnlineENSRNOG00000010277; Rattus norvegicus; .
GOGO:0005962; C:mitochondrial isocitrate dehydrogenase complex (NAD+); IC:RGD; .
GOGO:0004449; F:isocitrate dehydrogenase (NAD+) activity; IDA:RGD; .
GOGO:0000287; F:magnesium ion binding; IEA:InterPro; .
GOGO:0051287; F:NAD binding; IEA:InterPro; .
GOGO:0006103; P:2-oxoglutarate metabolic process; IDA:RGD; .
GOGO:0006102; P:isocitrate metabolic process; IDA:RGD; .
GOGO:0006734; P:NADH metabolic process; IDA:RGD; .
GOGO:0006099; P:tricarboxylic acid cycle; IDA:RGD; .
InterProIPR019818; IsoCit/isopropylmalate_DH_CS; .
InterProIPR001804; Isocitrate/isopropylmalate_DH; .
InterProIPR004434; Isocitrate_DH_NAD; .
InterProIPR024084; IsoPropMal-DH-like_dom; .
Gene3DG3DSA:3.40.718.10; IDH_IMDH; 1; .
KOK00030; -; .
PANTHERPTHR11835; IDH_IMDH_dimeric; 1; .
PfamPF00180; Iso_dh; 1; .
TIGRFAMsTIGR00175; Mito_nad_idh; 1; .
PROSITEPS00470; IDH_IMDH; 1; .





Searching in 'World-2DPAGE Repository [0022]' for entry matching: Q99NA5




World-2DPAGE Repository (0022):  Q99NA5

Q99NA5


General information about the entry
View entry in simple text format
Entry nameIDH3A_RAT
Primary accession numberQ99NA5
integrated into World-2DPAGE Repository (0022) on May 7, 2010 (release 1)
2D Annotations were last modified onJune 22, 2011 (version 2)
General Annotations were last modified on November 24, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial; EC=1.1.1.41; AltName: Full=Isocitric dehydrogenase subunit alpha; AltName: Full=NAD(+)-specific ICDH subunit alpha; Flags: Precursor;.
Gene nameName=Idh3a
Annotated speciesRattus [TaxID: 10114]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000593;
Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L.
''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity''
Proteomics 11(16):3369-3379 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

HCR_LCR_HEART {HCR LCR Cardiac Map}
Rattus
Tissue: Heart
HCR_LCR_HEART
  map experimental info 		 
HCR_LCR_HEART

MAP LOCATIONS:
pI=6.47; Mw=40044  [identification data]
pI=6.47; Mw=40044  [identification data]
pI=6.47; Mw=40044  [identification data]

IDENTIFICATION: SPOT 157: SeqCov=31.1%. Peptides MS=11/20. Mascot PMF Score=85 [1]
SPOT 425: SeqCov=26.8%. Peptides MS=10/20. Mascot PMF Score=81 [1]; SPOT 569: SeqCov=31.4%. Peptides MS=12/20. Mascot PMF Score=99 [1].
PEPTIDE EVIDENCE: SPOT 157: 179-IAEFAFEYAR-188 (73) / 135-TPYTDVNIVTIR-146 (98) / 117-TFDLYANVRPCVSIEGYK-134 (72) [1]
SPOT 425: 59-APIQWEER-66 (58) / 179-IAEFAFEYAR-188 (70) / 135-TPYTDVNIVTIR-146 (99) [1]; SPOT 569: 59-APIQWEER-66 (47) / 179-IAEFAFEYAR-188 (79) / 135-TPYTDVNIVTIR-146 (63) [1].
MAPPING (identification):
SPOT 157: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 425: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 569: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright
Data from Dr. Jatin Burniston, Liverpool John Moores University, UK
Cross-references
UniProtKB/Swiss-ProtQ99NA5; IDH3A_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameIDH3A_RAT
Primary accession numberQ99NA5
Sequence was last modified on June 1, 2001 (version 1)
Annotations were last modified on November 16, 2011 (version 73)
Name and origin of the protein
DescriptionRecName: Full=Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial; EC=1.1.1.41; AltName: Full=Isocitric dehydrogenase subunit alpha; AltName: Full=NAD(+)-specific ICDH subunit alpha; Flags: Precursor;
Gene nameName=Idh3a
Encoded onName=Idh3a
KeywordsAcetylation; Complete proteome; Direct protein sequencing; Magnesium; Manganese; Metal-binding; Mitochondrion; NAD; Oxidoreductase; Reference proteome; Transit peptide; Tricarboxylic acid cycle.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLAB047541; BAB32675.1; -; mRNA
IPIIPI00198720; -; .
RefSeqNP_446090.1; NM_053638.1; .
UniGeneRn.95104; -; .
ProteinModelPortalQ99NA5; -; .
SMRQ99NA5; 26-362; .
IntActQ99NA5; 1; .
STRINGQ99NA5; -; .
World-2DPAGE0004:Q99NA5; -; .
PRIDEQ99NA5; -; .
GeneID114096; -; .
KEGGrno:114096; -; .
UCSCNM_053638; rat; .
CTD3419; -; .
RGD70889; Idh3a; .
eggNOGroNOG14850; -; .
GeneTreeENSGT00550000074918; -; .
HOVERGENHBG052080; -; .
InParanoidQ99NA5; -; .
OrthoDBEOG4GMTX7; -; .
PhylomeDBQ99NA5; -; .
NextBio618241; -; .
ArrayExpressQ99NA5; -; .
GenevestigatorQ99NA5; -; .
GermOnlineENSRNOG00000010277; Rattus norvegicus; .
GOGO:0005962; C:mitochondrial isocitrate dehydrogenase complex (NAD+); IC:RGD; .
GOGO:0004449; F:isocitrate dehydrogenase (NAD+) activity; IDA:RGD; .
GOGO:0000287; F:magnesium ion binding; IEA:InterPro; .
GOGO:0051287; F:NAD binding; IEA:InterPro; .
GOGO:0006103; P:2-oxoglutarate metabolic process; IDA:RGD; .
GOGO:0006102; P:isocitrate metabolic process; IDA:RGD; .
GOGO:0006734; P:NADH metabolic process; IDA:RGD; .
GOGO:0006099; P:tricarboxylic acid cycle; IDA:RGD; .
InterProIPR019818; IsoCit/isopropylmalate_DH_CS; .
InterProIPR001804; Isocitrate/isopropylmalate_DH; .
InterProIPR004434; Isocitrate_DH_NAD; .
InterProIPR024084; IsoPropMal-DH-like_dom; .
Gene3DG3DSA:3.40.718.10; IDH_IMDH; 1; .
KOK00030; -; .
PANTHERPTHR11835; IDH_IMDH_dimeric; 1; .
PfamPF00180; Iso_dh; 1; .
TIGRFAMsTIGR00175; Mito_nad_idh; 1; .
PROSITEPS00470; IDH_IMDH; 1; .





Searching in 'World-2DPAGE Repository [0030]' for entry matching: Q99NA5




World-2DPAGE Repository (0030):  Q99NA5

Q99NA5


General information about the entry
View entry in simple text format
Entry nameIDH3A_RAT
Primary accession numberQ99NA5
integrated into World-2DPAGE Repository (0030) on November 9, 2010 (release 1)
2D Annotations were last modified onJune 21, 2011 (version 2)
General Annotations were last modified on November 23, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial; EC=1.1.1.41; AltName: Full=Isocitric dehydrogenase subunit alpha; AltName: Full=NAD(+)-specific ICDH subunit alpha; Flags: Precursor;.
Gene nameName=Idh3a
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000006;
D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C.
''Two-dimensional gel proteome reference map of INS-1E cells''
Proteomics 11(7):1365-1369 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7}
Rattus norvegicus (Rat)
RAT_INS1E_4-7
  map experimental info 		 
RAT_INS1E_4-7

MAP LOCATIONS:
pI=5.90; Mw=46241  [identification data]
pI=5.66; Mw=44917  [identification data]

IDENTIFICATION: SPOT 1480: Mascot protein score=247. Number of peptides=4 [1]
SPOT 1532: Mascot protein score=209. Number of peptides=7 [1].
MAPPING (identification):
SPOT 1480: Tandem mass spectrometry [1];
SPOT 1532: Tandem mass spectrometry [1].

Copyright
Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium
Cross-references
UniProtKB/Swiss-ProtQ99NA5; IDH3A_RAT.
World-2DPAGE RepositoryQ99NA5; IDH3A_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameIDH3A_RAT
Primary accession numberQ99NA5
Sequence was last modified on June 1, 2001 (version 1)
Annotations were last modified on November 16, 2011 (version 73)
Name and origin of the protein
DescriptionRecName: Full=Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial; EC=1.1.1.41; AltName: Full=Isocitric dehydrogenase subunit alpha; AltName: Full=NAD(+)-specific ICDH subunit alpha; Flags: Precursor;
Gene nameName=Idh3a
Encoded onName=Idh3a
KeywordsAcetylation; Complete proteome; Direct protein sequencing; Magnesium; Manganese; Metal-binding; Mitochondrion; NAD; Oxidoreductase; Reference proteome; Transit peptide; Tricarboxylic acid cycle.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLAB047541; BAB32675.1; -; mRNA
IPIIPI00198720; -; .
RefSeqNP_446090.1; NM_053638.1; .
UniGeneRn.95104; -; .
ProteinModelPortalQ99NA5; -; .
SMRQ99NA5; 26-362; .
IntActQ99NA5; 1; .
STRINGQ99NA5; -; .
World-2DPAGE0004:Q99NA5; -; .
PRIDEQ99NA5; -; .
GeneID114096; -; .
KEGGrno:114096; -; .
UCSCNM_053638; rat; .
CTD3419; -; .
RGD70889; Idh3a; .
eggNOGroNOG14850; -; .
GeneTreeENSGT00550000074918; -; .
HOVERGENHBG052080; -; .
InParanoidQ99NA5; -; .
OrthoDBEOG4GMTX7; -; .
PhylomeDBQ99NA5; -; .
NextBio618241; -; .
ArrayExpressQ99NA5; -; .
GenevestigatorQ99NA5; -; .
GermOnlineENSRNOG00000010277; Rattus norvegicus; .
GOGO:0005962; C:mitochondrial isocitrate dehydrogenase complex (NAD+); IC:RGD; .
GOGO:0004449; F:isocitrate dehydrogenase (NAD+) activity; IDA:RGD; .
GOGO:0000287; F:magnesium ion binding; IEA:InterPro; .
GOGO:0051287; F:NAD binding; IEA:InterPro; .
GOGO:0006103; P:2-oxoglutarate metabolic process; IDA:RGD; .
GOGO:0006102; P:isocitrate metabolic process; IDA:RGD; .
GOGO:0006734; P:NADH metabolic process; IDA:RGD; .
GOGO:0006099; P:tricarboxylic acid cycle; IDA:RGD; .
InterProIPR019818; IsoCit/isopropylmalate_DH_CS; .
InterProIPR001804; Isocitrate/isopropylmalate_DH; .
InterProIPR004434; Isocitrate_DH_NAD; .
InterProIPR024084; IsoPropMal-DH-like_dom; .
Gene3DG3DSA:3.40.718.10; IDH_IMDH; 1; .
KOK00030; -; .
PANTHERPTHR11835; IDH_IMDH_dimeric; 1; .
PfamPF00180; Iso_dh; 1; .
TIGRFAMsTIGR00175; Mito_nad_idh; 1; .
PROSITEPS00470; IDH_IMDH; 1; .





Searching in 'World-2DPAGE Repository [0001]' for entry matching: Q99NA5




World-2DPAGE Repository (0001):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0001)



Searching in 'World-2DPAGE Repository [0002]' for entry matching: Q99NA5




World-2DPAGE Repository (0002):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0002)



Searching in 'World-2DPAGE Repository [0003]' for entry matching: Q99NA5




World-2DPAGE Repository (0003):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0003)



Searching in 'World-2DPAGE Repository [0005]' for entry matching: Q99NA5




World-2DPAGE Repository (0005):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0005)



Searching in 'World-2DPAGE Repository [0006]' for entry matching: Q99NA5




World-2DPAGE Repository (0006):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0006)



Searching in 'World-2DPAGE Repository [0007]' for entry matching: Q99NA5




World-2DPAGE Repository (0007):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0007)



Searching in 'World-2DPAGE Repository [0008]' for entry matching: Q99NA5




World-2DPAGE Repository (0008):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0008)



Searching in 'World-2DPAGE Repository [0009]' for entry matching: Q99NA5




World-2DPAGE Repository (0009):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0009)



Searching in 'World-2DPAGE Repository [0010]' for entry matching: Q99NA5




World-2DPAGE Repository (0010):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0010)



Searching in 'World-2DPAGE Repository [0011]' for entry matching: Q99NA5




World-2DPAGE Repository (0011):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0011)



Searching in 'World-2DPAGE Repository [0012]' for entry matching: Q99NA5




World-2DPAGE Repository (0012):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0012)



Searching in 'World-2DPAGE Repository [0017]' for entry matching: Q99NA5




World-2DPAGE Repository (0017):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0017)



Searching in 'World-2DPAGE Repository [0018]' for entry matching: Q99NA5




World-2DPAGE Repository (0018):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0018)



Searching in 'World-2DPAGE Repository [0020]' for entry matching: Q99NA5




World-2DPAGE Repository (0020):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0020)



Searching in 'World-2DPAGE Repository [0021]' for entry matching: Q99NA5




World-2DPAGE Repository (0021):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0021)



Searching in 'World-2DPAGE Repository [0023]' for entry matching: Q99NA5




World-2DPAGE Repository (0023):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0023)



Searching in 'World-2DPAGE Repository [0024]' for entry matching: Q99NA5




World-2DPAGE Repository (0024):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0024)



Searching in 'World-2DPAGE Repository [0026]' for entry matching: Q99NA5




World-2DPAGE Repository (0026):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0026)



Searching in 'World-2DPAGE Repository [0027]' for entry matching: Q99NA5




World-2DPAGE Repository (0027):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0027)



Searching in 'World-2DPAGE Repository [0029]' for entry matching: Q99NA5




World-2DPAGE Repository (0029):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0029)



Searching in 'World-2DPAGE Repository [0031]' for entry matching: Q99NA5




World-2DPAGE Repository (0031):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0031)



Searching in 'World-2DPAGE Repository [0032]' for entry matching: Q99NA5




World-2DPAGE Repository (0032):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0032)



Searching in 'World-2DPAGE Repository [0034]' for entry matching: Q99NA5




World-2DPAGE Repository (0034):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0034)



Searching in 'World-2DPAGE Repository [0035]' for entry matching: Q99NA5




World-2DPAGE Repository (0035):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0035)



Searching in 'World-2DPAGE Repository [0036]' for entry matching: Q99NA5




World-2DPAGE Repository (0036):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0036)



Searching in 'World-2DPAGE Repository [0037]' for entry matching: Q99NA5




World-2DPAGE Repository (0037):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0037)



Searching in 'World-2DPAGE Repository [0038]' for entry matching: Q99NA5




World-2DPAGE Repository (0038):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0038)



Searching in 'World-2DPAGE Repository [0039]' for entry matching: Q99NA5




World-2DPAGE Repository (0039):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0039)



Searching in 'World-2DPAGE Repository [0040]' for entry matching: Q99NA5




World-2DPAGE Repository (0040):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0040)



Searching in 'World-2DPAGE Repository [0042]' for entry matching: Q99NA5




World-2DPAGE Repository (0042):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0042)



Searching in 'World-2DPAGE Repository [0043]' for entry matching: Q99NA5




World-2DPAGE Repository (0043):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0043)



Searching in 'World-2DPAGE Repository [0044]' for entry matching: Q99NA5




World-2DPAGE Repository (0044):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0044)



Searching in 'World-2DPAGE Repository [0046]' for entry matching: Q99NA5




World-2DPAGE Repository (0046):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0046)



Searching in 'World-2DPAGE Repository [0047]' for entry matching: Q99NA5




World-2DPAGE Repository (0047):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0047)



Searching in 'World-2DPAGE Repository [0050]' for entry matching: Q99NA5




World-2DPAGE Repository (0050):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0050)



Searching in 'World-2DPAGE Repository [0051]' for entry matching: Q99NA5




World-2DPAGE Repository (0051):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0051)



Searching in 'World-2DPAGE Repository [0053]' for entry matching: Q99NA5




World-2DPAGE Repository (0053):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0053)



Searching in 'World-2DPAGE Repository [0054]' for entry matching: Q99NA5




World-2DPAGE Repository (0054):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0054)



Searching in 'World-2DPAGE Repository [0056]' for entry matching: Q99NA5




World-2DPAGE Repository (0056):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0056)



Searching in 'World-2DPAGE Repository [0059]' for entry matching: Q99NA5




World-2DPAGE Repository (0059):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0059)



Searching in 'World-2DPAGE Repository [0061]' for entry matching: Q99NA5




World-2DPAGE Repository (0061):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0061)



Searching in 'World-2DPAGE Repository [0062]' for entry matching: Q99NA5




World-2DPAGE Repository (0062):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0062)



Searching in 'World-2DPAGE Repository [0063]' for entry matching: Q99NA5




World-2DPAGE Repository (0063):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0063)



Searching in 'World-2DPAGE Repository [0066]' for entry matching: Q99NA5




World-2DPAGE Repository (0066):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0066)



Searching in 'World-2DPAGE Repository [0068]' for entry matching: Q99NA5




World-2DPAGE Repository (0068):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0068)



Searching in 'World-2DPAGE Repository [0069]' for entry matching: Q99NA5




World-2DPAGE Repository (0069):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0069)



Searching in 'World-2DPAGE Repository [0076]' for entry matching: Q99NA5




World-2DPAGE Repository (0076):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0076)



Searching in 'World-2DPAGE Repository [0077]' for entry matching: Q99NA5




World-2DPAGE Repository (0077):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0077)



Searching in 'World-2DPAGE Repository [0080]' for entry matching: Q99NA5




World-2DPAGE Repository (0080):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0080)



Searching in 'World-2DPAGE Repository [0084]' for entry matching: Q99NA5




World-2DPAGE Repository (0084):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0084)



Searching in 'World-2DPAGE Repository [0085]' for entry matching: Q99NA5




World-2DPAGE Repository (0085):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0085)



Searching in 'World-2DPAGE Repository [0088]' for entry matching: Q99NA5




World-2DPAGE Repository (0088):  Q99NA5

This protein does not exist in the current release of World-2DPAGE Repository (0088)



World-2DPAGE Repository image

World-2DPAGE Repository (search AC)

Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server