World-2DPAGE Repository

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	2D-PAGE GEL CHARACTERIZATION DOI=10.1111/j.1471-4159.2010.06719.x; VanGuilder H.D., Yan H., Farley J.A., Sonntag W.E., Freeman W.M. ''Aging alters the expression of neurotransmission-regulating proteins in the hippocampal synaptoproteome'' J of Neurochemistry 113(6):1577-1588 (2010)

2D PAGE maps for identified proteins

How to interpret a protein

HIPP_SYNAP {Rat hippocampal synaptoproteome}
Rattus norvegicus (Rat)
Tissue: Hippocampus

map experimental info

HIPP_SYNAP

MAP LOCATIONS:

SPOT 500:

pI=5.65; Mw=15144

[identification data]

SPOT 550:

pI=5.65; Mw=15144

[identification data]

SPOT 841:

pI=5.65; Mw=15144

[identification data]

IDENTIFICATION: SPOT 500: Peptides number=2. SeqCov=7.7%. MOWSE score=4.17E+2 [1]
SPOT 550: Peptides number=3. SeqCov=13.3%. MOWSE score=2.59E+3 [1]; SPOT 841: Peptides number=2. SeqCov=7.7%. MOWSE score=2.58E+3 [1].
MAPPING (identification):

SPOT 500: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 550: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 841: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Willard Freeman, Penn State College of Medicine, USA

Cross-references

UniProtKB/TrEMBL

Q99NC6; Q99NC6_RAT.

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/TrEMBL
Extracted from UniProtKB/TrEMBL, release: 2011_12
Entry name	Q99NC6_RAT
Primary accession number	Q99NC6
Sequence was last modified on	June 1, 2001 (version 1)
Annotations were last modified on	November 16, 2011 (version 49)
Name and origin of the protein
Description	SubName: Full=Beta-actin FE-3; Flags: Fragment;
Gene name	Name=Actg1 Synonyms=Actb
Encoded on	Name=Actg1; Synonyms=Actb
Keywords	Name=Actg1; Synonyms=Actb.
Cross-references
EMBL	AB028846; BAB40316.1; -; mRNA
IPI	IPI00960010; -; .
UniGene	Rn.94978; -; .
HSSP	P60712; 1HLU; .
ProteinModelPortal	Q99NC6; -; .
STRING	Q99NC6; -; .
PRIDE	Q99NC6; -; .
RGD	1304556; Actg1; .
GeneTree	ENSGT00590000082732; -; .
HOVERGEN	HBG003771; -; .
InParanoid	Q99NC6; -; .
ArrayExpress	Q99NC6; -; .
Genevestigator	Q99NC6; -; .
GO	GO:0005524; F:ATP binding; IEA:InterPro; .
InterPro	IPR004000; Actin-like; .
InterPro	IPR004001; Actin_CS; .
PANTHER	PTHR11937; Actin_like; 1; .
Pfam	PF00022; Actin; 1; .
PROSITE	PS00432; ACTINS_2; 1; .

World-2DPAGE Repository (search AC)

Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server

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