World-2DPAGE Repository

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	MAPPING ON GEL PubMed=19343716; DOI=10.1002/pmic.200800664; [NCBI, EBI, Israel, Japan] Maurya D.K., Sundaram C.S., Bhargava P. ''Proteome profile of the mature rat olfactory bulb'' Proteomics 9(1):2593-2599 (2009)

2D PAGE maps for identified proteins

How to interpret a protein

OB21DSUB {2-DE gel for Olfactory bulb proteome}
Rattus norvegicus (Rat)
Tissue: Olfactory bulb

map experimental info

OB21DSUB

MAP LOCATIONS:

SPOT 659:

pI=5.97; Mw=24000

[identification data]

MAPPING (identification):

SPOT 659: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Purnima Bhargava, Centre for Cellular and Molecular Biology, India

Cross-references

UniProtKB/Swiss-Prot

P61589; RHOA_RAT.

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry name	RHOA_RAT
Primary accession number	P61589
Sequence was last modified on	May 24, 2004 (version 1)
Annotations were last modified on	December 14, 2011 (version 84)
Name and origin of the protein
Description	RecName: Full=Transforming protein RhoA; Flags: Precursor;
Gene name	Name=Rhoa Synonyms=Arha, Arha2
Encoded on	Name=Rhoa; Synonyms=Arha, Arha2
Keywords	Cell cycle; Cell division; Cell membrane; Complete proteome; Cytoplasm; Cytoskeleton; GTP-binding; Lipoprotein; Membrane; Methylation; Nucleotide-binding; Phosphoprotein; Prenylation; Proto-oncogene; Reference proteome; Ubl conjugation.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	AY026068; AAK11717.1; -; mRNA
EMBL	AY026069; AAK11718.1; -; mRNA
EMBL	BC061732; AAH61732.1; -; mRNA
IPI	IPI00201699; -; .
RefSeq	NP_476473.1; NM_057132.3; .
UniGene	Rn.107401; -; .
ProteinModelPortal	P61589; -; .
SMR	P61589; 2-181; .
IntAct	P61589; 1; .
MINT	MINT-4542785; -; .
STRING	P61589; -; .
PhosphoSite	P61589; -; .
World-2DPAGE	0004:P61589; -; .
PRIDE	P61589; -; .
GeneID	117273; -; .
KEGG	rno:117273; -; .
UCSC	AY026068; rat; .
CTD	387; -; .
RGD	619921; Rhoa; .
GeneTree	ENSGT00550000074256; -; .
HOVERGEN	HBG009351; -; .
InParanoid	P61589; -; .
OrthoDB	EOG4G4GRD; -; .
PhylomeDB	P61589; -; .
NextBio	620199; -; .
ArrayExpress	P61589; -; .
Genevestigator	P61589; -; .
GermOnline	ENSRNOG00000012630; Rattus norvegicus; .
GO	GO:0030424; C:axon; IDA:RGD; .
GO	GO:0005938; C:cell cortex; ISS:UniProtKB; .
GO	GO:0032154; C:cleavage furrow; ISS:UniProtKB; .
GO	GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell; .
GO	GO:0005624; C:membrane fraction; IDA:RGD; .
GO	GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell; .
GO	GO:0005625; C:soluble fraction; IDA:RGD; .
GO	GO:0005525; F:GTP binding; TAS:RGD; .
GO	GO:0003924; F:GTPase activity; NAS:RGD; .
GO	GO:0019904; F:protein domain specific binding; IPI:RGD; .
GO	GO:0030036; P:actin cytoskeleton organization; TAS:RGD; .
GO	GO:0051927; P:negative regulation of calcium ion transport via voltage-gated calcium channel activity; IMP:RGD; .
GO	GO:0043124; P:negative regulation of I-kappaB kinase/NF-kappaB cascade; IMP:RGD; .
GO	GO:0045665; P:negative regulation of neuron differentiation; IMP:RGD; .
GO	GO:0048812; P:neuron projection morphogenesis; IEP:RGD; .
GO	GO:0030838; P:positive regulation of actin filament polymerization; IMP:RGD; .
GO	GO:0043280; P:positive regulation of cysteine-type endopeptidase activity involved in apoptotic process; IMP:RGD; .
GO	GO:0045785; P:positive regulation of cell adhesion; IEP:RGD; .
GO	GO:0071777; P:positive regulation of cell cycle cytokinesis; ISS:UniProtKB; .
GO	GO:0030307; P:positive regulation of cell growth; IMP:RGD; .
GO	GO:0030335; P:positive regulation of cell migration; IEP:RGD; .
GO	GO:0043123; P:positive regulation of I-kappaB kinase/NF-kappaB cascade; ISS:UniProtKB; .
GO	GO:0043525; P:positive regulation of neuron apoptosis; IMP:RGD; .
GO	GO:0045727; P:positive regulation of translation; IEP:RGD; .
GO	GO:0045907; P:positive regulation of vasoconstriction; IEP:RGD; .
GO	GO:0050773; P:regulation of dendrite development; IMP:RGD; .
GO	GO:0043200; P:response to amino acid stimulus; IEP:RGD; .
GO	GO:0042493; P:response to drug; IDA:RGD; .
GO	GO:0045471; P:response to ethanol; IEP:RGD; .
GO	GO:0051384; P:response to glucocorticoid stimulus; IEP:RGD; .
GO	GO:0009749; P:response to glucose stimulus; IEP:RGD; .
GO	GO:0001666; P:response to hypoxia; IEP:RGD; .
GO	GO:0009612; P:response to mechanical stimulus; IEP:RGD; .
GO	GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro; .
GO	GO:0031098; P:stress-activated protein kinase signaling cascade; IMP:RGD; .
InterPro	IPR005225; Small_GTP-bd_dom; .
InterPro	IPR001806; Small_GTPase; .
InterPro	IPR003578; Small_GTPase_Rho; .
KO	K04513; -; .
Pfam	PF00071; Ras; 1; .
PRINTS	PR00449; RASTRNSFRMNG; .
SMART	SM00174; RHO; 1; .
TIGRFAMs	TIGR00231; Small_GTP; 1; .
PROSITE	PS51420; RHO; 1; .

World-2DPAGE Repository (search AC)

Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server

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