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Searching in 'World-2DPAGE Repository [0030]' for entry matching: TBA1A_RAT




World-2DPAGE Repository (0030):  TBA1A_RAT

TBA1A_RAT


General information about the entry
View entry in simple text format
Entry nameTBA1A_RAT
Primary accession numberP68370
integrated into World-2DPAGE Repository (0030) on November 9, 2010 (release 1)
2D Annotations were last modified onJune 21, 2011 (version 2)
General Annotations were last modified on November 23, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Tubulin alpha-1A chain; AltName: Full=Alpha-tubulin 1; AltName: Full=Tubulin alpha-1 chain;.
Gene nameName=Tuba1a
Synonyms=Tuba1
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000006;
D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C.
''Two-dimensional gel proteome reference map of INS-1E cells''
Proteomics 11(7):1365-1369 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7}
Rattus norvegicus (Rat)
RAT_INS1E_4-7
  map experimental info 		 
RAT_INS1E_4-7

MAP LOCATIONS:
pI=4.92; Mw=66717  [identification data]
pI=4.94; Mw=66533  [identification data]
pI=4.97; Mw=66349  [identification data]
pI=5.37; Mw=66441  [identification data]
pI=4.97; Mw=64627  [identification data]
pI=4.99; Mw=64896  [identification data]
pI=4.99; Mw=66257  [identification data]

IDENTIFICATION: SPOT 873: Mascot protein score=99. Number of peptides=3 [1]
SPOT 877: Mascot protein score=113. Number of peptides=2 [1]; SPOT 878: Mascot protein score=137. Number of peptides=4 [1]; SPOT 879: Mascot protein score=672. Number of peptides=14 [1]; SPOT 942: Mascot protein score=55. Number of peptides=3 [1]; SPOT 2992: Mascot protein score=171. Number of peptides=6 [1]; SPOT 2994: Mascot protein score=815. Number of peptides=12 [1].
MAPPING (identification):
SPOT 873: Tandem mass spectrometry [1];
SPOT 877: Tandem mass spectrometry [1];
SPOT 878: Tandem mass spectrometry [1];
SPOT 879: Tandem mass spectrometry [1];
SPOT 942: Tandem mass spectrometry [1];
SPOT 2992: Tandem mass spectrometry [1];
SPOT 2994: Tandem mass spectrometry [1].

Copyright
Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium
Cross-references
UniProtKB/Swiss-ProtP68370; TBA1A_RAT.
World-2DPAGE RepositoryP68370; TBA1A_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameTBA1A_RAT
Primary accession numberP68370
Secondary accession number(s) P02551 P05210 P05212 Q6P6G6
Sequence was last modified on July 21, 1986 (version 1)
Annotations were last modified on November 16, 2011 (version 74)
Name and origin of the protein
DescriptionRecName: Full=Tubulin alpha-1A chain; AltName: Full=Alpha-tubulin 1; AltName: Full=Tubulin alpha-1 chain;
Gene nameName=Tuba1a
Synonyms=Tuba1
Encoded onName=Tuba1a; Synonyms=Tuba1
KeywordsAcetylation; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; GTP-binding; Microtubule; Nucleotide-binding; Phosphoprotein; Reference proteome.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLV01227; CAA24537.1; -; mRNA
EMBLJ00798; AAA42306.1; -; Genomic_DNA
EMBLJ00797; AAA42306.1; JOINED; Genomic_DNA
EMBLBC062238; AAH62238.1; -; mRNA
EMBLBC078830; AAH78830.1; -; mRNA
EMBLV01226; CAA24536.1; -; mRNA
IPIIPI00189795; -; .
PIRA92869; UBRTA; .
RefSeqNP_071634.1; NM_022298.1; .
UniGeneRn.224466; -; .
UniGeneRn.99661; -; .
ProteinModelPortalP68370; -; .
SMRP68370; 1-440; .
IntActP68370; 4; .
STRINGP68370; -; .
PhosphoSiteP68370; -; .
PRIDEP68370; -; .
EnsemblENSRNOT00000006322; ENSRNOP00000006322; ENSRNOG00000004559; .
GeneID64158; -; .
KEGGrno:64158; -; .
UCSCNM_022298; rat; .
CTD7846; -; .
RGD619717; Tuba1a; .
eggNOGmaNOG08063; -; .
HOVERGENHBG000089; -; .
InParanoidP68370; -; .
OrthoDBEOG44J2HZ; -; .
PhylomeDBP68370; -; .
ReactomeREACT_115492; Developmental Biology; .
NextBio612798; -; .
ArrayExpressP68370; -; .
GenevestigatorP68370; -; .
GermOnlineENSRNOG00000004559; Rattus norvegicus; .
GOGO:0005737; C:cytoplasm; IEA:UniProtKB-KW; .
GOGO:0005874; C:microtubule; TAS:RGD; .
GOGO:0005525; F:GTP binding; IEA:UniProtKB-KW; .
GOGO:0003924; F:GTPase activity; IEA:InterPro; .
GOGO:0019904; F:protein domain specific binding; IDA:RGD; .
GOGO:0046982; F:protein heterodimerization activity; TAS:RGD; .
GOGO:0005198; F:structural molecule activity; IEA:InterPro; .
GOGO:0007018; P:microtubule-based movement; IEA:InterPro; .
GOGO:0051258; P:protein polymerization; IEA:InterPro; .
InterProIPR002452; Alpha_tubulin; .
InterProIPR008280; Tub_FtsZ_C; .
InterProIPR000217; Tubulin; .
InterProIPR018316; Tubulin/FtsZ_2-layer-sand-dom; .
InterProIPR023123; Tubulin_C; .
InterProIPR017975; Tubulin_CS; .
InterProIPR003008; Tubulin_FtsZ_GTPase; .
Gene3DG3DSA:3.30.1330.20; Tubulin/FtsZ_2-layer-sand-dom; 1; .
Gene3DG3DSA:1.10.287.600; Tubulin_C; 1; .
Gene3DG3DSA:3.40.50.1440; Tubulin_FtsZ; 1; .
KOK07374; -; .
PANTHERPTHR11588; Tubulin; 1; .
PfamPF00091; Tubulin; 1; .
PfamPF03953; Tubulin_C; 1; .
PRINTSPR01162; ALPHATUBULIN; .
PRINTSPR01161; TUBULIN; .
SMARTSM00864; Tubulin; 1; .
SMARTSM00865; Tubulin_C; 1; .
SUPFAMSSF55307; Tub_FtsZ_C; 1; .
SUPFAMSSF52490; Tubulin_FtsZ; 1; .
PROSITEPS00227; TUBULIN; 1; .


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