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Searching in 'World-2DPAGE Repository [0022]' for entry matching: THIL_RAT




World-2DPAGE Repository (0022):  THIL_RAT


THIL_RAT


General information about the entry
View entry in simple text format
Entry nameTHIL_RAT
Primary accession numberP17764
integrated into World-2DPAGE Repository (0022) on May 7, 2010 (release 1)
2D Annotations were last modified onJune 22, 2011 (version 2)
General Annotations were last modified on November 24, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Acetyl-CoA acetyltransferase, mitochondrial; EC=2.3.1.9; AltName: Full=Acetoacetyl-CoA thiolase; Flags: Precursor;.
Gene nameName=Acat1
Annotated speciesRattus [TaxID: 10114]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000593;
Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L.
''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity''
Proteomics 11(16):3369-3379 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

HCR_LCR_HEART {HCR LCR Cardiac Map}
Rattus
Tissue: Heart
HCR_LCR_HEART
  map experimental info
 
HCR_LCR_HEART

MAP LOCATIONS:
pI=8.92; Mw=45009  [identification data]
pI=8.92; Mw=45009  [identification data]

IDENTIFICATION: SPOT 41: SeqCov=40.3%. Peptides MS=13/20. Mascot PMF Score=135 [1]
SPOT 152: SeqCov=21.2%. Peptides MS=6/20. Mascot PMF Score=41 [1].
PEPTIDE EVIDENCE: SPOT 152: 200-KLSISR-205 (15) / 206-EEQDKYAIGSYTR-218 (25) / 85-EVYMGNVIQGGEGQAPTR-102 (37) [1].
MAPPING (identification):
SPOT 41: Peptide mass fingerprinting [1];
SPOT 152: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright
Data from Dr. Jatin Burniston, Liverpool John Moores University, UK
Cross-references
UniProtKB/Swiss-ProtP17764; THIL_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameTHIL_RAT
Primary accession numberP17764
Sequence was last modified on August 1, 1990 (version 1)
Annotations were last modified on November 16, 2011 (version 102)
Name and origin of the protein
DescriptionRecName: Full=Acetyl-CoA acetyltransferase, mitochondrial; EC=2.3.1.9; AltName: Full=Acetoacetyl-CoA thiolase; Flags: Precursor;
Gene nameName=Acat1
Encoded onName=Acat1
KeywordsAcetylation; Acyltransferase; Complete proteome; Direct protein sequencing; Metal-binding; Mitochondrion; Potassium; Reference proteome; Transferase; Transit peptide.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLD00512; BAA00401.1; ALT_SEQ; mRNA
EMBLD13921; BAA03016.1; -; mRNA
IPIIPI00324302; -; .
PIRJU0072; XXRTAC; .
RefSeqNP_058771.1; NM_017075.1; .
UniGeneRn.4054; -; .
ProteinModelPortalP17764; -; .
SMRP17764; 32-424; .
IntActP17764; 2; .
MINTMINT-4588676; -; .
STRINGP17764; -; .
PhosphoSiteP17764; -; .
PRIDEP17764; -; .
EnsemblENSRNOT00000010573; ENSRNOP00000010573; ENSRNOG00000007862; .
GeneID25014; -; .
KEGGrno:25014; -; .
UCSCNM_017075; rat; .
CTD38; -; .
RGD2016; Acat1; .
eggNOGroNOG09735; -; .
GeneTreeENSGT00390000009412; -; .
HOVERGENHBG003112; -; .
InParanoidP17764; -; .
OMAAYAVPKV; -; .
OrthoDBEOG4ZW5B8; -; .
PhylomeDBP17764; -; .
NextBio605101; -; .
ArrayExpressP17764; -; .
GenevestigatorP17764; -; .
GermOnlineENSRNOG00000007862; Rattus norvegicus; .
GOGO:0005759; C:mitochondrial matrix; IDA:RGD; .
GOGO:0003985; F:acetyl-CoA C-acetyltransferase activity; IDA:RGD; .
GOGO:0050662; F:coenzyme binding; IPI:RGD; .
GOGO:0019899; F:enzyme binding; IPI:RGD; .
GOGO:0046872; F:metal ion binding; IEA:UniProtKB-KW; .
GOGO:0042803; F:protein homodimerization activity; IDA:RGD; .
GOGO:0060612; P:adipose tissue development; IEP:RGD; .
GOGO:0007420; P:brain development; IEP:RGD; .
GOGO:0001889; P:liver development; IEP:RGD; .
GOGO:0072229; P:metanephric proximal convoluted tubule development; IEP:RGD; .
GOGO:0051260; P:protein homooligomerization; IDA:RGD; .
GOGO:0009725; P:response to hormone stimulus; IEP:RGD; .
GOGO:0014070; P:response to organic cyclic compound; IEP:RGD; .
GOGO:0042594; P:response to starvation; IEP:RGD; .
InterProIPR002155; Thiolase; .
InterProIPR016039; Thiolase-like; .
InterProIPR016038; Thiolase-like_subgr; .
InterProIPR020615; Thiolase_acyl_enz_int_AS; .
InterProIPR020610; Thiolase_AS; .
InterProIPR020617; Thiolase_C; .
InterProIPR020613; Thiolase_CS; .
InterProIPR020616; Thiolase_N; .
Gene3DG3DSA:3.40.47.10; Thiolase-like_subgr; 4; .
KOK00626; -; .
PANTHERPTHR18919; Thiolase; 1; .
PfamPF02803; Thiolase_C; 1; .
PfamPF00108; Thiolase_N; 1; .
PIRSFPIRSF000429; Ac-CoA_Ac_transf; 1; .
SUPFAMSSF53901; Thiolase-like; 2; .
TIGRFAMsTIGR01930; AcCoA-C-Actrans; 1; .
PROSITEPS00098; THIOLASE_1; 1; .
PROSITEPS00737; THIOLASE_2; 1; .
PROSITEPS00099; THIOLASE_3; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server