World-2DPAGE Repository

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.

References

[1]	MAPPING ON GEL PubMed=19343716; DOI=10.1002/pmic.200800664; [NCBI, EBI, Israel, Japan] Maurya D.K., Sundaram C.S., Bhargava P. ''Proteome profile of the mature rat olfactory bulb'' Proteomics 9(1):2593-2599 (2009)

2D PAGE maps for identified proteins

How to interpret a protein

OB21DSUB {2-DE gel for Olfactory bulb proteome}
Rattus norvegicus (Rat)
Tissue: Olfactory bulb

map experimental info

OB21DSUB

MAP LOCATIONS:

SPOT 218:

pI=7.22; Mw=67000

[identification data]

MAPPING (identification):

SPOT 218: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright

Data from Dr. Purnima Bhargava, Centre for Cellular and Molecular Biology, India

Cross-references

UniProtKB/Swiss-Prot

P50137; TKT_RAT.

2D PAGE maps for identified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry name	TKT_RAT
Primary accession number	P50137
Sequence was last modified on	October 1, 1996 (version 1)
Annotations were last modified on	November 16, 2011 (version 89)
Name and origin of the protein
Description	RecName: Full=Transketolase; Short=TK; EC=2.2.1.1;
Gene name	Name=Tkt
Encoded on	Name=Tkt
Keywords	Acetylation; Calcium; Complete proteome; Direct protein sequencing; Metal-binding; Phosphoprotein; Reference proteome; Thiamine pyrophosphate; Transferase.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBL	U09256; AAA18026.1; ALT_INIT; mRNA
IPI	IPI00231139; -; .
RefSeq	NP_072114.1; NM_022592.1; .
UniGene	Rn.5950; -; .
ProteinModelPortal	P50137; -; .
IntAct	P50137; 1; .
MINT	MINT-4566297; -; .
STRING	P50137; -; .
PhosphoSite	P50137; -; .
World-2DPAGE	0004:P50137; -; .
PRIDE	P50137; -; .
GeneID	64524; -; .
KEGG	rno:64524; -; .
UCSC	NM_022592; rat; .
CTD	7086; -; .
RGD	621036; Tkt; .
eggNOG	maNOG05883; -; .
GeneTree	ENSGT00390000005240; -; .
HOVERGEN	HBG004036; -; .
InParanoid	P50137; -; .
OrthoDB	EOG4R23TG; -; .
NextBio	613342; -; .
ArrayExpress	P50137; -; .
Genevestigator	P50137; -; .
GermOnline	ENSRNOG00000016064; Rattus norvegicus; .
GO	GO:0005789; C:endoplasmic reticulum membrane; IDA:RGD; .
GO	GO:0005792; C:microsome; IDA:RGD; .
GO	GO:0005777; C:peroxisome; IDA:RGD; .
GO	GO:0005625; C:soluble fraction; IDA:RGD; .
GO	GO:0000287; F:magnesium ion binding; IDA:RGD; .
GO	GO:0048029; F:monosaccharide binding; IDA:RGD; .
GO	GO:0030976; F:thiamine pyrophosphate binding; IDA:RGD; .
GO	GO:0004802; F:transketolase activity; IDA:RGD; .
GO	GO:0009052; P:pentose-phosphate shunt; non-oxidative branch; IDA:RGD
GO	GO:0046390; P:ribose phosphate biosynthetic process; IDA:RGD; .
InterPro	IPR009014; Transketo_C/Pyr-ferredox_oxred; .
InterPro	IPR015941; Transketolase-like_C; .
InterPro	IPR005475; Transketolase-like_Pyr-bd; .
InterPro	IPR020826; Transketolase_BS; .
InterPro	IPR005476; Transketolase_C; .
InterPro	IPR005474; Transketolase_N; .
Gene3D	G3DSA:3.40.50.920; Transketo_C_like; 1; .
KO	K00615; -; .
Pfam	PF02779; Transket_pyr; 1; .
Pfam	PF02780; Transketolase_C; 1; .
Pfam	PF00456; Transketolase_N; 1; .
SMART	SM00861; Transket_pyr; 1; .
SUPFAM	SSF52922; Transketo_C_like; 1; .
PROSITE	PS00801; TRANSKETOLASE_1; 1; .
PROSITE	PS00802; TRANSKETOLASE_2; 1; .

World-2DPAGE Repository (search AC)

Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server

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