Gel images were imported into the Delta2D V4.1.1 software (Decodon, Greifswald, Germany). Replicates were grouped and gel images aligned using a group warping strategy. Spot detection was performed on a fusion image consisting of an overlay of all gel pictures. The different time points (5, 15 and 90 min) of both the cytosolic fraction and nuclear fraction were compared to the control gel (0 min). Differences in the intensity of each spot were tested with a two-sided Student's T-test and p<0.05 was considered significant without correction for multiple testing.