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Protein list for World-2DPAGE Repository [0027] GH_4-7 
glomerular protein lysates of a GH-tg animal  {Mus musculus (Mouse)}


(map ID: submission_0027:GH_4-7)
Release: 3 -- 23 November 2011

Gel Preparation (local documents: informatics )


Protein list for gh_4-7   14 identified spots / 13 protein entries

Gene Name Description Accession n° Spot ID Exp pI Exp Mw Mapping Methods Comment Topics References
Alb RecName: Full=Serum albumin; Flags: Precursor; P07724
(ALBU_MOUSE)
14
7
6
5.70
5.60
5.70
38'000
71'000
71'000
{MS/MS, PMF}
{MS/MS, PMF}
{MS/MS, PMF}
identification,expression
identification,expression
identification,expression
1
1
1
Anxa4 RecName: Full=Annexin A4; AltName: Full=Annexin IV; AltName: Full=Annexin-4; P97429
(ANXA4_MOUSE)
16
5.30
36'000
{MS/MS, PMF}
identification,expression
1
Dpysl2 RecName: Full=Dihydropyrimidinase-related protein 2; Short=DRP-2; AltName: Full=Unc-33-like phosphoprotein 2; Short=ULIP-2; O08553
(DPYL2_MOUSE)
11
9
8
6.00
6.10
6.00
63'000
62'000
63'000
{MS/MS, PMF}
{MS/MS, PMF}
{MS/MS, PMF}
identification,expression
identification,expression
identification,expression
1
1
1
Ezr RecName: Full=Ezrin; AltName: Full=Cytovillin; AltName: Full=Villin-2; AltName: Full=p81; P26040
(EZRI_MOUSE)
5
4
6.20
6.10
76'000
76'000
{MS/MS, PMF}
{MS/MS, PMF}
identification,expression
identification,expression
1
1
Flna RecName: Full=Filamin-A; Short=FLN-A; AltName: Full=Actin-binding protein 280; Short=ABP-280; AltName: Full=Alpha-filamin; AltName: Full=Endothelial actin-binding protein; AltName: Full=Filamin-1; AltName: Full=Non-muscle filamin; Q8BTM8
(FLNA_MOUSE)
2
6.30
99'000
{MS/MS, PMF}
identification,expression
1
Gnb4 RecName: Full=Guanine nucleotide-binding protein subunit beta-4; AltName: Full=Transducin beta chain 4; P29387
(GBB4_MOUSE)
14
5.70
38'000
{PMF}
Identification
1
Kctd12 RecName: Full=BTB/POZ domain-containing protein KCTD12; AltName: Full=Pfetin; AltName: Full=Predominantly fetal expressed T1 domain; Q6WVG3
(KCD12_MOUSE)
14
5.70
38'000
{PMF}
Identification
1
Lgals1 RecName: Full=Galectin-1; Short=Gal-1; AltName: Full=14 kDa lectin; AltName: Full=Beta-galactoside-binding lectin L-14-I; AltName: Full=Galaptin; AltName: Full=Lactose-binding lectin 1; AltName: Full=Lectin galactoside-binding soluble 1; AltName: Full=S-Lac lectin 1; P16045
(LEG1_MOUSE)
20
5.00
14'000
{MS/MS, PMF}
identification,expression
1
Msn RecName: Full=Moesin; AltName: Full=Membrane-organizing extension spike protein; P26041
(MOES_MOUSE)
4
5
6.10
6.20
76'000
76'000
{MS/MS, PMF}
{MS/MS, PMF}
Identification
Identification
1
1
Myl9 RecName: Full=Myosin regulatory light polypeptide 9; AltName: Full=Myosin regulatory light chain 2, smooth muscle isoform; AltName: Full=Myosin regulatory light chain 9; Q9CQ19
(MYL9_MOUSE)
19
4.70
22'000
{MS/MS, PMF}
identification,expression
1
Rdx RecName: Full=Radixin; AltName: Full=ESP10; P26043
(RADI_MOUSE)
5
4
6.20
6.10
76'000
76'000
{MS/MS, PMF}
{MS/MS, PMF}
Identification
Identification
1
1
Tpm1 RecName: Full=Tropomyosin alpha-1 chain; AltName: Full=Alpha-tropomyosin; AltName: Full=Tropomyosin-1; P58771
(TPM1_MOUSE)
15
4.50
37'000
{MS/MS, PMF}
identification,expression
1
Vcl RecName: Full=Vinculin; AltName: Full=Metavinculin; Q64727
(VINC_MOUSE)
1
5.90
115'000
{MS/MS, PMF}
identification,expression
1
Mapping legends: { MS/MS } => 'Tandem mass spectrometry', { PMF } => 'Peptide mass fingerprinting'



Cited references

# PubMed/Medline Authors Location
[1] doi:10.1002/prca.201000103 Blutke A., Block C., Berendt F., Herbach N., Kemter E., Amann K., Frolich T., Arnold G.J., Wanke R.; Proteomics - Clinical Applications 5(6):375-381 (2011).
''Differential glomerular proteome analysis of two murine nephropathy models at onset of albuminuria'';


Gel experimental information

Information Type Data
Map name GH_4-7 {glomerular protein lysates of a GH-tg animal}
Dimension 2-D
Related documents Gel Preparation (local documents: informatics )
species Mus musculus (Mouse) / TaxID:10090
pI start: 4.00 - end: 7.00
Mw start: 10000 - end: 200000
Informatics Software DeCyder Differential Analysis Software v6.5
Number of identified spots 14
Number of identified proteins 13
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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server