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Searching in 'World-2DPAGE Repository [0002]' for entry matching: P64028




World-2DPAGE Repository (0002):  P64028


P64028


General information about the entry
View entry in simple text format
Entry nameEFTU_STAAM
Primary accession numberP64028
integrated into World-2DPAGE Repository (0002) on December 7, 2005 (release 1)
2D Annotations were last modified onMay 1, 2011 (version 2)
General Annotations were last modified on November 14, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Elongation factor Tu; Short=EF-Tu;.
Gene nameName=tuf
Synonyms=tufA
OrderedLocusNames=SAV0548
Annotated speciesStaphylococcus aureus (strain Mu50 / ATCC 700699) [TaxID: 158878]
TaxonomyBacteria; Firmicutes; Bacillales; Staphylococcus.
References
[1]   MAPPING ON GEL
PubMed=17370256; DOI=10.1002/pmic.200600925; [NCBI, EBI, Israel, Japan]
Plikat U., Voshol H., Dangendorf Y., Wiedmann B., Devay P., Mueller D., Wirth U., Szustakowski J., Chirn G.W., Inverardi B., Puyang X., Brown K., Kamp H., Hoving S., Ruchti A., Brendlen N., Peterson R., Buco J., Oostrum J., Peitsch M.C.
''From proteomics to systems biology of bacterial pathogens: approaches, tools, and applications''
Proteomics 7(6):992-1003 (2007)
2D PAGE maps for identified proteins
How to interpret a protein

STAPHY4-7 {Staphylococcus aureus, pH 4-7}
Staphylococcus aureus (strain Mu50 / ATCC 700699)
STAPHY4-7
  map experimental info
 
STAPHY4-7

MAP LOCATIONS:
pI=4.93; Mw=54000  [identification data]
pI=4.97; Mw=51000  [identification data]
pI=4.93; Mw=51000  [identification data]
pI=5.00; Mw=51000  [identification data]
pI=4.82; Mw=50000  [identification data]
pI=4.97; Mw=48000  [identification data]
pI=4.82; Mw=47000  [identification data]
pI=4.86; Mw=44000  [identification data]
pI=4.87; Mw=42000  [identification data]
pI=4.77; Mw=38000  [identification data]
pI=4.69; Mw=35000  [identification data]
pI=4.73; Mw=33000  [identification data]
pI=4.69; Mw=33000  [identification data]
pI=4.86; Mw=32000  [identification data]
pI=4.71; Mw=32000  [identification data]
pI=4.77; Mw=32000  [identification data]
pI=4.86; Mw=31000  [identification data]
pI=4.92; Mw=30000  [identification data]
pI=4.75; Mw=30000  [identification data]
pI=4.86; Mw=28000  [identification data]
pI=4.95; Mw=23000  [identification data]
pI=5.33; Mw=22000  [identification data]
pI=5.33; Mw=13000  [identification data]
pI=5.28; Mw=10000  [identification data]
pI=6.14; Mw=9000  [identification data]
pI=4.89; Mw=35000  [identification data]

AREA: SPOT 1137: 581 [1]
SPOT 1192: 3945 [1]; SPOT 1215: 677 [1]; SPOT 1216: 898 [1]; SPOT 1233: 895 [1]; SPOT 1272: 1311 [1]; SPOT 1303: 588 [1]; SPOT 1381: 753 [1]; SPOT 1423: 380 [1]; SPOT 1543: 621 [1]; SPOT 1677: 760 [1]; SPOT 1746: 1030 [1]; SPOT 1762: 897 [1]; SPOT 1799: 1643 [1]; SPOT 1805: 678 [1]; SPOT 1849: 627 [1]; SPOT 1920: 374 [1]; SPOT 1933: 1242 [1]; SPOT 1936: 419 [1]; SPOT 2083: 711 [1]; SPOT 2243: 1643 [1]; SPOT 2261: 344 [1]; SPOT 2445: 2149 [1]; SPOT 2569: 3139 [1]; SPOT 2575: 2460 [1]; SPOT 2920: 1108 [1].
PEAK HEIGHT: SPOT 1137: 12127.291 [1]
SPOT 1192: 35119.985 [1]; SPOT 1215: 14594.329 [1]; SPOT 1216: 20733.5 [1]; SPOT 1233: 15423.552 [1]; SPOT 1272: 25767.975 [1]; SPOT 1303: 8939.24 [1]; SPOT 1381: 9630.048 [1]; SPOT 1423: 11609.869 [1]; SPOT 1543: 9137.826 [1]; SPOT 1677: 8416.232 [1]; SPOT 1746: 10358.253 [1]; SPOT 1762: 9478.245 [1]; SPOT 1799: 15586.787 [1]; SPOT 1805: 7986.424 [1]; SPOT 1849: 8555.759 [1]; SPOT 1920: 2323.023 [1]; SPOT 1933: 12028.086 [1]; SPOT 1936: 2713.346 [1]; SPOT 2083: 3349.786 [1]; SPOT 2243: 9062.841 [1]; SPOT 2261: 9364.333 [1]; SPOT 2445: 12142.297 [1]; SPOT 2569: 10823.462 [1]; SPOT 2575: 9883.253 [1]; SPOT 2920: 14782.783 [1].
STAPH_LSI: SPOT 1137: stapha_lsi_0548 [1]
SPOT 1192: stapha_lsi_0548 [1]; SPOT 1215: stapha_lsi_0548 [1]; SPOT 1216: stapha_lsi_0548 [1]; SPOT 1233: stapha_lsi_0548 [1]; SPOT 1272: stapha_lsi_0548 [1]; SPOT 1303: stapha_lsi_0548 [1]; SPOT 1381: stapha_lsi_0548 [1]; SPOT 1423: stapha_lsi_0548 [1]; SPOT 1543: stapha_lsi_0548 [1]; SPOT 1677: stapha_lsi_0548 [1]; SPOT 1746: stapha_lsi_0548 [1]; SPOT 1762: stapha_lsi_0548 [1]; SPOT 1799: stapha_lsi_0548 [1]; SPOT 1805: stapha_lsi_0548 [1]; SPOT 1849: stapha_lsi_0548 [1]; SPOT 1920: stapha_lsi_0548 [1]; SPOT 1933: stapha_lsi_0548 [1]; SPOT 1936: stapha_lsi_0548 [1]; SPOT 2083: stapha_lsi_0548 [1]; SPOT 2243: stapha_lsi_0548 [1]; SPOT 2261: stapha_lsi_0548 [1]; SPOT 2445: stapha_lsi_0548 [1]; SPOT 2569: stapha_lsi_0548 [1]; SPOT 2575: stapha_lsi_0548 [1]; SPOT 2920: stapha_lsi_0548 [1].
MAPPING (identification):
SPOT 1137: Peptide mass fingerprinting [1];
SPOT 1192: Peptide mass fingerprinting [1];
SPOT 1215: Peptide mass fingerprinting [1];
SPOT 1216: Peptide mass fingerprinting [1];
SPOT 1233: Peptide mass fingerprinting [1];
SPOT 1272: Peptide mass fingerprinting [1];
SPOT 1303: Peptide mass fingerprinting [1];
SPOT 1381: Peptide mass fingerprinting [1];
SPOT 1423: Peptide mass fingerprinting [1];
SPOT 1543: Peptide mass fingerprinting [1];
SPOT 1677: Peptide mass fingerprinting [1];
SPOT 1746: Peptide mass fingerprinting [1];
SPOT 1762: Peptide mass fingerprinting [1];
SPOT 1799: Peptide mass fingerprinting [1];
SPOT 1805: Peptide mass fingerprinting [1];
SPOT 1849: Peptide mass fingerprinting [1];
SPOT 1920: Peptide mass fingerprinting [1];
SPOT 1933: Peptide mass fingerprinting [1];
SPOT 1936: Peptide mass fingerprinting [1];
SPOT 2083: Peptide mass fingerprinting [1];
SPOT 2243: Peptide mass fingerprinting [1];
SPOT 2261: Peptide mass fingerprinting [1];
SPOT 2445: Peptide mass fingerprinting [1];
SPOT 2569: Peptide mass fingerprinting [1];
SPOT 2575: Peptide mass fingerprinting [1];
SPOT 2920: Peptide mass fingerprinting [1].

Copyright
Data from Dr. Hans Voshol, Novartis Institutes for BioMedical Research, Basel, Switzerland
Cross-references
UniProtKB/Swiss-ProtP64028; EFTU_STAAM.
World-2DPAGE RepositoryP64028; EFTU_STAAM.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameEFTU_STAAM
Primary accession numberP64028
Secondary accession number(s) Q99W61
Sequence was last modified on October 11, 2004 (version 1)
Annotations were last modified on October 19, 2011 (version 46)
Name and origin of the protein
DescriptionRecName: Full=Elongation factor Tu; Short=EF-Tu;
Gene nameName=tuf
Synonyms=tufA
OrderedLocusNames=SAV0548
Encoded onName=tuf; Synonyms=tufA; OrderedLocusNames=SAV0548
KeywordsComplete proteome; Cytoplasm; Elongation factor; GTP-binding; Nucleotide-binding; Protein biosynthesis.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLBA000017; BAB56710.1; -; Genomic_DNA
RefSeqNP_371072.1; NC_002758.2; .
ProteinModelPortalP64028; -; .
SMRP64028; 2-393; .
STRINGP64028; -; .
World-2DPAGE0002:P64028; -; .
EnsemblBacteriaEBSTAT00000005791; EBSTAP00000005609; EBSTAG00000005790; .
GeneID1120521; -; .
GenomeReviewsBA000017_GR; SAV0548; .
KEGGsav:SAV0548; -; .
eggNOGCOG0050; -; .
GeneTreeEBGT00050000024902; -; .
HOGENOMHBG307581; -; .
OMAMEKGLRF; -; .
ProtClustDBPRK00049; -; .
BioCycSAUR158878:SAV0548-MONOMER; -; .
GOGO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell; .
GOGO:0005525; F:GTP binding; IEA:UniProtKB-KW; .
GOGO:0003924; F:GTPase activity; IEA:InterPro; .
GOGO:0003746; F:translation elongation factor activity; IEA:UniProtKB-KW; .
HAMAPMF_00118_B; EF_Tu_B; 1; -
InterProIPR000795; ProtSyn_GTP-bd; .
InterProIPR005225; Small_GTP-bd_dom; .
InterProIPR009001; Transl_elong_EF1A/Init_IF2_C; .
InterProIPR004161; Transl_elong_EFTu/EF1A_2; .
InterProIPR004541; Transl_elong_EFTu/EF1A_bac/org; .
InterProIPR004160; Transl_elong_EFTu/EF1A_C; .
InterProIPR009000; Transl_elong_init/rib_B-barrel; .
PANTHERPTHR23115:SF31; Transl_elong_EFTu/EF1A_bac/org; 1; .
PfamPF00009; GTP_EFTU; 1; .
PfamPF03144; GTP_EFTU_D2; 1; .
PfamPF03143; GTP_EFTU_D3; 1; .
PRINTSPR00315; ELONGATNFCT; .
SUPFAMSSF50465; Elong_init_C; 1; .
SUPFAMSSF50447; Translat_factor; 1; .
TIGRFAMsTIGR00485; EF-Tu; 1; .
TIGRFAMsTIGR00231; Small_GTP; 1; .
PROSITEPS00301; EFACTOR_GTP; 1; .



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World-2DPAGE Repository (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server