World-2DPAGE Home
Home  |  Contact

World-2DPAGE Repository 
Search by  [accession number] *
[description, ID or gene] 
[author names] 
[spot ID / serial number] 
[identification methods] 
[pI / Mw range] 
[combined fields] 

Maps  [experimental info] 
[protein list] 
[graphical interface]


use 'Ctrl' to select several

Select Remote Interfaces
[All Interfaces]
World-2DPAGE Portal

Exclude local DBs
has only effect if a remote
interface is selected
Searching in 'World-2DPAGE Repository [0002]' for entry matching: P65645

World-2DPAGE Repository (0002):  P65645


General information about the entry
View entry in simple text format
Entry nameISAA_STAAM
Primary accession numberP65645
integrated into World-2DPAGE Repository (0002) on December 7, 2005 (release 1)
2D Annotations were last modified onMay 1, 2011 (version 2)
General Annotations were last modified on November 14, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Probable transglycosylase isaA; EC=3.2.-.-; AltName: Full=Immunodominant staphylococcal antigen A; Flags: Precursor;.
Gene nameName=isaA
Annotated speciesStaphylococcus aureus (strain Mu50 / ATCC 700699) [TaxID: 158878]
TaxonomyBacteria; Firmicutes; Bacillales; Staphylococcus.
PubMed=17370256; DOI=10.1002/pmic.200600925; [NCBI, EBI, Israel, Japan]
Plikat U., Voshol H., Dangendorf Y., Wiedmann B., Devay P., Mueller D., Wirth U., Szustakowski J., Chirn G.W., Inverardi B., Puyang X., Brown K., Kamp H., Hoving S., Ruchti A., Brendlen N., Peterson R., Buco J., Oostrum J., Peitsch M.C.
''From proteomics to systems biology of bacterial pathogens: approaches, tools, and applications''
Proteomics 7(6):992-1003 (2007)
2D PAGE maps for identified proteins
How to interpret a protein

STAPHY4-7 {Staphylococcus aureus, pH 4-7}
Staphylococcus aureus (strain Mu50 / ATCC 700699)
  map experimental info

pI=5.28; Mw=29000  [identification data]
pI=4.45; Mw=24000  [identification data]
pI=5.68; Mw=30000  [identification data]

AREA: SPOT 2010: 3253 [1]
SPOT 2227: 906 [1]; SPOT 2981: 333 [1].
PEAK HEIGHT: SPOT 2010: 23523.381 [1]
SPOT 2227: 7356.217 [1]; SPOT 2981: 8303.021 [1].
STAPH_LSI: SPOT 2010: stapha_lsi_2569 [1]
SPOT 2227: stapha_lsi_2569 [1]; SPOT 2981: stapha_lsi_2569 [1].
MAPPING (identification):
SPOT 2010: Peptide mass fingerprinting [1];
SPOT 2227: Peptide mass fingerprinting [1];
SPOT 2981: Peptide mass fingerprinting [1].

STAPHY6-11 {Staphylococcus aureus, pH 6-11}
Staphylococcus aureus (strain Mu50 / ATCC 700699)
  map experimental info

pI=9.16; Mw=14000  [identification data]

AREA: SPOT 486: 4577 [1].
PEAK HEIGHT: SPOT 486: 5741 [1].
STAPHA_LSI: SPOT 486: stapha_lsi_2569 [1].
MAPPING (identification):
SPOT 486: Peptide mass fingerprinting [1].

Data from Dr. Hans Voshol, Novartis Institutes for BioMedical Research, Basel, Switzerland
UniProtKB/Swiss-ProtP65645; ISAA_STAAM.
World-2DPAGE RepositoryP65645; ISAA_STAAM.

2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.

External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_10
Entry nameISAA_STAAM
Primary accession numberP65645
Secondary accession number(s) Q99R69
Sequence was last modified on October 11, 2004 (version 1)
Annotations were last modified on October 19, 2011 (version 40)
Name and origin of the protein
DescriptionRecName: Full=Probable transglycosylase isaA; EC=3.2.-.-; AltName: Full=Immunodominant staphylococcal antigen A; Flags: Precursor;
Gene nameName=isaA
Encoded onName=isaA; OrderedLocusNames=SAV2569
KeywordsComplete proteome; Glycosidase; Hydrolase; Secreted; Signal.
Copyrighted by the UniProt Consortium, see Distributed under the Creative Commons Attribution-NoDerivs License
EMBLBA000017; BAB58731.1; -; Genomic_DNA
RefSeqNP_373093.1; NC_002758.2; .
ProteinModelPortalP65645; -; .
STRINGP65645; -; .
World-2DPAGE0002:P65645; -; .
EnsemblBacteriaEBSTAT00000007687; EBSTAP00000007505; EBSTAG00000007686; .
GeneID1122594; -; .
GenomeReviewsBA000017_GR; SAV2569; .
KEGGsav:SAV2569; -; .
GeneTreeEBGT00050000023704; -; .
HOGENOMHBG693910; -; .
ProtClustDBCLSK886077; -; .
BioCycSAUR158878:SAV2569-MONOMER; -; .
GOGO:0005576; C:extracellular region; IEA:UniProtKB-SubCell; .
GOGO:0016798; F:hydrolase activity; acting on glycosyl bonds; IEA:UniProtKB-KW
InterProIPR023346; Lysozyme-like_dom; .
InterProIPR010618; Transglycosylas; .
PfamPF06737; Transglycosylas; 1; .
SUPFAMSSF53955; SSF53955; 1; .

World-2DPAGE Repository image

World-2DPAGE Repository (search AC)

Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server