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Searching in 'World-2DPAGE Repository [0008]' for entry matching: O68897




World-2DPAGE Repository (0008):  O68897


O68897


General information about the entry
View entry in simple text format
Entry nameASPQ_PSEFA
Primary accession numberO68897
integrated into World-2DPAGE Repository (0008) on February 5, 2009 (release 1)
2D Annotations were last modified onMay 27, 2011 (version 2)
General Annotations were last modified on November 23, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Glutaminase-asparaginase; EC=3.5.1.38; AltName: Full=L-ASNase/L-GLNase; AltName: Full=L-asparagine/L-glutamine amidohydrolase; Flags: Precursor;.
Gene nameName=ansB
Annotated speciesPseudomonas putida group [TaxID: 136845]
TaxonomyBacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.200900142;
Cheng Z., Woody O.Z., Song J., Glick B.R., McConkey B.J.
''Proteome reference map for the plant growth-promotiong bacterium Pseudomonas putida UW4''
Proteomics 9(17):4271-4274 (2009)
2D PAGE maps for identified proteins
How to interpret a protein

PSEPU_UW4 {Pseudomonas putida UW4 proteome reference map}
Pseudomonas putida group
PSEPU_UW4
  map experimental info
 
PSEPU_UW4

MAP LOCATIONS:
pI=6.51; Mw=43800  [identification data]
pI=6.74; Mw=40500  [identification data]

IDENTIFICATION COMMENT: SPOT 247: SeqCov=11.6%. Mascot Protein score=68.35 [1]
SPOT 253: SeqCov=23.76%. Mascot Protein score=97.87 [1].
MAPPING (identification):
SPOT 247: Tandem mass spectrometry [1];
SPOT 253: Tandem mass spectrometry [1].

Copyright
Data from Dr. Zhenyu Cheng, University of Waterloo, Canada
Cross-references
UniProtKB/Swiss-ProtO68897; ASPQ_PSEFA.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameASPQ_PSEFA
Primary accession numberO68897
Sequence was last modified on August 1, 1998 (version 1)
Annotations were last modified on July 27, 2011 (version 51)
Name and origin of the protein
DescriptionRecName: Full=Glutaminase-asparaginase; EC=3.5.1.38; AltName: Full=L-ASNase/L-GLNase; AltName: Full=L-asparagine/L-glutamine amidohydrolase; Flags: Precursor;
Gene nameName=ansB
Encoded onName=ansB
KeywordsHydrolase; Periplasm; Signal.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLAF056495; AAC33155.1; -; Genomic_DNA
ProteinModelPortalO68897; -; .
SMRO68897; 33-362; .
World-2DPAGE0008:O68897; -; .
GOGO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell; .
GOGO:0004067; F:asparaginase activity; IEA:InterPro; .
GOGO:0050417; F:glutamin-(asparagin-)ase activity; IEA:EC; .
GOGO:0006528; P:asparagine metabolic process; IEA:InterPro; .
InterProIPR004550; AsnASE_II; .
InterProIPR006034; Asparaginase/glutaminase; .
InterProIPR020827; Asparaginase/glutaminase_CS; .
PANTHERPTHR11707; Asp/Glutamnse; 1; .
PfamPF00710; Asparaginase; 1; .
PIRSFPIRSF001220; L-ASNase_gatD; 1; .
PRINTSPR00139; ASNGLNASE; .
SMARTSM00870; Asparaginase; 1; .
SUPFAMSSF53774; Asp/Glutamnse; 1; .
TIGRFAMsTIGR00520; AsnASE_II; 1; .
PROSITEPS00144; ASN_GLN_ASE_1; 1; .
PROSITEPS00917; ASN_GLN_ASE_2; 1; .



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Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server