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Searching in 'World-2DPAGE Repository [0004]' for entry matching: O88989




World-2DPAGE Repository (0004):  O88989

O88989


General information about the entry
View entry in simple text format
Entry nameMDHC_RAT
Primary accession numberO88989
integrated into World-2DPAGE Repository (0004) on October 13, 2008 (release 1)
2D Annotations were last modified onMay 18, 2011 (version 2)
General Annotations were last modified on December 22, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Malate dehydrogenase, cytoplasmic; EC=1.1.1.37; AltName: Full=Cytosolic malate dehydrogenase;.
Gene nameName=Mdh1
Synonyms=Mdh
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
References
[1]   MAPPING ON GEL
PubMed=19343716; DOI=10.1002/pmic.200800664; [NCBI, EBI, Israel, Japan]
Maurya D.K., Sundaram C.S., Bhargava P.
''Proteome profile of the mature rat olfactory bulb''
Proteomics 9(1):2593-2599 (2009)
2D PAGE maps for identified proteins
How to interpret a protein

OB21DSUB {2-DE gel for Olfactory bulb proteome}
Rattus norvegicus (Rat)
Tissue: Olfactory bulb
OB21DSUB
  map experimental info 		 
OB21DSUB

MAP LOCATIONS:
pI=5.77; Mw=37000  [identification data]
pI=5.92; Mw=37000  [identification data]

MAPPING (identification):
SPOT 527: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 528: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright
Data from Dr. Purnima Bhargava, Centre for Cellular and Molecular Biology, India
Cross-references
UniProtKB/Swiss-ProtO88989; MDHC_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry nameMDHC_RAT
Primary accession numberO88989
Secondary accession number(s) O88585 Q6PCV2
Sequence was last modified on January 23, 2007 (version 3)
Annotations were last modified on November 16, 2011 (version 84)
Name and origin of the protein
DescriptionRecName: Full=Malate dehydrogenase, cytoplasmic; EC=1.1.1.37; AltName: Full=Cytosolic malate dehydrogenase;
Gene nameName=Mdh1
Synonyms=Mdh
Encoded onName=Mdh1; Synonyms=Mdh
KeywordsAcetylation; Complete proteome; Cytoplasm; Direct protein sequencing; NAD; Oxidoreductase; Phosphoprotein; Reference proteome; Tricarboxylic acid cycle; Ubl conjugation.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLAF093773; AAC64180.1; -; mRNA
EMBLBC059124; AAH59124.1; -; mRNA
EMBLAF075574; AAC26799.1; -; mRNA
IPIIPI00198717; -; .
RefSeqNP_150238.1; NM_033235.1; .
UniGeneRn.13492; -; .
ProteinModelPortalO88989; -; .
SMRO88989; 2-334; .
IntActO88989; 1; .
STRINGO88989; -; .
PhosphoSiteO88989; -; .
World-2DPAGE0004:O88989; -; .
PRIDEO88989; -; .
EnsemblENSRNOT00000011429; ENSRNOP00000011429; ENSRNOG00000008103; .
GeneID24551; -; .
KEGGrno:24551; -; .
NMPDRfig|10116.3.peg.10844; -; .
UCSCNM_033235; rat; .
CTD4190; -; .
RGD3072; Mdh1; .
eggNOGroNOG11216; -; .
GeneTreeENSGT00530000063410; -; .
HOVERGENHBG006340; -; .
InParanoidO88989; -; .
OrthoDBEOG4CVG78; -; .
PhylomeDBO88989; -; .
NextBio603654; -; .
ArrayExpressO88989; -; .
GenevestigatorO88989; -; .
GermOnlineENSRNOG00000008103; Rattus norvegicus; .
GOGO:0005625; C:soluble fraction; IDA:RGD; .
GOGO:0030060; F:L-malate dehydrogenase activity; IDA:RGD; .
GOGO:0051287; F:NAD binding; IDA:RGD; .
GOGO:0044262; P:cellular carbohydrate metabolic process; IEA:InterPro; .
GOGO:0006108; P:malate metabolic process; IDA:RGD; .
GOGO:0006734; P:NADH metabolic process; IDA:RGD; .
GOGO:0006107; P:oxaloacetate metabolic process; IDA:RGD; .
GOGO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW; .
InterProIPR001557; L-lactate/malate_DH; .
InterProIPR022383; Lactate/malate_DH_C; .
InterProIPR001236; Lactate/malate_DH_N; .
InterProIPR015955; Lactate_DH/Glyco_Ohase_4_C; .
InterProIPR001252; Malate_DH_AS; .
InterProIPR011274; Malate_DH_NAD-dep_euk; .
InterProIPR010945; Malate_DH_type2; .
InterProIPR016040; NAD(P)-bd_dom; .
Gene3DG3DSA:3.90.110.10; lact_mal_DH; 1; .
Gene3DG3DSA:3.40.50.720; NAD(P)-bd; 1; .
KOK00025; -; .
PANTHERPTHR23382; MDH_SF1; 1; .
PfamPF02866; Ldh_1_C; 1; .
PfamPF00056; Ldh_1_N; 1; .
PIRSFPIRSF000102; Lac_mal_DH; 1; .
SUPFAMSSF56327; Lactate_DH/Glyco_hydro_4_C; 1; .
TIGRFAMsTIGR01759; MalateDH-SF1; 1; .
TIGRFAMsTIGR01758; MDH_euk_cyt; 1; .
PROSITEPS00068; MDH; 1; .





Searching in 'World-2DPAGE Repository [0014]' for entry matching: O88989




World-2DPAGE Repository (0014):  O88989

O88989


General information about the entry
View entry in simple text format
Entry nameMDHC_RAT
Primary accession numberO88989
integrated into World-2DPAGE Repository (0014) on October 13, 2008 (release 1)
2D Annotations were last modified onJune 1, 2011 (version 3)
General Annotations were last modified on November 25, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Malate dehydrogenase, cytoplasmic; EC=1.1.1.37; AltName: Full=Cytosolic malate dehydrogenase;.
Gene nameName=Mdh1
Synonyms=Mdh
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.200900680;
Maurya D.K., Sundaram C.S., Bhargava P.
''Proteome profile of whole cerebellum of the mature rat''
Proteomics 10(23):4311-4319 (2010)
2D PAGE maps for identified proteins
How to interpret a protein

RAT_CEREBELLUM_21D {Proteome of 21 days old rat cerebellum}
Rattus norvegicus (Rat)
Tissue: Cerebellum
RAT_CEREBELLUM_21D
  map experimental info 		 
RAT_CEREBELLUM_21D

MAP LOCATIONS:
pI=6.75; Mw=38000  [identification data]

IDENTIFICATION: SPOT 3028: SeqCov=38%. MascotPMF=73 [1].
MAPPING (identification):
SPOT 3028: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright
Data from Dr. Purnima Bhargava, Centre for Cellular and Molecular Biology, India
Cross-references
PRIDE10017.
UniProtKB/Swiss-ProtO88989; MDHC_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameMDHC_RAT
Primary accession numberO88989
Secondary accession number(s) O88585 Q6PCV2
Sequence was last modified on January 23, 2007 (version 3)
Annotations were last modified on November 16, 2011 (version 84)
Name and origin of the protein
DescriptionRecName: Full=Malate dehydrogenase, cytoplasmic; EC=1.1.1.37; AltName: Full=Cytosolic malate dehydrogenase;
Gene nameName=Mdh1
Synonyms=Mdh
Encoded onName=Mdh1; Synonyms=Mdh
KeywordsAcetylation; Complete proteome; Cytoplasm; Direct protein sequencing; NAD; Oxidoreductase; Phosphoprotein; Reference proteome; Tricarboxylic acid cycle; Ubl conjugation.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLAF093773; AAC64180.1; -; mRNA
EMBLBC059124; AAH59124.1; -; mRNA
EMBLAF075574; AAC26799.1; -; mRNA
IPIIPI00198717; -; .
RefSeqNP_150238.1; NM_033235.1; .
UniGeneRn.13492; -; .
ProteinModelPortalO88989; -; .
SMRO88989; 2-334; .
IntActO88989; 1; .
STRINGO88989; -; .
PhosphoSiteO88989; -; .
World-2DPAGE0004:O88989; -; .
PRIDEO88989; -; .
EnsemblENSRNOT00000011429; ENSRNOP00000011429; ENSRNOG00000008103; .
GeneID24551; -; .
KEGGrno:24551; -; .
NMPDRfig|10116.3.peg.10844; -; .
UCSCNM_033235; rat; .
CTD4190; -; .
RGD3072; Mdh1; .
eggNOGroNOG11216; -; .
GeneTreeENSGT00530000063410; -; .
HOVERGENHBG006340; -; .
InParanoidO88989; -; .
OrthoDBEOG4CVG78; -; .
PhylomeDBO88989; -; .
NextBio603654; -; .
ArrayExpressO88989; -; .
GenevestigatorO88989; -; .
GermOnlineENSRNOG00000008103; Rattus norvegicus; .
GOGO:0005625; C:soluble fraction; IDA:RGD; .
GOGO:0030060; F:L-malate dehydrogenase activity; IDA:RGD; .
GOGO:0051287; F:NAD binding; IDA:RGD; .
GOGO:0044262; P:cellular carbohydrate metabolic process; IEA:InterPro; .
GOGO:0006108; P:malate metabolic process; IDA:RGD; .
GOGO:0006734; P:NADH metabolic process; IDA:RGD; .
GOGO:0006107; P:oxaloacetate metabolic process; IDA:RGD; .
GOGO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW; .
InterProIPR001557; L-lactate/malate_DH; .
InterProIPR022383; Lactate/malate_DH_C; .
InterProIPR001236; Lactate/malate_DH_N; .
InterProIPR015955; Lactate_DH/Glyco_Ohase_4_C; .
InterProIPR001252; Malate_DH_AS; .
InterProIPR011274; Malate_DH_NAD-dep_euk; .
InterProIPR010945; Malate_DH_type2; .
InterProIPR016040; NAD(P)-bd_dom; .
Gene3DG3DSA:3.90.110.10; lact_mal_DH; 1; .
Gene3DG3DSA:3.40.50.720; NAD(P)-bd; 1; .
KOK00025; -; .
PANTHERPTHR23382; MDH_SF1; 1; .
PfamPF02866; Ldh_1_C; 1; .
PfamPF00056; Ldh_1_N; 1; .
PIRSFPIRSF000102; Lac_mal_DH; 1; .
SUPFAMSSF56327; Lactate_DH/Glyco_hydro_4_C; 1; .
TIGRFAMsTIGR01759; MalateDH-SF1; 1; .
TIGRFAMsTIGR01758; MDH_euk_cyt; 1; .
PROSITEPS00068; MDH; 1; .





Searching in 'World-2DPAGE Repository [0019]' for entry matching: O88989




World-2DPAGE Repository (0019):  O88989

O88989


General information about the entry
View entry in simple text format
Entry nameMDHC_RAT
Primary accession numberO88989
integrated into World-2DPAGE Repository (0019) on December 9, 2009 (release 1)
2D Annotations were last modified onMay 30, 2011 (version 2)
General Annotations were last modified on January 13, 2012 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Malate dehydrogenase, cytoplasmic; EC=1.1.1.37; AltName: Full=Cytosolic malate dehydrogenase;.
Gene nameName=Mdh1
Synonyms=Mdh
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1111/j.1471-4159.2010.06719.x;
VanGuilder H.D., Yan H., Farley J.A., Sonntag W.E., Freeman W.M.
''Aging alters the expression of neurotransmission-regulating proteins in the hippocampal synaptoproteome''
J of Neurochemistry 113(6):1577-1588 (2010)
2D PAGE maps for identified proteins
How to interpret a protein

HIPP_SYNAP {Rat hippocampal synaptoproteome}
Rattus norvegicus (Rat)
Tissue: Hippocampus
HIPP_SYNAP
  map experimental info 		 
HIPP_SYNAP

MAP LOCATIONS:
pI=5.93; Mw=36632  [identification data]
pI=5.93; Mw=36632  [identification data]
pI=5.93; Mw=36632  [identification data]

IDENTIFICATION: SPOT 648: Peptides number=6. SeqCov=17.7%. MOWSE score=5.71E+4 [1]
SPOT 653: Peptides number=8. SeqCov=30.2%. MOWSE score=4.54E+8 [1]; SPOT 1096: Peptides number=7. SeqCov=22.5%. MOWSE score=9.70E+6 [1].
MAPPING (identification):
SPOT 648: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 653: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 1096: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright
Data from Dr. Willard Freeman, Penn State College of Medicine, USA
Cross-references
UniProtKB/Swiss-ProtO88989; MDHC_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_12
Entry nameMDHC_RAT
Primary accession numberO88989
Secondary accession number(s) O88585 Q6PCV2
Sequence was last modified on January 23, 2007 (version 3)
Annotations were last modified on November 16, 2011 (version 84)
Name and origin of the protein
DescriptionRecName: Full=Malate dehydrogenase, cytoplasmic; EC=1.1.1.37; AltName: Full=Cytosolic malate dehydrogenase;
Gene nameName=Mdh1
Synonyms=Mdh
Encoded onName=Mdh1; Synonyms=Mdh
KeywordsAcetylation; Complete proteome; Cytoplasm; Direct protein sequencing; NAD; Oxidoreductase; Phosphoprotein; Reference proteome; Tricarboxylic acid cycle; Ubl conjugation.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLAF093773; AAC64180.1; -; mRNA
EMBLBC059124; AAH59124.1; -; mRNA
EMBLAF075574; AAC26799.1; -; mRNA
IPIIPI00198717; -; .
RefSeqNP_150238.1; NM_033235.1; .
UniGeneRn.13492; -; .
ProteinModelPortalO88989; -; .
SMRO88989; 2-334; .
IntActO88989; 1; .
STRINGO88989; -; .
PhosphoSiteO88989; -; .
World-2DPAGE0004:O88989; -; .
PRIDEO88989; -; .
EnsemblENSRNOT00000011429; ENSRNOP00000011429; ENSRNOG00000008103; .
GeneID24551; -; .
KEGGrno:24551; -; .
NMPDRfig|10116.3.peg.10844; -; .
UCSCNM_033235; rat; .
CTD4190; -; .
RGD3072; Mdh1; .
eggNOGroNOG11216; -; .
GeneTreeENSGT00530000063410; -; .
HOVERGENHBG006340; -; .
InParanoidO88989; -; .
OrthoDBEOG4CVG78; -; .
PhylomeDBO88989; -; .
NextBio603654; -; .
ArrayExpressO88989; -; .
GenevestigatorO88989; -; .
GermOnlineENSRNOG00000008103; Rattus norvegicus; .
GOGO:0005625; C:soluble fraction; IDA:RGD; .
GOGO:0030060; F:L-malate dehydrogenase activity; IDA:RGD; .
GOGO:0051287; F:NAD binding; IDA:RGD; .
GOGO:0044262; P:cellular carbohydrate metabolic process; IEA:InterPro; .
GOGO:0006108; P:malate metabolic process; IDA:RGD; .
GOGO:0006734; P:NADH metabolic process; IDA:RGD; .
GOGO:0006107; P:oxaloacetate metabolic process; IDA:RGD; .
GOGO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW; .
InterProIPR001557; L-lactate/malate_DH; .
InterProIPR022383; Lactate/malate_DH_C; .
InterProIPR001236; Lactate/malate_DH_N; .
InterProIPR015955; Lactate_DH/Glyco_Ohase_4_C; .
InterProIPR001252; Malate_DH_AS; .
InterProIPR011274; Malate_DH_NAD-dep_euk; .
InterProIPR010945; Malate_DH_type2; .
InterProIPR016040; NAD(P)-bd_dom; .
Gene3DG3DSA:3.90.110.10; lact_mal_DH; 1; .
Gene3DG3DSA:3.40.50.720; NAD(P)-bd; 1; .
KOK00025; -; .
PANTHERPTHR23382; MDH_SF1; 1; .
PfamPF02866; Ldh_1_C; 1; .
PfamPF00056; Ldh_1_N; 1; .
PIRSFPIRSF000102; Lac_mal_DH; 1; .
SUPFAMSSF56327; Lactate_DH/Glyco_hydro_4_C; 1; .
TIGRFAMsTIGR01759; MalateDH-SF1; 1; .
TIGRFAMsTIGR01758; MDH_euk_cyt; 1; .
PROSITEPS00068; MDH; 1; .





Searching in 'World-2DPAGE Repository [0022]' for entry matching: O88989




World-2DPAGE Repository (0022):  O88989

O88989


General information about the entry
View entry in simple text format
Entry nameMDHC_RAT
Primary accession numberO88989
integrated into World-2DPAGE Repository (0022) on May 7, 2010 (release 1)
2D Annotations were last modified onJune 22, 2011 (version 2)
General Annotations were last modified on November 24, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Malate dehydrogenase, cytoplasmic; EC=1.1.1.37; AltName: Full=Cytosolic malate dehydrogenase;.
Gene nameName=Mdh1
Synonyms=Mdh
Annotated speciesRattus [TaxID: 10114]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000593;
Burniston J.G., Kenyani J., Wastling J.M., Burant C.F., Qi N.R., Koch L.G., Britton S.L.
''Proteomic analysis reveals perturbed energy metabolism and elevated oxidative stress in hearts of rats with inborn low aerobic capacity''
Proteomics 11(16):3369-3379 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

HCR_LCR_HEART {HCR LCR Cardiac Map}
Rattus
Tissue: Heart
HCR_LCR_HEART
  map experimental info 		 
HCR_LCR_HEART

MAP LOCATIONS:
pI=6.16; Mw=36631  [identification data]
pI=6.16; Mw=36631  [identification data]
pI=6.16; Mw=36631  [identification data]
pI=6.16; Mw=36631  [identification data]

IDENTIFICATION: SPOT 348: SeqCov=37.7%. Peptides MS=12/20. Mascot PMF Score=103 [1]
SPOT 440: SeqCov=27.2%. Peptides MS=9/20. Mascot PMF Score=63 [1]; SPOT 513: SeqCov=32%. Peptides MS=10/20. Mascot PMF Score=77 [1]; SPOT 514: SeqCov=40.1%. Peptides MS=14/20. Mascot PMF Score=122 [1].
PEPTIDE EVIDENCE: SPOT 348: 221-GEFITTVQQR-230 (68) / 299-FVEGLPINDFSR-310 (77) / 180-NVIIWGNHSSTQYPDVNHAK-199 (95) [1]
SPOT 440: 221-GEFITTVQQR-230 (62) / 299-FVEGLPINDFSR-310 (88) [1]; SPOT 513: 221-GEFITTVQQR-230 (49) / 299-FVEGLPINDFSR-310 (69) [1].
MAPPING (identification):
SPOT 348: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 440: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 513: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1];
SPOT 514: Peptide mass fingerprinting [1].

Copyright
Data from Dr. Jatin Burniston, Liverpool John Moores University, UK
Cross-references
UniProtKB/Swiss-ProtO88989; MDHC_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameMDHC_RAT
Primary accession numberO88989
Secondary accession number(s) O88585 Q6PCV2
Sequence was last modified on January 23, 2007 (version 3)
Annotations were last modified on November 16, 2011 (version 84)
Name and origin of the protein
DescriptionRecName: Full=Malate dehydrogenase, cytoplasmic; EC=1.1.1.37; AltName: Full=Cytosolic malate dehydrogenase;
Gene nameName=Mdh1
Synonyms=Mdh
Encoded onName=Mdh1; Synonyms=Mdh
KeywordsAcetylation; Complete proteome; Cytoplasm; Direct protein sequencing; NAD; Oxidoreductase; Phosphoprotein; Reference proteome; Tricarboxylic acid cycle; Ubl conjugation.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLAF093773; AAC64180.1; -; mRNA
EMBLBC059124; AAH59124.1; -; mRNA
EMBLAF075574; AAC26799.1; -; mRNA
IPIIPI00198717; -; .
RefSeqNP_150238.1; NM_033235.1; .
UniGeneRn.13492; -; .
ProteinModelPortalO88989; -; .
SMRO88989; 2-334; .
IntActO88989; 1; .
STRINGO88989; -; .
PhosphoSiteO88989; -; .
World-2DPAGE0004:O88989; -; .
PRIDEO88989; -; .
EnsemblENSRNOT00000011429; ENSRNOP00000011429; ENSRNOG00000008103; .
GeneID24551; -; .
KEGGrno:24551; -; .
NMPDRfig|10116.3.peg.10844; -; .
UCSCNM_033235; rat; .
CTD4190; -; .
RGD3072; Mdh1; .
eggNOGroNOG11216; -; .
GeneTreeENSGT00530000063410; -; .
HOVERGENHBG006340; -; .
InParanoidO88989; -; .
OrthoDBEOG4CVG78; -; .
PhylomeDBO88989; -; .
NextBio603654; -; .
ArrayExpressO88989; -; .
GenevestigatorO88989; -; .
GermOnlineENSRNOG00000008103; Rattus norvegicus; .
GOGO:0005625; C:soluble fraction; IDA:RGD; .
GOGO:0030060; F:L-malate dehydrogenase activity; IDA:RGD; .
GOGO:0051287; F:NAD binding; IDA:RGD; .
GOGO:0044262; P:cellular carbohydrate metabolic process; IEA:InterPro; .
GOGO:0006108; P:malate metabolic process; IDA:RGD; .
GOGO:0006734; P:NADH metabolic process; IDA:RGD; .
GOGO:0006107; P:oxaloacetate metabolic process; IDA:RGD; .
GOGO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW; .
InterProIPR001557; L-lactate/malate_DH; .
InterProIPR022383; Lactate/malate_DH_C; .
InterProIPR001236; Lactate/malate_DH_N; .
InterProIPR015955; Lactate_DH/Glyco_Ohase_4_C; .
InterProIPR001252; Malate_DH_AS; .
InterProIPR011274; Malate_DH_NAD-dep_euk; .
InterProIPR010945; Malate_DH_type2; .
InterProIPR016040; NAD(P)-bd_dom; .
Gene3DG3DSA:3.90.110.10; lact_mal_DH; 1; .
Gene3DG3DSA:3.40.50.720; NAD(P)-bd; 1; .
KOK00025; -; .
PANTHERPTHR23382; MDH_SF1; 1; .
PfamPF02866; Ldh_1_C; 1; .
PfamPF00056; Ldh_1_N; 1; .
PIRSFPIRSF000102; Lac_mal_DH; 1; .
SUPFAMSSF56327; Lactate_DH/Glyco_hydro_4_C; 1; .
TIGRFAMsTIGR01759; MalateDH-SF1; 1; .
TIGRFAMsTIGR01758; MDH_euk_cyt; 1; .
PROSITEPS00068; MDH; 1; .





Searching in 'World-2DPAGE Repository [0030]' for entry matching: O88989




World-2DPAGE Repository (0030):  O88989

O88989


General information about the entry
View entry in simple text format
Entry nameMDHC_RAT
Primary accession numberO88989
integrated into World-2DPAGE Repository (0030) on November 9, 2010 (release 1)
2D Annotations were last modified onJune 21, 2011 (version 2)
General Annotations were last modified on November 23, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Malate dehydrogenase, cytoplasmic; EC=1.1.1.37; AltName: Full=Cytosolic malate dehydrogenase;.
Gene nameName=Mdh1
Synonyms=Mdh
Annotated speciesRattus norvegicus (Rat) [TaxID: 10116]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/pmic.201000006;
D'Hertog W., Maris M., Thorrez L., Waelkens E., Overbergh L., Mathieu C.
''Two-dimensional gel proteome reference map of INS-1E cells''
Proteomics 11(7):1365-1369 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

RAT_INS1E_4-7 {INS-1E cell line, a model rat cell line for pancreatic beta cells, PH4-7}
Rattus norvegicus (Rat)
RAT_INS1E_4-7
  map experimental info 		 
RAT_INS1E_4-7

MAP LOCATIONS:
pI=6.19; Mw=42735  [identification data]
pI=6.21; Mw=40884  [identification data]
pI=6.17; Mw=40044  [identification data]

IDENTIFICATION: SPOT 1608: Mascot protein score=45. Number of peptides=2 [1]
SPOT 1678: Mascot protein score=82. Number of peptides=2 [1]; SPOT 1702: Mascot protein score=170. Number of peptides=4 [1].
MAPPING (identification):
SPOT 1608: Tandem mass spectrometry [1];
SPOT 1678: Tandem mass spectrometry [1];
SPOT 1702: Tandem mass spectrometry [1].

Copyright
Data from Dr. Wannes D'Hertog, Catholic University Leuven, Belgium
Cross-references
UniProtKB/Swiss-ProtO88989; MDHC_RAT.
World-2DPAGE RepositoryO88989; MDHC_RAT.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameMDHC_RAT
Primary accession numberO88989
Secondary accession number(s) O88585 Q6PCV2
Sequence was last modified on January 23, 2007 (version 3)
Annotations were last modified on November 16, 2011 (version 84)
Name and origin of the protein
DescriptionRecName: Full=Malate dehydrogenase, cytoplasmic; EC=1.1.1.37; AltName: Full=Cytosolic malate dehydrogenase;
Gene nameName=Mdh1
Synonyms=Mdh
Encoded onName=Mdh1; Synonyms=Mdh
KeywordsAcetylation; Complete proteome; Cytoplasm; Direct protein sequencing; NAD; Oxidoreductase; Phosphoprotein; Reference proteome; Tricarboxylic acid cycle; Ubl conjugation.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLAF093773; AAC64180.1; -; mRNA
EMBLBC059124; AAH59124.1; -; mRNA
EMBLAF075574; AAC26799.1; -; mRNA
IPIIPI00198717; -; .
RefSeqNP_150238.1; NM_033235.1; .
UniGeneRn.13492; -; .
ProteinModelPortalO88989; -; .
SMRO88989; 2-334; .
IntActO88989; 1; .
STRINGO88989; -; .
PhosphoSiteO88989; -; .
World-2DPAGE0004:O88989; -; .
PRIDEO88989; -; .
EnsemblENSRNOT00000011429; ENSRNOP00000011429; ENSRNOG00000008103; .
GeneID24551; -; .
KEGGrno:24551; -; .
NMPDRfig|10116.3.peg.10844; -; .
UCSCNM_033235; rat; .
CTD4190; -; .
RGD3072; Mdh1; .
eggNOGroNOG11216; -; .
GeneTreeENSGT00530000063410; -; .
HOVERGENHBG006340; -; .
InParanoidO88989; -; .
OrthoDBEOG4CVG78; -; .
PhylomeDBO88989; -; .
NextBio603654; -; .
ArrayExpressO88989; -; .
GenevestigatorO88989; -; .
GermOnlineENSRNOG00000008103; Rattus norvegicus; .
GOGO:0005625; C:soluble fraction; IDA:RGD; .
GOGO:0030060; F:L-malate dehydrogenase activity; IDA:RGD; .
GOGO:0051287; F:NAD binding; IDA:RGD; .
GOGO:0044262; P:cellular carbohydrate metabolic process; IEA:InterPro; .
GOGO:0006108; P:malate metabolic process; IDA:RGD; .
GOGO:0006734; P:NADH metabolic process; IDA:RGD; .
GOGO:0006107; P:oxaloacetate metabolic process; IDA:RGD; .
GOGO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW; .
InterProIPR001557; L-lactate/malate_DH; .
InterProIPR022383; Lactate/malate_DH_C; .
InterProIPR001236; Lactate/malate_DH_N; .
InterProIPR015955; Lactate_DH/Glyco_Ohase_4_C; .
InterProIPR001252; Malate_DH_AS; .
InterProIPR011274; Malate_DH_NAD-dep_euk; .
InterProIPR010945; Malate_DH_type2; .
InterProIPR016040; NAD(P)-bd_dom; .
Gene3DG3DSA:3.90.110.10; lact_mal_DH; 1; .
Gene3DG3DSA:3.40.50.720; NAD(P)-bd; 1; .
KOK00025; -; .
PANTHERPTHR23382; MDH_SF1; 1; .
PfamPF02866; Ldh_1_C; 1; .
PfamPF00056; Ldh_1_N; 1; .
PIRSFPIRSF000102; Lac_mal_DH; 1; .
SUPFAMSSF56327; Lactate_DH/Glyco_hydro_4_C; 1; .
TIGRFAMsTIGR01759; MalateDH-SF1; 1; .
TIGRFAMsTIGR01758; MDH_euk_cyt; 1; .
PROSITEPS00068; MDH; 1; .





Searching in 'World-2DPAGE Repository [0001]' for entry matching: O88989




World-2DPAGE Repository (0001):  O88989

This protein does not exist in the current release of World-2DPAGE Repository (0001)



Searching in 'World-2DPAGE Repository [0002]' for entry matching: O88989




World-2DPAGE Repository (0002):  O88989

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Searching in 'World-2DPAGE Repository [0003]' for entry matching: O88989




World-2DPAGE Repository (0003):  O88989

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Searching in 'World-2DPAGE Repository [0005]' for entry matching: O88989




World-2DPAGE Repository (0005):  O88989

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Searching in 'World-2DPAGE Repository [0006]' for entry matching: O88989




World-2DPAGE Repository (0006):  O88989

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Searching in 'World-2DPAGE Repository [0007]' for entry matching: O88989




World-2DPAGE Repository (0007):  O88989

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World-2DPAGE Repository (0008):  O88989

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World-2DPAGE Repository (0009):  O88989

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Searching in 'World-2DPAGE Repository [0010]' for entry matching: O88989




World-2DPAGE Repository (0010):  O88989

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World-2DPAGE Repository (0011):  O88989

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World-2DPAGE Repository (0012):  O88989

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Searching in 'World-2DPAGE Repository [0017]' for entry matching: O88989




World-2DPAGE Repository (0017):  O88989

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World-2DPAGE Repository (0018):  O88989

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Searching in 'World-2DPAGE Repository [0020]' for entry matching: O88989




World-2DPAGE Repository (0020):  O88989

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Searching in 'World-2DPAGE Repository [0021]' for entry matching: O88989




World-2DPAGE Repository (0021):  O88989

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Searching in 'World-2DPAGE Repository [0023]' for entry matching: O88989




World-2DPAGE Repository (0023):  O88989

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Searching in 'World-2DPAGE Repository [0024]' for entry matching: O88989




World-2DPAGE Repository (0024):  O88989

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Searching in 'World-2DPAGE Repository [0026]' for entry matching: O88989




World-2DPAGE Repository (0026):  O88989

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Searching in 'World-2DPAGE Repository [0027]' for entry matching: O88989




World-2DPAGE Repository (0027):  O88989

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Searching in 'World-2DPAGE Repository [0029]' for entry matching: O88989




World-2DPAGE Repository (0029):  O88989

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Searching in 'World-2DPAGE Repository [0031]' for entry matching: O88989




World-2DPAGE Repository (0031):  O88989

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Searching in 'World-2DPAGE Repository [0032]' for entry matching: O88989




World-2DPAGE Repository (0032):  O88989

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Searching in 'World-2DPAGE Repository [0034]' for entry matching: O88989




World-2DPAGE Repository (0034):  O88989

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Searching in 'World-2DPAGE Repository [0035]' for entry matching: O88989




World-2DPAGE Repository (0035):  O88989

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Searching in 'World-2DPAGE Repository [0036]' for entry matching: O88989




World-2DPAGE Repository (0036):  O88989

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Searching in 'World-2DPAGE Repository [0037]' for entry matching: O88989




World-2DPAGE Repository (0037):  O88989

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Searching in 'World-2DPAGE Repository [0038]' for entry matching: O88989




World-2DPAGE Repository (0038):  O88989

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Searching in 'World-2DPAGE Repository [0039]' for entry matching: O88989




World-2DPAGE Repository (0039):  O88989

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Searching in 'World-2DPAGE Repository [0040]' for entry matching: O88989




World-2DPAGE Repository (0040):  O88989

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Searching in 'World-2DPAGE Repository [0042]' for entry matching: O88989




World-2DPAGE Repository (0042):  O88989

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Searching in 'World-2DPAGE Repository [0043]' for entry matching: O88989




World-2DPAGE Repository (0043):  O88989

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Searching in 'World-2DPAGE Repository [0044]' for entry matching: O88989




World-2DPAGE Repository (0044):  O88989

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Searching in 'World-2DPAGE Repository [0046]' for entry matching: O88989




World-2DPAGE Repository (0046):  O88989

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Searching in 'World-2DPAGE Repository [0047]' for entry matching: O88989




World-2DPAGE Repository (0047):  O88989

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Searching in 'World-2DPAGE Repository [0050]' for entry matching: O88989




World-2DPAGE Repository (0050):  O88989

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Searching in 'World-2DPAGE Repository [0051]' for entry matching: O88989




World-2DPAGE Repository (0051):  O88989

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Searching in 'World-2DPAGE Repository [0053]' for entry matching: O88989




World-2DPAGE Repository (0053):  O88989

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Searching in 'World-2DPAGE Repository [0054]' for entry matching: O88989




World-2DPAGE Repository (0054):  O88989

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Searching in 'World-2DPAGE Repository [0056]' for entry matching: O88989




World-2DPAGE Repository (0056):  O88989

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Searching in 'World-2DPAGE Repository [0059]' for entry matching: O88989




World-2DPAGE Repository (0059):  O88989

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Searching in 'World-2DPAGE Repository [0061]' for entry matching: O88989




World-2DPAGE Repository (0061):  O88989

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Searching in 'World-2DPAGE Repository [0062]' for entry matching: O88989




World-2DPAGE Repository (0062):  O88989

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Searching in 'World-2DPAGE Repository [0063]' for entry matching: O88989




World-2DPAGE Repository (0063):  O88989

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Searching in 'World-2DPAGE Repository [0066]' for entry matching: O88989




World-2DPAGE Repository (0066):  O88989

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Searching in 'World-2DPAGE Repository [0068]' for entry matching: O88989




World-2DPAGE Repository (0068):  O88989

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Searching in 'World-2DPAGE Repository [0069]' for entry matching: O88989




World-2DPAGE Repository (0069):  O88989

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Searching in 'World-2DPAGE Repository [0076]' for entry matching: O88989




World-2DPAGE Repository (0076):  O88989

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World-2DPAGE Repository (0077):  O88989

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Searching in 'World-2DPAGE Repository [0080]' for entry matching: O88989




World-2DPAGE Repository (0080):  O88989

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Searching in 'World-2DPAGE Repository [0084]' for entry matching: O88989




World-2DPAGE Repository (0084):  O88989

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Searching in 'World-2DPAGE Repository [0085]' for entry matching: O88989




World-2DPAGE Repository (0085):  O88989

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Searching in 'World-2DPAGE Repository [0088]' for entry matching: O88989




World-2DPAGE Repository (0088):  O88989

This protein does not exist in the current release of World-2DPAGE Repository (0088)



World-2DPAGE Repository image

World-2DPAGE Repository (search AC)

Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the Expasy web server