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Searching in 'World-2DPAGE Repository [0027]' for entry matching: P20152




World-2DPAGE Repository (0027):  P20152


P20152


General information about the entry
View entry in simple text format
Entry nameVIME_MOUSE
Primary accession numberP20152
integrated into World-2DPAGE Repository (0027) on September 30, 2010 (release 1)
2D Annotations were last modified onJune 21, 2011 (version 2)
General Annotations were last modified on November 23, 2011 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Vimentin;.
Gene nameName=Vim
Annotated speciesMus musculus (Mouse) [TaxID: 10090]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus; Mus.
References
[1]   2D-PAGE GEL CHARACTERIZATION
DOI=10.1002/prca.201000103;
Blutke A., Block C., Berendt F., Herbach N., Kemter E., Amann K., Frolich T., Arnold G.J., Wanke R.
''Differential glomerular proteome analysis of two murine nephropathy models at onset of albuminuria''
Proteomics - Clinical Applications 5(6):375-381 (2011)
2D PAGE maps for identified proteins
How to interpret a protein

GIPRDN_4-7 {glomerular protein lysates of a GIPRdn-tg animal}
Mus musculus (Mouse)
GIPRDN_4-7
  map experimental info
 
GIPRDN_4-7

MAP LOCATIONS:
pI=4.71; Mw=45000  [identification data]

EXPRESSION:
SPOT 14: Avg ratio GIPRdn-tg vs. control=1.67 (p-value=0.0094) [1].

IDENTIFICATION: SPOT 14: SeqCov=73%. Mascot protein score=789 [1].
MAPPING (identification):
SPOT 14: Peptide mass fingerprinting [1]; Tandem mass spectrometry [1].

Copyright
Data from Dr. Andreas Blutke, Institute of Veterinary Pathology, University Munich, Germany
Cross-references
REPRODUCTION-2DPAGEIPI00227299; IPI00227299.
SWISS-2DPAGEP20152; VIME_MOUSE.
UCD-2DPAGEP20152; VIME_MOUSE.
UniProtKB/Swiss-ProtP20152; VIME_MOUSE.
World-2DPAGE RepositoryP20152; VIME_MOUSE.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2011_11
Entry nameVIME_MOUSE
Primary accession numberP20152
Secondary accession number(s) O08704 Q8CCH1
Sequence was last modified on January 23, 2007 (version 3)
Annotations were last modified on November 16, 2011 (version 128)
Name and origin of the protein
DescriptionRecName: Full=Vimentin;
Gene nameName=Vim
Encoded onName=Vim
KeywordsAcetylation; Coiled coil; Complete proteome; Cytoplasm; Direct protein sequencing; Intermediate filament; Phosphoprotein; Reference proteome.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM24849; AAA40555.1; -; mRNA
EMBLX56397; CAA39807.1; -; mRNA
EMBLM26251; AAA40556.1; -; mRNA
EMBLZ22526; CAA80251.1; -; Genomic_DNA
EMBLX51438; CAA35803.1; -; mRNA
EMBLY07738; CAA69019.1; -; Genomic_DNA
EMBLAK033175; BAC28181.1; -; mRNA
EMBLD50805; BAA19834.1; -; Genomic_DNA
IPIIPI00227299; -; .
PIRA43803; A43803; .
RefSeqNP_035831.2; NM_011701.4; .
UniGeneMm.268000; -; .
ProteinModelPortalP20152; -; .
SMRP20152; 101-138; 141-248; 264-406
DIPDIP-188N; -; .
IntActP20152; 10; .
MINTMINT-1202726; -; .
STRINGP20152; -; .
PhosphoSiteP20152; -; .
SWISS-2DPAGEP20152; -; .
REPRODUCTION-2DPAGEIPI00227299; -; .
UCD-2DPAGEP20152; -; .
PRIDEP20152; -; .
EnsemblENSMUST00000028062; ENSMUSP00000028062; ENSMUSG00000026728; .
GeneID22352; -; .
KEGGmmu:22352; -; .
CTD7431; -; .
MGIMGI:98932; Vim; .
HOGENOMHBG715391; -; .
HOVERGENHBG013015; -; .
InParanoidP20152; -; .
OMAANRTNEK; -; .
OrthoDBEOG4GHZPD; -; .
PhylomeDBP20152; -; .
NextBio302643; -; .
ArrayExpressP20152; -; .
BgeeP20152; -; .
CleanExMM_VIM; -; .
GenevestigatorP20152; -; .
GermOnlineENSMUSG00000026728; Mus musculus; .
GOGO:0031252; C:cell leading edge; IDA:MGI; .
GOGO:0045098; C:type III intermediate filament; TAS:MGI; .
GOGO:0005212; F:structural constituent of eye lens; IDA:MGI; .
GOGO:0014002; P:astrocyte development; IGI:MGI; .
GOGO:0060020; P:Bergmann glial cell differentiation; IMP:MGI; .
GOGO:0045109; P:intermediate filament organization; IGI:MGI; .
GOGO:0070307; P:lens fiber cell development; IDA:MGI; .
GOGO:0010977; P:negative regulation of neuron projection development; IGI:MGI; .
InterProIPR016044; F; .
InterProIPR001664; IF; .
InterProIPR006821; Intermed_filament_DNA-bd; .
InterProIPR018039; Intermediate_filament_CS; .
KOK07606; -; .
PANTHERPTHR23239; IF; 1; .
PfamPF00038; Filament; 1; .
PfamPF04732; Filament_head; 1; .
PROSITEPS00226; IF; 1; .





Searching in 'World-2DPAGE Repository [0043]' for entry matching: P20152




World-2DPAGE Repository (0043):  P20152


P20152


General information about the entry
View entry in simple text format
Entry nameVIME_MOUSE
Primary accession numberP20152
integrated into World-2DPAGE Repository (0043) on January 4, 2012 (release 1)
2D Annotations were last modified onJanuary 6, 2012 (version 1)
General Annotations were last modified on April 18, 2012 (version 2)
Name and origin of the protein
DescriptionRecName: Full=Vimentin;.
Gene nameName=Vim
Annotated speciesMus musculus (Mouse) [TaxID: 10090]
TaxonomyEukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus; Mus.
References
[1]   2D GEL CHARACTERIZATION
DOI=10.1016/j.jprot.2012.04.001 ;
Sun Z., Biela L.M., Hamilton K.L., Reardon K.F.
''Concentration-dependent effects of the soy phytoestrogen genistein on the proteome of cultured cardiomyocytes''
Journal of Proteomics 0(0):0-0 (2012)
2D PAGE maps for identified proteins
How to interpret a protein

MMUSCULUS_HL-1PHI_4-7 {mouse HL-1 cultured cardiomyocytes hydrophilic extraction}
Mus musculus (Mouse)
Tissue: Cardiac myocyte
MMUSCULUS_HL-1PHI_4-7
  map experimental info
 
MMUSCULUS_HL-1PHI_4-7

MAP LOCATIONS:
Mw=53712  [identification data]
Mw=53712  [identification data]

%COV: BAND phi18: 11.1 [1]
BAND phi57: 19 [1].
GENEID: BAND phi18: VIME_MOUSE [1]
BAND phi57: VIME_MOUSE [1].
SCORE: BAND phi18: 271 [1]
BAND phi57: 679 [1].
SEARCH ENGINE: BAND phi18: Mascot [1]
BAND phi57: Mascot [1].
MAPPING (identification):
BAND phi18: Tandem mass spectrometry [1];
BAND phi57: Tandem mass spectrometry [1].



MMUSCULUS_HL-1PHO_4-7 {mouse HL-1 cultured cardiomyocytes hydrophobic extraction}
Mus musculus (Mouse)
Tissue: Cardiac myocyte
MMUSCULUS_HL-1PHO_4-7
  map experimental info
 
MMUSCULUS_HL-1PHO_4-7

MAP LOCATIONS:
Mw=53712  [identification data]
Mw=53712  [identification data]

%COV: BAND pho13: 10.6 [1]
BAND pho41: 5.56 [1].
GENEID: BAND pho13: VIME_MOUSE [1]
BAND pho41: VIME_MOUSE [1].
SCORE: BAND pho13: 211 [1]
BAND pho41: 132 [1].
SEARCH ENGINE: BAND pho13: Mascot [1]
BAND pho41: Mascot [1].
MAPPING (identification):
BAND pho13: Tandem mass spectrometry [1];
BAND pho41: Tandem mass spectrometry [1].

Copyright
Data from Dr. Zeyu Sun, Department of Chemical and Biological Engineering, Colorado State University, Fort Collins, USA
Cross-references
IPI00227299.
UniProtKB/Swiss-ProtP20152; VIME_MOUSE.



2D PAGE maps for identified proteins
  • How to interpret a protein map
  • You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw.
  • Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly.
  • Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.



External data extracted from UniProtKB/Swiss-Prot
Extracted from UniProtKB/Swiss-Prot, release: 2012_03
Entry nameVIME_MOUSE
Primary accession numberP20152
Secondary accession number(s) O08704 Q8CCH1
Sequence was last modified on January 23, 2007 (version 3)
Annotations were last modified on March 21, 2012 (version 132)
Name and origin of the protein
DescriptionRecName: Full=Vimentin;
Gene nameName=Vim
Encoded onName=Vim
KeywordsAcetylation; Coiled coil; Complete proteome; Cytoplasm; Direct protein sequencing; Intermediate filament; Phosphoprotein; Reference proteome.
Copyright
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License
Cross-references
EMBLM24849; AAA40555.1; -; mRNA
EMBLX56397; CAA39807.1; -; mRNA
EMBLM26251; AAA40556.1; -; mRNA
EMBLZ22526; CAA80251.1; -; Genomic_DNA
EMBLX51438; CAA35803.1; -; mRNA
EMBLY07738; CAA69019.1; -; Genomic_DNA
EMBLAK033175; BAC28181.1; -; mRNA
EMBLD50805; BAA19834.1; -; Genomic_DNA
IPIIPI00227299; -; .
PIRA43803; A43803; .
RefSeqNP_035831.2; NM_011701.4; .
UniGeneMm.268000; -; .
ProteinModelPortalP20152; -; .
SMRP20152; 101-138; 144-248; 263-406
DIPDIP-188N; -; .
IntActP20152; 10; .
MINTMINT-1202726; -; .
STRINGP20152; -; .
PhosphoSiteP20152; -; .
SWISS-2DPAGEP20152; -; .
REPRODUCTION-2DPAGEIPI00227299; -; .
UCD-2DPAGEP20152; -; .
PRIDEP20152; -; .
EnsemblENSMUST00000028062; ENSMUSP00000028062; ENSMUSG00000026728; .
GeneID22352; -; .
KEGGmmu:22352; -; .
CTD7431; -; .
MGIMGI:98932; Vim; .
eggNOGNOG146769; -; .
GeneTreeENSGT00560000076592; -; .
HOGENOMHBG715391; -; .
HOVERGENHBG013015; -; .
InParanoidP20152; -; .
KOK07606; -; .
OMAANRTNEK; -; .
OrthoDBEOG4GHZPD; -; .
PhylomeDBP20152; -; .
NextBio302643; -; .
ArrayExpressP20152; -; .
BgeeP20152; -; .
CleanExMM_VIM; -; .
GenevestigatorP20152; -; .
GermOnlineENSMUSG00000026728; Mus musculus; .
GOGO:0031252; C:cell leading edge; IDA:MGI; .
GOGO:0045098; C:type III intermediate filament; TAS:MGI; .
GOGO:0005212; F:structural constituent of eye lens; IDA:MGI; .
GOGO:0014002; P:astrocyte development; IGI:MGI; .
GOGO:0060020; P:Bergmann glial cell differentiation; IMP:MGI; .
GOGO:0045109; P:intermediate filament organization; IGI:MGI; .
GOGO:0070307; P:lens fiber cell development; IDA:MGI; .
GOGO:0010977; P:negative regulation of neuron projection development; IGI:MGI; .
InterProIPR016044; F; .
InterProIPR001664; IF; .
InterProIPR006821; Intermed_filament_DNA-bd; .
InterProIPR018039; Intermediate_filament_CS; .
PANTHERPTHR23239; IF; 1; .
PfamPF00038; Filament; 1; .
PfamPF04732; Filament_head; 1; .
PROSITEPS00226; IF; 1; .





Searching in 'World-2DPAGE Repository [0001]' for entry matching: P20152




World-2DPAGE Repository (0001):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0001)



Searching in 'World-2DPAGE Repository [0002]' for entry matching: P20152




World-2DPAGE Repository (0002):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0002)



Searching in 'World-2DPAGE Repository [0003]' for entry matching: P20152




World-2DPAGE Repository (0003):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0003)



Searching in 'World-2DPAGE Repository [0004]' for entry matching: P20152




World-2DPAGE Repository (0004):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0004)



Searching in 'World-2DPAGE Repository [0005]' for entry matching: P20152




World-2DPAGE Repository (0005):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0005)



Searching in 'World-2DPAGE Repository [0006]' for entry matching: P20152




World-2DPAGE Repository (0006):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0006)



Searching in 'World-2DPAGE Repository [0007]' for entry matching: P20152




World-2DPAGE Repository (0007):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0007)



Searching in 'World-2DPAGE Repository [0008]' for entry matching: P20152




World-2DPAGE Repository (0008):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0008)



Searching in 'World-2DPAGE Repository [0009]' for entry matching: P20152




World-2DPAGE Repository (0009):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0009)



Searching in 'World-2DPAGE Repository [0010]' for entry matching: P20152




World-2DPAGE Repository (0010):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0010)



Searching in 'World-2DPAGE Repository [0011]' for entry matching: P20152




World-2DPAGE Repository (0011):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0011)



Searching in 'World-2DPAGE Repository [0012]' for entry matching: P20152




World-2DPAGE Repository (0012):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0012)



Searching in 'World-2DPAGE Repository [0014]' for entry matching: P20152




World-2DPAGE Repository (0014):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0014)



Searching in 'World-2DPAGE Repository [0017]' for entry matching: P20152




World-2DPAGE Repository (0017):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0017)



Searching in 'World-2DPAGE Repository [0018]' for entry matching: P20152




World-2DPAGE Repository (0018):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0018)



Searching in 'World-2DPAGE Repository [0019]' for entry matching: P20152




World-2DPAGE Repository (0019):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0019)



Searching in 'World-2DPAGE Repository [0020]' for entry matching: P20152




World-2DPAGE Repository (0020):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0020)



Searching in 'World-2DPAGE Repository [0021]' for entry matching: P20152




World-2DPAGE Repository (0021):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0021)



Searching in 'World-2DPAGE Repository [0022]' for entry matching: P20152




World-2DPAGE Repository (0022):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0022)



Searching in 'World-2DPAGE Repository [0023]' for entry matching: P20152




World-2DPAGE Repository (0023):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0023)



Searching in 'World-2DPAGE Repository [0024]' for entry matching: P20152




World-2DPAGE Repository (0024):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0024)



Searching in 'World-2DPAGE Repository [0026]' for entry matching: P20152




World-2DPAGE Repository (0026):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0026)



Searching in 'World-2DPAGE Repository [0029]' for entry matching: P20152




World-2DPAGE Repository (0029):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0029)



Searching in 'World-2DPAGE Repository [0030]' for entry matching: P20152




World-2DPAGE Repository (0030):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0030)



Searching in 'World-2DPAGE Repository [0031]' for entry matching: P20152




World-2DPAGE Repository (0031):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0031)



Searching in 'World-2DPAGE Repository [0032]' for entry matching: P20152




World-2DPAGE Repository (0032):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0032)



Searching in 'World-2DPAGE Repository [0034]' for entry matching: P20152




World-2DPAGE Repository (0034):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0034)



Searching in 'World-2DPAGE Repository [0035]' for entry matching: P20152




World-2DPAGE Repository (0035):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0035)



Searching in 'World-2DPAGE Repository [0036]' for entry matching: P20152




World-2DPAGE Repository (0036):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0036)



Searching in 'World-2DPAGE Repository [0037]' for entry matching: P20152




World-2DPAGE Repository (0037):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0037)



Searching in 'World-2DPAGE Repository [0038]' for entry matching: P20152




World-2DPAGE Repository (0038):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0038)



Searching in 'World-2DPAGE Repository [0039]' for entry matching: P20152




World-2DPAGE Repository (0039):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0039)



Searching in 'World-2DPAGE Repository [0040]' for entry matching: P20152




World-2DPAGE Repository (0040):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0040)



Searching in 'World-2DPAGE Repository [0042]' for entry matching: P20152




World-2DPAGE Repository (0042):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0042)



Searching in 'World-2DPAGE Repository [0044]' for entry matching: P20152




World-2DPAGE Repository (0044):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0044)



Searching in 'World-2DPAGE Repository [0046]' for entry matching: P20152




World-2DPAGE Repository (0046):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0046)



Searching in 'World-2DPAGE Repository [0047]' for entry matching: P20152




World-2DPAGE Repository (0047):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0047)



Searching in 'World-2DPAGE Repository [0050]' for entry matching: P20152




World-2DPAGE Repository (0050):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0050)



Searching in 'World-2DPAGE Repository [0051]' for entry matching: P20152




World-2DPAGE Repository (0051):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0051)



Searching in 'World-2DPAGE Repository [0053]' for entry matching: P20152




World-2DPAGE Repository (0053):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0053)



Searching in 'World-2DPAGE Repository [0054]' for entry matching: P20152




World-2DPAGE Repository (0054):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0054)



Searching in 'World-2DPAGE Repository [0056]' for entry matching: P20152




World-2DPAGE Repository (0056):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0056)



Searching in 'World-2DPAGE Repository [0059]' for entry matching: P20152




World-2DPAGE Repository (0059):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0059)



Searching in 'World-2DPAGE Repository [0061]' for entry matching: P20152




World-2DPAGE Repository (0061):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0061)



Searching in 'World-2DPAGE Repository [0062]' for entry matching: P20152




World-2DPAGE Repository (0062):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0062)



Searching in 'World-2DPAGE Repository [0063]' for entry matching: P20152




World-2DPAGE Repository (0063):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0063)



Searching in 'World-2DPAGE Repository [0066]' for entry matching: P20152




World-2DPAGE Repository (0066):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0066)



Searching in 'World-2DPAGE Repository [0068]' for entry matching: P20152




World-2DPAGE Repository (0068):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0068)



Searching in 'World-2DPAGE Repository [0069]' for entry matching: P20152




World-2DPAGE Repository (0069):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0069)



Searching in 'World-2DPAGE Repository [0076]' for entry matching: P20152




World-2DPAGE Repository (0076):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0076)



Searching in 'World-2DPAGE Repository [0077]' for entry matching: P20152




World-2DPAGE Repository (0077):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0077)



Searching in 'World-2DPAGE Repository [0080]' for entry matching: P20152




World-2DPAGE Repository (0080):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0080)



Searching in 'World-2DPAGE Repository [0084]' for entry matching: P20152




World-2DPAGE Repository (0084):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0084)



Searching in 'World-2DPAGE Repository [0085]' for entry matching: P20152




World-2DPAGE Repository (0085):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0085)



Searching in 'World-2DPAGE Repository [0088]' for entry matching: P20152




World-2DPAGE Repository (0088):  P20152

This protein does not exist in the current release of World-2DPAGE Repository (0088)




World-2DPAGE Repository image

World-2DPAGE Repository (search AC)


Database constructed and maintained by SIB, using the Make2D-DB II package (ver. 3.10.2) from the World-2DPAGE Constellation of the ExPASy web server