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Sample Preparation and Post-separation Analysis Searching in 'SWISS-2DPAGE' for entry matching: P09147 SWISS-2DPAGE:  P09147 P09147 General information about the entry View entry in simple text format Entry name GALE_ECOLI Primary accession number P09147 integrated into SWISS-2DPAGE on  May 15, 2003 (release 16) 2D Annotations were last modified on May 15, 2003 (version 1) General Annotations were last modified on  May 19, 2011 (version 6) Name and origin of the protein Description RecName: Full=UDP-glucose 4-epimerase; EC=5.1.3.2; AltName: Full=Galactowaldenase; AltName: Full=UDP-galactose 4-epimerase;. Gene name Name=galE Synonyms=galD OrderedLocusNames=b0759, JW0742 Annotated species Escherichia coli [TaxID: 562] Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia. References [1]   MAPPING ON GEL PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan] Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S. ''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';'' Proteomics 2(1):1682-1698(2002) Comments SUBUNIT: HOMODIMER 2D PAGE maps for identified proteins How to interpret a protein ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)} Escherichia coli   map experimental info   protein estimated location   ECOLI-DIGE4.5-6.5 MAP LOCATIONS: SPOT 2D-001WOQ: pI=5.91; Mw=35787   [identification data] EXPRESSION: increase after benzoic acid treatment [1]. MAPPING (identification): Tandem mass spectrometry [1]. Copyright This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss). Cross-references 2DBase-Ecoli P09147; GALE_ECOLI. UniProtKB/Swiss-Prot P09147; GALE_ECOLI. 2D PAGE maps for identified proteins How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed. External data extracted from UniProtKB/Swiss-Prot Extracted from UniProtKB/Swiss-Prot, release: 2011_10 Entry name GALE_ECOLI Primary accession number P09147 Secondary accession number(s) Q47493 Sequence was last modified on  July 1, 1989 (version 1) Annotations were last modified on  October 19, 2011 (version 113) Name and origin of the protein Description RecName: Full=UDP-glucose 4-epimerase; EC=5.1.3.2; AltName: Full=Galactowaldenase; AltName: Full=UDP-galactose 4-epimerase; Gene name Name=galE Synonyms=galD OrderedLocusNames=b0759, JW0742 Encoded on Name=galE; Synonyms=galD; OrderedLocusNames=b0759, JW0742 Keywords 3D-structure; Carbohydrate metabolism; Complete proteome; Galactose metabolism; Isomerase; NAD; Reference proteome. Copyright Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License Cross-references EMBL X06226; CAA29573.1; -; Genomic_DNA EMBL U00096; AAC73846.1; -; Genomic_DNA EMBL AP009048; BAA35421.1; -; Genomic_DNA EMBL X51449; CAA35813.1; -; Genomic_DNA EMBL U07867; AAB06890.1; -; Genomic_DNA EMBL J01613; AAA87978.1; -; Genomic_DNA PIR S02089; XUECUG; . RefSeq NP_415280.3; NC_000913.2; . PDB 1A9Y; X-ray; 1.80 A; A=1-338 PDB 1A9Z; X-ray; 1.90 A; A=1-338 PDB 1KVQ; X-ray; 2.15 A; A=1-338 PDB 1KVR; X-ray; 1.90 A; A=1-338 PDB 1KVS; X-ray; 2.15 A; A=1-338 PDB 1KVT; X-ray; 2.15 A; A=1-338 PDB 1KVU; X-ray; 1.90 A; A=1-338 PDB 1LRJ; X-ray; 1.90 A; A=1-338 PDB 1LRK; X-ray; 1.75 A; A=1-338 PDB 1LRL; X-ray; 1.80 A; A=1-338 PDB 1NAH; X-ray; 1.80 A; A=1-338 PDB 1NAI; X-ray; 2.00 A; A=1-338 PDB 1UDA; X-ray; 1.80 A; A=1-338 PDB 1UDB; X-ray; 1.65 A; A=1-338 PDB 1UDC; X-ray; 1.65 A; A=1-338 PDB 1XEL; X-ray; 1.80 A; A=1-338 PDB 2UDP; X-ray; 1.80 A; A/B=1-338 PDBsum 1A9Y; -; . PDBsum 1A9Z; -; . PDBsum 1KVQ; -; . PDBsum 1KVR; -; . PDBsum 1KVS; -; . PDBsum 1KVT; -; . PDBsum 1KVU; -; . PDBsum 1LRJ; -; . PDBsum 1LRK; -; . PDBsum 1LRL; -; . PDBsum 1NAH; -; . PDBsum 1NAI; -; . PDBsum 1UDA; -; . PDBsum 1UDB; -; . PDBsum 1UDC; -; . PDBsum 1XEL; -; . PDBsum 2UDP; -; . ProteinModelPortal P09147; -; . SMR P09147; 1-338; . DIP DIP-9728N; -; . IntAct P09147; 3; . SWISS-2DPAGE P09147; -; . 2DBase-Ecoli P09147; -; . EnsemblBacteria EBESCT00000004431; EBESCP00000004431; EBESCG00000003616; . EnsemblBacteria EBESCT00000004432; EBESCP00000004432; EBESCG00000003616; . EnsemblBacteria EBESCT00000004433; EBESCP00000004433; EBESCG00000003616; . EnsemblBacteria EBESCT00000014770; EBESCP00000014061; EBESCG00000013830; . GeneID 945354; -; . GenomeReviews AP009048_GR; JW0742; . GenomeReviews U00096_GR; b0759; . KEGG ecj:JW0742; -; . KEGG eco:b0759; -; . EchoBASE EB0357; -; . EcoGene EG10362; galE; . eggNOG COG1087; -; . GeneTree EBGT00050000008809; -; . HOGENOM HBG755066; -; . OMA PYQESFP; -; . ProtClustDB PRK10675; -; . BioCyc EcoCyc:UDPGLUCEPIM-MONOMER; -; . BioCyc MetaCyc:UDPGLUCEPIM-MONOMER; -; . Genevestigator P09147; -; . GO GO:0005829; C:cytosol; IDA:UniProtKB; . GO GO:0070403; F:NAD+ binding; IDA:EcoCyc; . GO GO:0003978; F:UDP-glucose 4-epimerase activity; IDA:EcoCyc; . GO GO:0009242; P:colanic acid biosynthetic process; IMP:EcoCyc; . GO GO:0033499; P:galactose catabolic process via UDP-galactose; IMP:EcoCyc; . InterPro IPR001509; Epimerase_deHydtase; . InterPro IPR005886; GalE; . InterPro IPR016040; NAD(P)-bd_dom; . Gene3D G3DSA:3.40.50.720; NAD(P)-bd; 1; . PANTHER PTHR10366:SF39; GalE; 1; . Pfam PF01370; Epimerase; 1; . TIGRFAMs TIGR01179; GalE; 1; . SWISS-2DPAGE (search AC)