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Sample Preparation and Post-separation Analysis Searching in 'SWISS-2DPAGE' for entry matching: P0A7A9 SWISS-2DPAGE:  P0A7A9 P0A7A9 General information about the entry View entry in simple text format Entry name IPYR_ECOLI Primary accession number P0A7A9 Secondary accession number(s) P17288 integrated into SWISS-2DPAGE on  September 1, 1997 (release 6) 2D Annotations were last modified on May 15, 2003 (version 3) General Annotations were last modified on  May 19, 2011 (version 14) Name and origin of the protein Description RecName: Full=Inorganic pyrophosphatase; EC=3.6.1.1; AltName: Full=Pyrophosphate phospho-hydrolase; Short=PPase;. Gene name Name=ppa OrderedLocusNames=b4226, JW4185 Annotated species Escherichia coli [TaxID: 562] Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia. References [1]   MAPPING ON GEL MEDLINE=98410772; PubMed=9740056; [NCBI, Expasy, EBI, Israel, Japan] Tonella L., Walsh B.J., Sanchez J.-C., Ou K., Wilkins M.R., Tyler M., Frutiger S., Gooley A.A., Pescaru I., Appel R.D., Yan J.X., Bairoch A., Hoogland C., Morch F.S., Hughes G.J., Williams K.L., Hochstrasser D.F. '''''''98 Escherichia coli SWISS-2DPAGE database update'';'';'' Electrophoresis 19(1):1960-1971(1998) [2]   MAPPING ON GEL PubMed=11680886; [NCBI, Expasy, EBI, Israel, Japan] Tonella L., Hoogland C., Binz P.-A., Appel R.D., Hochstrasser D.F., Sanchez J.-C. ''''''New perspectives in the Escherichia coli proteome investigation'';'';'' Proteomics 1(1):409-423(2001) [3]   MAPPING ON GEL PubMed=12469338; [NCBI, Expasy, EBI, Israel, Japan] Yan J.X., Devenish A.T., Wait R., Stone T., Lewis S., Fowler S. ''''''Fluorescence 2-D difference gel electrophoresis and mass spectrometry based proteomic analysis of E. coli'';'';'' Proteomics 2(1):1682-1698(2002) 2D PAGE maps for identified proteins How to interpret a protein ECOLI {Escherichia coli} Escherichia coli   map experimental info   protein estimated location   ECOLI MAP LOCATIONS: SPOT 2D-000LKZ: pI=5.01; Mw=21579 MAPPING (identification): AMINO ACID COMPOSITION AND SEQUENCE TAG (SLLN) [1]. ECOLI4.5-5.5 {Escherichia coli(4.5-5.5)} Escherichia coli   map experimental info   protein estimated location   ECOLI4.5-5.5 MAP LOCATIONS: SPOT 2D-0015D7: pI=5.06; Mw=21653   [identification data] MAPPING (identification): Peptide mass fingerprinting [2], and microsequencing (SLLN) [2]. ECOLI-DIGE4.5-6.5 {Escherichia coli DIGE (4.5-6.5)} Escherichia coli   map experimental info   protein estimated location   ECOLI-DIGE4.5-6.5 MAP LOCATIONS: SPOT 2D-001WV8: pI=5.00; Mw=22748   [identification data] MAPPING (identification): Peptide mass fingerprinting [3]. Copyright This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://world-2dpage.expasy.org/swiss-2dpage/docs/license.html or send email from legal@sib.swiss). Cross-references 2DBase-Ecoli P0A7A9; IPYR_ECOLI. UniProtKB/Swiss-Prot P0A7A9; IPYR_ECOLI. 2D PAGE maps for identified proteins How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed. External data extracted from UniProtKB/Swiss-Prot Extracted from UniProtKB/Swiss-Prot, release: 2011_10 Entry name IPYR_ECOLI Primary accession number P0A7A9 Secondary accession number(s) P17288 Q2M679 Sequence was last modified on  January 23, 2007 (version 2) Annotations were last modified on  October 19, 2011 (version 62) Name and origin of the protein Description RecName: Full=Inorganic pyrophosphatase; EC=3.6.1.1; AltName: Full=Pyrophosphate phospho-hydrolase; Short=PPase; Gene name Name=ppa OrderedLocusNames=b4226, JW4185 Encoded on Name=ppa; OrderedLocusNames=b4226, JW4185 Keywords 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Hydrolase; Magnesium; Metal-binding; Reference proteome. Copyright Copyrighted by the UniProt Consortium, see https://www.uniprot.org/help/license. Distributed under the Creative Commons Attribution-NoDerivs License Cross-references EMBL M23550; AAB88583.1; -; Unassigned_DNA EMBL U14003; AAA97123.1; -; Genomic_DNA EMBL U00096; AAC77183.1; -; Genomic_DNA EMBL AP009048; BAE78227.1; -; Genomic_DNA PIR A27648; PWEC; . RefSeq NP_418647.1; NC_000913.2; . PDB 1FAJ; X-ray; 2.15 A; A=2-176 PDB 1I40; X-ray; 1.10 A; A=2-175 PDB 1I6T; X-ray; 1.20 A; A=2-175 PDB 1IGP; X-ray; 2.20 A; A=2-175 PDB 1INO; X-ray; 2.20 A; A=2-175 PDB 1IPW; X-ray; 2.30 A; A/B=2-176 PDB 1JFD; X-ray; 2.20 A; A/B=2-176 PDB 1MJW; X-ray; 1.95 A; A/B=2-175 PDB 1MJX; X-ray; 2.15 A; A/B=2-175 PDB 1MJY; X-ray; 2.10 A; A/B=2-175 PDB 1MJZ; X-ray; 2.20 A; A=2-175 PDB 1OBW; X-ray; 1.90 A; A/B/C=2-176 PDB 2AU6; X-ray; 1.20 A; A=2-175 PDB 2AU7; X-ray; 1.05 A; A=2-175 PDB 2AU8; X-ray; 1.65 A; A=2-175 PDB 2AU9; X-ray; 1.30 A; A=2-175 PDB 2AUU; X-ray; 1.22 A; A=2-175 PDB 2EIP; X-ray; 2.20 A; A/B=2-176 PDBsum 1FAJ; -; . PDBsum 1I40; -; . PDBsum 1I6T; -; . PDBsum 1IGP; -; . PDBsum 1INO; -; . PDBsum 1IPW; -; . PDBsum 1JFD; -; . PDBsum 1MJW; -; . PDBsum 1MJX; -; . PDBsum 1MJY; -; . PDBsum 1MJZ; -; . PDBsum 1OBW; -; . PDBsum 2AU6; -; . PDBsum 2AU7; -; . PDBsum 2AU8; -; . PDBsum 2AU9; -; . PDBsum 2AUU; -; . PDBsum 2EIP; -; . ProteinModelPortal P0A7A9; -; . SMR P0A7A9; 2-176; . DIP DIP-36217N; -; . IntAct P0A7A9; 36; . MINT MINT-1226708; -; . PhosSite P0A7A9; -; . SWISS-2DPAGE P0A7A9; -; . 2DBase-Ecoli P0A7A9; -; . EnsemblBacteria EBESCT00000003037; EBESCP00000003037; EBESCG00000002490; . EnsemblBacteria EBESCT00000015331; EBESCP00000014622; EBESCG00000014391; . GeneID 948748; -; . GenomeReviews AP009048_GR; JW4185; . GenomeReviews U00096_GR; b4226; . KEGG ecj:JW4185; -; . KEGG eco:b4226; -; . EchoBASE EB0748; -; . EcoGene EG10755; ppa; . eggNOG COG0221; -; . GeneTree EBGT00050000009133; -; . HOGENOM HBG529150; -; . OMA AMVIMEE; -; . ProtClustDB PRK01250; -; . BioCyc EcoCyc:INORGPYROPHOSPHAT-MONOMER; -; . BioCyc MetaCyc:INORGPYROPHOSPHAT-MONOMER; -; . Genevestigator P0A7A9; -; . GO GO:0005829; C:cytosol; IDA:UniProtKB; . GO GO:0016020; C:membrane; IDA:UniProtKB; . GO GO:0004427; F:inorganic diphosphatase activity; IEA:EC; . GO GO:0000287; F:magnesium ion binding; IEA:InterPro; . GO GO:0008270; F:zinc ion binding; IDA:EcoliWiki; . GO GO:0006796; P:phosphate-containing compound metabolic process; IEA:InterPro; . HAMAP MF_00209; Inorganic_PPase; 1; - InterPro IPR008162; Pyrophosphatase; . Gene3D G3DSA:3.90.80.10; Pyrophosphatase; 1; . PANTHER PTHR10286; Pyrophosphatase; 1; . Pfam PF00719; Pyrophosphatase; 1; . SUPFAM SSF50324; Pyrophosphatase; 1; . PROSITE PS00387; PPASE; 1; . SWISS-2DPAGE (search AC)